Pediococcus stilesii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Pediococcus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1784 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2KZG1|A0A0R2KZG1_9LACO Capsular polysaccharide biosynthesis protein CpsC OS=Pediococcus stilesii OX=331679 GN=IV81_GL001013 PE=4 SV=1
MM1 pKa = 7.83LSEE4 pKa = 4.09TQAAADD10 pKa = 3.85VDD12 pKa = 4.28WNDD15 pKa = 3.25LVANGVQAALIRR27 pKa = 11.84LSHH30 pKa = 6.68GVTQDD35 pKa = 3.31LAAAAHH41 pKa = 6.43IANAKK46 pKa = 9.58KK47 pKa = 10.57VDD49 pKa = 3.69VHH51 pKa = 4.6VHH53 pKa = 6.57GYY55 pKa = 9.89HH56 pKa = 6.66EE57 pKa = 4.48YY58 pKa = 10.8EE59 pKa = 4.41GVDD62 pKa = 3.57DD63 pKa = 4.99EE64 pKa = 6.42VPFSLNNGVEE74 pKa = 4.06LGLAQGAYY82 pKa = 8.39MFLVGAPIDD91 pKa = 3.84AALGFANNWLSAGWKK106 pKa = 9.73IGTSDD111 pKa = 3.63VQDD114 pKa = 5.64DD115 pKa = 4.93YY116 pKa = 11.63YY117 pKa = 11.59QWITGADD124 pKa = 3.71EE125 pKa = 4.23PSVYY129 pKa = 10.71DD130 pKa = 3.8LWQFDD135 pKa = 3.67DD136 pKa = 3.52THH138 pKa = 8.61AVDD141 pKa = 5.42SSGQLLLDD149 pKa = 5.23PIDD152 pKa = 4.59PNPPIDD158 pKa = 3.96STTPTAPKK166 pKa = 9.43AGAYY170 pKa = 9.16VGYY173 pKa = 10.96GNDD176 pKa = 3.38TSGMLGGTSIGYY188 pKa = 7.94STDD191 pKa = 3.47GINFYY196 pKa = 11.32AVITPFGIIFRR207 pKa = 11.84DD208 pKa = 3.45GDD210 pKa = 3.79VEE212 pKa = 4.47RR213 pKa = 11.84MSNLLINKK221 pKa = 9.36LKK223 pKa = 10.5LQSPNGTVFNLAVSDD238 pKa = 4.42DD239 pKa = 4.09GVLSAVKK246 pKa = 10.29EE247 pKa = 4.18GDD249 pKa = 3.29GGG251 pKa = 3.75

Molecular weight: 26.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2L659|A0A0R2L659_9LACO HD domain-containing protein OS=Pediococcus stilesii OX=331679 GN=FEZ51_09540 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.69KK9 pKa = 7.77RR10 pKa = 11.84HH11 pKa = 4.99RR12 pKa = 11.84QRR14 pKa = 11.84VHH16 pKa = 6.07GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1784

0

1784

533784

44

1435

299.2

33.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.117 ± 0.064

0.364 ± 0.014

5.93 ± 0.054

6.119 ± 0.066

4.468 ± 0.047

6.763 ± 0.05

1.864 ± 0.024

8.005 ± 0.057

6.951 ± 0.06

9.476 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.028

5.295 ± 0.039

3.346 ± 0.029

4.174 ± 0.038

4.022 ± 0.043

6.26 ± 0.059

5.648 ± 0.038

7.095 ± 0.046

0.95 ± 0.02

3.367 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski