Amino acid dipepetide frequency for Bacillus phage PM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.966AlaAla: 5.966 ± 0.777
0.066AlaCys: 0.066 ± 0.061
3.803AlaAsp: 3.803 ± 0.545
6.098AlaGlu: 6.098 ± 0.588
3.213AlaPhe: 3.213 ± 0.407
4.327AlaGly: 4.327 ± 0.619
0.59AlaHis: 0.59 ± 0.208
4.131AlaIle: 4.131 ± 0.779
5.573AlaLys: 5.573 ± 0.657
5.835AlaLeu: 5.835 ± 0.565
1.967AlaMet: 1.967 ± 0.337
3.409AlaAsn: 3.409 ± 0.566
1.705AlaPro: 1.705 ± 0.333
2.229AlaGln: 2.229 ± 0.403
2.36AlaArg: 2.36 ± 0.363
3.475AlaSer: 3.475 ± 0.5
2.885AlaThr: 2.885 ± 0.441
5.573AlaVal: 5.573 ± 0.651
0.59AlaTrp: 0.59 ± 0.19
2.36AlaTyr: 2.36 ± 0.401
0.0AlaXaa: 0.0 ± 0.0
Cys
0.262CysAla: 0.262 ± 0.134
0.066CysCys: 0.066 ± 0.067
0.262CysAsp: 0.262 ± 0.152
0.525CysGlu: 0.525 ± 0.202
0.197CysPhe: 0.197 ± 0.1
0.393CysGly: 0.393 ± 0.159
0.131CysHis: 0.131 ± 0.094
0.459CysIle: 0.459 ± 0.198
0.328CysLys: 0.328 ± 0.149
0.328CysLeu: 0.328 ± 0.161
0.131CysMet: 0.131 ± 0.098
0.393CysAsn: 0.393 ± 0.158
0.197CysPro: 0.197 ± 0.109
0.328CysGln: 0.328 ± 0.143
0.525CysArg: 0.525 ± 0.233
0.525CysSer: 0.525 ± 0.181
0.328CysThr: 0.328 ± 0.141
0.328CysVal: 0.328 ± 0.136
0.131CysTrp: 0.131 ± 0.077
0.525CysTyr: 0.525 ± 0.21
0.0CysXaa: 0.0 ± 0.0
Asp
3.606AspAla: 3.606 ± 0.486
0.262AspCys: 0.262 ± 0.128
3.803AspAsp: 3.803 ± 0.489
5.507AspGlu: 5.507 ± 0.823
3.147AspPhe: 3.147 ± 0.428
5.442AspGly: 5.442 ± 0.542
0.721AspHis: 0.721 ± 0.296
3.868AspIle: 3.868 ± 0.538
4.393AspLys: 4.393 ± 0.524
4.327AspLeu: 4.327 ± 0.539
1.574AspMet: 1.574 ± 0.393
3.409AspAsn: 3.409 ± 0.498
2.229AspPro: 2.229 ± 0.429
1.705AspGln: 1.705 ± 0.327
2.754AspArg: 2.754 ± 0.427
2.95AspSer: 2.95 ± 0.338
4.458AspThr: 4.458 ± 0.44
3.672AspVal: 3.672 ± 0.491
0.983AspTrp: 0.983 ± 0.261
2.295AspTyr: 2.295 ± 0.392
0.0AspXaa: 0.0 ± 0.0
Glu
4.524GluAla: 4.524 ± 0.543
0.59GluCys: 0.59 ± 0.205
4.262GluAsp: 4.262 ± 0.576
8.065GluGlu: 8.065 ± 1.264
3.082GluPhe: 3.082 ± 0.416
4.393GluGly: 4.393 ± 0.684
1.049GluHis: 1.049 ± 0.284
6.753GluIle: 6.753 ± 0.7
7.212GluLys: 7.212 ± 0.81
6.36GluLeu: 6.36 ± 0.694
3.082GluMet: 3.082 ± 0.477
5.049GluAsn: 5.049 ± 0.536
2.819GluPro: 2.819 ± 0.628
3.147GluGln: 3.147 ± 0.473
3.672GluArg: 3.672 ± 0.651
3.344GluSer: 3.344 ± 0.392
4.131GluThr: 4.131 ± 0.495
4.917GluVal: 4.917 ± 0.556
0.852GluTrp: 0.852 ± 0.192
3.344GluTyr: 3.344 ± 0.55
0.0GluXaa: 0.0 ± 0.0
Phe
2.229PheAla: 2.229 ± 0.443
0.262PheCys: 0.262 ± 0.128
2.95PheAsp: 2.95 ± 0.412
3.213PheGlu: 3.213 ± 0.433
2.098PhePhe: 2.098 ± 0.366
3.278PheGly: 3.278 ± 0.461
0.328PheHis: 0.328 ± 0.139
2.623PheIle: 2.623 ± 0.342
4.458PheLys: 4.458 ± 0.51
2.885PheLeu: 2.885 ± 0.441
1.508PheMet: 1.508 ± 0.348
2.426PheAsn: 2.426 ± 0.374
1.115PhePro: 1.115 ± 0.33
0.852PheGln: 0.852 ± 0.252
1.246PheArg: 1.246 ± 0.238
2.754PheSer: 2.754 ± 0.553
3.016PheThr: 3.016 ± 0.531
2.819PheVal: 2.819 ± 0.419
0.393PheTrp: 0.393 ± 0.179
1.77PheTyr: 1.77 ± 0.329
0.0PheXaa: 0.0 ± 0.0
Gly
4.327GlyAla: 4.327 ± 0.711
0.59GlyCys: 0.59 ± 0.253
4.393GlyAsp: 4.393 ± 0.582
5.245GlyGlu: 5.245 ± 0.597
3.016GlyPhe: 3.016 ± 0.541
5.049GlyGly: 5.049 ± 0.827
1.18GlyHis: 1.18 ± 0.244
4.59GlyIle: 4.59 ± 0.661
5.835GlyLys: 5.835 ± 0.568
6.229GlyLeu: 6.229 ± 0.862
1.705GlyMet: 1.705 ± 0.349
3.147GlyAsn: 3.147 ± 0.45
1.967GlyPro: 1.967 ± 0.517
2.295GlyGln: 2.295 ± 0.436
2.885GlyArg: 2.885 ± 0.499
4.852GlySer: 4.852 ± 0.679
3.409GlyThr: 3.409 ± 0.537
4.524GlyVal: 4.524 ± 0.611
0.852GlyTrp: 0.852 ± 0.24
2.754GlyTyr: 2.754 ± 0.449
0.0GlyXaa: 0.0 ± 0.0
His
0.852HisAla: 0.852 ± 0.239
0.0HisCys: 0.0 ± 0.0
1.18HisAsp: 1.18 ± 0.245
1.049HisGlu: 1.049 ± 0.26
1.442HisPhe: 1.442 ± 0.341
1.246HisGly: 1.246 ± 0.251
0.197HisHis: 0.197 ± 0.112
1.18HisIle: 1.18 ± 0.311
0.983HisLys: 0.983 ± 0.265
1.442HisLeu: 1.442 ± 0.359
0.525HisMet: 0.525 ± 0.204
0.59HisAsn: 0.59 ± 0.188
1.049HisPro: 1.049 ± 0.238
0.393HisGln: 0.393 ± 0.187
0.59HisArg: 0.59 ± 0.192
0.721HisSer: 0.721 ± 0.211
0.787HisThr: 0.787 ± 0.26
0.721HisVal: 0.721 ± 0.187
0.262HisTrp: 0.262 ± 0.126
0.459HisTyr: 0.459 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
4.852IleAla: 4.852 ± 0.702
0.656IleCys: 0.656 ± 0.201
4.59IleAsp: 4.59 ± 0.668
5.114IleGlu: 5.114 ± 0.89
2.36IlePhe: 2.36 ± 0.346
4.786IleGly: 4.786 ± 0.592
1.442IleHis: 1.442 ± 0.275
3.868IleIle: 3.868 ± 0.445
6.884IleLys: 6.884 ± 0.577
4.327IleLeu: 4.327 ± 0.504
1.901IleMet: 1.901 ± 0.335
4.59IleAsn: 4.59 ± 0.532
2.491IlePro: 2.491 ± 0.41
2.557IleGln: 2.557 ± 0.328
3.082IleArg: 3.082 ± 0.366
4.131IleSer: 4.131 ± 0.511
4.721IleThr: 4.721 ± 0.547
3.606IleVal: 3.606 ± 0.497
0.59IleTrp: 0.59 ± 0.214
2.491IleTyr: 2.491 ± 0.414
0.0IleXaa: 0.0 ± 0.0
Lys
6.753LysAla: 6.753 ± 0.722
0.59LysCys: 0.59 ± 0.238
5.901LysAsp: 5.901 ± 0.637
8.065LysGlu: 8.065 ± 0.932
2.754LysPhe: 2.754 ± 0.583
5.639LysGly: 5.639 ± 0.631
1.246LysHis: 1.246 ± 0.302
6.753LysIle: 6.753 ± 0.767
10.097LysLys: 10.097 ± 1.134
4.852LysLeu: 4.852 ± 0.579
2.295LysMet: 2.295 ± 0.394
5.049LysAsn: 5.049 ± 0.701
2.754LysPro: 2.754 ± 0.463
3.475LysGln: 3.475 ± 0.474
4.852LysArg: 4.852 ± 0.63
5.376LysSer: 5.376 ± 0.624
4.786LysThr: 4.786 ± 0.51
4.721LysVal: 4.721 ± 0.577
1.049LysTrp: 1.049 ± 0.318
3.606LysTyr: 3.606 ± 0.723
0.0LysXaa: 0.0 ± 0.0
Leu
4.917LeuAla: 4.917 ± 0.681
0.197LeuCys: 0.197 ± 0.135
5.049LeuAsp: 5.049 ± 0.472
5.376LeuGlu: 5.376 ± 0.533
3.475LeuPhe: 3.475 ± 0.468
3.541LeuGly: 3.541 ± 0.544
1.049LeuHis: 1.049 ± 0.27
4.196LeuIle: 4.196 ± 0.507
6.425LeuLys: 6.425 ± 0.634
5.966LeuLeu: 5.966 ± 0.591
1.508LeuMet: 1.508 ± 0.299
4.262LeuAsn: 4.262 ± 0.54
2.754LeuPro: 2.754 ± 0.403
2.557LeuGln: 2.557 ± 0.42
2.623LeuArg: 2.623 ± 0.451
5.704LeuSer: 5.704 ± 0.561
4.524LeuThr: 4.524 ± 0.608
3.606LeuVal: 3.606 ± 0.472
0.918LeuTrp: 0.918 ± 0.226
3.016LeuTyr: 3.016 ± 0.486
0.0LeuXaa: 0.0 ± 0.0
Met
1.967MetAla: 1.967 ± 0.351
0.328MetCys: 0.328 ± 0.124
1.377MetAsp: 1.377 ± 0.324
1.508MetGlu: 1.508 ± 0.322
0.918MetPhe: 0.918 ± 0.241
1.639MetGly: 1.639 ± 0.321
0.328MetHis: 0.328 ± 0.155
2.623MetIle: 2.623 ± 0.44
3.737MetLys: 3.737 ± 0.574
1.508MetLeu: 1.508 ± 0.356
0.787MetMet: 0.787 ± 0.228
2.623MetAsn: 2.623 ± 0.374
1.311MetPro: 1.311 ± 0.248
0.918MetGln: 0.918 ± 0.284
1.442MetArg: 1.442 ± 0.295
1.705MetSer: 1.705 ± 0.32
2.098MetThr: 2.098 ± 0.298
1.574MetVal: 1.574 ± 0.238
0.262MetTrp: 0.262 ± 0.127
0.852MetTyr: 0.852 ± 0.212
0.0MetXaa: 0.0 ± 0.0
Asn
3.672AsnAla: 3.672 ± 0.625
0.525AsnCys: 0.525 ± 0.184
3.147AsnAsp: 3.147 ± 0.401
3.606AsnGlu: 3.606 ± 0.465
1.967AsnPhe: 1.967 ± 0.318
5.704AsnGly: 5.704 ± 0.572
1.115AsnHis: 1.115 ± 0.275
3.868AsnIle: 3.868 ± 0.587
4.917AsnLys: 4.917 ± 0.63
4.393AsnLeu: 4.393 ± 0.518
2.098AsnMet: 2.098 ± 0.266
3.868AsnAsn: 3.868 ± 0.724
2.623AsnPro: 2.623 ± 0.422
2.033AsnGln: 2.033 ± 0.454
2.295AsnArg: 2.295 ± 0.45
2.754AsnSer: 2.754 ± 0.344
3.737AsnThr: 3.737 ± 0.527
3.213AsnVal: 3.213 ± 0.559
0.59AsnTrp: 0.59 ± 0.179
1.967AsnTyr: 1.967 ± 0.341
0.0AsnXaa: 0.0 ± 0.0
Pro
2.033ProAla: 2.033 ± 0.421
0.131ProCys: 0.131 ± 0.084
1.639ProAsp: 1.639 ± 0.389
3.868ProGlu: 3.868 ± 0.556
1.377ProPhe: 1.377 ± 0.423
2.033ProGly: 2.033 ± 0.26
0.918ProHis: 0.918 ± 0.303
2.36ProIle: 2.36 ± 0.374
2.098ProLys: 2.098 ± 0.416
2.426ProLeu: 2.426 ± 0.368
1.18ProMet: 1.18 ± 0.238
1.967ProAsn: 1.967 ± 0.396
2.033ProPro: 2.033 ± 0.492
1.967ProGln: 1.967 ± 0.405
1.639ProArg: 1.639 ± 0.451
1.377ProSer: 1.377 ± 0.237
1.442ProThr: 1.442 ± 0.357
2.36ProVal: 2.36 ± 0.396
0.262ProTrp: 0.262 ± 0.134
1.246ProTyr: 1.246 ± 0.238
0.0ProXaa: 0.0 ± 0.0
Gln
2.754GlnAla: 2.754 ± 0.515
0.197GlnCys: 0.197 ± 0.112
1.77GlnAsp: 1.77 ± 0.439
2.688GlnGlu: 2.688 ± 0.362
1.574GlnPhe: 1.574 ± 0.313
2.754GlnGly: 2.754 ± 0.425
0.918GlnHis: 0.918 ± 0.234
2.819GlnIle: 2.819 ± 0.372
3.082GlnLys: 3.082 ± 0.419
2.295GlnLeu: 2.295 ± 0.333
0.983GlnMet: 0.983 ± 0.264
1.836GlnAsn: 1.836 ± 0.385
0.983GlnPro: 0.983 ± 0.286
1.901GlnGln: 1.901 ± 0.467
1.377GlnArg: 1.377 ± 0.333
1.967GlnSer: 1.967 ± 0.325
2.36GlnThr: 2.36 ± 0.45
2.098GlnVal: 2.098 ± 0.399
0.197GlnTrp: 0.197 ± 0.088
1.508GlnTyr: 1.508 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
2.426ArgAla: 2.426 ± 0.447
0.197ArgCys: 0.197 ± 0.123
2.623ArgAsp: 2.623 ± 0.477
3.934ArgGlu: 3.934 ± 0.538
1.639ArgPhe: 1.639 ± 0.426
2.229ArgGly: 2.229 ± 0.384
0.852ArgHis: 0.852 ± 0.237
3.147ArgIle: 3.147 ± 0.461
3.541ArgLys: 3.541 ± 0.563
2.95ArgLeu: 2.95 ± 0.477
1.377ArgMet: 1.377 ± 0.325
2.426ArgAsn: 2.426 ± 0.486
1.377ArgPro: 1.377 ± 0.265
1.705ArgGln: 1.705 ± 0.349
2.491ArgArg: 2.491 ± 0.524
1.574ArgSer: 1.574 ± 0.28
2.36ArgThr: 2.36 ± 0.379
3.147ArgVal: 3.147 ± 0.46
0.459ArgTrp: 0.459 ± 0.142
2.229ArgTyr: 2.229 ± 0.388
0.0ArgXaa: 0.0 ± 0.0
Ser
4.262SerAla: 4.262 ± 0.568
0.262SerCys: 0.262 ± 0.125
3.803SerAsp: 3.803 ± 0.396
4.327SerGlu: 4.327 ± 0.556
2.754SerPhe: 2.754 ± 0.44
4.917SerGly: 4.917 ± 0.862
0.656SerHis: 0.656 ± 0.22
4.327SerIle: 4.327 ± 0.51
4.655SerLys: 4.655 ± 0.522
3.803SerLeu: 3.803 ± 0.652
1.967SerMet: 1.967 ± 0.32
2.557SerAsn: 2.557 ± 0.387
1.442SerPro: 1.442 ± 0.291
1.377SerGln: 1.377 ± 0.294
2.36SerArg: 2.36 ± 0.408
2.688SerSer: 2.688 ± 0.404
3.082SerThr: 3.082 ± 0.504
3.606SerVal: 3.606 ± 0.517
0.656SerTrp: 0.656 ± 0.235
2.426SerTyr: 2.426 ± 0.406
0.0SerXaa: 0.0 ± 0.0
Thr
4.196ThrAla: 4.196 ± 0.595
0.459ThrCys: 0.459 ± 0.181
3.541ThrAsp: 3.541 ± 0.413
4.917ThrGlu: 4.917 ± 0.603
2.164ThrPhe: 2.164 ± 0.367
4.852ThrGly: 4.852 ± 0.754
1.311ThrHis: 1.311 ± 0.332
4.327ThrIle: 4.327 ± 0.623
4.458ThrLys: 4.458 ± 0.508
4.196ThrLeu: 4.196 ± 0.448
1.377ThrMet: 1.377 ± 0.302
2.688ThrAsn: 2.688 ± 0.408
2.491ThrPro: 2.491 ± 0.42
2.098ThrGln: 2.098 ± 0.272
1.639ThrArg: 1.639 ± 0.312
3.409ThrSer: 3.409 ± 0.571
4.065ThrThr: 4.065 ± 0.668
3.999ThrVal: 3.999 ± 0.493
0.525ThrTrp: 0.525 ± 0.193
1.574ThrTyr: 1.574 ± 0.328
0.0ThrXaa: 0.0 ± 0.0
Val
3.737ValAla: 3.737 ± 0.455
0.525ValCys: 0.525 ± 0.18
3.934ValAsp: 3.934 ± 0.439
3.803ValGlu: 3.803 ± 0.467
3.147ValPhe: 3.147 ± 0.428
3.213ValGly: 3.213 ± 0.611
0.918ValHis: 0.918 ± 0.246
3.672ValIle: 3.672 ± 0.434
6.622ValLys: 6.622 ± 0.711
3.803ValLeu: 3.803 ± 0.448
1.836ValMet: 1.836 ± 0.267
4.262ValAsn: 4.262 ± 0.743
1.705ValPro: 1.705 ± 0.266
3.147ValGln: 3.147 ± 0.361
2.688ValArg: 2.688 ± 0.347
4.131ValSer: 4.131 ± 0.767
3.278ValThr: 3.278 ± 0.465
3.803ValVal: 3.803 ± 0.447
0.787ValTrp: 0.787 ± 0.245
2.95ValTyr: 2.95 ± 0.473
0.0ValXaa: 0.0 ± 0.0
Trp
0.525TrpAla: 0.525 ± 0.145
0.066TrpCys: 0.066 ± 0.058
0.656TrpAsp: 0.656 ± 0.189
0.525TrpGlu: 0.525 ± 0.183
0.656TrpPhe: 0.656 ± 0.194
0.721TrpGly: 0.721 ± 0.214
0.131TrpHis: 0.131 ± 0.086
0.721TrpIle: 0.721 ± 0.21
1.311TrpLys: 1.311 ± 0.429
0.852TrpLeu: 0.852 ± 0.26
0.262TrpMet: 0.262 ± 0.124
0.787TrpAsn: 0.787 ± 0.31
0.131TrpPro: 0.131 ± 0.089
0.262TrpGln: 0.262 ± 0.126
0.459TrpArg: 0.459 ± 0.163
0.525TrpSer: 0.525 ± 0.14
0.852TrpThr: 0.852 ± 0.26
0.852TrpVal: 0.852 ± 0.236
0.066TrpTrp: 0.066 ± 0.064
0.393TrpTyr: 0.393 ± 0.226
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.295TyrAla: 2.295 ± 0.386
0.393TyrCys: 0.393 ± 0.141
2.426TyrAsp: 2.426 ± 0.432
3.213TyrGlu: 3.213 ± 0.538
1.246TyrPhe: 1.246 ± 0.233
2.688TyrGly: 2.688 ± 0.425
0.59TyrHis: 0.59 ± 0.195
2.754TyrIle: 2.754 ± 0.384
3.999TyrLys: 3.999 ± 0.681
2.885TyrLeu: 2.885 ± 0.396
1.377TyrMet: 1.377 ± 0.258
2.819TyrAsn: 2.819 ± 0.527
1.18TyrPro: 1.18 ± 0.308
1.115TyrGln: 1.115 ± 0.303
1.705TyrArg: 1.705 ± 0.372
1.967TyrSer: 1.967 ± 0.439
1.967TyrThr: 1.967 ± 0.453
2.819TyrVal: 2.819 ± 0.411
0.328TyrTrp: 0.328 ± 0.15
2.295TyrTyr: 2.295 ± 0.438
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (15253 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski