Charadrius vociferus (Killdeer) (Aegialitis vocifera)
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14257 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0AKE6|A0A0A0AKE6_CHAVO Uncharacterized protein (Fragment) OS=Charadrius vociferus OX=50402 GN=N301_03777 PE=4 SV=1
DDD2 pKa = 5.37 DDD4 pKa = 3.8 VAFCVLATDDD14 pKa = 4.65 EE15 pKa = 4.49 EE16 pKa = 4.94 DD17 pKa = 3.58 EE18 pKa = 5.25 DDD20 pKa = 3.36 ALQIHHH26 pKa = 6.13 TLIQAFCCEEE36 pKa = 3.78 DDD38 pKa = 2.96 DDD40 pKa = 3.95 VRR42 pKa = 11.84 VNDDD46 pKa = 3.46 AKKK49 pKa = 10.32 AAIVGPSEEE58 pKa = 4.11 EE59 pKa = 4.26 GEEE62 pKa = 4.11 RR63 pKa = 11.84 DDD65 pKa = 3.68 HHH67 pKa = 6.98 ILITV
Molecular weight: 7.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.808
IPC_protein 3.745
Toseland 3.541
ProMoST 3.973
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.872
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A0A0AX11|A0A0A0AX11_CHAVO Sulfhydryl oxidase (Fragment) OS=Charadrius vociferus OX=50402 GN=N301_12484 PE=3 SV=1
DDD2 pKa = 4.68 KK3 pKa = 10.96 RR4 pKa = 11.84 QGKKK8 pKa = 6.62 RR9 pKa = 11.84 QGKKK13 pKa = 6.2 RR14 pKa = 11.84 QGKKK18 pKa = 6.2 RR19 pKa = 11.84 QGKKK23 pKa = 6.2 RR24 pKa = 11.84 QGKKK28 pKa = 6.2 RR29 pKa = 11.84 QGKKK33 pKa = 6.2 RR34 pKa = 11.84 QGKKK38 pKa = 6.2 RR39 pKa = 11.84 QGKKK43 pKa = 6.19 RR44 pKa = 11.84 QGKKK48 pKa = 8.58 RR49 pKa = 11.84 QGKKK53 pKa = 8.76 RR54 pKa = 11.84 QGKKK58 pKa = 6.51 RR59 pKa = 11.84
Molecular weight: 6.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.544
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.266
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.04
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14257
0
14257
6061522
32
5559
425.2
47.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.424 ± 0.018
2.253 ± 0.02
5.032 ± 0.017
7.179 ± 0.026
3.928 ± 0.014
5.9 ± 0.025
2.543 ± 0.011
4.992 ± 0.015
6.43 ± 0.022
9.74 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.236 ± 0.01
4.153 ± 0.014
5.257 ± 0.027
4.627 ± 0.018
5.281 ± 0.019
8.104 ± 0.026
5.382 ± 0.015
6.346 ± 0.017
1.205 ± 0.008
2.986 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here