Acinetobacter phage vB_AbaM_Acibel004

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Saclayvirus; unclassified Saclayvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 156 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075DX47|A0A075DX47_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_Acibel004 OX=1481186 GN=vB_AbaM_Acibel004_44 PE=4 SV=1
MM1 pKa = 6.45YY2 pKa = 9.03TCFDD6 pKa = 3.23SWFEE10 pKa = 4.0EE11 pKa = 4.33VKK13 pKa = 10.64EE14 pKa = 4.16VADD17 pKa = 3.71EE18 pKa = 4.15CGKK21 pKa = 11.16YY22 pKa = 9.56IDD24 pKa = 4.07NASDD28 pKa = 3.52FEE30 pKa = 4.34HH31 pKa = 7.21FYY33 pKa = 11.09KK34 pKa = 10.61QGLSPDD40 pKa = 3.5DD41 pKa = 5.72AYY43 pKa = 11.51NQWILEE49 pKa = 4.28EE50 pKa = 4.43GNEE53 pKa = 3.98

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075DX51|A0A075DX51_9CAUD Dihydrofolate reductase OS=Acinetobacter phage vB_AbaM_Acibel004 OX=1481186 GN=vB_AbaM_Acibel004_30 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.02ISKK5 pKa = 9.33SLRR8 pKa = 11.84KK9 pKa = 9.82VIVYY13 pKa = 8.55KK14 pKa = 10.39TMNRR18 pKa = 11.84RR19 pKa = 11.84ISSIIKK25 pKa = 9.82DD26 pKa = 3.66LEE28 pKa = 4.39SKK30 pKa = 10.75KK31 pKa = 10.5EE32 pKa = 3.78NFYY35 pKa = 10.99FKK37 pKa = 10.75SGSQYY42 pKa = 11.38SNWTVTEE49 pKa = 4.88LKK51 pKa = 10.68AQMAINSNRR60 pKa = 11.84ILFIRR65 pKa = 11.84SKK67 pKa = 9.33MLEE70 pKa = 4.22VKK72 pKa = 10.6RR73 pKa = 11.84GTT75 pKa = 3.46

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

156

0

156

29511

40

2201

189.2

21.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.957 ± 0.335

1.267 ± 0.118

6.665 ± 0.163

7.031 ± 0.22

4.605 ± 0.269

6.672 ± 0.17

1.82 ± 0.125

6.435 ± 0.138

8.441 ± 0.175

8.116 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.175

5.286 ± 0.148

2.711 ± 0.114

3.368 ± 0.196

4.066 ± 0.185

6.452 ± 0.213

5.872 ± 0.364

7.455 ± 0.204

1.254 ± 0.084

3.829 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski