Escherichia phage TL-2011b

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Uetakevirus; unclassified Uetakevirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9L6B6|G9L6B6_9CAUD Uncharacterized protein OS=Escherichia phage TL-2011b OX=1124654 PE=4 SV=1
MM1 pKa = 8.29DD2 pKa = 4.56YY3 pKa = 11.03SQLSDD8 pKa = 3.57FEE10 pKa = 4.99INRR13 pKa = 11.84MVGDD17 pKa = 4.66IIFKK21 pKa = 10.13GLWACKK27 pKa = 10.03PEE29 pKa = 4.15TSGNNTNKK37 pKa = 9.6WYY39 pKa = 10.71YY40 pKa = 11.04GNADD44 pKa = 3.57TTFEE48 pKa = 4.71PLTPLPDD55 pKa = 3.57YY56 pKa = 11.11CNDD59 pKa = 5.33PIASWPIIEE68 pKa = 5.04KK69 pKa = 10.44YY70 pKa = 10.24RR71 pKa = 11.84ISILDD76 pKa = 4.19QLTEE80 pKa = 3.86WCVDD84 pKa = 3.41AKK86 pKa = 10.34DD87 pKa = 3.55VRR89 pKa = 11.84PIFDD93 pKa = 3.66TRR95 pKa = 11.84PLRR98 pKa = 11.84AAMIVFLMMQEE109 pKa = 4.11NQNGG113 pKa = 3.31

Molecular weight: 13.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9L6E8|G9L6E8_9CAUD Putative endolysin OS=Escherichia phage TL-2011b OX=1124654 PE=4 SV=1
MM1 pKa = 6.45VTHH4 pKa = 7.58DD5 pKa = 5.2LFLLITNAVICTGIAIRR22 pKa = 11.84VVTFQRR28 pKa = 11.84NGSQHH33 pKa = 6.13RR34 pKa = 11.84RR35 pKa = 11.84WGGWIAYY42 pKa = 7.63FLIVAAASIPVRR54 pKa = 11.84VVYY57 pKa = 9.65AIWLRR62 pKa = 11.84TPMAVDD68 pKa = 3.96LSEE71 pKa = 4.6VIINAVMLAAVIKK84 pKa = 9.58TRR86 pKa = 11.84GNVVQIFKK94 pKa = 9.02ITRR97 pKa = 11.84SKK99 pKa = 10.99HH100 pKa = 4.37GDD102 pKa = 2.95

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12409

36

1004

217.7

24.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.437 ± 0.406

1.064 ± 0.156

5.859 ± 0.24

6.366 ± 0.334

3.594 ± 0.284

7.14 ± 0.346

1.507 ± 0.172

5.778 ± 0.367

5.552 ± 0.28

7.591 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.046 ± 0.239

4.827 ± 0.245

4.07 ± 0.232

4.642 ± 0.406

5.786 ± 0.288

6.632 ± 0.312

5.681 ± 0.308

6.584 ± 0.333

1.668 ± 0.154

3.175 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski