Rhodovulum sp. 12E13

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum; unclassified Rhodovulum

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369QZL9|A0A369QZL9_9RHOB Uncharacterized protein OS=Rhodovulum sp. 12E13 OX=2203891 GN=DLJ49_08750 PE=4 SV=1
MM1 pKa = 7.8LFRR4 pKa = 11.84IAKK7 pKa = 9.25AATSAVALIAAVPASAQDD25 pKa = 3.5QEE27 pKa = 4.3QDD29 pKa = 3.64GVEE32 pKa = 4.25LLSNWSYY39 pKa = 11.89DD40 pKa = 3.49PLYY43 pKa = 11.15AEE45 pKa = 4.81GWSVEE50 pKa = 3.8NMFDD54 pKa = 3.02MTEE57 pKa = 3.9IVDD60 pKa = 3.69AAGEE64 pKa = 4.15EE65 pKa = 4.15IGDD68 pKa = 3.74VEE70 pKa = 4.69NVLFANDD77 pKa = 4.22GEE79 pKa = 4.52VLGIIAQVGGFWDD92 pKa = 3.23ILDD95 pKa = 3.63THH97 pKa = 6.86VYY99 pKa = 9.79VPWDD103 pKa = 4.02EE104 pKa = 4.23VTMEE108 pKa = 4.49AGIQRR113 pKa = 11.84MGIPVTEE120 pKa = 4.25EE121 pKa = 3.7TVDD124 pKa = 3.81DD125 pKa = 4.21YY126 pKa = 11.94DD127 pKa = 4.11VFGDD131 pKa = 4.02FDD133 pKa = 4.72FDD135 pKa = 3.62EE136 pKa = 4.3QVIDD140 pKa = 4.21EE141 pKa = 4.68SDD143 pKa = 3.23TGEE146 pKa = 4.19TQPVDD151 pKa = 3.66DD152 pKa = 5.54DD153 pKa = 4.16LAAGAGVFKK162 pKa = 10.58ATDD165 pKa = 3.52LVGDD169 pKa = 3.71YY170 pKa = 10.91AYY172 pKa = 10.93LSDD175 pKa = 3.72GARR178 pKa = 11.84YY179 pKa = 9.75GYY181 pKa = 10.48VADD184 pKa = 6.49LIVQDD189 pKa = 4.14GAISAVVTDD198 pKa = 4.2AAAYY202 pKa = 7.96GRR204 pKa = 11.84PGYY207 pKa = 9.6YY208 pKa = 10.06AYY210 pKa = 9.72PYY212 pKa = 9.63SYY214 pKa = 10.0RR215 pKa = 11.84GAMSPRR221 pKa = 11.84YY222 pKa = 8.35EE223 pKa = 3.93IPYY226 pKa = 10.48DD227 pKa = 3.49PVEE230 pKa = 4.94IDD232 pKa = 3.07TLEE235 pKa = 4.27NFDD238 pKa = 4.23YY239 pKa = 11.15EE240 pKa = 4.38QLQSRR245 pKa = 11.84AMPWRR250 pKa = 11.84SATSPGRR257 pKa = 11.84RR258 pKa = 11.84PSSCAHH264 pKa = 6.13GPAVGLLSGTKK275 pKa = 6.76TTSGRR280 pKa = 11.84RR281 pKa = 11.84LAGQGGCPAPYY292 pKa = 10.0NPCEE296 pKa = 4.64DD297 pKa = 3.7GGAHH301 pKa = 6.58HH302 pKa = 6.89EE303 pKa = 4.2WAVLLGEE310 pKa = 4.25RR311 pKa = 11.84TSPRR315 pKa = 11.84RR316 pKa = 11.84VRR318 pKa = 3.66

Molecular weight: 34.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369QR66|A0A369QR66_9RHOB Uncharacterized protein OS=Rhodovulum sp. 12E13 OX=2203891 GN=DLJ49_20180 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4102

0

4102

1297611

31

2794

316.3

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.702 ± 0.07

0.829 ± 0.012

5.841 ± 0.029

6.286 ± 0.034

3.373 ± 0.025

9.39 ± 0.037

2.031 ± 0.02

4.206 ± 0.03

1.966 ± 0.029

10.157 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.019

1.835 ± 0.018

5.798 ± 0.032

2.626 ± 0.019

8.082 ± 0.046

4.374 ± 0.024

5.195 ± 0.026

7.503 ± 0.035

1.448 ± 0.017

1.93 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski