Lynx canadensis faeces associated genomovirus CL1 148
Average proteome isoelectric point is 7.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJ94|A0A2Z5CJ94_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL1 148 OX=2219123 PE=3 SV=1
MM1 pKa = 7.56 SFHH4 pKa = 7.26 CNAQYY9 pKa = 11.23 FLVTYY14 pKa = 7.6 AQCGDD19 pKa = 4.0 LDD21 pKa = 3.71 PWSVMEE27 pKa = 4.75 RR28 pKa = 11.84 FSTLGAEE35 pKa = 4.5 CIIARR40 pKa = 11.84 EE41 pKa = 3.96 LHH43 pKa = 6.59 ADD45 pKa = 3.73 LGLHH49 pKa = 5.87 LHH51 pKa = 6.5 CFVDD55 pKa = 4.63 FGRR58 pKa = 11.84 KK59 pKa = 8.02 FRR61 pKa = 11.84 SRR63 pKa = 11.84 KK64 pKa = 7.68 TDD66 pKa = 2.79 IFDD69 pKa = 3.68 VGSSHH74 pKa = 7.51 PNIEE78 pKa = 4.23 PSRR81 pKa = 11.84 GTPWAGYY88 pKa = 10.35 DD89 pKa = 3.62 YY90 pKa = 10.72 AIKK93 pKa = 10.89 DD94 pKa = 3.47 GDD96 pKa = 4.3 VVCGGLARR104 pKa = 11.84 PRR106 pKa = 11.84 QPRR109 pKa = 11.84 SKK111 pKa = 9.31 STRR114 pKa = 11.84 NDD116 pKa = 2.56 VHH118 pKa = 7.69 QYY120 pKa = 9.72 TEE122 pKa = 3.84 ITNADD127 pKa = 4.18 DD128 pKa = 5.43 IEE130 pKa = 4.78 HH131 pKa = 6.04 FWEE134 pKa = 4.74 LLHH137 pKa = 7.77 HH138 pKa = 7.26 LDD140 pKa = 3.99 PKK142 pKa = 8.83 TAVVNFVSATKK153 pKa = 9.94 YY154 pKa = 10.69 AEE156 pKa = 3.91 ARR158 pKa = 11.84 FADD161 pKa = 4.05 VEE163 pKa = 4.22 PGYY166 pKa = 10.37 EE167 pKa = 3.7 PRR169 pKa = 11.84 RR170 pKa = 11.84 RR171 pKa = 11.84 QDD173 pKa = 3.51 FVAGDD178 pKa = 3.71 DD179 pKa = 4.01 DD180 pKa = 4.95 GRR182 pKa = 11.84 DD183 pKa = 3.48 AVKK186 pKa = 10.75 SLVLFGEE193 pKa = 4.38 PLTGKK198 pKa = 8.47 TDD200 pKa = 3.28 WARR203 pKa = 11.84 CLGNHH208 pKa = 6.21 VYY210 pKa = 10.71 TMGIVSGDD218 pKa = 3.57 QMLKK222 pKa = 9.94 IANPDD227 pKa = 2.76 VKK229 pKa = 10.96 YY230 pKa = 10.96 AVFDD234 pKa = 4.95 DD235 pKa = 3.28 IRR237 pKa = 11.84 GGIKK241 pKa = 9.99 FFPSFKK247 pKa = 10.33 EE248 pKa = 3.72 WLGCQAYY255 pKa = 7.88 VTVTKK260 pKa = 10.25 KK261 pKa = 10.8 YY262 pKa = 10.52 KK263 pKa = 9.98 EE264 pKa = 4.1 PKK266 pKa = 8.34 LMKK269 pKa = 9.51 WGRR272 pKa = 11.84 PSIWLSNKK280 pKa = 9.96 DD281 pKa = 3.78 PRR283 pKa = 11.84 TEE285 pKa = 4.05 MDD287 pKa = 3.7 PSDD290 pKa = 4.75 ANWLEE295 pKa = 4.1 ANCTFIEE302 pKa = 4.15 INTPIFHH309 pKa = 7.44 ANTT312 pKa = 3.57
Molecular weight: 35.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.719
IPC2_protein 5.664
IPC_protein 5.715
Toseland 6.109
ProMoST 6.071
Dawson 5.995
Bjellqvist 6.008
Wikipedia 6.008
Rodwell 5.982
Grimsley 6.249
Solomon 5.995
Lehninger 5.995
Nozaki 6.262
DTASelect 6.465
Thurlkill 6.465
EMBOSS 6.427
Sillero 6.364
Patrickios 4.075
IPC_peptide 6.008
IPC2_peptide 6.364
IPC2.peptide.svr19 6.311
Protein with the highest isoelectric point:
>tr|A0A2Z5CJ94|A0A2Z5CJ94_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL1 148 OX=2219123 PE=3 SV=1
MM1 pKa = 7.86 PYY3 pKa = 8.52 STKK6 pKa = 10.18 RR7 pKa = 11.84 GSRR10 pKa = 11.84 RR11 pKa = 11.84 SYY13 pKa = 8.95 TKK15 pKa = 9.97 RR16 pKa = 11.84 GGFKK20 pKa = 10.39 RR21 pKa = 11.84 RR22 pKa = 11.84 TAPKK26 pKa = 9.54 RR27 pKa = 11.84 RR28 pKa = 11.84 TSYY31 pKa = 10.1 RR32 pKa = 11.84 RR33 pKa = 11.84 SRR35 pKa = 11.84 YY36 pKa = 9.27 SRR38 pKa = 11.84 PKK40 pKa = 8.31 RR41 pKa = 11.84 TVMPKK46 pKa = 10.25 KK47 pKa = 10.42 RR48 pKa = 11.84 ILNLTSTKK56 pKa = 10.2 KK57 pKa = 10.43 RR58 pKa = 11.84 NTMLQYY64 pKa = 11.51 ANTSTSGGGSVTIGPGPYY82 pKa = 10.0 LVTGALGGFSIFCPTAMDD100 pKa = 3.69 LTEE103 pKa = 5.03 KK104 pKa = 10.62 SGANNSVVNVAEE116 pKa = 4.51 RR117 pKa = 11.84 NSTTCFMRR125 pKa = 11.84 GFSEE129 pKa = 4.03 NLRR132 pKa = 11.84 IQTSTGIPWFWRR144 pKa = 11.84 RR145 pKa = 11.84 ITFCSKK151 pKa = 10.42 SGVFGVFDD159 pKa = 4.21 SGDD162 pKa = 3.5 TPTQTNAGEE171 pKa = 4.1 TSYY174 pKa = 11.88 VDD176 pKa = 3.32 TTNGMEE182 pKa = 4.48 RR183 pKa = 11.84 LWFNQNVNSAPATVTSIQAIMFKK206 pKa = 10.66 GQYY209 pKa = 9.22 NKK211 pKa = 10.95 DD212 pKa = 3.03 WVDD215 pKa = 3.5 PLTAPLDD222 pKa = 3.65 TARR225 pKa = 11.84 IDD227 pKa = 3.69 VKK229 pKa = 11.25 SDD231 pKa = 2.94 TTKK234 pKa = 10.86 CIRR237 pKa = 11.84 SGNASGTVFEE247 pKa = 4.74 KK248 pKa = 10.73 KK249 pKa = 9.21 CWYY252 pKa = 9.53 PMNKK256 pKa = 9.47 NLVYY260 pKa = 10.83 DD261 pKa = 4.46 DD262 pKa = 5.1 DD263 pKa = 4.63 EE264 pKa = 6.36 SGEE267 pKa = 4.47 GEE269 pKa = 4.17 VARR272 pKa = 11.84 YY273 pKa = 7.82 TSVVDD278 pKa = 3.53 KK279 pKa = 11.06 KK280 pKa = 11.64 GMGDD284 pKa = 3.57 YY285 pKa = 10.55 FIVDD289 pKa = 3.52 IFVVGTGGATADD301 pKa = 3.56 QLQVTGTASMYY312 pKa = 8.7 WHH314 pKa = 7.06 EE315 pKa = 4.33 KK316 pKa = 9.44
Molecular weight: 35.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.193
IPC2_protein 9.268
IPC_protein 9.268
Toseland 9.882
ProMoST 9.648
Dawson 10.116
Bjellqvist 9.823
Wikipedia 10.292
Rodwell 10.467
Grimsley 10.189
Solomon 10.145
Lehninger 10.101
Nozaki 9.926
DTASelect 9.794
Thurlkill 9.955
EMBOSS 10.292
Sillero 10.043
Patrickios 9.545
IPC_peptide 10.131
IPC2_peptide 8.565
IPC2.peptide.svr19 8.18
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
628
312
316
314.0
35.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.369 ± 0.775
2.07 ± 0.382
6.847 ± 1.396
4.14 ± 0.764
5.096 ± 0.521
8.28 ± 0.95
1.911 ± 1.248
4.299 ± 0.393
6.21 ± 0.341
5.096 ± 1.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.548 ± 0.483
4.618 ± 0.596
4.777 ± 0.519
2.389 ± 0.112
6.847 ± 0.585
7.006 ± 1.451
8.758 ± 2.31
6.688 ± 0.215
2.229 ± 0.259
3.822 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here