Microbacterium phage TinyTimothy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Eekayvirinae; Tinytimothyvirus; Microbacterium virus TinyTimothy

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EPV3|A0A4Y6EPV3_9CAUD Uncharacterized protein OS=Microbacterium phage TinyTimothy OX=2583039 GN=51 PE=4 SV=1
MM1 pKa = 8.25DD2 pKa = 5.47DD3 pKa = 4.88AIHH6 pKa = 6.99LFVQEE11 pKa = 4.28TAGEE15 pKa = 4.08NAPALHH21 pKa = 6.3GWALAFEE28 pKa = 4.7TSRR31 pKa = 11.84LEE33 pKa = 3.91PDD35 pKa = 3.42VEE37 pKa = 4.35LFPLATAQNYY47 pKa = 9.87SFGPQTTPSQAIGLYY62 pKa = 9.44RR63 pKa = 11.84YY64 pKa = 10.05AGTVLEE70 pKa = 4.76TYY72 pKa = 10.31LVNQTLGLSS81 pKa = 3.64

Molecular weight: 8.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EE82|A0A4Y6EE82_9CAUD Uncharacterized protein OS=Microbacterium phage TinyTimothy OX=2583039 GN=45 PE=4 SV=1
MM1 pKa = 7.82PLNRR5 pKa = 11.84HH6 pKa = 4.21KK7 pKa = 9.69TIRR10 pKa = 11.84QALSYY15 pKa = 10.95VDD17 pKa = 5.75RR18 pKa = 11.84NPDD21 pKa = 2.72WPEE24 pKa = 3.4IQRR27 pKa = 11.84LDD29 pKa = 3.48MPIWEE34 pKa = 5.03LISRR38 pKa = 11.84NLFDD42 pKa = 5.1IANHH46 pKa = 6.68PNTKK50 pKa = 9.78VRR52 pKa = 11.84GSLARR57 pKa = 11.84ATRR60 pKa = 11.84AQRR63 pKa = 11.84ILLDD67 pKa = 3.55RR68 pKa = 11.84LTGTRR73 pKa = 11.84RR74 pKa = 11.84QGTNPAVRR82 pKa = 11.84NSKK85 pKa = 10.16SLQLLDD91 pKa = 3.54LTKK94 pKa = 10.95DD95 pKa = 3.28LTEE98 pKa = 4.37GQTNGEE104 pKa = 3.95DD105 pKa = 3.4SARR108 pKa = 11.84EE109 pKa = 3.97VGG111 pKa = 3.95

Molecular weight: 12.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

16995

43

4489

320.7

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.244 ± 0.357

0.435 ± 0.144

6.525 ± 0.193

6.773 ± 0.358

3.301 ± 0.153

7.502 ± 0.295

1.524 ± 0.188

4.854 ± 0.178

4.331 ± 0.371

8.585 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.149

4.725 ± 0.298

4.79 ± 0.201

4.437 ± 0.243

6.084 ± 0.426

6.325 ± 0.231

6.761 ± 0.297

6.361 ± 0.256

1.671 ± 0.134

3.254 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski