Capybara microvirus Cap1_SP_99
Average proteome isoelectric point is 5.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7P6|A0A4P8W7P6_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_99 OX=2584802 PE=4 SV=1
MM1 pKa = 7.79 ILRR4 pKa = 11.84 KK5 pKa = 9.69 GVNDD9 pKa = 3.61 TQTRR13 pKa = 11.84 PIGTTVYY20 pKa = 10.06 PPTLIVRR27 pKa = 11.84 TYY29 pKa = 9.83 VNEE32 pKa = 3.86 EE33 pKa = 4.15 GEE35 pKa = 4.3 EE36 pKa = 4.36 VSCVEE41 pKa = 4.55 SLDD44 pKa = 5.1 DD45 pKa = 5.86 IPFEE49 pKa = 4.35 DD50 pKa = 5.06 ANVGDD55 pKa = 4.16 YY56 pKa = 10.71 TLDD59 pKa = 3.53 ALFKK63 pKa = 10.97 AGINPRR69 pKa = 11.84 SVNFNSTVSNVDD81 pKa = 2.93 LHH83 pKa = 7.28 DD84 pKa = 4.62 ALNEE88 pKa = 3.89 INNLKK93 pKa = 10.35 VEE95 pKa = 4.2
Molecular weight: 10.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.344
IPC2_protein 4.253
IPC_protein 4.139
Toseland 3.961
ProMoST 4.228
Dawson 4.113
Bjellqvist 4.329
Wikipedia 4.012
Rodwell 3.973
Grimsley 3.872
Solomon 4.101
Lehninger 4.05
Nozaki 4.228
DTASelect 4.406
Thurlkill 3.999
EMBOSS 4.024
Sillero 4.253
Patrickios 3.694
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.181
Protein with the highest isoelectric point:
>tr|A0A4P8W4D4|A0A4P8W4D4_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_99 OX=2584802 PE=3 SV=1
MM1 pKa = 7.71 ISDD4 pKa = 4.66 SYY6 pKa = 10.71 ICSSIPLRR14 pKa = 11.84 SYY16 pKa = 11.35 YY17 pKa = 10.38 YY18 pKa = 10.67 DD19 pKa = 3.49 CFRR22 pKa = 11.84 DD23 pKa = 3.56 KK24 pKa = 11.18 SIIRR28 pKa = 11.84 VARR31 pKa = 11.84 RR32 pKa = 11.84 EE33 pKa = 3.97 NFGTSQLAQKK43 pKa = 10.3 EE44 pKa = 4.37 YY45 pKa = 10.01 TLRR48 pKa = 11.84 LYY50 pKa = 11.04 SEE52 pKa = 4.95 LKK54 pKa = 8.98 RR55 pKa = 11.84 TYY57 pKa = 9.76 ALHH60 pKa = 5.53 GRR62 pKa = 11.84 VYY64 pKa = 10.43 FYY66 pKa = 10.62 TLTYY70 pKa = 10.94 SDD72 pKa = 4.09 VNLPKK77 pKa = 10.68 YY78 pKa = 10.42 KK79 pKa = 10.48 GVSCVDD85 pKa = 3.55 YY86 pKa = 10.71 NHH88 pKa = 6.42 IRR90 pKa = 11.84 AMFHH94 pKa = 6.87 DD95 pKa = 5.29 FLYY98 pKa = 9.39 EE99 pKa = 3.74 QCEE102 pKa = 3.97 RR103 pKa = 11.84 AHH105 pKa = 6.2 CAKK108 pKa = 10.07 IKK110 pKa = 10.02 YY111 pKa = 9.52 FVVTEE116 pKa = 3.98 YY117 pKa = 11.6 GDD119 pKa = 3.84 GKK121 pKa = 10.68 GSRR124 pKa = 11.84 GKK126 pKa = 10.72 GNNPHH131 pKa = 5.87 FHH133 pKa = 6.73 VIFMFQPHH141 pKa = 6.57 ANVGKK146 pKa = 9.82 HH147 pKa = 3.7 LTGRR151 pKa = 11.84 KK152 pKa = 8.77 KK153 pKa = 10.62 SYY155 pKa = 10.27 MNIFFPPTPHH165 pKa = 7.1 QMLQYY170 pKa = 9.7 MRR172 pKa = 11.84 YY173 pKa = 7.91 YY174 pKa = 8.84 WQGNKK179 pKa = 7.5 ITNVYY184 pKa = 10.08 HH185 pKa = 7.4 DD186 pKa = 3.95 GSFDD190 pKa = 3.53 VEE192 pKa = 4.96 SYY194 pKa = 11.0 KK195 pKa = 10.41 RR196 pKa = 11.84 WQDD199 pKa = 3.19 LKK201 pKa = 11.32 YY202 pKa = 10.78 GFVKK206 pKa = 10.46 PGCLKK211 pKa = 10.36 GARR214 pKa = 11.84 NGAACGEE221 pKa = 4.15 VLPDD225 pKa = 4.27 NIDD228 pKa = 3.28 ALGYY232 pKa = 6.09 VTKK235 pKa = 10.88 YY236 pKa = 6.8 VTKK239 pKa = 8.83 DD240 pKa = 2.86 THH242 pKa = 5.36 KK243 pKa = 10.63 RR244 pKa = 11.84 RR245 pKa = 11.84 VEE247 pKa = 3.75 TEE249 pKa = 3.55 IYY251 pKa = 9.59 RR252 pKa = 11.84 RR253 pKa = 11.84 IYY255 pKa = 9.63 GEE257 pKa = 4.65 SYY259 pKa = 10.58 FDD261 pKa = 3.94 CANNLDD267 pKa = 3.75 LVYY270 pKa = 10.44 RR271 pKa = 11.84 CLRR274 pKa = 11.84 FSVEE278 pKa = 4.07 EE279 pKa = 4.01 KK280 pKa = 10.44 NKK282 pKa = 10.05 EE283 pKa = 3.84 FASEE287 pKa = 4.27 YY288 pKa = 10.58 EE289 pKa = 4.33 DD290 pKa = 3.38 IKK292 pKa = 11.57 GDD294 pKa = 3.58 VNYY297 pKa = 10.77 DD298 pKa = 3.78 GLSSIYY304 pKa = 10.81 DD305 pKa = 3.52 NLADD309 pKa = 3.65 VGKK312 pKa = 10.95 LEE314 pKa = 4.43 FSDD317 pKa = 4.93 SITEE321 pKa = 4.28 KK322 pKa = 10.88 LCSAYY327 pKa = 10.59 DD328 pKa = 3.79 NYY330 pKa = 11.35 LHH332 pKa = 6.59 SCSSRR337 pKa = 11.84 NVSPYY342 pKa = 10.34 FFTYY346 pKa = 10.56 CMNKK350 pKa = 9.46 GVRR353 pKa = 11.84 LLFPSLRR360 pKa = 11.84 DD361 pKa = 3.5 VQLNRR366 pKa = 11.84 IKK368 pKa = 10.91 HH369 pKa = 5.61 LFNKK373 pKa = 8.96 VFHH376 pKa = 6.9 SMLEE380 pKa = 4.2 EE381 pKa = 4.01 YY382 pKa = 10.71 QNEE385 pKa = 4.23 CVKK388 pKa = 10.75 YY389 pKa = 9.21 YY390 pKa = 10.61 RR391 pKa = 11.84 NRR393 pKa = 11.84 YY394 pKa = 5.1 STKK397 pKa = 9.91 VRR399 pKa = 11.84 ISQHH403 pKa = 5.73 FGDD406 pKa = 5.21 SIVEE410 pKa = 4.1 DD411 pKa = 4.36 FRR413 pKa = 11.84 EE414 pKa = 3.82 KK415 pKa = 10.81 ALQNRR420 pKa = 11.84 EE421 pKa = 3.97 LVYY424 pKa = 11.02 LLNINGKK431 pKa = 8.95 VNQKK435 pKa = 9.48 YY436 pKa = 8.83 YY437 pKa = 10.5 CGSYY441 pKa = 10.05 YY442 pKa = 10.11 YY443 pKa = 10.43 RR444 pKa = 11.84 KK445 pKa = 10.12 LFYY448 pKa = 10.61 KK449 pKa = 9.88 VVKK452 pKa = 10.47 GHH454 pKa = 7.06 DD455 pKa = 3.56 GSNKK459 pKa = 9.7 YY460 pKa = 9.86 VLNDD464 pKa = 3.44 FGIEE468 pKa = 4.01 YY469 pKa = 10.74 KK470 pKa = 10.25 MKK472 pKa = 10.0 TLDD475 pKa = 4.61 DD476 pKa = 4.52 NIEE479 pKa = 4.03 RR480 pKa = 11.84 QFAKK484 pKa = 10.05 YY485 pKa = 10.48 KK486 pKa = 10.26 RR487 pKa = 11.84 MLVSVYY493 pKa = 10.54 NDD495 pKa = 3.22 CNLYY499 pKa = 9.28 STFNDD504 pKa = 2.71 WRR506 pKa = 11.84 KK507 pKa = 10.52 KK508 pKa = 9.69 YY509 pKa = 10.47 GYY511 pKa = 10.32 DD512 pKa = 3.39 YY513 pKa = 10.82 GISFAGSFFSTLSSIGFSEE532 pKa = 3.88 ITEE535 pKa = 4.45 DD536 pKa = 4.81 DD537 pKa = 4.12 LLKK540 pKa = 10.81 AAIFKK545 pKa = 10.07 IVYY548 pKa = 8.75 RR549 pKa = 11.84 DD550 pKa = 3.48 RR551 pKa = 11.84 YY552 pKa = 7.43 TDD554 pKa = 3.42 CVGINHH560 pKa = 6.01 VDD562 pKa = 3.13 YY563 pKa = 8.24 FTRR566 pKa = 11.84 YY567 pKa = 7.76 RR568 pKa = 11.84 DD569 pKa = 3.62 YY570 pKa = 10.65 YY571 pKa = 8.6 EE572 pKa = 5.3 SYY574 pKa = 10.37 IIPHH578 pKa = 6.04 LVEE581 pKa = 4.85 NYY583 pKa = 10.58 SSLTSLYY590 pKa = 9.85 DD591 pKa = 3.3 INMKK595 pKa = 7.73 TVCAYY600 pKa = 9.19 PLSDD604 pKa = 3.5 NVVLSPYY611 pKa = 10.95 SKK613 pKa = 10.99 LCDD616 pKa = 3.52 LFEE619 pKa = 4.83 ALNDD623 pKa = 3.8 YY624 pKa = 10.33 IVYY627 pKa = 10.34 LRR629 pKa = 11.84 DD630 pKa = 3.62 IQWSNDD636 pKa = 3.09 YY637 pKa = 10.57 EE638 pKa = 4.23 EE639 pKa = 4.86 RR640 pKa = 11.84 SRR642 pKa = 11.84 LRR644 pKa = 11.84 KK645 pKa = 8.48 LHH647 pKa = 6.09 KK648 pKa = 9.66 RR649 pKa = 11.84 SEE651 pKa = 4.13 VEE653 pKa = 3.92 EE654 pKa = 3.96 YY655 pKa = 10.9 LVANRR660 pKa = 11.84 YY661 pKa = 7.76 LL662 pKa = 3.59
Molecular weight: 78.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.924
IPC2_protein 7.936
IPC_protein 7.732
Toseland 7.41
ProMoST 8.273
Dawson 8.448
Bjellqvist 8.712
Wikipedia 8.419
Rodwell 8.478
Grimsley 7.468
Solomon 8.478
Lehninger 8.478
Nozaki 8.741
DTASelect 8.492
Thurlkill 8.521
EMBOSS 8.609
Sillero 8.799
Patrickios 3.109
IPC_peptide 8.478
IPC2_peptide 7.644
IPC2.peptide.svr19 7.968
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1703
95
662
425.8
48.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.166 ± 1.419
1.644 ± 0.531
6.166 ± 0.322
4.815 ± 0.607
4.521 ± 0.448
6.459 ± 0.746
1.82 ± 0.516
5.578 ± 0.324
5.872 ± 0.97
7.575 ± 0.345
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.642 ± 0.487
6.753 ± 0.562
3.23 ± 0.627
3.699 ± 0.869
4.756 ± 0.886
8.28 ± 0.711
5.52 ± 0.929
6.577 ± 0.918
0.998 ± 0.203
6.929 ± 1.744
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here