Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3982 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A6L3C7|A6L3C7_BACV8 Uncharacterized protein OS=Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154) OX=435590 GN=BVU_2534 PE=4 SV=1
MM1 pKa = 7.28 EE2 pKa = 5.61 AVTLSEE8 pKa = 3.54 ARR10 pKa = 11.84 VYY12 pKa = 10.88 VGTYY16 pKa = 9.56 NKK18 pKa = 10.08 YY19 pKa = 10.6 NNGSLFGKK27 pKa = 9.26 WLDD30 pKa = 3.72 LSDD33 pKa = 5.06 YY34 pKa = 10.69 SDD36 pKa = 3.45 KK37 pKa = 11.77 DD38 pKa = 3.52 EE39 pKa = 4.48 FLEE42 pKa = 4.84 ACRR45 pKa = 11.84 EE46 pKa = 4.05 LHH48 pKa = 6.85 KK49 pKa = 11.19 DD50 pKa = 3.48 EE51 pKa = 5.31 EE52 pKa = 4.7 DD53 pKa = 3.94 PEE55 pKa = 5.94 LMFQDD60 pKa = 3.85 IEE62 pKa = 4.54 NIPEE66 pKa = 4.03 ALISEE71 pKa = 4.51 SWLSDD76 pKa = 2.93 KK77 pKa = 10.81 FFEE80 pKa = 5.53 LRR82 pKa = 11.84 DD83 pKa = 4.26 AIEE86 pKa = 4.48 KK87 pKa = 10.62 LSEE90 pKa = 4.15 TEE92 pKa = 3.79 QEE94 pKa = 5.56 AFFVWCDD101 pKa = 3.32 HH102 pKa = 6.55 HH103 pKa = 8.25 NSDD106 pKa = 5.34 ISEE109 pKa = 4.05 EE110 pKa = 4.1 DD111 pKa = 3.33 ADD113 pKa = 4.29 DD114 pKa = 4.21 LVSSFEE120 pKa = 4.54 DD121 pKa = 4.35 EE122 pKa = 4.35 YY123 pKa = 11.27 QGEE126 pKa = 4.44 YY127 pKa = 10.22 KK128 pKa = 10.73 DD129 pKa = 4.14 EE130 pKa = 3.79 EE131 pKa = 5.05 DD132 pKa = 3.37 YY133 pKa = 11.06 AYY135 pKa = 10.1 EE136 pKa = 4.09 IVEE139 pKa = 4.15 EE140 pKa = 5.11 CYY142 pKa = 10.75 DD143 pKa = 3.68 LPEE146 pKa = 4.02 FAKK149 pKa = 10.18 TYY151 pKa = 10.45 FDD153 pKa = 3.79 YY154 pKa = 11.39 SAFARR159 pKa = 11.84 DD160 pKa = 5.2 LFMTDD165 pKa = 2.67 YY166 pKa = 11.06 WMDD169 pKa = 3.08 NGFVFRR175 pKa = 11.84 CAA177 pKa = 3.58
Molecular weight: 21.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.63
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.088
Thurlkill 3.668
EMBOSS 3.706
Sillero 3.948
Patrickios 0.922
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|A6KYZ6|A6KYZ6_BACV8 Mobilisation protein C OS=Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154) OX=435590 GN=BVU_0965 PE=4 SV=1
MM1 pKa = 7.0 GQYY4 pKa = 9.82 HH5 pKa = 5.94 RR6 pKa = 11.84 QGEE9 pKa = 4.17 QGHH12 pKa = 5.75 AKK14 pKa = 10.13 GRR16 pKa = 11.84 IRR18 pKa = 11.84 RR19 pKa = 11.84 TGLVCRR25 pKa = 11.84 LCIAGRR31 pKa = 11.84 SRR33 pKa = 11.84 GVRR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.71 EE39 pKa = 3.49 CHH41 pKa = 6.16 FRR43 pKa = 11.84 YY44 pKa = 9.89 QPVDD48 pKa = 3.0 MRR50 pKa = 11.84 YY51 pKa = 9.97 RR52 pKa = 11.84 IRR54 pKa = 11.84 KK55 pKa = 8.67 GLEE58 pKa = 3.9 SPCMIRR64 pKa = 11.84 GLLSGDD70 pKa = 2.97 YY71 pKa = 9.65 WIFVGCCSAAFVLFFLGIRR90 pKa = 11.84 AGISAGNWNLPVLVLVISLPTLPLLLYY117 pKa = 9.93 KK118 pKa = 10.47 LKK120 pKa = 10.79 RR121 pKa = 11.84 KK122 pKa = 9.24 ARR124 pKa = 11.84 PQKK127 pKa = 10.32 FKK129 pKa = 10.52 EE130 pKa = 4.06 TGKK133 pKa = 10.56 EE134 pKa = 3.79 INISNLDD141 pKa = 3.4 INRR144 pKa = 11.84 VLTKK148 pKa = 10.21 KK149 pKa = 9.29 EE150 pKa = 3.75 HH151 pKa = 5.52 NEE153 pKa = 3.12 IRR155 pKa = 11.84 RR156 pKa = 11.84 GIQHH160 pKa = 6.08 TT161 pKa = 3.73
Molecular weight: 18.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.794
IPC_protein 10.672
Toseland 10.862
ProMoST 10.599
Dawson 10.935
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 11.14
Grimsley 10.979
Solomon 11.096
Lehninger 11.052
Nozaki 10.862
DTASelect 10.672
Thurlkill 10.847
EMBOSS 11.272
Sillero 10.877
Patrickios 10.862
IPC_peptide 11.096
IPC2_peptide 9.911
IPC2.peptide.svr19 8.484
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3982
0
3982
1490209
31
1892
374.2
42.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.864 ± 0.037
1.288 ± 0.015
5.472 ± 0.026
6.618 ± 0.035
4.568 ± 0.024
6.865 ± 0.036
1.971 ± 0.017
6.878 ± 0.033
6.789 ± 0.03
9.017 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.828 ± 0.018
5.232 ± 0.03
3.729 ± 0.018
3.422 ± 0.019
4.653 ± 0.027
5.995 ± 0.029
5.586 ± 0.028
6.328 ± 0.029
1.344 ± 0.015
4.554 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here