Pteropus associated gemycircularvirus 6
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTP3|A0A140CTP3_9VIRU RepA OS=Pteropus associated gemycircularvirus 6 OX=1985400 PE=3 SV=1
MM1 pKa = 7.75 PSDD4 pKa = 3.63 FHH6 pKa = 7.2 LKK8 pKa = 8.85 NRR10 pKa = 11.84 RR11 pKa = 11.84 YY12 pKa = 10.59 VLFTYY17 pKa = 10.25 SQAGPDD23 pKa = 3.36 FDD25 pKa = 3.8 YY26 pKa = 10.74 WAVVDD31 pKa = 5.11 LLGDD35 pKa = 4.15 LGAEE39 pKa = 4.22 CIIGRR44 pKa = 11.84 EE45 pKa = 3.92 EE46 pKa = 3.9 HH47 pKa = 6.94 ADD49 pKa = 3.49 GGIHH53 pKa = 5.86 FHH55 pKa = 6.37 VFTDD59 pKa = 4.01 FGRR62 pKa = 11.84 LFSTRR67 pKa = 11.84 KK68 pKa = 9.62 VRR70 pKa = 11.84 VFDD73 pKa = 4.04 VGGKK77 pKa = 9.28 HH78 pKa = 6.77 PNIQPIGRR86 pKa = 11.84 TPAKK90 pKa = 10.28 AYY92 pKa = 10.44 DD93 pKa = 3.71 YY94 pKa = 10.64 AIKK97 pKa = 10.92 DD98 pKa = 3.52 GDD100 pKa = 4.04 VVAGGLGRR108 pKa = 11.84 PGGDD112 pKa = 4.46 CDD114 pKa = 3.84 WDD116 pKa = 3.81 PDD118 pKa = 4.21 NFWSSAAHH126 pKa = 6.45 CGSADD131 pKa = 3.72 EE132 pKa = 5.03 FLHH135 pKa = 7.02 FCDD138 pKa = 4.14 QLAPRR143 pKa = 11.84 DD144 pKa = 4.14 LIRR147 pKa = 11.84 SFPNFRR153 pKa = 11.84 AYY155 pKa = 10.47 ANWKK159 pKa = 8.17 WDD161 pKa = 3.4 TGVPEE166 pKa = 3.96 YY167 pKa = 10.18 SQPVGAVFDD176 pKa = 3.84 TTAAPGIDD184 pKa = 2.86 EE185 pKa = 4.56 WLRR188 pKa = 11.84 KK189 pKa = 9.9 SLVLFGPYY197 pKa = 10.43 GCGKK201 pKa = 7.39 TVWARR206 pKa = 11.84 SLNTHH211 pKa = 6.78 IYY213 pKa = 10.3 FGSQWSGKK221 pKa = 7.77 VAFQGIEE228 pKa = 3.89 SAEE231 pKa = 3.83 YY232 pKa = 10.43 AIFDD236 pKa = 3.72 DD237 pKa = 4.09 WKK239 pKa = 10.96 GGLKK243 pKa = 10.25 GLPGYY248 pKa = 10.24 KK249 pKa = 9.7 DD250 pKa = 2.78 WFGAQWHH257 pKa = 5.05 VSMRR261 pKa = 11.84 QLHH264 pKa = 6.15 HH265 pKa = 7.33 DD266 pKa = 3.41 ARR268 pKa = 11.84 LVEE271 pKa = 4.22 WGRR274 pKa = 11.84 PIIWLCNRR282 pKa = 11.84 DD283 pKa = 3.74 PRR285 pKa = 11.84 ILSHH289 pKa = 6.61 EE290 pKa = 4.1 RR291 pKa = 11.84 DD292 pKa = 3.79 EE293 pKa = 6.02 IDD295 pKa = 3.48 WEE297 pKa = 4.18 WMEE300 pKa = 4.05 RR301 pKa = 11.84 ACDD304 pKa = 3.84 FVEE307 pKa = 4.41 VTGLLTTFRR316 pKa = 11.84 ASTEE320 pKa = 3.82
Molecular weight: 36.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.34
IPC2_protein 5.283
IPC_protein 5.296
Toseland 5.563
ProMoST 5.486
Dawson 5.461
Bjellqvist 5.537
Wikipedia 5.448
Rodwell 5.41
Grimsley 5.652
Solomon 5.461
Lehninger 5.448
Nozaki 5.677
DTASelect 5.893
Thurlkill 5.753
EMBOSS 5.728
Sillero 5.766
Patrickios 3.948
IPC_peptide 5.474
IPC2_peptide 5.779
IPC2.peptide.svr19 5.727
Protein with the highest isoelectric point:
>tr|A0A140CTP2|A0A140CTP2_9VIRU Replication-associated protein OS=Pteropus associated gemycircularvirus 6 OX=1985400 PE=3 SV=1
MM1 pKa = 8.08 AYY3 pKa = 7.92 RR4 pKa = 11.84 TLRR7 pKa = 11.84 RR8 pKa = 11.84 GRR10 pKa = 11.84 RR11 pKa = 11.84 SYY13 pKa = 8.7 TRR15 pKa = 11.84 KK16 pKa = 7.68 VTRR19 pKa = 11.84 STRR22 pKa = 11.84 RR23 pKa = 11.84 GVRR26 pKa = 11.84 TPYY29 pKa = 10.06 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 SYY34 pKa = 7.54 PTRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 YY41 pKa = 7.08 TRR43 pKa = 11.84 SGVMSRR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 VLNISSRR58 pKa = 11.84 KK59 pKa = 9.42 KK60 pKa = 9.03 RR61 pKa = 11.84 DD62 pKa = 3.34 NMVVFSNTTTEE73 pKa = 4.0 IPQGNTAYY81 pKa = 10.44 AKK83 pKa = 10.68 VPAVLNGNATYY94 pKa = 8.99 MFPWMPTARR103 pKa = 11.84 DD104 pKa = 3.29 LTLYY108 pKa = 10.51 NGTYY112 pKa = 8.15 PTIALQSARR121 pKa = 11.84 TASTCFMRR129 pKa = 11.84 GVKK132 pKa = 9.39 EE133 pKa = 3.85 ATNIRR138 pKa = 11.84 TDD140 pKa = 5.2 SGTAWQWRR148 pKa = 11.84 RR149 pKa = 11.84 IGFTLKK155 pKa = 10.54 GQRR158 pKa = 11.84 LYY160 pKa = 10.52 QANAATQWFHH170 pKa = 7.44 LEE172 pKa = 4.0 SSDD175 pKa = 3.74 GVLRR179 pKa = 11.84 VTNNVAGTPLGQSILDD195 pKa = 3.8 LVFDD199 pKa = 4.61 GQSGSDD205 pKa = 3.17 WLNLFNAKK213 pKa = 9.17 IDD215 pKa = 3.62 NDD217 pKa = 4.34 IISVKK222 pKa = 9.95 YY223 pKa = 10.71 DD224 pKa = 3.12 KK225 pKa = 10.95 TRR227 pKa = 11.84 TIASGNDD234 pKa = 2.67 RR235 pKa = 11.84 GIIRR239 pKa = 11.84 TYY241 pKa = 10.94 HH242 pKa = 6.69 DD243 pKa = 3.67 WHH245 pKa = 6.89 PMNKK249 pKa = 9.51 NLVYY253 pKa = 10.9 DD254 pKa = 4.52 DD255 pKa = 5.2 DD256 pKa = 4.49 EE257 pKa = 4.54 NAAGEE262 pKa = 4.39 RR263 pKa = 11.84 TTPWSVSGKK272 pKa = 10.17 QGMGDD277 pKa = 3.49 YY278 pKa = 10.81 YY279 pKa = 10.43 IIDD282 pKa = 4.4 FFQAATGATASDD294 pKa = 3.81 HH295 pKa = 7.26 LSFAPEE301 pKa = 3.52 ATLYY305 pKa = 9.21 WHH307 pKa = 7.08 EE308 pKa = 4.26 KK309 pKa = 9.3
Molecular weight: 35.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.838
IPC_protein 10.657
Toseland 10.379
ProMoST 10.233
Dawson 10.584
Bjellqvist 10.35
Wikipedia 10.833
Rodwell 10.73
Grimsley 10.687
Solomon 10.657
Lehninger 10.613
Nozaki 10.365
DTASelect 10.35
Thurlkill 10.438
EMBOSS 10.804
Sillero 10.511
Patrickios 10.262
IPC_peptide 10.657
IPC2_peptide 9.297
IPC2.peptide.svr19 8.491
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
884
255
320
294.7
33.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.937 ± 0.909
1.471 ± 0.429
7.919 ± 0.764
3.281 ± 0.652
5.09 ± 0.76
9.276 ± 0.779
2.715 ± 0.501
4.412 ± 0.192
3.507 ± 0.271
6.335 ± 0.255
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.357 ± 0.451
3.507 ± 0.851
5.204 ± 0.8
2.602 ± 0.252
8.597 ± 1.086
6.222 ± 0.566
6.674 ± 1.516
5.656 ± 0.177
3.281 ± 0.671
3.959 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here