Amino acid dipepetide frequency for Mycobacterium phage Tripl3t

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.964AlaAla: 12.964 ± 1.033
0.78AlaCys: 0.78 ± 0.206
6.362AlaAsp: 6.362 ± 0.646
6.002AlaGlu: 6.002 ± 0.736
3.541AlaPhe: 3.541 ± 0.445
7.682AlaGly: 7.682 ± 0.843
1.5AlaHis: 1.5 ± 0.342
4.561AlaIle: 4.561 ± 0.598
4.021AlaLys: 4.021 ± 0.565
9.363AlaLeu: 9.363 ± 0.922
2.281AlaMet: 2.281 ± 0.404
2.581AlaAsn: 2.581 ± 0.408
4.441AlaPro: 4.441 ± 0.683
3.181AlaGln: 3.181 ± 0.445
6.602AlaArg: 6.602 ± 0.645
5.402AlaSer: 5.402 ± 0.684
5.341AlaThr: 5.341 ± 0.727
7.682AlaVal: 7.682 ± 0.696
1.921AlaTrp: 1.921 ± 0.335
3.121AlaTyr: 3.121 ± 0.386
0.0AlaXaa: 0.0 ± 0.0
Cys
0.66CysAla: 0.66 ± 0.192
0.0CysCys: 0.0 ± 0.0
0.42CysAsp: 0.42 ± 0.144
0.66CysGlu: 0.66 ± 0.179
0.18CysPhe: 0.18 ± 0.106
0.6CysGly: 0.6 ± 0.21
0.12CysHis: 0.12 ± 0.097
0.18CysIle: 0.18 ± 0.103
0.3CysLys: 0.3 ± 0.129
0.48CysLeu: 0.48 ± 0.173
0.18CysMet: 0.18 ± 0.098
0.18CysAsn: 0.18 ± 0.097
0.24CysPro: 0.24 ± 0.112
0.18CysGln: 0.18 ± 0.095
0.54CysArg: 0.54 ± 0.169
0.3CysSer: 0.3 ± 0.139
0.42CysThr: 0.42 ± 0.146
0.36CysVal: 0.36 ± 0.144
0.36CysTrp: 0.36 ± 0.148
0.12CysTyr: 0.12 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
6.422AspAla: 6.422 ± 0.782
0.66AspCys: 0.66 ± 0.192
4.741AspAsp: 4.741 ± 0.514
3.721AspGlu: 3.721 ± 0.483
2.401AspPhe: 2.401 ± 0.427
6.122AspGly: 6.122 ± 0.648
1.32AspHis: 1.32 ± 0.328
2.881AspIle: 2.881 ± 0.345
2.401AspLys: 2.401 ± 0.432
6.842AspLeu: 6.842 ± 0.693
1.08AspMet: 1.08 ± 0.189
1.74AspAsn: 1.74 ± 0.326
4.621AspPro: 4.621 ± 0.594
1.801AspGln: 1.801 ± 0.35
3.661AspArg: 3.661 ± 0.465
3.361AspSer: 3.361 ± 0.417
3.841AspThr: 3.841 ± 0.359
4.321AspVal: 4.321 ± 0.521
1.56AspTrp: 1.56 ± 0.284
1.981AspTyr: 1.981 ± 0.347
0.0AspXaa: 0.0 ± 0.0
Glu
6.242GluAla: 6.242 ± 0.726
0.3GluCys: 0.3 ± 0.147
4.441GluAsp: 4.441 ± 0.519
4.981GluGlu: 4.981 ± 0.583
2.521GluPhe: 2.521 ± 0.452
4.021GluGly: 4.021 ± 0.408
1.44GluHis: 1.44 ± 0.324
3.421GluIle: 3.421 ± 0.528
2.881GluLys: 2.881 ± 0.529
7.142GluLeu: 7.142 ± 0.6
1.56GluMet: 1.56 ± 0.31
1.981GluAsn: 1.981 ± 0.385
2.581GluPro: 2.581 ± 0.405
2.521GluGln: 2.521 ± 0.412
3.541GluArg: 3.541 ± 0.567
3.721GluSer: 3.721 ± 0.49
3.901GluThr: 3.901 ± 0.553
5.402GluVal: 5.402 ± 0.622
1.62GluTrp: 1.62 ± 0.372
2.461GluTyr: 2.461 ± 0.423
0.0GluXaa: 0.0 ± 0.0
Phe
2.041PheAla: 2.041 ± 0.311
0.3PheCys: 0.3 ± 0.164
2.701PheAsp: 2.701 ± 0.296
2.461PheGlu: 2.461 ± 0.377
0.66PhePhe: 0.66 ± 0.203
3.721PheGly: 3.721 ± 0.498
0.84PheHis: 0.84 ± 0.24
1.44PheIle: 1.44 ± 0.279
1.14PheLys: 1.14 ± 0.316
2.701PheLeu: 2.701 ± 0.476
0.6PheMet: 0.6 ± 0.178
1.44PheAsn: 1.44 ± 0.265
1.921PhePro: 1.921 ± 0.337
0.78PheGln: 0.78 ± 0.235
1.68PheArg: 1.68 ± 0.33
2.281PheSer: 2.281 ± 0.441
1.74PheThr: 1.74 ± 0.311
1.921PheVal: 1.921 ± 0.359
0.54PheTrp: 0.54 ± 0.195
1.08PheTyr: 1.08 ± 0.306
0.0PheXaa: 0.0 ± 0.0
Gly
7.802GlyAla: 7.802 ± 1.103
0.54GlyCys: 0.54 ± 0.185
5.462GlyAsp: 5.462 ± 0.509
4.981GlyGlu: 4.981 ± 0.536
2.821GlyPhe: 2.821 ± 0.481
9.303GlyGly: 9.303 ± 2.439
1.801GlyHis: 1.801 ± 0.353
4.381GlyIle: 4.381 ± 0.685
3.841GlyLys: 3.841 ± 0.51
7.262GlyLeu: 7.262 ± 1.047
1.62GlyMet: 1.62 ± 0.336
3.061GlyAsn: 3.061 ± 0.394
3.361GlyPro: 3.361 ± 0.588
2.221GlyGln: 2.221 ± 0.341
5.402GlyArg: 5.402 ± 0.632
6.722GlySer: 6.722 ± 0.985
5.281GlyThr: 5.281 ± 0.73
5.402GlyVal: 5.402 ± 0.632
2.701GlyTrp: 2.701 ± 0.403
3.001GlyTyr: 3.001 ± 0.418
0.0GlyXaa: 0.0 ± 0.0
His
1.801HisAla: 1.801 ± 0.355
0.18HisCys: 0.18 ± 0.136
1.2HisAsp: 1.2 ± 0.267
1.56HisGlu: 1.56 ± 0.322
0.72HisPhe: 0.72 ± 0.247
1.2HisGly: 1.2 ± 0.298
0.72HisHis: 0.72 ± 0.199
1.02HisIle: 1.02 ± 0.237
0.96HisLys: 0.96 ± 0.295
1.38HisLeu: 1.38 ± 0.31
0.24HisMet: 0.24 ± 0.133
0.18HisAsn: 0.18 ± 0.097
1.38HisPro: 1.38 ± 0.279
0.9HisGln: 0.9 ± 0.22
1.56HisArg: 1.56 ± 0.288
0.66HisSer: 0.66 ± 0.188
1.2HisThr: 1.2 ± 0.292
1.74HisVal: 1.74 ± 0.345
0.36HisTrp: 0.36 ± 0.145
0.78HisTyr: 0.78 ± 0.241
0.0HisXaa: 0.0 ± 0.0
Ile
5.942IleAla: 5.942 ± 0.713
0.18IleCys: 0.18 ± 0.108
3.241IleAsp: 3.241 ± 0.41
3.961IleGlu: 3.961 ± 0.489
1.02IlePhe: 1.02 ± 0.236
3.781IleGly: 3.781 ± 0.505
0.84IleHis: 0.84 ± 0.238
1.861IleIle: 1.861 ± 0.34
1.74IleLys: 1.74 ± 0.407
3.481IleLeu: 3.481 ± 0.488
0.72IleMet: 0.72 ± 0.198
1.981IleAsn: 1.981 ± 0.344
3.121IlePro: 3.121 ± 0.393
1.44IleGln: 1.44 ± 0.305
2.941IleArg: 2.941 ± 0.421
3.841IleSer: 3.841 ± 0.786
3.481IleThr: 3.481 ± 0.465
3.001IleVal: 3.001 ± 0.519
0.72IleTrp: 0.72 ± 0.224
1.38IleTyr: 1.38 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
3.721LysAla: 3.721 ± 0.587
0.24LysCys: 0.24 ± 0.116
2.701LysAsp: 2.701 ± 0.491
1.981LysGlu: 1.981 ± 0.336
1.5LysPhe: 1.5 ± 0.285
2.281LysGly: 2.281 ± 0.372
1.08LysHis: 1.08 ± 0.254
1.801LysIle: 1.801 ± 0.324
1.921LysLys: 1.921 ± 0.481
3.121LysLeu: 3.121 ± 0.446
0.96LysMet: 0.96 ± 0.221
1.62LysAsn: 1.62 ± 0.278
2.881LysPro: 2.881 ± 0.442
1.32LysGln: 1.32 ± 0.266
3.001LysArg: 3.001 ± 0.426
2.881LysSer: 2.881 ± 0.498
2.521LysThr: 2.521 ± 0.383
3.541LysVal: 3.541 ± 0.473
0.9LysTrp: 0.9 ± 0.207
1.14LysTyr: 1.14 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
9.063LeuAla: 9.063 ± 0.815
0.36LeuCys: 0.36 ± 0.137
6.362LeuAsp: 6.362 ± 0.598
5.582LeuGlu: 5.582 ± 0.546
1.981LeuPhe: 1.981 ± 0.405
7.262LeuGly: 7.262 ± 0.784
1.5LeuHis: 1.5 ± 0.359
4.621LeuIle: 4.621 ± 0.578
4.081LeuLys: 4.081 ± 0.455
6.242LeuLeu: 6.242 ± 0.514
1.62LeuMet: 1.62 ± 0.303
3.541LeuAsn: 3.541 ± 0.448
5.101LeuPro: 5.101 ± 0.641
2.641LeuGln: 2.641 ± 0.35
6.302LeuArg: 6.302 ± 0.547
5.642LeuSer: 5.642 ± 0.582
6.362LeuThr: 6.362 ± 0.571
4.861LeuVal: 4.861 ± 0.702
1.14LeuTrp: 1.14 ± 0.264
2.281LeuTyr: 2.281 ± 0.366
0.0LeuXaa: 0.0 ± 0.0
Met
2.101MetAla: 2.101 ± 0.286
0.06MetCys: 0.06 ± 0.07
0.96MetAsp: 0.96 ± 0.214
1.32MetGlu: 1.32 ± 0.278
0.6MetPhe: 0.6 ± 0.181
1.62MetGly: 1.62 ± 0.269
0.42MetHis: 0.42 ± 0.153
0.72MetIle: 0.72 ± 0.243
0.96MetLys: 0.96 ± 0.24
1.14MetLeu: 1.14 ± 0.248
0.06MetMet: 0.06 ± 0.063
1.02MetAsn: 1.02 ± 0.232
0.96MetPro: 0.96 ± 0.267
0.54MetGln: 0.54 ± 0.197
1.2MetArg: 1.2 ± 0.255
1.801MetSer: 1.801 ± 0.369
1.921MetThr: 1.921 ± 0.273
1.2MetVal: 1.2 ± 0.326
0.42MetTrp: 0.42 ± 0.14
0.42MetTyr: 0.42 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
3.301AsnAla: 3.301 ± 0.448
0.06AsnCys: 0.06 ± 0.055
2.281AsnAsp: 2.281 ± 0.385
2.161AsnGlu: 2.161 ± 0.41
1.02AsnPhe: 1.02 ± 0.265
3.541AsnGly: 3.541 ± 0.595
0.66AsnHis: 0.66 ± 0.175
1.44AsnIle: 1.44 ± 0.34
0.9AsnLys: 0.9 ± 0.271
2.221AsnLeu: 2.221 ± 0.373
0.42AsnMet: 0.42 ± 0.159
0.66AsnAsn: 0.66 ± 0.16
2.401AsnPro: 2.401 ± 0.361
1.08AsnGln: 1.08 ± 0.255
1.5AsnArg: 1.5 ± 0.311
2.101AsnSer: 2.101 ± 0.415
2.101AsnThr: 2.101 ± 0.383
2.641AsnVal: 2.641 ± 0.42
0.84AsnTrp: 0.84 ± 0.212
1.26AsnTyr: 1.26 ± 0.296
0.0AsnXaa: 0.0 ± 0.0
Pro
5.221ProAla: 5.221 ± 0.547
0.48ProCys: 0.48 ± 0.192
4.381ProAsp: 4.381 ± 0.425
4.141ProGlu: 4.141 ± 0.532
2.341ProPhe: 2.341 ± 0.329
5.221ProGly: 5.221 ± 0.587
0.78ProHis: 0.78 ± 0.206
2.281ProIle: 2.281 ± 0.36
2.161ProLys: 2.161 ± 0.275
4.321ProLeu: 4.321 ± 0.532
0.66ProMet: 0.66 ± 0.224
1.5ProAsn: 1.5 ± 0.321
2.941ProPro: 2.941 ± 0.44
1.44ProGln: 1.44 ± 0.317
2.521ProArg: 2.521 ± 0.449
3.241ProSer: 3.241 ± 0.423
4.201ProThr: 4.201 ± 0.435
3.661ProVal: 3.661 ± 0.471
0.96ProTrp: 0.96 ± 0.323
1.68ProTyr: 1.68 ± 0.342
0.0ProXaa: 0.0 ± 0.0
Gln
3.301GlnAla: 3.301 ± 0.424
0.12GlnCys: 0.12 ± 0.077
1.08GlnAsp: 1.08 ± 0.3
1.801GlnGlu: 1.801 ± 0.298
1.2GlnPhe: 1.2 ± 0.322
2.521GlnGly: 2.521 ± 0.352
0.54GlnHis: 0.54 ± 0.15
2.461GlnIle: 2.461 ± 0.473
0.9GlnLys: 0.9 ± 0.202
3.841GlnLeu: 3.841 ± 0.47
0.96GlnMet: 0.96 ± 0.238
0.54GlnAsn: 0.54 ± 0.141
2.041GlnPro: 2.041 ± 0.391
1.68GlnGln: 1.68 ± 0.38
1.921GlnArg: 1.921 ± 0.452
1.74GlnSer: 1.74 ± 0.336
1.5GlnThr: 1.5 ± 0.305
2.161GlnVal: 2.161 ± 0.349
0.6GlnTrp: 0.6 ± 0.159
0.42GlnTyr: 0.42 ± 0.15
0.0GlnXaa: 0.0 ± 0.0
Arg
5.762ArgAla: 5.762 ± 0.685
0.78ArgCys: 0.78 ± 0.219
3.001ArgAsp: 3.001 ± 0.467
4.381ArgGlu: 4.381 ± 0.556
1.68ArgPhe: 1.68 ± 0.347
5.221ArgGly: 5.221 ± 0.654
1.26ArgHis: 1.26 ± 0.277
3.601ArgIle: 3.601 ± 0.497
3.301ArgLys: 3.301 ± 0.527
5.942ArgLeu: 5.942 ± 0.701
1.801ArgMet: 1.801 ± 0.299
2.161ArgAsn: 2.161 ± 0.406
2.401ArgPro: 2.401 ± 0.367
2.161ArgGln: 2.161 ± 0.412
4.861ArgArg: 4.861 ± 0.648
3.781ArgSer: 3.781 ± 0.519
3.121ArgThr: 3.121 ± 0.403
5.101ArgVal: 5.101 ± 0.502
1.26ArgTrp: 1.26 ± 0.299
1.68ArgTyr: 1.68 ± 0.288
0.0ArgXaa: 0.0 ± 0.0
Ser
6.662SerAla: 6.662 ± 0.919
0.48SerCys: 0.48 ± 0.165
3.481SerAsp: 3.481 ± 0.461
3.601SerGlu: 3.601 ± 0.505
1.981SerPhe: 1.981 ± 0.352
7.322SerGly: 7.322 ± 1.062
1.26SerHis: 1.26 ± 0.307
2.881SerIle: 2.881 ± 0.558
2.221SerLys: 2.221 ± 0.339
5.281SerLeu: 5.281 ± 0.579
1.38SerMet: 1.38 ± 0.313
2.281SerAsn: 2.281 ± 0.401
3.361SerPro: 3.361 ± 0.436
1.981SerGln: 1.981 ± 0.298
3.061SerArg: 3.061 ± 0.364
3.601SerSer: 3.601 ± 0.811
3.001SerThr: 3.001 ± 0.457
4.501SerVal: 4.501 ± 0.591
1.32SerTrp: 1.32 ± 0.31
1.32SerTyr: 1.32 ± 0.323
0.0SerXaa: 0.0 ± 0.0
Thr
6.002ThrAla: 6.002 ± 0.677
0.12ThrCys: 0.12 ± 0.088
4.621ThrAsp: 4.621 ± 0.559
4.801ThrGlu: 4.801 ± 0.534
2.041ThrPhe: 2.041 ± 0.378
6.242ThrGly: 6.242 ± 0.62
0.96ThrHis: 0.96 ± 0.287
2.521ThrIle: 2.521 ± 0.593
2.461ThrLys: 2.461 ± 0.336
5.942ThrLeu: 5.942 ± 0.612
1.02ThrMet: 1.02 ± 0.253
1.981ThrAsn: 1.981 ± 0.346
3.721ThrPro: 3.721 ± 0.46
1.68ThrGln: 1.68 ± 0.325
3.481ThrArg: 3.481 ± 0.497
3.181ThrSer: 3.181 ± 0.506
4.681ThrThr: 4.681 ± 0.623
5.522ThrVal: 5.522 ± 0.588
1.26ThrTrp: 1.26 ± 0.311
1.5ThrTyr: 1.5 ± 0.314
0.0ThrXaa: 0.0 ± 0.0
Val
6.602ValAla: 6.602 ± 0.632
0.36ValCys: 0.36 ± 0.14
5.402ValAsp: 5.402 ± 0.517
4.801ValGlu: 4.801 ± 0.52
2.401ValPhe: 2.401 ± 0.345
4.741ValGly: 4.741 ± 0.689
1.62ValHis: 1.62 ± 0.245
3.601ValIle: 3.601 ± 0.406
3.061ValLys: 3.061 ± 0.463
5.762ValLeu: 5.762 ± 0.727
1.14ValMet: 1.14 ± 0.289
2.521ValAsn: 2.521 ± 0.363
4.441ValPro: 4.441 ± 0.595
1.801ValGln: 1.801 ± 0.364
5.221ValArg: 5.221 ± 0.722
4.861ValSer: 4.861 ± 0.492
5.341ValThr: 5.341 ± 0.514
5.041ValVal: 5.041 ± 0.563
1.14ValTrp: 1.14 ± 0.242
2.281ValTyr: 2.281 ± 0.394
0.0ValXaa: 0.0 ± 0.0
Trp
1.32TrpAla: 1.32 ± 0.338
0.3TrpCys: 0.3 ± 0.132
1.56TrpAsp: 1.56 ± 0.261
1.08TrpGlu: 1.08 ± 0.234
0.72TrpPhe: 0.72 ± 0.212
1.921TrpGly: 1.921 ± 0.332
0.66TrpHis: 0.66 ± 0.193
1.26TrpIle: 1.26 ± 0.245
0.48TrpLys: 0.48 ± 0.241
1.74TrpLeu: 1.74 ± 0.323
0.48TrpMet: 0.48 ± 0.193
0.48TrpAsn: 0.48 ± 0.186
0.84TrpPro: 0.84 ± 0.237
0.84TrpGln: 0.84 ± 0.203
1.5TrpArg: 1.5 ± 0.35
0.78TrpSer: 0.78 ± 0.209
1.861TrpThr: 1.861 ± 0.388
2.101TrpVal: 2.101 ± 0.298
0.54TrpTrp: 0.54 ± 0.192
0.24TrpTyr: 0.24 ± 0.105
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.401TyrAla: 2.401 ± 0.395
0.18TyrCys: 0.18 ± 0.093
1.08TyrAsp: 1.08 ± 0.273
2.701TyrGlu: 2.701 ± 0.386
0.66TyrPhe: 0.66 ± 0.178
2.641TyrGly: 2.641 ± 0.431
0.42TyrHis: 0.42 ± 0.157
1.74TyrIle: 1.74 ± 0.326
1.38TyrLys: 1.38 ± 0.298
2.341TyrLeu: 2.341 ± 0.382
0.6TyrMet: 0.6 ± 0.169
1.14TyrAsn: 1.14 ± 0.304
1.38TyrPro: 1.38 ± 0.256
1.14TyrGln: 1.14 ± 0.264
2.761TyrArg: 2.761 ± 0.427
1.14TyrSer: 1.14 ± 0.238
1.921TyrThr: 1.921 ± 0.346
1.981TyrVal: 1.981 ± 0.317
0.54TyrTrp: 0.54 ± 0.179
0.54TyrTyr: 0.54 ± 0.183
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (16663 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski