Bacillus phage phIS3501

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Camtrevirus; Bacillus virus phIS3501

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0USV1|H0USV1_9CAUD Uncharacterized protein OS=Bacillus phage phIS3501 OX=1124578 GN=phIS3501_020 PE=4 SV=1
MM1 pKa = 7.61SLLQTLLEE9 pKa = 4.74TINQEE14 pKa = 3.65WSSQSTDD21 pKa = 2.88VFGEE25 pKa = 3.84VDD27 pKa = 3.62NVLSIAYY34 pKa = 8.28IAPVLAKK41 pKa = 10.36YY42 pKa = 10.26IEE44 pKa = 4.33SEE46 pKa = 4.03GDD48 pKa = 3.3KK49 pKa = 11.07VPTEE53 pKa = 3.92FEE55 pKa = 4.42PNKK58 pKa = 10.36FNSDD62 pKa = 3.31KK63 pKa = 10.8EE64 pKa = 4.38GNYY67 pKa = 7.9YY68 pKa = 9.08TVEE71 pKa = 3.94IKK73 pKa = 10.84VCTDD77 pKa = 2.56QDD79 pKa = 3.96TLIEE83 pKa = 4.64FYY85 pKa = 11.41YY86 pKa = 10.57EE87 pKa = 3.75

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0USV2|H0USV2_9CAUD Uncharacterized protein OS=Bacillus phage phIS3501 OX=1124578 GN=phIS3501_021 PE=4 SV=1
MM1 pKa = 7.64HH2 pKa = 6.93EE3 pKa = 4.41RR4 pKa = 11.84IFRR7 pKa = 11.84FAKK10 pKa = 9.69NKK12 pKa = 10.12KK13 pKa = 8.32STFEE17 pKa = 3.73QCSFPEE23 pKa = 4.59VYY25 pKa = 10.06IAWTCKK31 pKa = 9.94RR32 pKa = 11.84FLRR35 pKa = 11.84NKK37 pKa = 8.4MNKK40 pKa = 9.25LAPEE44 pKa = 3.94RR45 pKa = 11.84KK46 pKa = 9.38KK47 pKa = 10.65PYY49 pKa = 10.82NNII52 pKa = 3.36

Molecular weight: 6.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11158

52

1343

218.8

25.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.166 ± 0.628

0.789 ± 0.155

5.53 ± 0.257

8.451 ± 0.298

4.069 ± 0.266

5.449 ± 0.403

1.694 ± 0.205

7.501 ± 0.316

9.339 ± 0.291

7.86 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.029 ± 0.179

5.888 ± 0.214

2.787 ± 0.296

4.445 ± 0.267

4.517 ± 0.222

5.637 ± 0.262

5.799 ± 0.242

6.148 ± 0.211

1.147 ± 0.146

3.755 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski