Tsukamurella phage TPA4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A159B6U2|A0A159B6U2_9CAUD Uncharacterized protein OS=Tsukamurella phage TPA4 OX=1647476 GN=TPA4_80 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 6.22DD3 pKa = 5.5DD4 pKa = 5.93DD5 pKa = 4.16WAQLADD11 pKa = 4.93LGDD14 pKa = 4.36CGALLDD20 pKa = 4.73LPPLALCPLIAEE32 pKa = 4.72LAWLAAQKK40 pKa = 10.7SIDD43 pKa = 4.32VIAGLCGADD52 pKa = 3.55PVSLDD57 pKa = 4.0FIRR60 pKa = 11.84PP61 pKa = 3.52

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A159B6Q0|A0A159B6Q0_9CAUD Uncharacterized protein OS=Tsukamurella phage TPA4 OX=1647476 GN=TPA4_36 PE=4 SV=1
MM1 pKa = 7.1STPITVATVRR11 pKa = 11.84GCGEE15 pKa = 4.18PLTNHH20 pKa = 7.11LLLEE24 pKa = 4.75VIRR27 pKa = 11.84QLPKK31 pKa = 10.29DD32 pKa = 3.48RR33 pKa = 11.84FRR35 pKa = 11.84HH36 pKa = 4.56VEE38 pKa = 3.82IVHH41 pKa = 6.65PATMGPVGGPINSPSMLATINEE63 pKa = 4.4GVRR66 pKa = 11.84LLDD69 pKa = 3.49GAYY72 pKa = 9.73AAARR76 pKa = 11.84GRR78 pKa = 11.84FVGLGYY84 pKa = 10.37SQGGSLLTEE93 pKa = 4.08WLNRR97 pKa = 11.84RR98 pKa = 11.84DD99 pKa = 3.58PGGQGGGLPKK109 pKa = 10.35LALAGFIANPSRR121 pKa = 11.84VEE123 pKa = 3.76GRR125 pKa = 11.84SYY127 pKa = 11.05GRR129 pKa = 11.84KK130 pKa = 9.63APGQGIYY137 pKa = 10.29AANQRR142 pKa = 11.84HH143 pKa = 5.19RR144 pKa = 11.84LGWAEE149 pKa = 3.58IAAPGDD155 pKa = 3.76VITAMSADD163 pKa = 3.48SPLRR167 pKa = 11.84TGAGLIRR174 pKa = 11.84AMSLADD180 pKa = 4.28PIGWALDD187 pKa = 3.44VAPQLTAARR196 pKa = 11.84LAILGRR202 pKa = 11.84EE203 pKa = 4.07PWQAVNPRR211 pKa = 11.84FWTRR215 pKa = 11.84WKK217 pKa = 10.33EE218 pKa = 4.27AIDD221 pKa = 3.71GMYY224 pKa = 10.27AYY226 pKa = 10.31ARR228 pKa = 11.84LGSHH232 pKa = 5.77TDD234 pKa = 2.65AYY236 pKa = 10.62RR237 pKa = 11.84RR238 pKa = 11.84RR239 pKa = 11.84DD240 pKa = 3.2WKK242 pKa = 10.55DD243 pKa = 2.62WRR245 pKa = 11.84GQPVSGIDD253 pKa = 3.3LLARR257 pKa = 11.84AVKK260 pKa = 7.89TTAAA264 pKa = 3.96

Molecular weight: 28.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

17568

38

1719

209.1

22.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.716 ± 0.555

0.945 ± 0.144

6.654 ± 0.304

5.459 ± 0.309

2.47 ± 0.157

9.477 ± 0.449

2.117 ± 0.193

3.967 ± 0.172

2.852 ± 0.273

8.043 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.116

2.607 ± 0.204

6.278 ± 0.407

3.381 ± 0.206

7.508 ± 0.401

4.958 ± 0.354

7.246 ± 0.26

7.314 ± 0.32

1.873 ± 0.171

2.038 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski