Shinobi tetravirus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses

Average proteome isoelectric point is 8.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A347ZKJ0|A0A347ZKJ0_9VIRU Hypothetical protein protein OS=Shinobi tetravirus OX=2006394 GN=hypothetical protein PE=4 SV=1
MM1 pKa = 6.94MNSVRR6 pKa = 11.84GFVTVNRR13 pKa = 11.84AFGNAAACDD22 pKa = 3.79LLLAEE27 pKa = 4.99GALTAFSHH35 pKa = 5.84EE36 pKa = 4.83EE37 pKa = 3.5IMSARR42 pKa = 11.84KK43 pKa = 9.63KK44 pKa = 10.21RR45 pKa = 11.84VYY47 pKa = 10.42CAGTHH52 pKa = 4.24KK53 pKa = 9.87TFVEE57 pKa = 4.38RR58 pKa = 11.84TKK60 pKa = 11.15LLLGRR65 pKa = 11.84KK66 pKa = 8.89CKK68 pKa = 10.49SPYY71 pKa = 9.91KK72 pKa = 8.93YY73 pKa = 9.1TVVAQKK79 pKa = 10.01LQKK82 pKa = 10.18FMPVGVLPDD91 pKa = 3.35WTDD94 pKa = 2.8VDD96 pKa = 4.2SLLEE100 pKa = 3.98RR101 pKa = 11.84VKK103 pKa = 9.79VTKK106 pKa = 10.41AAGAGAPYY114 pKa = 10.06WKK116 pKa = 10.33DD117 pKa = 2.88KK118 pKa = 11.2GDD120 pKa = 4.14AFDD123 pKa = 4.46EE124 pKa = 4.42CMQEE128 pKa = 4.38ILPTLIKK135 pKa = 10.44AINDD139 pKa = 3.57GTVEE143 pKa = 3.77QLARR147 pKa = 11.84GRR149 pKa = 11.84PEE151 pKa = 3.5WFLVEE156 pKa = 4.68LKK158 pKa = 11.04NKK160 pKa = 8.97IDD162 pKa = 3.81RR163 pKa = 11.84YY164 pKa = 9.58EE165 pKa = 4.02LPISKK170 pKa = 8.21TRR172 pKa = 11.84PYY174 pKa = 11.24GCFPFHH180 pKa = 5.94WTLLFSCLIQPFCEE194 pKa = 4.36ALSVWTDD201 pKa = 2.89SKK203 pKa = 10.2TCNAYY208 pKa = 10.16GMSFCSGNLKK218 pKa = 9.98KK219 pKa = 9.96WFSRR223 pKa = 11.84VEE225 pKa = 4.01SLIATAKK232 pKa = 10.34KK233 pKa = 10.04KK234 pKa = 10.72NKK236 pKa = 9.42DD237 pKa = 3.26MYY239 pKa = 10.87MMGVYY244 pKa = 10.57GDD246 pKa = 4.18DD247 pKa = 3.98CRR249 pKa = 11.84LIQAKK254 pKa = 10.2ASGGAVVEE262 pKa = 4.48DD263 pKa = 4.73PDD265 pKa = 3.95MKK267 pKa = 10.93QHH269 pKa = 7.16DD270 pKa = 4.25GAIDD274 pKa = 3.54TEE276 pKa = 4.85TISGTVKK283 pKa = 10.33WIYY286 pKa = 11.43DD287 pKa = 3.71MYY289 pKa = 11.13AKK291 pKa = 10.06QHH293 pKa = 6.2GDD295 pKa = 3.17SKK297 pKa = 10.7FWEE300 pKa = 4.75FVLQQLAAFACDD312 pKa = 3.51PNMVIDD318 pKa = 4.92GPTVYY323 pKa = 9.56TKK325 pKa = 10.39KK326 pKa = 10.79VPGGLISGVPGTTLFGCAKK345 pKa = 10.21SVLAYY350 pKa = 10.35AGLVDD355 pKa = 3.89EE356 pKa = 5.6CMFDD360 pKa = 2.94KK361 pKa = 10.95TLFLNEE367 pKa = 4.43AKK369 pKa = 10.6VKK371 pKa = 10.19AYY373 pKa = 10.84LMEE376 pKa = 4.09KK377 pKa = 9.82HH378 pKa = 6.11GCALKK383 pKa = 10.9DD384 pKa = 3.7GTWRR388 pKa = 11.84PSPMNLSQADD398 pKa = 3.72GTYY401 pKa = 10.11LCEE404 pKa = 4.45SKK406 pKa = 10.8FLGMRR411 pKa = 11.84LMWRR415 pKa = 11.84TSEE418 pKa = 4.0QFPEE422 pKa = 4.39PFLVPTLHH430 pKa = 7.03DD431 pKa = 4.41GEE433 pKa = 4.22WLEE436 pKa = 6.24LIMCPRR442 pKa = 11.84TSGDD446 pKa = 2.98KK447 pKa = 10.59KK448 pKa = 10.72GSHH451 pKa = 6.63LSEE454 pKa = 3.73QRR456 pKa = 11.84TSFDD460 pKa = 3.92RR461 pKa = 11.84IRR463 pKa = 11.84GLLLCGAVYY472 pKa = 10.03SEE474 pKa = 4.46RR475 pKa = 11.84ARR477 pKa = 11.84AALLAAVDD485 pKa = 4.64LVPATAVLLSTQLDD499 pKa = 3.74GRR501 pKa = 11.84KK502 pKa = 9.85GLIEE506 pKa = 4.42SPNDD510 pKa = 3.44LVVSEE515 pKa = 4.63DD516 pKa = 4.13FIYY519 pKa = 10.25PSTAGIPGLEE529 pKa = 4.3FCDD532 pKa = 3.58ALYY535 pKa = 11.01NLSAEE540 pKa = 4.09QRR542 pKa = 11.84EE543 pKa = 4.7GCKK546 pKa = 10.2FEE548 pKa = 4.87SVYY551 pKa = 9.29PTLDD555 pKa = 3.29DD556 pKa = 3.93FVNSRR561 pKa = 11.84KK562 pKa = 9.14EE563 pKa = 3.66LHH565 pKa = 6.53RR566 pKa = 11.84SRR568 pKa = 11.84FRR570 pKa = 11.84YY571 pKa = 9.55IIHH574 pKa = 6.33QSLPAKK580 pKa = 8.43PTAKK584 pKa = 10.1PIPAPRR590 pKa = 11.84SYY592 pKa = 8.43THH594 pKa = 6.68KK595 pKa = 10.0HH596 pKa = 4.92TGQQVFLPKK605 pKa = 9.85PAPRR609 pKa = 11.84LQTTSEE615 pKa = 4.0QPAMDD620 pKa = 4.51KK621 pKa = 10.41EE622 pKa = 4.53VQEE625 pKa = 4.14QEE627 pKa = 4.36PPLFTANEE635 pKa = 4.35PIVAVEE641 pKa = 4.3EE642 pKa = 4.44EE643 pKa = 4.52FTSEE647 pKa = 4.09DD648 pKa = 3.67LEE650 pKa = 4.89KK651 pKa = 10.6IPLPPIPEE659 pKa = 4.37PQHH662 pKa = 5.65GFARR666 pKa = 11.84GGRR669 pKa = 11.84EE670 pKa = 3.66VRR672 pKa = 11.84AGSLHH677 pKa = 6.52SKK679 pKa = 10.0DD680 pKa = 3.93GKK682 pKa = 9.37TIAPRR687 pKa = 11.84HH688 pKa = 5.13KK689 pKa = 9.87LQRR692 pKa = 11.84LEE694 pKa = 4.22TYY696 pKa = 10.35LGEE699 pKa = 4.31GLKK702 pKa = 10.69KK703 pKa = 9.85NAKK706 pKa = 9.32ALVMSNPVLPEE717 pKa = 3.75GTVRR721 pKa = 11.84NYY723 pKa = 10.28QCLFSAVLDD732 pKa = 4.41RR733 pKa = 11.84GFKK736 pKa = 9.31WPHH739 pKa = 6.53DD740 pKa = 4.25LPEE743 pKa = 4.53GTHH746 pKa = 7.1DD747 pKa = 3.75NALVGQFAAINGLKK761 pKa = 10.2SSPEE765 pKa = 3.78TSVIDD770 pKa = 3.78PAQDD774 pKa = 3.29MKK776 pKa = 10.76GVWMRR781 pKa = 11.84LVPKK785 pKa = 10.28DD786 pKa = 3.74GLGSSIDD793 pKa = 3.9YY794 pKa = 10.54AQLTATGCTLKK805 pKa = 10.73RR806 pKa = 11.84MQAAINQQFAAWLRR820 pKa = 11.84EE821 pKa = 3.92FKK823 pKa = 10.44EE824 pKa = 4.59SFMEE828 pKa = 4.27PPKK831 pKa = 10.68KK832 pKa = 10.35VIKK835 pKa = 9.42PPPGAPGFTSSNWAAEE851 pKa = 4.18VEE853 pKa = 4.21SDD855 pKa = 3.64DD856 pKa = 4.17LSRR859 pKa = 11.84MGSLSEE865 pKa = 4.28ASLKK869 pKa = 9.97EE870 pKa = 3.91VQRR873 pKa = 11.84VVRR876 pKa = 11.84DD877 pKa = 3.34LLRR880 pKa = 11.84TEE882 pKa = 4.52FAYY885 pKa = 10.79LRR887 pKa = 11.84TLDD890 pKa = 3.87RR891 pKa = 11.84EE892 pKa = 4.25HH893 pKa = 6.88TSRR896 pKa = 11.84HH897 pKa = 4.47GTKK900 pKa = 9.32QQHH903 pKa = 5.91KK904 pKa = 8.75VSKK907 pKa = 10.65SPASPTNAEE916 pKa = 3.85EE917 pKa = 4.2EE918 pKa = 4.53GRR920 pKa = 11.84SEE922 pKa = 4.28GRR924 pKa = 11.84DD925 pKa = 3.3ISPVEE930 pKa = 3.97ASPSRR935 pKa = 11.84TVPSAGDD942 pKa = 3.26NN943 pKa = 3.53

Molecular weight: 104.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A347ZKJ1|A0A347ZKJ1_9VIRU Putative capsid protein protein OS=Shinobi tetravirus OX=2006394 GN=putative capsid protein PE=4 SV=1
MM1 pKa = 7.59ARR3 pKa = 11.84NNNIKK8 pKa = 10.3SASRR12 pKa = 11.84PQAQPTRR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.61RR22 pKa = 11.84GGVKK26 pKa = 10.27DD27 pKa = 3.49VTSRR31 pKa = 11.84QWKK34 pKa = 8.5QVQAGLSRR42 pKa = 11.84ALEE45 pKa = 4.38IIRR48 pKa = 11.84SPLCFVPLILSIFLFYY64 pKa = 10.59DD65 pKa = 3.95DD66 pKa = 6.01KK67 pKa = 11.76LLTQLANTFSANAAFKK83 pKa = 10.27WLGTFISEE91 pKa = 4.55HH92 pKa = 6.21KK93 pKa = 10.22KK94 pKa = 10.58QIAGLIFLVPATFLSCDD111 pKa = 4.03PKK113 pKa = 10.3WATLVSAVVSYY124 pKa = 10.44LVSEE128 pKa = 4.33VFPAPVKK135 pKa = 9.14YY136 pKa = 10.28FEE138 pKa = 4.09YY139 pKa = 10.71GFLGGSLILLCTARR153 pKa = 11.84KK154 pKa = 8.98PIQFVVATVVALLTIGLGLWGSEE177 pKa = 4.11VFASLPSTSRR187 pKa = 11.84QGG189 pKa = 3.11

Molecular weight: 20.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1627

189

943

542.3

59.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.498 ± 0.991

3.012 ± 0.838

4.364 ± 0.87

5.224 ± 1.296

4.364 ± 0.448

7.314 ± 0.388

2.274 ± 0.401

3.442 ± 0.436

5.778 ± 1.281

9.65 ± 0.537

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.028 ± 0.522

2.52 ± 0.038

7.376 ± 0.935

3.38 ± 0.384

5.286 ± 0.015

7.314 ± 0.393

7.253 ± 1.389

7.314 ± 0.978

1.782 ± 0.159

2.827 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski