Amino acid dipepetide frequency for Xylaria multiplex

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.795AlaAla: 8.795 ± 0.061	1.042AlaCys: 1.042 ± 0.015	4.324AlaAsp: 4.324 ± 0.027	4.936AlaGlu: 4.936 ± 0.037	3.135AlaPhe: 3.135 ± 0.027	5.673AlaGly: 5.673 ± 0.039	1.775AlaHis: 1.775 ± 0.018	4.541AlaIle: 4.541 ± 0.035	3.989AlaLys: 3.989 ± 0.03	7.798AlaLeu: 7.798 ± 0.048	1.817AlaMet: 1.817 ± 0.018	3.077AlaAsn: 3.077 ± 0.026	4.459AlaPro: 4.459 ± 0.038	3.276AlaGln: 3.276 ± 0.025	4.902AlaArg: 4.902 ± 0.035	7.073AlaSer: 7.073 ± 0.042	5.452AlaThr: 5.452 ± 0.036	5.358AlaVal: 5.358 ± 0.033	1.165AlaTrp: 1.165 ± 0.016	2.229AlaTyr: 2.229 ± 0.022	0.008AlaXaa: 0.008 ± 0.001
Cys	0.898CysAla: 0.898 ± 0.014	0.221CysCys: 0.221 ± 0.007	0.665CysAsp: 0.665 ± 0.011	0.612CysGlu: 0.612 ± 0.012	0.51CysPhe: 0.51 ± 0.009	0.878CysGly: 0.878 ± 0.016	0.316CysHis: 0.316 ± 0.007	0.699CysIle: 0.699 ± 0.011	0.482CysLys: 0.482 ± 0.009	1.198CysLeu: 1.198 ± 0.016	0.231CysMet: 0.231 ± 0.006	0.435CysAsn: 0.435 ± 0.008	0.638CysPro: 0.638 ± 0.012	0.433CysGln: 0.433 ± 0.008	0.725CysArg: 0.725 ± 0.013	0.869CysSer: 0.869 ± 0.015	0.647CysThr: 0.647 ± 0.011	0.766CysVal: 0.766 ± 0.012	0.197CysTrp: 0.197 ± 0.006	0.358CysTyr: 0.358 ± 0.008	0.002CysXaa: 0.002 ± 0.001
Asp	4.768AspAla: 4.768 ± 0.031	0.637AspCys: 0.637 ± 0.011	4.478AspAsp: 4.478 ± 0.048	4.574AspGlu: 4.574 ± 0.035	2.272AspPhe: 2.272 ± 0.02	4.226AspGly: 4.226 ± 0.027	1.24AspHis: 1.24 ± 0.015	3.399AspIle: 3.399 ± 0.022	2.407AspLys: 2.407 ± 0.022	5.126AspLeu: 5.126 ± 0.036	1.227AspMet: 1.227 ± 0.015	2.085AspAsn: 2.085 ± 0.018	3.339AspPro: 3.339 ± 0.025	1.858AspGln: 1.858 ± 0.021	3.002AspArg: 3.002 ± 0.029	4.259AspSer: 4.259 ± 0.033	3.106AspThr: 3.106 ± 0.022	3.714AspVal: 3.714 ± 0.028	0.93AspTrp: 0.93 ± 0.013	1.702AspTyr: 1.702 ± 0.018	0.004AspXaa: 0.004 ± 0.001
Glu	5.374GluAla: 5.374 ± 0.033	0.645GluCys: 0.645 ± 0.012	4.162GluAsp: 4.162 ± 0.035	4.988GluGlu: 4.988 ± 0.045	2.042GluPhe: 2.042 ± 0.02	3.648GluGly: 3.648 ± 0.024	1.376GluHis: 1.376 ± 0.018	3.239GluIle: 3.239 ± 0.026	3.534GluLys: 3.534 ± 0.03	5.37GluLeu: 5.37 ± 0.032	1.351GluMet: 1.351 ± 0.015	2.421GluAsn: 2.421 ± 0.022	2.829GluPro: 2.829 ± 0.027	2.341GluGln: 2.341 ± 0.025	3.872GluArg: 3.872 ± 0.035	4.338GluSer: 4.338 ± 0.031	3.518GluThr: 3.518 ± 0.029	3.475GluVal: 3.475 ± 0.028	0.906GluTrp: 0.906 ± 0.013	1.731GluTyr: 1.731 ± 0.019	0.005GluXaa: 0.005 ± 0.001
Phe	3.038PheAla: 3.038 ± 0.023	0.521PheCys: 0.521 ± 0.012	2.345PheAsp: 2.345 ± 0.019	2.214PheGlu: 2.214 ± 0.022	1.554PhePhe: 1.554 ± 0.017	2.853PheGly: 2.853 ± 0.029	0.901PheHis: 0.901 ± 0.012	1.895PheIle: 1.895 ± 0.022	1.555PheLys: 1.555 ± 0.016	3.441PheLeu: 3.441 ± 0.027	0.729PheMet: 0.729 ± 0.012	1.499PheAsn: 1.499 ± 0.017	1.966PhePro: 1.966 ± 0.02	1.391PheGln: 1.391 ± 0.014	2.003PheArg: 2.003 ± 0.02	2.97PheSer: 2.97 ± 0.021	2.198PheThr: 2.198 ± 0.02	2.385PheVal: 2.385 ± 0.023	0.66PheTrp: 0.66 ± 0.011	1.13PheTyr: 1.13 ± 0.014	0.013PheXaa: 0.013 ± 0.002
Gly	5.373GlyAla: 5.373 ± 0.042	0.827GlyCys: 0.827 ± 0.013	3.837GlyAsp: 3.837 ± 0.032	3.63GlyGlu: 3.63 ± 0.029	2.827GlyPhe: 2.827 ± 0.024	5.646GlyGly: 5.646 ± 0.042	1.727GlyHis: 1.727 ± 0.021	3.716GlyIle: 3.716 ± 0.031	3.337GlyLys: 3.337 ± 0.025	6.085GlyLeu: 6.085 ± 0.038	1.414GlyMet: 1.414 ± 0.015	2.731GlyAsn: 2.731 ± 0.025	3.36GlyPro: 3.36 ± 0.028	2.454GlyGln: 2.454 ± 0.023	4.106GlyArg: 4.106 ± 0.027	5.631GlySer: 5.631 ± 0.033	4.086GlyThr: 4.086 ± 0.028	4.385GlyVal: 4.385 ± 0.032	1.159GlyTrp: 1.159 ± 0.016	2.125GlyTyr: 2.125 ± 0.021	0.015GlyXaa: 0.015 ± 0.002
His	1.759HisAla: 1.759 ± 0.018	0.324HisCys: 0.324 ± 0.009	1.39HisAsp: 1.39 ± 0.016	1.359HisGlu: 1.359 ± 0.016	0.92HisPhe: 0.92 ± 0.012	1.74HisGly: 1.74 ± 0.019	0.856HisHis: 0.856 ± 0.015	1.328HisIle: 1.328 ± 0.016	0.918HisLys: 0.918 ± 0.014	2.189HisLeu: 2.189 ± 0.02	0.486HisMet: 0.486 ± 0.009	0.92HisAsn: 0.92 ± 0.015	1.592HisPro: 1.592 ± 0.018	0.988HisGln: 0.988 ± 0.014	1.507HisArg: 1.507 ± 0.016	1.802HisSer: 1.802 ± 0.02	1.279HisThr: 1.279 ± 0.017	1.393HisVal: 1.393 ± 0.016	0.342HisTrp: 0.342 ± 0.009	0.728HisTyr: 0.728 ± 0.011	0.002HisXaa: 0.002 ± 0.001
Ile	4.388IleAla: 4.388 ± 0.03	0.721IleCys: 0.721 ± 0.012	3.192IleAsp: 3.192 ± 0.024	3.133IleGlu: 3.133 ± 0.023	2.082IlePhe: 2.082 ± 0.021	3.394IleGly: 3.394 ± 0.028	1.25IleHis: 1.25 ± 0.015	2.82IleIle: 2.82 ± 0.025	2.305IleLys: 2.305 ± 0.023	4.742IleLeu: 4.742 ± 0.036	1.038IleMet: 1.038 ± 0.013	2.009IleAsn: 2.009 ± 0.022	3.258IlePro: 3.258 ± 0.025	2.018IleGln: 2.018 ± 0.02	2.978IleArg: 2.978 ± 0.025	4.092IleSer: 4.092 ± 0.026	3.129IleThr: 3.129 ± 0.023	3.382IleVal: 3.382 ± 0.025	0.789IleTrp: 0.789 ± 0.013	1.469IleTyr: 1.469 ± 0.017	0.005IleXaa: 0.005 ± 0.001
Lys	4.204LysAla: 4.204 ± 0.033	0.466LysCys: 0.466 ± 0.01	2.726LysAsp: 2.726 ± 0.024	3.129LysGlu: 3.129 ± 0.027	1.49LysPhe: 1.49 ± 0.018	2.937LysGly: 2.937 ± 0.026	1.083LysHis: 1.083 ± 0.012	2.347LysIle: 2.347 ± 0.021	3.052LysLys: 3.052 ± 0.04	4.172LysLeu: 4.172 ± 0.027	0.946LysMet: 0.946 ± 0.013	1.756LysAsn: 1.756 ± 0.019	2.701LysPro: 2.701 ± 0.028	1.792LysGln: 1.792 ± 0.018	3.309LysArg: 3.309 ± 0.023	3.418LysSer: 3.418 ± 0.025	2.806LysThr: 2.806 ± 0.024	2.714LysVal: 2.714 ± 0.024	0.685LysTrp: 0.685 ± 0.01	1.409LysTyr: 1.409 ± 0.017	0.022LysXaa: 0.022 ± 0.002
Leu	7.91LeuAla: 7.91 ± 0.04	1.174LeuCys: 1.174 ± 0.016	5.308LeuAsp: 5.308 ± 0.031	5.613LeuGlu: 5.613 ± 0.038	3.278LeuPhe: 3.278 ± 0.027	6.084LeuGly: 6.084 ± 0.041	2.213LeuHis: 2.213 ± 0.018	4.164LeuIle: 4.164 ± 0.032	4.18LeuLys: 4.18 ± 0.03	8.407LeuLeu: 8.407 ± 0.055	1.676LeuMet: 1.676 ± 0.017	3.255LeuAsn: 3.255 ± 0.025	5.42LeuPro: 5.42 ± 0.035	3.728LeuGln: 3.728 ± 0.031	5.813LeuArg: 5.813 ± 0.034	7.322LeuSer: 7.322 ± 0.037	4.856LeuThr: 4.856 ± 0.029	5.556LeuVal: 5.556 ± 0.038	1.261LeuTrp: 1.261 ± 0.016	2.382LeuTyr: 2.382 ± 0.023	0.005LeuXaa: 0.005 ± 0.001
Met	2.078MetAla: 2.078 ± 0.021	0.217MetCys: 0.217 ± 0.006	1.155MetAsp: 1.155 ± 0.015	1.17MetGlu: 1.17 ± 0.014	0.688MetPhe: 0.688 ± 0.011	1.345MetGly: 1.345 ± 0.015	0.45MetHis: 0.45 ± 0.009	0.982MetIle: 0.982 ± 0.015	0.972MetLys: 0.972 ± 0.014	1.714MetLeu: 1.714 ± 0.017	0.544MetMet: 0.544 ± 0.01	0.769MetAsn: 0.769 ± 0.013	1.199MetPro: 1.199 ± 0.016	0.773MetGln: 0.773 ± 0.012	1.271MetArg: 1.271 ± 0.015	1.726MetSer: 1.726 ± 0.018	1.258MetThr: 1.258 ± 0.016	1.185MetVal: 1.185 ± 0.014	0.243MetTrp: 0.243 ± 0.007	0.477MetTyr: 0.477 ± 0.008	0.001MetXaa: 0.001 ± 0.0
Asn	3.185AsnAla: 3.185 ± 0.024	0.453AsnCys: 0.453 ± 0.009	2.13AsnAsp: 2.13 ± 0.023	2.151AsnGlu: 2.151 ± 0.02	1.442AsnPhe: 1.442 ± 0.016	3.081AsnGly: 3.081 ± 0.031	0.887AsnHis: 0.887 ± 0.011	2.277AsnIle: 2.277 ± 0.022	1.604AsnLys: 1.604 ± 0.018	3.36AsnLeu: 3.36 ± 0.025	0.84AsnMet: 0.84 ± 0.012	1.658AsnAsn: 1.658 ± 0.024	2.546AsnPro: 2.546 ± 0.024	1.388AsnGln: 1.388 ± 0.015	2.022AsnArg: 2.022 ± 0.021	2.962AsnSer: 2.962 ± 0.025	2.436AsnThr: 2.436 ± 0.022	2.313AsnVal: 2.313 ± 0.02	0.592AsnTrp: 0.592 ± 0.011	1.135AsnTyr: 1.135 ± 0.014	0.003AsnXaa: 0.003 ± 0.001
Pro	4.716ProAla: 4.716 ± 0.038	0.478ProCys: 0.478 ± 0.011	3.272ProAsp: 3.272 ± 0.024	3.817ProGlu: 3.817 ± 0.032	2.066ProPhe: 2.066 ± 0.021	3.927ProGly: 3.927 ± 0.031	1.325ProHis: 1.325 ± 0.017	2.825ProIle: 2.825 ± 0.023	2.605ProLys: 2.605 ± 0.027	4.761ProLeu: 4.761 ± 0.031	1.011ProMet: 1.011 ± 0.015	2.383ProAsn: 2.383 ± 0.02	4.894ProPro: 4.894 ± 0.061	2.437ProGln: 2.437 ± 0.025	3.469ProArg: 3.469 ± 0.03	5.872ProSer: 5.872 ± 0.048	4.139ProThr: 4.139 ± 0.036	3.444ProVal: 3.444 ± 0.028	0.784ProTrp: 0.784 ± 0.012	1.555ProTyr: 1.555 ± 0.017	0.019ProXaa: 0.019 ± 0.002
Gln	3.331GlnAla: 3.331 ± 0.03	0.428GlnCys: 0.428 ± 0.01	2.121GlnAsp: 2.121 ± 0.019	2.314GlnGlu: 2.314 ± 0.023	1.309GlnPhe: 1.309 ± 0.015	2.409GlnGly: 2.409 ± 0.021	1.039GlnHis: 1.039 ± 0.015	1.943GlnIle: 1.943 ± 0.019	1.887GlnLys: 1.887 ± 0.019	3.488GlnLeu: 3.488 ± 0.028	0.805GlnMet: 0.805 ± 0.01	1.555GlnAsn: 1.555 ± 0.019	2.504GlnPro: 2.504 ± 0.025	2.261GlnGln: 2.261 ± 0.043	2.518GlnArg: 2.518 ± 0.02	3.032GlnSer: 3.032 ± 0.028	2.29GlnThr: 2.29 ± 0.021	2.162GlnVal: 2.162 ± 0.019	0.612GlnTrp: 0.612 ± 0.011	1.194GlnTyr: 1.194 ± 0.014	0.004GlnXaa: 0.004 ± 0.001
Arg	4.7ArgAla: 4.7 ± 0.033	0.687ArgCys: 0.687 ± 0.012	3.585ArgAsp: 3.585 ± 0.029	3.852ArgGlu: 3.852 ± 0.032	2.165ArgPhe: 2.165 ± 0.02	3.803ArgGly: 3.803 ± 0.03	1.599ArgHis: 1.599 ± 0.017	3.042ArgIle: 3.042 ± 0.025	3.276ArgLys: 3.276 ± 0.023	5.541ArgLeu: 5.541 ± 0.031	1.238ArgMet: 1.238 ± 0.015	2.354ArgAsn: 2.354 ± 0.017	3.501ArgPro: 3.501 ± 0.03	2.611ArgGln: 2.611 ± 0.022	5.008ArgArg: 5.008 ± 0.044	4.562ArgSer: 4.562 ± 0.037	3.202ArgThr: 3.202 ± 0.023	3.47ArgVal: 3.47 ± 0.024	0.948ArgTrp: 0.948 ± 0.013	1.706ArgTyr: 1.706 ± 0.017	0.008ArgXaa: 0.008 ± 0.001
Ser	6.34SerAla: 6.34 ± 0.036	0.828SerCys: 0.828 ± 0.012	4.222SerAsp: 4.222 ± 0.031	4.112SerGlu: 4.112 ± 0.031	3.028SerPhe: 3.028 ± 0.022	5.496SerGly: 5.496 ± 0.036	1.978SerHis: 1.978 ± 0.019	4.25SerIle: 4.25 ± 0.026	3.641SerLys: 3.641 ± 0.033	7.242SerLeu: 7.242 ± 0.043	1.577SerMet: 1.577 ± 0.018	3.147SerAsn: 3.147 ± 0.023	5.381SerPro: 5.381 ± 0.05	3.315SerGln: 3.315 ± 0.029	5.058SerArg: 5.058 ± 0.04	8.56SerSer: 8.56 ± 0.067	5.648SerThr: 5.648 ± 0.041	4.494SerVal: 4.494 ± 0.03	1.19SerTrp: 1.19 ± 0.016	2.159SerTyr: 2.159 ± 0.024	0.008SerXaa: 0.008 ± 0.001
Thr	5.247ThrAla: 5.247 ± 0.034	0.734ThrCys: 0.734 ± 0.012	2.925ThrAsp: 2.925 ± 0.022	3.201ThrGlu: 3.201 ± 0.026	2.252ThrPhe: 2.252 ± 0.02	4.168ThrGly: 4.168 ± 0.027	1.329ThrHis: 1.329 ± 0.016	3.365ThrIle: 3.365 ± 0.028	2.657ThrLys: 2.657 ± 0.025	5.306ThrLeu: 5.306 ± 0.035	1.17ThrMet: 1.17 ± 0.014	2.289ThrAsn: 2.289 ± 0.021	4.418ThrPro: 4.418 ± 0.035	2.194ThrGln: 2.194 ± 0.022	3.271ThrArg: 3.271 ± 0.027	5.408ThrSer: 5.408 ± 0.039	4.546ThrThr: 4.546 ± 0.039	3.702ThrVal: 3.702 ± 0.03	0.897ThrTrp: 0.897 ± 0.013	1.712ThrTyr: 1.712 ± 0.016	0.005ThrXaa: 0.005 ± 0.001
Val	5.307ValAla: 5.307 ± 0.031	0.764ValCys: 0.764 ± 0.011	3.816ValAsp: 3.816 ± 0.027	3.839ValGlu: 3.839 ± 0.028	2.424ValPhe: 2.424 ± 0.023	4.028ValGly: 4.028 ± 0.031	1.34ValHis: 1.34 ± 0.014	3.113ValIle: 3.113 ± 0.025	2.814ValLys: 2.814 ± 0.025	5.578ValLeu: 5.578 ± 0.033	1.195ValMet: 1.195 ± 0.015	2.309ValAsn: 2.309 ± 0.025	3.538ValPro: 3.538 ± 0.026	2.236ValGln: 2.236 ± 0.021	3.371ValArg: 3.371 ± 0.023	4.629ValSer: 4.629 ± 0.026	3.643ValThr: 3.643 ± 0.027	4.352ValVal: 4.352 ± 0.036	0.849ValTrp: 0.849 ± 0.013	1.738ValTyr: 1.738 ± 0.019	0.006ValXaa: 0.006 ± 0.001
Trp	1.218TrpAla: 1.218 ± 0.017	0.196TrpCys: 0.196 ± 0.006	0.962TrpAsp: 0.962 ± 0.015	0.886TrpGlu: 0.886 ± 0.012	0.564TrpPhe: 0.564 ± 0.009	0.978TrpGly: 0.978 ± 0.013	0.37TrpHis: 0.37 ± 0.008	0.772TrpIle: 0.772 ± 0.012	0.795TrpLys: 0.795 ± 0.011	1.376TrpLeu: 1.376 ± 0.018	0.332TrpMet: 0.332 ± 0.007	0.656TrpAsn: 0.656 ± 0.011	0.653TrpPro: 0.653 ± 0.011	0.584TrpGln: 0.584 ± 0.01	0.979TrpArg: 0.979 ± 0.014	1.051TrpSer: 1.051 ± 0.015	0.893TrpThr: 0.893 ± 0.013	0.956TrpVal: 0.956 ± 0.014	0.272TrpTrp: 0.272 ± 0.007	0.445TrpTyr: 0.445 ± 0.008	0.001TrpXaa: 0.001 ± 0.0
Tyr	2.185TyrAla: 2.185 ± 0.022	0.415TyrCys: 0.415 ± 0.01	1.737TyrAsp: 1.737 ± 0.018	1.629TyrGlu: 1.629 ± 0.018	1.204TyrPhe: 1.204 ± 0.014	2.136TyrGly: 2.136 ± 0.023	0.769TyrHis: 0.769 ± 0.012	1.476TyrIle: 1.476 ± 0.016	1.132TyrLys: 1.132 ± 0.015	2.705TyrLeu: 2.705 ± 0.025	0.587TyrMet: 0.587 ± 0.011	1.154TyrAsn: 1.154 ± 0.014	1.585TyrPro: 1.585 ± 0.022	1.125TyrGln: 1.125 ± 0.016	1.663TyrArg: 1.663 ± 0.018	2.089TyrSer: 2.089 ± 0.019	1.658TyrThr: 1.658 ± 0.016	1.682TyrVal: 1.682 ± 0.018	0.455TyrTrp: 0.455 ± 0.008	0.99TyrTyr: 0.99 ± 0.015	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.011XaaAla: 0.011 ± 0.001	0.002XaaCys: 0.002 ± 0.001	0.003XaaAsp: 0.003 ± 0.001	0.005XaaGlu: 0.005 ± 0.001	0.01XaaPhe: 0.01 ± 0.001	0.019XaaGly: 0.019 ± 0.002	0.002XaaHis: 0.002 ± 0.001	0.002XaaIle: 0.002 ± 0.0	0.022XaaLys: 0.022 ± 0.002	0.004XaaLeu: 0.004 ± 0.001	0.002XaaMet: 0.002 ± 0.001	0.004XaaAsn: 0.004 ± 0.001	0.019XaaPro: 0.019 ± 0.002	0.002XaaGln: 0.002 ± 0.001	0.01XaaArg: 0.01 ± 0.001	0.008XaaSer: 0.008 ± 0.001	0.004XaaThr: 0.004 ± 0.001	0.004XaaVal: 0.004 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.003XaaTyr: 0.003 ± 0.001	0.001XaaXaa: 0.001 ± 0.0

Statistics based on 10963 proteins (5903969 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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