Capybara microvirus Cap1_SP_64
Average proteome isoelectric point is 7.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7J2|A0A4P8W7J2_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_64 OX=2584787 PE=4 SV=1
MM1 pKa = 7.63 AIEE4 pKa = 4.05 NSFVYY9 pKa = 10.62 SLTPVDD15 pKa = 3.31 QTDD18 pKa = 3.56 YY19 pKa = 11.43 NKK21 pKa = 10.22 FDD23 pKa = 3.8 FGYY26 pKa = 10.87 GKK28 pKa = 10.64 YY29 pKa = 8.31 FTAKK33 pKa = 9.58 PGKK36 pKa = 9.68 FYY38 pKa = 10.23 PLHH41 pKa = 5.91 WEE43 pKa = 4.22 EE44 pKa = 4.88 IEE46 pKa = 4.23 PGANIHH52 pKa = 6.93 SKK54 pKa = 9.13 IQHH57 pKa = 6.04 FARR60 pKa = 11.84 TQPLVAPSMIDD71 pKa = 3.23 MNIEE75 pKa = 3.98 TFSFLVPFRR84 pKa = 11.84 IIFDD88 pKa = 3.76 GWQEE92 pKa = 4.11 FISFGDD98 pKa = 3.56 GKK100 pKa = 8.06 TLKK103 pKa = 10.25 NDD105 pKa = 3.74 LGINSQIPYY114 pKa = 8.78 ITTQQLLQYY123 pKa = 9.17 FAHH126 pKa = 7.33 DD127 pKa = 5.35 LSCPWNLGFKK137 pKa = 10.01 PDD139 pKa = 3.23 NCNYY143 pKa = 9.49 YY144 pKa = 8.69 VTYY147 pKa = 10.47 KK148 pKa = 10.77 DD149 pKa = 5.65 LYY151 pKa = 9.21 QCCEE155 pKa = 4.04 LLDD158 pKa = 3.92 IPMSNLIDD166 pKa = 3.44 IDD168 pKa = 3.93 EE169 pKa = 4.55 FQSYY173 pKa = 8.2 DD174 pKa = 3.62 TIKK177 pKa = 10.18 TFILQTFTHH186 pKa = 7.05 FSLTDD191 pKa = 3.13 TGQKK195 pKa = 8.81 FTIDD199 pKa = 3.35 QLFTSQYY206 pKa = 9.64 VQIFDD211 pKa = 4.72 DD212 pKa = 4.57 LNIHH216 pKa = 6.24 TFPVNPQNFATLHH229 pKa = 5.86 QITNSASTNLAFPKK243 pKa = 10.59 YY244 pKa = 9.47 SFSKK248 pKa = 10.54 KK249 pKa = 10.09 SIWYY253 pKa = 8.33 NALNLYY259 pKa = 9.51 HH260 pKa = 7.11 DD261 pKa = 4.78 KK262 pKa = 10.71 EE263 pKa = 4.56 YY264 pKa = 10.9 EE265 pKa = 4.07 SHH267 pKa = 7.14 DD268 pKa = 3.48 TQLYY272 pKa = 8.26 YY273 pKa = 10.85 DD274 pKa = 3.82 NKK276 pKa = 8.6 EE277 pKa = 4.34 TYY279 pKa = 7.69 DD280 pKa = 3.66 TSSSSLWRR288 pKa = 11.84 TRR290 pKa = 11.84 LLNKK294 pKa = 10.45 LEE296 pKa = 4.27 TNNIRR301 pKa = 11.84 SYY303 pKa = 10.18 WLYY306 pKa = 11.17 KK307 pKa = 10.77 EE308 pKa = 5.64 GDD310 pKa = 4.02 TKK312 pKa = 11.01 PSALWFATTPQIKK325 pKa = 8.37 TLNNSFKK332 pKa = 10.59 FAQSKK337 pKa = 10.69 INLLPFFAKK346 pKa = 10.16 QKK348 pKa = 10.63 VYY350 pKa = 10.83 EE351 pKa = 4.09 DD352 pKa = 3.43 WFRR355 pKa = 11.84 CEE357 pKa = 3.97 PFEE360 pKa = 4.43 TEE362 pKa = 3.84 YY363 pKa = 11.26 SEE365 pKa = 4.01 YY366 pKa = 10.5 TIFSGKK372 pKa = 7.53 VTEE375 pKa = 4.76 LPTSEE380 pKa = 4.11 FVKK383 pKa = 10.67 VLHH386 pKa = 6.76 LSPAHH391 pKa = 5.58 YY392 pKa = 10.25 KK393 pKa = 9.97 RR394 pKa = 11.84 DD395 pKa = 3.69 YY396 pKa = 10.48 FQAALPQPTALQQVFVPFSSPIDD419 pKa = 3.52 LPGISNPFSDD429 pKa = 4.28 NRR431 pKa = 11.84 PVTLQMNSDD440 pKa = 3.15 KK441 pKa = 11.13 TLFAEE446 pKa = 4.55 YY447 pKa = 10.23 KK448 pKa = 9.7 PDD450 pKa = 3.57 PTRR453 pKa = 11.84 STIAEE458 pKa = 3.96 RR459 pKa = 11.84 VEE461 pKa = 3.75 NLGDD465 pKa = 5.09 HH466 pKa = 6.67 ILTYY470 pKa = 10.98 DD471 pKa = 3.57 NEE473 pKa = 4.95 SNPSYY478 pKa = 10.13 LTVNSIRR485 pKa = 11.84 EE486 pKa = 4.13 SFALQDD492 pKa = 3.68 YY493 pKa = 11.14 LEE495 pKa = 4.39 TLNAVGNRR503 pKa = 11.84 YY504 pKa = 9.51 VEE506 pKa = 4.17 MMYY509 pKa = 11.23 SMYY512 pKa = 10.64 GVQVPDD518 pKa = 3.58 AVVKK522 pKa = 10.58 RR523 pKa = 11.84 PLYY526 pKa = 10.45 LNHH529 pKa = 7.48 DD530 pKa = 3.9 SDD532 pKa = 3.88 ILKK535 pKa = 10.5 ISDD538 pKa = 3.76 VEE540 pKa = 4.8 SNSQTLLNNNEE551 pKa = 3.93 INTPLGTLAGKK562 pKa = 10.31 GIAYY566 pKa = 7.78 STGSGYY572 pKa = 10.8 NIQASEE578 pKa = 4.22 FCILISFMRR587 pKa = 11.84 ISPPSIYY594 pKa = 10.41 FQGLPRR600 pKa = 11.84 KK601 pKa = 9.32 FMHH604 pKa = 7.02 FDD606 pKa = 3.35 PLDD609 pKa = 4.05 FYY611 pKa = 11.01 NHH613 pKa = 6.15 KK614 pKa = 9.84 FAHH617 pKa = 6.49 LGEE620 pKa = 4.36 QEE622 pKa = 4.26 TFQGEE627 pKa = 4.31 IYY629 pKa = 9.33 FTGKK633 pKa = 10.02 QDD635 pKa = 4.02 DD636 pKa = 3.85 DD637 pKa = 4.82 SKK639 pKa = 11.71 VFGYY643 pKa = 8.75 MPRR646 pKa = 11.84 FSEE649 pKa = 3.9 YY650 pKa = 9.35 RR651 pKa = 11.84 GQVSRR656 pKa = 11.84 SCGEE660 pKa = 4.06 LNTNLHH666 pKa = 5.26 YY667 pKa = 10.25 WLPKK671 pKa = 10.0 RR672 pKa = 11.84 IFNGAPKK679 pKa = 10.22 LGSNFSMQHH688 pKa = 5.83 PNDD691 pKa = 4.45 TIDD694 pKa = 4.92 MFTNWDD700 pKa = 3.44 QNYY703 pKa = 10.59 DD704 pKa = 3.82 SIIFQSYY711 pKa = 9.67 IKK713 pKa = 9.49 IDD715 pKa = 3.31 KK716 pKa = 10.17 SSRR719 pKa = 11.84 IPVYY723 pKa = 9.67 PIPSHH728 pKa = 6.34 LCIYY732 pKa = 10.37 NHH734 pKa = 6.86
Molecular weight: 85.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.097
IPC2_protein 5.207
IPC_protein 5.219
Toseland 5.385
ProMoST 5.423
Dawson 5.334
Bjellqvist 5.423
Wikipedia 5.27
Rodwell 5.27
Grimsley 5.423
Solomon 5.334
Lehninger 5.308
Nozaki 5.512
DTASelect 5.703
Thurlkill 5.486
EMBOSS 5.461
Sillero 5.601
Patrickios 3.096
IPC_peptide 5.347
IPC2_peptide 5.601
IPC2.peptide.svr19 5.496
Protein with the highest isoelectric point:
>tr|A0A4V1FVM9|A0A4V1FVM9_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_64 OX=2584787 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 9.85 CLHH5 pKa = 6.42 PVGVYY10 pKa = 9.87 INKK13 pKa = 9.79 NYY15 pKa = 10.17 KK16 pKa = 10.17 DD17 pKa = 3.55 LGFFQVPCGKK27 pKa = 10.4 CYY29 pKa = 10.49 NCKK32 pKa = 9.22 QNKK35 pKa = 8.39 AKK37 pKa = 10.5 SVAFRR42 pKa = 11.84 LEE44 pKa = 4.43 LEE46 pKa = 4.23 TQKK49 pKa = 11.23 ASNSFFVTLTYY60 pKa = 10.02 PDD62 pKa = 3.64 HH63 pKa = 7.26 SINKK67 pKa = 8.8 INGVSCVDD75 pKa = 3.15 NFITHH80 pKa = 7.56 RR81 pKa = 11.84 IFKK84 pKa = 10.24 RR85 pKa = 11.84 LRR87 pKa = 11.84 SQVWNEE93 pKa = 3.67 IKK95 pKa = 10.56 KK96 pKa = 10.31 SLHH99 pKa = 6.16 KK100 pKa = 10.61 LPCNRR105 pKa = 11.84 FSPPSVKK112 pKa = 9.79 QRR114 pKa = 11.84 NRR116 pKa = 11.84 QISALRR122 pKa = 11.84 RR123 pKa = 11.84 HH124 pKa = 6.5 KK125 pKa = 11.0 YY126 pKa = 10.22 SLDD129 pKa = 3.92 PIVRR133 pKa = 11.84 SKK135 pKa = 11.4 YY136 pKa = 10.58 FLISEE141 pKa = 4.35 YY142 pKa = 11.22 GPLTSRR148 pKa = 11.84 PHH150 pKa = 4.86 YY151 pKa = 9.52 HH152 pKa = 6.92 FIWFFYY158 pKa = 10.71 DD159 pKa = 3.15 QVDD162 pKa = 3.63 VMEE165 pKa = 4.52 VEE167 pKa = 5.14 KK168 pKa = 11.01 KK169 pKa = 10.83 LLDD172 pKa = 3.52 LWDD175 pKa = 4.88 GIITVDD181 pKa = 4.18 LVNDD185 pKa = 3.24 ARR187 pKa = 11.84 INYY190 pKa = 7.88 VSQYY194 pKa = 10.25 CNKK197 pKa = 10.18 DD198 pKa = 3.35 CEE200 pKa = 4.38 PQLFGQSKK208 pKa = 10.41 NITRR212 pKa = 11.84 CSINIGASLEE222 pKa = 3.92 NSEE225 pKa = 4.28 NYY227 pKa = 9.92 NRR229 pKa = 11.84 ILSQQCPRR237 pKa = 11.84 VSWRR241 pKa = 11.84 KK242 pKa = 10.03 GSIKK246 pKa = 10.43 LPSYY250 pKa = 9.75 YY251 pKa = 9.45 IRR253 pKa = 11.84 RR254 pKa = 11.84 AQKK257 pKa = 10.13 HH258 pKa = 5.63 GKK260 pKa = 8.8 LQIKK264 pKa = 10.06 DD265 pKa = 3.48 SYY267 pKa = 11.29 EE268 pKa = 4.13 LYY270 pKa = 10.33 TEE272 pKa = 4.38 SKK274 pKa = 10.15 KK275 pKa = 9.75 QQQEE279 pKa = 3.36 IDD281 pKa = 3.9 HH282 pKa = 6.74 KK283 pKa = 10.98 IKK285 pKa = 10.08 MYY287 pKa = 9.36 CRR289 pKa = 11.84 KK290 pKa = 10.11 YY291 pKa = 10.64 KK292 pKa = 10.28 ILNKK296 pKa = 10.31 HH297 pKa = 5.73 EE298 pKa = 4.05 FLKK301 pKa = 11.02 FKK303 pKa = 10.44 ISYY306 pKa = 9.86 IYY308 pKa = 10.96 ANEE311 pKa = 3.7 KK312 pKa = 9.87 NKK314 pKa = 10.31 RR315 pKa = 11.84 NKK317 pKa = 9.53 RR318 pKa = 11.84 ANII321 pKa = 3.63
Molecular weight: 38.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.172
IPC2_protein 9.209
IPC_protein 9.092
Toseland 9.809
ProMoST 9.531
Dawson 10.043
Bjellqvist 9.75
Wikipedia 10.204
Rodwell 10.467
Grimsley 10.116
Solomon 10.058
Lehninger 10.014
Nozaki 9.897
DTASelect 9.721
Thurlkill 9.897
EMBOSS 10.218
Sillero 9.97
Patrickios 7.541
IPC_peptide 10.058
IPC2_peptide 8.507
IPC2.peptide.svr19 8.202
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1645
79
734
274.2
31.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.83 ± 0.96
1.216 ± 0.556
5.775 ± 0.534
4.559 ± 0.341
6.444 ± 1.285
3.1 ± 0.705
2.553 ± 0.249
7.416 ± 0.432
7.599 ± 1.145
8.997 ± 0.776
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.337 ± 0.293
8.085 ± 0.908
4.316 ± 0.833
6.14 ± 1.189
3.587 ± 0.821
9.119 ± 0.696
5.775 ± 0.858
4.134 ± 0.608
0.851 ± 0.34
5.167 ± 0.948
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here