Porcine stool-associated circular virus 5
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8T0A7|W8T0A7_9CIRC Cap protein OS=Porcine stool-associated circular virus 5 OX=1475063 PE=4 SV=1
MM1 pKa = 7.83 SSVRR5 pKa = 11.84 LQNKK9 pKa = 9.18 NLFLTYY15 pKa = 10.36 NDD17 pKa = 3.9 PEE19 pKa = 4.19 EE20 pKa = 4.9 TITGPAWLLNKK31 pKa = 10.0 LAEE34 pKa = 4.23 QLEE37 pKa = 4.83 KK38 pKa = 10.02 YY39 pKa = 8.97 QPYY42 pKa = 10.49 YY43 pKa = 10.31 GVCGEE48 pKa = 4.56 EE49 pKa = 4.09 KK50 pKa = 10.87 APTTGTKK57 pKa = 9.67 HH58 pKa = 4.06 YY59 pKa = 10.09 HH60 pKa = 5.66 LLIMCKK66 pKa = 10.25 NPVISKK72 pKa = 9.11 NGQVIEE78 pKa = 4.21 IKK80 pKa = 10.74 GIAPHH85 pKa = 6.66 IEE87 pKa = 4.07 KK88 pKa = 10.15 IRR90 pKa = 11.84 NNLRR94 pKa = 11.84 GVIKK98 pKa = 10.24 YY99 pKa = 9.02 VKK101 pKa = 9.91 KK102 pKa = 10.66 DD103 pKa = 3.39 GNIAEE108 pKa = 4.61 LNKK111 pKa = 10.25 NLCPVKK117 pKa = 10.35 LDD119 pKa = 3.22 KK120 pKa = 11.36 LEE122 pKa = 4.45 RR123 pKa = 11.84 KK124 pKa = 9.25 EE125 pKa = 4.62 KK126 pKa = 10.59 ADD128 pKa = 4.43 LMLTGDD134 pKa = 4.24 LEE136 pKa = 4.2 KK137 pKa = 11.19 AFIDD141 pKa = 3.97 GTLGPIEE148 pKa = 4.36 VIRR151 pKa = 11.84 AEE153 pKa = 4.12 KK154 pKa = 9.94 LRR156 pKa = 11.84 KK157 pKa = 9.13 IFQVYY162 pKa = 9.66 RR163 pKa = 11.84 KK164 pKa = 9.41 PEE166 pKa = 4.36 PYY168 pKa = 9.81 SKK170 pKa = 10.81 KK171 pKa = 10.35 LIMWYY176 pKa = 10.03 RR177 pKa = 11.84 GEE179 pKa = 4.09 TGEE182 pKa = 4.3 GKK184 pKa = 8.25 TRR186 pKa = 11.84 TAVEE190 pKa = 3.79 LAEE193 pKa = 4.2 EE194 pKa = 4.71 FEE196 pKa = 4.13 LSYY199 pKa = 10.49 WITNEE204 pKa = 3.64 SLRR207 pKa = 11.84 WFDD210 pKa = 3.85 GYY212 pKa = 11.34 NGQEE216 pKa = 4.07 LVIIDD221 pKa = 4.61 DD222 pKa = 4.64 FRR224 pKa = 11.84 QKK226 pKa = 10.46 MITDD230 pKa = 3.42 WNFLLRR236 pKa = 11.84 LLDD239 pKa = 4.3 GYY241 pKa = 10.89 GLSVPVKK248 pKa = 9.81 GGYY251 pKa = 7.9 VAWKK255 pKa = 8.61 PKK257 pKa = 10.15 IIIITTPATPQEE269 pKa = 4.04 AFQWMNKK276 pKa = 10.08 DD277 pKa = 3.78 GEE279 pKa = 4.36 LQNWDD284 pKa = 4.32 HH285 pKa = 7.3 LDD287 pKa = 3.28 QLMRR291 pKa = 11.84 RR292 pKa = 11.84 LTHH295 pKa = 7.24 DD296 pKa = 4.53 DD297 pKa = 3.74 EE298 pKa = 4.67 EE299 pKa = 4.46 QVYY302 pKa = 8.46 QFPLWEE308 pKa = 4.12 EE309 pKa = 4.29 DD310 pKa = 3.27 KK311 pKa = 11.09 QKK313 pKa = 11.27 LMNTVRR319 pKa = 11.84 QFLGIEE325 pKa = 3.78 NDD327 pKa = 3.17 EE328 pKa = 4.33 AMVEE332 pKa = 4.48 EE333 pKa = 4.78 EE334 pKa = 5.04 LSMSPILPEE343 pKa = 3.62 PTQIDD348 pKa = 3.83 EE349 pKa = 4.25 AA350 pKa = 5.31
Molecular weight: 40.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.232
IPC2_protein 5.308
IPC_protein 5.245
Toseland 5.207
ProMoST 5.372
Dawson 5.219
Bjellqvist 5.347
Wikipedia 5.067
Rodwell 5.156
Grimsley 5.13
Solomon 5.219
Lehninger 5.181
Nozaki 5.347
DTASelect 5.461
Thurlkill 5.194
EMBOSS 5.13
Sillero 5.423
Patrickios 4.266
IPC_peptide 5.232
IPC2_peptide 5.423
IPC2.peptide.svr19 5.447
Protein with the highest isoelectric point:
>tr|W8T0A7|W8T0A7_9CIRC Cap protein OS=Porcine stool-associated circular virus 5 OX=1475063 PE=4 SV=1
MM1 pKa = 7.66 PKK3 pKa = 9.89 RR4 pKa = 11.84 YY5 pKa = 9.8 ASRR8 pKa = 11.84 KK9 pKa = 5.78 RR10 pKa = 11.84 TYY12 pKa = 9.69 RR13 pKa = 11.84 KK14 pKa = 9.43 KK15 pKa = 10.03 SRR17 pKa = 11.84 KK18 pKa = 5.72 ITRR21 pKa = 11.84 FRR23 pKa = 11.84 RR24 pKa = 11.84 YY25 pKa = 8.68 RR26 pKa = 11.84 TGTRR30 pKa = 11.84 AKK32 pKa = 9.35 AKK34 pKa = 10.11 SYY36 pKa = 10.48 FRR38 pKa = 11.84 LRR40 pKa = 11.84 RR41 pKa = 11.84 AAKK44 pKa = 9.98 NYY46 pKa = 9.92 SKK48 pKa = 10.73 SRR50 pKa = 11.84 KK51 pKa = 8.26 IKK53 pKa = 9.43 RR54 pKa = 11.84 AKK56 pKa = 9.78 RR57 pKa = 11.84 QYY59 pKa = 10.92 KK60 pKa = 7.73 YY61 pKa = 9.22 TAGKK65 pKa = 9.52 GVKK68 pKa = 9.83 NKK70 pKa = 9.79 IKK72 pKa = 10.31 RR73 pKa = 11.84 LTMSEE78 pKa = 4.3 FAQLPFYY85 pKa = 10.84 DD86 pKa = 3.63 IARR89 pKa = 11.84 LSSMVMSGGNPNMYY103 pKa = 10.26 PNIPDD108 pKa = 4.36 NIRR111 pKa = 11.84 SAYY114 pKa = 9.27 KK115 pKa = 10.64 GQFGPYY121 pKa = 6.78 WSRR124 pKa = 11.84 YY125 pKa = 7.41 GAILPEE131 pKa = 4.44 EE132 pKa = 4.42 YY133 pKa = 10.51 FNASSPYY140 pKa = 10.63 IMGNANDD147 pKa = 4.04 SYY149 pKa = 11.43 TPYY152 pKa = 10.69 LYY154 pKa = 10.76 AFNPILSPVTCGSKK168 pKa = 8.47 MQLYY172 pKa = 9.64 AALYY176 pKa = 10.39 KK177 pKa = 10.13 KK178 pKa = 10.69 FKK180 pKa = 10.4 YY181 pKa = 10.55 VGIKK185 pKa = 10.23 VSWHH189 pKa = 6.36 PLAKK193 pKa = 9.04 PTTTVVSTSMTVNPGMHH210 pKa = 7.24 DD211 pKa = 3.4 EE212 pKa = 4.71 TYY214 pKa = 10.02 QAYY217 pKa = 10.72 SIDD220 pKa = 3.51 AGKK223 pKa = 10.63 KK224 pKa = 8.66 VDD226 pKa = 3.35 SFMNSGTTQGLYY238 pKa = 10.41 LKK240 pKa = 9.67 HH241 pKa = 7.04 NSMEE245 pKa = 4.29 RR246 pKa = 11.84 NVRR249 pKa = 11.84 AKK251 pKa = 10.81 DD252 pKa = 4.31 FIPMSTQTLYY262 pKa = 9.1 MHH264 pKa = 6.96 VYY266 pKa = 9.62 FGKK269 pKa = 10.48 QIEE272 pKa = 4.7 SSQNWHH278 pKa = 6.65 LPFINKK284 pKa = 9.09 VSEE287 pKa = 4.2 TGGVTGYY294 pKa = 11.17 AVAHH298 pKa = 6.95 GINDD302 pKa = 3.57 NQRR305 pKa = 11.84 GRR307 pKa = 11.84 LKK309 pKa = 10.56 AAPFYY314 pKa = 10.4 DD315 pKa = 3.81 IKK317 pKa = 10.61 TMMPCIEE324 pKa = 4.87 RR325 pKa = 11.84 DD326 pKa = 3.6 SKK328 pKa = 10.51 LHH330 pKa = 6.04 KK331 pKa = 10.49 VYY333 pKa = 11.14 DD334 pKa = 3.55 MSKK337 pKa = 8.64 PFSFFVRR344 pKa = 11.84 PMVSSNEE351 pKa = 3.85 VSKK354 pKa = 10.86 EE355 pKa = 3.46 AGINVTQEE363 pKa = 3.65 AGEE366 pKa = 4.83 LLTQPTNEE374 pKa = 4.02 IEE376 pKa = 4.05 QLQKK380 pKa = 10.97 GLKK383 pKa = 8.77 HH384 pKa = 6.32 LGYY387 pKa = 10.55 KK388 pKa = 10.2 EE389 pKa = 4.13 FTHH392 pKa = 6.75 SLTSNKK398 pKa = 9.84 LDD400 pKa = 3.51 IPQDD404 pKa = 3.43 AYY406 pKa = 11.18 SAQHH410 pKa = 6.1 AHH412 pKa = 7.07 DD413 pKa = 4.72 PQDD416 pKa = 3.1 KK417 pKa = 10.24 FYY419 pKa = 10.78 HH420 pKa = 5.92 RR421 pKa = 11.84 WSALSDD427 pKa = 3.46 DD428 pKa = 5.38 DD429 pKa = 5.66 NFFNPVMFWYY439 pKa = 10.57 CFTTSDD445 pKa = 4.33 RR446 pKa = 11.84 YY447 pKa = 9.63 VPTYY451 pKa = 10.66 AEE453 pKa = 3.96 YY454 pKa = 9.66 MKK456 pKa = 10.84 INDD459 pKa = 3.48 PHH461 pKa = 7.7 QLFNTNRR468 pKa = 11.84 FFTTTGEE475 pKa = 4.4 SNNAKK480 pKa = 10.16 YY481 pKa = 10.04 EE482 pKa = 3.86 LSARR486 pKa = 11.84 IDD488 pKa = 3.35 TSAPFLNGYY497 pKa = 10.22 GYY499 pKa = 11.11 FDD501 pKa = 3.18 VTFYY505 pKa = 10.86 TKK507 pKa = 9.77 WKK509 pKa = 9.49 EE510 pKa = 3.75 EE511 pKa = 3.93 RR512 pKa = 11.84 PLVSKK517 pKa = 9.58 TSYY520 pKa = 10.59 VGIVPSEE527 pKa = 4.08 TGVEE531 pKa = 4.47 GVSSIIGNN539 pKa = 3.69
Molecular weight: 61.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.803
IPC2_protein 9.341
IPC_protein 9.253
Toseland 9.692
ProMoST 9.531
Dawson 9.999
Bjellqvist 9.721
Wikipedia 10.204
Rodwell 10.277
Grimsley 10.101
Solomon 10.014
Lehninger 9.955
Nozaki 9.706
DTASelect 9.706
Thurlkill 9.823
EMBOSS 10.131
Sillero 9.911
Patrickios 4.749
IPC_peptide 9.999
IPC2_peptide 8.317
IPC2.peptide.svr19 8.222
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
889
350
539
444.5
51.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.512 ± 0.728
0.675 ± 0.108
4.612 ± 0.485
6.524 ± 2.233
4.274 ± 0.841
6.074 ± 0.126
2.025 ± 0.354
5.737 ± 1.004
8.661 ± 0.116
7.424 ± 2.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.262 ± 0.24
5.512 ± 0.219
5.287 ± 0.085
3.825 ± 0.443
5.174 ± 0.528
6.299 ± 2.213
6.412 ± 0.584
4.949 ± 0.055
1.687 ± 0.525
6.074 ± 1.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here