Sunn hemp leaf distortion virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9V4F3|B9V4F3_9GEMI Transcriptional activator protein OS=Sunn hemp leaf distortion virus OX=579113 GN=AC2 PE=3 SV=1
MM1 pKa = 7.69GFTEE5 pKa = 4.75CDD7 pKa = 3.06LRR9 pKa = 11.84HH10 pKa = 6.11PRR12 pKa = 11.84RR13 pKa = 11.84PTLLRR18 pKa = 11.84YY19 pKa = 9.29QSKK22 pKa = 10.67CSTGYY27 pKa = 9.42PRR29 pKa = 11.84RR30 pKa = 11.84GPDD33 pKa = 2.74VDD35 pKa = 4.57VFDD38 pKa = 4.84SLVDD42 pKa = 3.03IHH44 pKa = 8.34ISYY47 pKa = 10.31HH48 pKa = 6.31SPVHH52 pKa = 6.17DD53 pKa = 3.82HH54 pKa = 6.64GFTHH58 pKa = 7.6RR59 pKa = 11.84GTHH62 pKa = 5.81HH63 pKa = 6.84CSSSRR68 pKa = 11.84DD69 pKa = 3.01WRR71 pKa = 11.84VYY73 pKa = 10.83LGDD76 pKa = 3.69SKK78 pKa = 11.76SPLFQDD84 pKa = 3.24TRR86 pKa = 11.84APEE89 pKa = 4.13PSISHH94 pKa = 4.62QPRR97 pKa = 11.84YY98 pKa = 10.07KK99 pKa = 10.51NRR101 pKa = 11.84PNTVQSQPKK110 pKa = 9.36EE111 pKa = 4.2STGDD115 pKa = 3.48SQLFPILPNLDD126 pKa = 3.12VFTASDD132 pKa = 3.17WSFLLGLKK140 pKa = 10.1DD141 pKa = 3.38

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9V4F3|B9V4F3_9GEMI Transcriptional activator protein OS=Sunn hemp leaf distortion virus OX=579113 GN=AC2 PE=3 SV=1
MM1 pKa = 8.27DD2 pKa = 4.36SRR4 pKa = 11.84TGEE7 pKa = 4.83LITAAQAEE15 pKa = 4.36IGVFIWEE22 pKa = 4.36IQNPLYY28 pKa = 10.62FKK30 pKa = 10.53ILEE33 pKa = 4.31HH34 pKa = 5.88QNRR37 pKa = 11.84PFLINQDD44 pKa = 3.13TRR46 pKa = 11.84TVRR49 pKa = 11.84IQFNHH54 pKa = 6.13NLRR57 pKa = 11.84KK58 pKa = 9.81ALGIHH63 pKa = 5.83NCFLFYY69 pKa = 10.37RR70 pKa = 11.84IWMSSQPQTGRR81 pKa = 11.84FFWVLKK87 pKa = 9.86TEE89 pKa = 4.11VFKK92 pKa = 11.52YY93 pKa = 10.59LDD95 pKa = 3.42NLGIISINNVNRR107 pKa = 11.84AVDD110 pKa = 3.6HH111 pKa = 5.74VLWNVLQHH119 pKa = 6.28IVYY122 pKa = 10.03VDD124 pKa = 3.05QSYY127 pKa = 11.29SITFNIYY134 pKa = 9.93

Molecular weight: 15.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

275

134

141

137.5

16.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.545 ± 0.756

1.455 ± 0.451

6.545 ± 1.794

2.909 ± 0.524

6.182 ± 0.817

4.727 ± 0.635

5.091 ± 0.867

6.909 ± 2.731

3.273 ± 0.183

8.727 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.091 ± 0.256

5.455 ± 2.232

5.818 ± 2.282

5.455 ± 0.804

7.273 ± 0.83

8.364 ± 2.477

5.818 ± 0.379

6.182 ± 0.817

2.182 ± 0.512

4.0 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski