Massariosphaeria phaeospora

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporales incertae sedis; Massariosphaeria

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14012 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C8I250|A0A7C8I250_9PLEO Histidine biosynthesis trifunctional protein OS=Massariosphaeria phaeospora OX=100035 GN=BDV95DRAFT_525914 PE=3 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84EE4 pKa = 4.48CVSCLSPEE12 pKa = 4.19EE13 pKa = 4.22EE14 pKa = 4.4CPLRR18 pKa = 11.84QTPCGDD24 pKa = 3.44HH25 pKa = 6.13YY26 pKa = 11.73LCDD29 pKa = 3.59EE30 pKa = 4.54CLEE33 pKa = 4.08QLFTLALNDD42 pKa = 3.66EE43 pKa = 4.25KK44 pKa = 10.83HH45 pKa = 5.71YY46 pKa = 10.58PPRR49 pKa = 11.84CCRR52 pKa = 11.84HH53 pKa = 5.09QSSLLLIGEE62 pKa = 4.77FEE64 pKa = 4.15QHH66 pKa = 6.61LPAQLMVEE74 pKa = 4.23YY75 pKa = 9.88QAKK78 pKa = 10.3EE79 pKa = 3.77NGEE82 pKa = 4.17YY83 pKa = 10.47AVQPRR88 pKa = 11.84FRR90 pKa = 11.84RR91 pKa = 11.84YY92 pKa = 9.86CADD95 pKa = 3.65PSCAKK100 pKa = 10.19FLRR103 pKa = 11.84PASHH107 pKa = 6.43ITDD110 pKa = 3.02SDD112 pKa = 3.75MNITYY117 pKa = 9.46AICEE121 pKa = 3.84ADD123 pKa = 3.44KK124 pKa = 10.92CLKK127 pKa = 8.79ATCIDD132 pKa = 3.93CRR134 pKa = 11.84TLLLNGTADD143 pKa = 3.81HH144 pKa = 6.16VCEE147 pKa = 5.19VPDD150 pKa = 3.5SDD152 pKa = 5.28KK153 pKa = 11.25KK154 pKa = 10.92FKK156 pKa = 9.58EE157 pKa = 3.95TAKK160 pKa = 10.42EE161 pKa = 4.0KK162 pKa = 10.61GYY164 pKa = 10.1QSCFACGSTVEE175 pKa = 4.2LAEE178 pKa = 4.3ACNHH182 pKa = 5.11ITCGCGNNFCYY193 pKa = 10.75VCGKK197 pKa = 8.92EE198 pKa = 3.83WPGMHH203 pKa = 7.14GCPQYY208 pKa = 11.39GPAVYY213 pKa = 10.31DD214 pKa = 3.57EE215 pKa = 4.68EE216 pKa = 6.46GYY218 pKa = 10.22NQDD221 pKa = 4.72GYY223 pKa = 11.63HH224 pKa = 7.11KK225 pKa = 10.13DD226 pKa = 2.99TGLNRR231 pKa = 11.84DD232 pKa = 3.07GLTRR236 pKa = 11.84VQVHH240 pKa = 5.84IRR242 pKa = 11.84DD243 pKa = 3.95GADD246 pKa = 3.65DD247 pKa = 6.15DD248 pKa = 5.99DD249 pKa = 7.39DD250 pKa = 5.72EE251 pKa = 7.9DD252 pKa = 6.76DD253 pKa = 5.76DD254 pKa = 4.86VDD256 pKa = 4.41ANRR259 pKa = 11.84DD260 pKa = 3.54EE261 pKa = 6.33DD262 pKa = 5.01DD263 pKa = 4.06EE264 pKa = 5.66DD265 pKa = 3.57WDD267 pKa = 4.36EE268 pKa = 5.07EE269 pKa = 4.57VLQHH273 pKa = 7.49LDD275 pKa = 3.18DD276 pKa = 4.93DD277 pKa = 4.09QRR279 pKa = 11.84AMINNMDD286 pKa = 3.75PVLRR290 pKa = 11.84EE291 pKa = 3.48EE292 pKa = 4.07TLEE295 pKa = 4.01VFRR298 pKa = 11.84VQLFEE303 pKa = 3.79EE304 pKa = 4.42RR305 pKa = 11.84GIIFQQGDD313 pKa = 3.66GHH315 pKa = 6.26HH316 pKa = 6.4HH317 pKa = 6.12HH318 pKa = 6.43QHH320 pKa = 5.55GQDD323 pKa = 3.38LARR326 pKa = 11.84EE327 pKa = 4.2DD328 pKa = 5.45DD329 pKa = 4.69ADD331 pKa = 6.19DD332 pKa = 6.01DD333 pKa = 6.27DD334 pKa = 6.76ADD336 pKa = 3.66QDD338 pKa = 4.03NPSDD342 pKa = 4.21EE343 pKa = 5.8DD344 pKa = 5.28DD345 pKa = 6.23GDD347 pKa = 4.85DD348 pKa = 5.25QDD350 pKa = 6.33DD351 pKa = 4.65DD352 pKa = 5.01QDD354 pKa = 5.42DD355 pKa = 4.62DD356 pKa = 5.54GNDD359 pKa = 4.21DD360 pKa = 4.02EE361 pKa = 6.72LEE363 pKa = 4.54DD364 pKa = 3.89PTDD367 pKa = 3.32EE368 pKa = 5.52RR369 pKa = 11.84FADD372 pKa = 3.97DD373 pKa = 3.62QSADD377 pKa = 3.49EE378 pKa = 4.84ALFDD382 pKa = 4.53IDD384 pKa = 5.98DD385 pKa = 3.84IVASVEE391 pKa = 4.28DD392 pKa = 3.92VLATADD398 pKa = 4.39AINDD402 pKa = 3.36IVSGVEE408 pKa = 3.52AGLATTSQDD417 pKa = 5.1DD418 pKa = 4.2EE419 pKa = 5.55IEE421 pKa = 4.58DD422 pKa = 3.59EE423 pKa = 4.54GDD425 pKa = 3.71ALVHH429 pKa = 6.27SPADD433 pKa = 3.8SAILPALSANEE444 pKa = 3.79DD445 pKa = 4.15DD446 pKa = 4.39AWSSNSPVSPPQQDD460 pKa = 3.2LTEE463 pKa = 3.99QSIEE467 pKa = 4.12PKK469 pKa = 10.23TPGDD473 pKa = 3.64VMQLSDD479 pKa = 4.75AAYY482 pKa = 10.4GDD484 pKa = 4.47DD485 pKa = 4.46NGGAGAGPMYY495 pKa = 10.76GGGLTGWGGGEE506 pKa = 4.0EE507 pKa = 4.17LL508 pKa = 4.88

Molecular weight: 56.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C8I4H0|A0A7C8I4H0_9PLEO Uncharacterized protein OS=Massariosphaeria phaeospora OX=100035 GN=BDV95DRAFT_167222 PE=4 SV=1
MM1 pKa = 7.23NALRR5 pKa = 11.84QHH7 pKa = 5.91SNRR10 pKa = 11.84PQSHH14 pKa = 6.05KK15 pKa = 10.84SFRR18 pKa = 11.84TKK20 pKa = 10.44QKK22 pKa = 10.05LARR25 pKa = 11.84AQKK28 pKa = 8.97QNRR31 pKa = 11.84PIPQWIRR38 pKa = 11.84LRR40 pKa = 11.84TNNTIRR46 pKa = 11.84YY47 pKa = 5.73NAKK50 pKa = 8.89RR51 pKa = 11.84RR52 pKa = 11.84HH53 pKa = 4.14WRR55 pKa = 11.84KK56 pKa = 7.41TRR58 pKa = 11.84LGII61 pKa = 4.46

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14012

0

14012

6065376

49

7228

432.9

47.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.882 ± 0.019

1.322 ± 0.01

5.619 ± 0.016

6.003 ± 0.019

3.647 ± 0.013

6.773 ± 0.018

2.563 ± 0.01

4.62 ± 0.014

4.773 ± 0.021

8.893 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.154 ± 0.007

3.546 ± 0.013

6.335 ± 0.024

3.996 ± 0.013

6.358 ± 0.021

8.039 ± 0.024

6.114 ± 0.013

6.123 ± 0.014

1.499 ± 0.008

2.742 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski