Dolphin rhabdovirus
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068EWX6|A0A068EWX6_9RHAB Nucleocapsid protein OS=Dolphin rhabdovirus OX=1511639 PE=4 SV=1
MM1 pKa = 7.14 MMTRR5 pKa = 11.84 RR6 pKa = 11.84 PLKK9 pKa = 10.23 IEE11 pKa = 3.74 YY12 pKa = 11.04 DD13 pKa = 3.48 MDD15 pKa = 3.89 KK16 pKa = 11.41 LEE18 pKa = 4.79 ASLKK22 pKa = 10.13 EE23 pKa = 3.7 ADD25 pKa = 4.39 EE26 pKa = 4.5 IEE28 pKa = 4.4 GANEE32 pKa = 4.64 GISEE36 pKa = 4.21 RR37 pKa = 11.84 MSDD40 pKa = 3.54 LKK42 pKa = 11.17 SLNEE46 pKa = 4.14 SLDD49 pKa = 3.76 DD50 pKa = 5.89 LLAEE54 pKa = 5.43 DD55 pKa = 5.14 DD56 pKa = 3.62 WAEE59 pKa = 3.85 QGGIEE64 pKa = 4.29 TDD66 pKa = 3.44 AEE68 pKa = 4.33 ADD70 pKa = 4.03 DD71 pKa = 4.03 EE72 pKa = 4.7 HH73 pKa = 7.07 EE74 pKa = 4.53 SAVKK78 pKa = 10.73 SSDD81 pKa = 2.88 VDD83 pKa = 3.69 MNPSDD88 pKa = 4.79 DD89 pKa = 5.13 AWDD92 pKa = 3.69 SDD94 pKa = 4.5 CFVPPDD100 pKa = 3.22 IPGYY104 pKa = 6.29 EE105 pKa = 3.83 TRR107 pKa = 11.84 GYY109 pKa = 10.46 KK110 pKa = 10.17 LSNSITPATRR120 pKa = 11.84 LAILKK125 pKa = 8.22 EE126 pKa = 3.85 VSKK129 pKa = 10.63 IIEE132 pKa = 4.37 DD133 pKa = 3.61 LRR135 pKa = 11.84 GDD137 pKa = 3.6 MVIAPKK143 pKa = 10.78 GDD145 pKa = 3.26 TYY147 pKa = 11.37 DD148 pKa = 3.73 YY149 pKa = 11.59 YY150 pKa = 12.06 LMFPDD155 pKa = 6.14 GYY157 pKa = 11.05 FNNKK161 pKa = 9.39 DD162 pKa = 3.5 VGRR165 pKa = 11.84 KK166 pKa = 9.0 HH167 pKa = 6.12 YY168 pKa = 9.96 PGDD171 pKa = 4.54 RR172 pKa = 11.84 IPPTDD177 pKa = 4.8 DD178 pKa = 3.64 PPGADD183 pKa = 3.64 KK184 pKa = 10.82 TPEE187 pKa = 3.91 GLKK190 pKa = 8.85 TRR192 pKa = 11.84 KK193 pKa = 9.87 NGANDD198 pKa = 3.47 DD199 pKa = 3.78 KK200 pKa = 11.34 NRR202 pKa = 11.84 RR203 pKa = 11.84 SVKK206 pKa = 9.45 EE207 pKa = 3.98 ANATAQKK214 pKa = 10.38 SNKK217 pKa = 8.81 LASEE221 pKa = 3.58 IWEE224 pKa = 4.22 TGVVVIAKK232 pKa = 9.89 DD233 pKa = 3.76 GKK235 pKa = 10.59 SKK237 pKa = 10.79 LRR239 pKa = 11.84 LKK241 pKa = 10.2 PDD243 pKa = 2.93 KK244 pKa = 10.78 LYY246 pKa = 10.34 WEE248 pKa = 4.0 RR249 pKa = 11.84 VEE251 pKa = 5.71 WFKK254 pKa = 11.15 QWDD257 pKa = 4.06 EE258 pKa = 4.82 KK259 pKa = 11.7 NMEE262 pKa = 4.19 TLPRR266 pKa = 11.84 SKK268 pKa = 10.03 IIQHH272 pKa = 7.0 LIRR275 pKa = 11.84 KK276 pKa = 6.84 TPMRR280 pKa = 11.84 LTYY283 pKa = 9.17 RR284 pKa = 11.84 RR285 pKa = 11.84 KK286 pKa = 10.47 YY287 pKa = 10.09 ILEE290 pKa = 4.01
Molecular weight: 33.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.935
IPC2_protein 4.889
IPC_protein 4.838
Toseland 4.685
ProMoST 4.902
Dawson 4.787
Bjellqvist 4.952
Wikipedia 4.673
Rodwell 4.673
Grimsley 4.596
Solomon 4.787
Lehninger 4.736
Nozaki 4.889
DTASelect 5.08
Thurlkill 4.685
EMBOSS 4.685
Sillero 4.952
Patrickios 4.342
IPC_peptide 4.787
IPC2_peptide 4.94
IPC2.peptide.svr19 4.942
Protein with the highest isoelectric point:
>tr|A0A068EQH8|A0A068EQH8_9RHAB GDP polyribonucleotidyltransferase OS=Dolphin rhabdovirus OX=1511639 PE=4 SV=1
MM1 pKa = 7.39 FKK3 pKa = 10.73 SKK5 pKa = 10.66 ALSLFRR11 pKa = 11.84 KK12 pKa = 7.14 TPKK15 pKa = 10.42 DD16 pKa = 2.89 KK17 pKa = 11.08 TMNRR21 pKa = 11.84 KK22 pKa = 9.52 DD23 pKa = 3.3 KK24 pKa = 11.22 SPVKK28 pKa = 10.23 RR29 pKa = 11.84 VMKK32 pKa = 10.02 YY33 pKa = 10.66 NEE35 pKa = 4.07 VADD38 pKa = 4.24 SEE40 pKa = 5.31 DD41 pKa = 3.61 GFPLFKK47 pKa = 10.37 PIPEE51 pKa = 4.05 GTYY54 pKa = 9.98 YY55 pKa = 10.8 RR56 pKa = 11.84 HH57 pKa = 6.04 ITLKK61 pKa = 11.03 VNFKK65 pKa = 10.78 LSIVTKK71 pKa = 10.26 EE72 pKa = 3.91 PIEE75 pKa = 4.0 NLMEE79 pKa = 4.62 IYY81 pKa = 10.8 LIGQHH86 pKa = 6.9 ILDD89 pKa = 4.81 NYY91 pKa = 10.0 NGPARR96 pKa = 11.84 SKK98 pKa = 10.81 PMYY101 pKa = 9.21 LALMIGGFNGLEE113 pKa = 4.23 KK114 pKa = 10.51 SHH116 pKa = 4.79 QTSRR120 pKa = 11.84 ITCYY124 pKa = 9.42 EE125 pKa = 4.05 RR126 pKa = 11.84 EE127 pKa = 3.97 FHH129 pKa = 7.0 GPIDD133 pKa = 3.63 FPYY136 pKa = 10.34 YY137 pKa = 10.3 RR138 pKa = 11.84 RR139 pKa = 11.84 DD140 pKa = 3.93 PIDD143 pKa = 3.44 WVIMPLSTAYY153 pKa = 8.54 EE154 pKa = 4.37 CSMKK158 pKa = 10.61 GHH160 pKa = 6.36 TEE162 pKa = 3.83 VTVYY166 pKa = 11.25 ASLTPTNMTGPSLAQFCEE184 pKa = 3.86 GHH186 pKa = 5.84 PRR188 pKa = 11.84 MRR190 pKa = 11.84 KK191 pKa = 8.98 PPILDD196 pKa = 3.14 TLTNFKK202 pKa = 10.31 IDD204 pKa = 3.32 TKK206 pKa = 10.92 LKK208 pKa = 9.47 NEE210 pKa = 3.92 EE211 pKa = 3.58 WHH213 pKa = 7.38 LDD215 pKa = 3.42 MSQCC219 pKa = 4.75
Molecular weight: 25.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.349
IPC2_protein 8.302
IPC_protein 8.229
Toseland 8.712
ProMoST 8.712
Dawson 9.077
Bjellqvist 9.019
Wikipedia 9.194
Rodwell 9.224
Grimsley 8.99
Solomon 9.194
Lehninger 9.165
Nozaki 9.136
DTASelect 8.902
Thurlkill 9.004
EMBOSS 9.224
Sillero 9.194
Patrickios 4.482
IPC_peptide 9.18
IPC2_peptide 7.746
IPC2.peptide.svr19 7.687
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3550
219
2086
710.0
81.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.62 ± 0.731
1.465 ± 0.275
5.859 ± 0.739
6.592 ± 0.648
4.0 ± 0.435
5.662 ± 0.402
2.535 ± 0.513
7.211 ± 0.404
6.873 ± 0.534
9.803 ± 1.114
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.268 ± 0.406
5.099 ± 0.517
4.704 ± 0.419
2.986 ± 0.299
5.296 ± 0.481
7.606 ± 0.316
5.775 ± 0.905
5.268 ± 0.426
1.746 ± 0.109
3.634 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here