Escherichia phage TL-2011c
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9L6H5|G9L6H5_9CAUD Uncharacterized protein OS=Escherichia phage TL-2011c OX=1124655 PE=4 SV=1
MM1 pKa = 7.62 GEE3 pKa = 3.93 VFMDD7 pKa = 4.26 DD8 pKa = 4.1 KK9 pKa = 11.01 IIAYY13 pKa = 8.38 FVVLSDD19 pKa = 3.63 EE20 pKa = 4.99 AISVIDD26 pKa = 3.53 TEE28 pKa = 4.75 GNVMFIAEE36 pKa = 4.49 HH37 pKa = 6.78 PEE39 pKa = 4.56 DD40 pKa = 3.76 IALQAAAYY48 pKa = 8.68 FFAKK52 pKa = 9.82 EE53 pKa = 3.91 QEE55 pKa = 4.37 EE56 pKa = 4.59 EE57 pKa = 4.71 CNCPVCQLSRR67 pKa = 11.84 QINLMHH73 pKa = 7.14
Molecular weight: 8.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.847
IPC2_protein 4.088
IPC_protein 3.948
Toseland 3.783
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.808
Rodwell 3.795
Grimsley 3.706
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.151
Thurlkill 3.821
EMBOSS 3.821
Sillero 4.062
Patrickios 0.769
IPC_peptide 3.897
IPC2_peptide 4.05
IPC2.peptide.svr19 3.974
Protein with the highest isoelectric point:
>tr|G9L6G1|G9L6G1_9CAUD Putative antirepressor OS=Escherichia phage TL-2011c OX=1124655 PE=4 SV=1
MM1 pKa = 7.11 KK2 pKa = 10.36 QIAFYY7 pKa = 10.4 RR8 pKa = 11.84 RR9 pKa = 11.84 SGRR12 pKa = 11.84 PGAFRR17 pKa = 11.84 GLKK20 pKa = 9.98 EE21 pKa = 3.78 RR22 pKa = 11.84 VTWMIQSRR30 pKa = 11.84 GRR32 pKa = 11.84 PVTGSEE38 pKa = 3.59 IAEE41 pKa = 4.12 KK42 pKa = 10.53 FGVSLCEE49 pKa = 3.92 FNKK52 pKa = 9.89 VARR55 pKa = 11.84 GLTKK59 pKa = 9.93 GSKK62 pKa = 8.32 VVKK65 pKa = 10.17 IKK67 pKa = 10.72 ASEE70 pKa = 4.1 PFTTDD75 pKa = 2.19 TGIVDD80 pKa = 3.88 RR81 pKa = 11.84 FFSLEE86 pKa = 4.02 SNPRR90 pKa = 11.84 RR91 pKa = 11.84 DD92 pKa = 3.57 TPRR95 pKa = 11.84 SRR97 pKa = 11.84 NAVPPFSRR105 pKa = 11.84 RR106 pKa = 11.84 SRR108 pKa = 11.84 EE109 pKa = 3.82 HH110 pKa = 5.97 AAKK113 pKa = 10.17 NCRR116 pKa = 11.84 EE117 pKa = 4.05 EE118 pKa = 4.13 YY119 pKa = 8.72 VQKK122 pKa = 10.9 AEE124 pKa = 3.91 RR125 pKa = 11.84 RR126 pKa = 11.84 RR127 pKa = 11.84 RR128 pKa = 11.84 LIKK131 pKa = 10.2 AGLYY135 pKa = 9.58 IDD137 pKa = 4.18 EE138 pKa = 5.21 FEE140 pKa = 4.32 NALL143 pKa = 3.75
Molecular weight: 16.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.706
IPC_protein 10.628
Toseland 10.95
ProMoST 10.774
Dawson 11.008
Bjellqvist 10.76
Wikipedia 11.257
Rodwell 11.169
Grimsley 11.038
Solomon 11.213
Lehninger 11.169
Nozaki 10.935
DTASelect 10.745
Thurlkill 10.935
EMBOSS 11.374
Sillero 10.95
Patrickios 10.906
IPC_peptide 11.213
IPC2_peptide 9.809
IPC2.peptide.svr19 8.718
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
17167
38
2263
238.4
26.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.53 ± 0.395
1.177 ± 0.237
5.732 ± 0.188
6.891 ± 0.268
3.588 ± 0.223
7.509 ± 0.519
1.782 ± 0.151
4.934 ± 0.303
5.674 ± 0.23
7.765 ± 0.236
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.72 ± 0.16
4.351 ± 0.171
4.474 ± 0.257
4.485 ± 0.352
6.606 ± 0.329
6.198 ± 0.232
5.895 ± 0.229
6.6 ± 0.239
1.258 ± 0.11
2.831 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here