Urbanus proteus nucleopolyhedrovirus
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 119 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A162GU15|A0A162GU15_9ABAC LEF-5 OS=Urbanus proteus nucleopolyhedrovirus OX=1675866 GN=lef-5 PE=4 SV=1
MM1 pKa = 7.21 TLEE4 pKa = 3.87 HH5 pKa = 7.26 DD6 pKa = 3.73 KK7 pKa = 10.73 TVRR10 pKa = 11.84 LYY12 pKa = 11.17 LCDD15 pKa = 3.66 MPDD18 pKa = 3.09 GVTNDD23 pKa = 4.3 KK24 pKa = 11.07 IEE26 pKa = 4.8 DD27 pKa = 3.74 DD28 pKa = 3.6 NVIYY32 pKa = 10.67 FEE34 pKa = 5.34 GIIEE38 pKa = 4.39 CFDD41 pKa = 5.2 DD42 pKa = 3.85 EE43 pKa = 4.82 TCNKK47 pKa = 9.81 FSFFSEE53 pKa = 4.3 LKK55 pKa = 10.4 KK56 pKa = 11.07 EE57 pKa = 3.99 EE58 pKa = 4.07 ALFMRR63 pKa = 11.84 KK64 pKa = 7.58 MFYY67 pKa = 10.91 DD68 pKa = 3.5 LLEE71 pKa = 4.24 YY72 pKa = 11.34 NNGNFCKK79 pKa = 10.51 NHH81 pKa = 5.35 VLIDD85 pKa = 3.67 ALIMYY90 pKa = 7.67 KK91 pKa = 9.83 TYY93 pKa = 11.34 AEE95 pKa = 5.35 LIDD98 pKa = 4.25 EE99 pKa = 4.54 SAFGNNILEE108 pKa = 4.35 SCVDD112 pKa = 4.48 FITNLFRR119 pKa = 11.84 IFRR122 pKa = 11.84 LNARR126 pKa = 11.84 ILVVLSNRR134 pKa = 11.84 IDD136 pKa = 3.62 YY137 pKa = 11.05 SQDD140 pKa = 3.06 NLSALLKK147 pKa = 10.53 HH148 pKa = 6.59 LSQHH152 pKa = 6.02 CLIEE156 pKa = 3.96 VCYY159 pKa = 10.89 QNDD162 pKa = 3.09
Molecular weight: 19.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.307
IPC2_protein 4.533
IPC_protein 4.457
Toseland 4.291
ProMoST 4.52
Dawson 4.418
Bjellqvist 4.596
Wikipedia 4.317
Rodwell 4.304
Grimsley 4.202
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.724
Thurlkill 4.317
EMBOSS 4.329
Sillero 4.584
Patrickios 2.765
IPC_peptide 4.418
IPC2_peptide 4.571
IPC2.peptide.svr19 4.501
Protein with the highest isoelectric point:
>tr|A0A162GUK1|A0A162GUK1_9ABAC Uncharacterized protein OS=Urbanus proteus nucleopolyhedrovirus OX=1675866 PE=4 SV=2
MM1 pKa = 7.65 INRR4 pKa = 11.84 TGFSAPRR11 pKa = 11.84 QKK13 pKa = 10.89 SEE15 pKa = 3.68 ATRR18 pKa = 11.84 DD19 pKa = 3.37 RR20 pKa = 11.84 ASQRR24 pKa = 11.84 TMRR27 pKa = 11.84 KK28 pKa = 8.94 HH29 pKa = 4.82 NRR31 pKa = 11.84 KK32 pKa = 7.25 TRR34 pKa = 11.84 ASHH37 pKa = 6.57 RR38 pKa = 11.84 NRR40 pKa = 11.84 TGFSAPRR47 pKa = 11.84 QKK49 pKa = 10.89 SEE51 pKa = 3.68 ATRR54 pKa = 11.84 DD55 pKa = 3.67 RR56 pKa = 11.84 ASKK59 pKa = 10.74 KK60 pKa = 10.65 YY61 pKa = 9.59 MFLSTVDD68 pKa = 3.2 VLIVHH73 pKa = 6.83 KK74 pKa = 10.67 SKK76 pKa = 10.74 LVQHH80 pKa = 6.59 FLYY83 pKa = 10.41 KK84 pKa = 10.34 HH85 pKa = 6.71 FYY87 pKa = 9.93 TFILFNFAMTDD98 pKa = 3.46 CSLSKK103 pKa = 10.45 INSVFCSEE111 pKa = 4.9 NLDD114 pKa = 3.92 SMM116 pKa = 4.91
Molecular weight: 13.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.853
IPC_protein 10.73
Toseland 11.023
ProMoST 10.789
Dawson 11.082
Bjellqvist 10.804
Wikipedia 11.316
Rodwell 11.316
Grimsley 11.111
Solomon 11.257
Lehninger 11.213
Nozaki 10.994
DTASelect 10.804
Thurlkill 11.008
EMBOSS 11.433
Sillero 11.023
Patrickios 11.052
IPC_peptide 11.272
IPC2_peptide 9.853
IPC2.peptide.svr19 8.53
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
119
0
119
32641
49
1187
274.3
31.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.047 ± 0.152
2.705 ± 0.16
5.818 ± 0.12
5.153 ± 0.142
5.257 ± 0.152
2.426 ± 0.127
2.482 ± 0.113
7.576 ± 0.181
7.898 ± 0.269
9.568 ± 0.221
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.629 ± 0.099
9.644 ± 0.223
2.806 ± 0.16
3.921 ± 0.21
3.934 ± 0.192
6.231 ± 0.171
5.83 ± 0.179
6.36 ± 0.137
0.631 ± 0.05
5.08 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here