Prevotella dentalis (strain ATCC 49559 / DSM 3688 / JCM 13448 / NCTC 12043 / ES 2772) (Mitsuokella dentalis)
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2494 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9D760|F9D760_PREDD ATPase OS=Prevotella dentalis (strain ATCC 49559 / DSM 3688 / JCM 13448 / NCTC 12043 / ES 2772) OX=908937 GN=Prede_2529 PE=4 SV=1
MM1 pKa = 7.63 EE2 pKa = 4.62 ATDD5 pKa = 4.17 LNEE8 pKa = 3.23 ARR10 pKa = 11.84 IYY12 pKa = 10.4 VGTYY16 pKa = 9.66 AKK18 pKa = 10.48 YY19 pKa = 11.08 NNGSLQGEE27 pKa = 4.35 WVEE30 pKa = 5.59 LSDD33 pKa = 6.54 FYY35 pKa = 11.8 DD36 pKa = 3.73 LDD38 pKa = 3.98 GFMEE42 pKa = 4.68 RR43 pKa = 11.84 CAGIHH48 pKa = 5.94 EE49 pKa = 4.96 DD50 pKa = 3.93 EE51 pKa = 5.0 EE52 pKa = 4.46 EE53 pKa = 4.28 PEE55 pKa = 4.24 YY56 pKa = 10.63 MFQAWEE62 pKa = 4.46 EE63 pKa = 4.25 IPDD66 pKa = 3.7 GLIAEE71 pKa = 4.52 GHH73 pKa = 5.97 LEE75 pKa = 3.9 EE76 pKa = 4.97 TFFEE80 pKa = 5.11 LRR82 pKa = 11.84 DD83 pKa = 3.54 EE84 pKa = 4.57 LDD86 pKa = 3.4 RR87 pKa = 11.84 LNDD90 pKa = 3.7 TEE92 pKa = 6.27 KK93 pKa = 10.62 EE94 pKa = 4.35 AFWVWADD101 pKa = 3.5 GNNAQLTQDD110 pKa = 3.57 AYY112 pKa = 11.64 SLVKK116 pKa = 10.47 SFQSDD121 pKa = 4.25 YY122 pKa = 10.75 IGNYY126 pKa = 9.13 ASKK129 pKa = 10.86 EE130 pKa = 3.98 DD131 pKa = 3.95 FAEE134 pKa = 4.42 EE135 pKa = 3.76 LAKK138 pKa = 10.1 MEE140 pKa = 4.67 NEE142 pKa = 4.18 LSDD145 pKa = 3.58 FALNYY150 pKa = 10.23 FDD152 pKa = 3.98 FSKK155 pKa = 11.2 YY156 pKa = 10.79 ADD158 pKa = 4.7 DD159 pKa = 5.56 LFDD162 pKa = 3.4 MDD164 pKa = 4.7 FWYY167 pKa = 10.83 KK168 pKa = 10.66 DD169 pKa = 3.16 GYY171 pKa = 9.62 VFRR174 pKa = 11.84 NNN176 pKa = 3.2
Molecular weight: 20.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.643
Grimsley 3.516
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.075
Thurlkill 3.643
EMBOSS 3.694
Sillero 3.923
Patrickios 1.863
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|L0JG74|L0JG74_PREDD Bacterial mobilization protein (MobC) OS=Prevotella dentalis (strain ATCC 49559 / DSM 3688 / JCM 13448 / NCTC 12043 / ES 2772) OX=908937 GN=Prede_2624 PE=4 SV=1
MM1 pKa = 7.85 PNGKK5 pKa = 9.19 KK6 pKa = 10.25 KK7 pKa = 10.12 KK8 pKa = 7.0 GHH10 pKa = 6.14 KK11 pKa = 9.06 MATHH15 pKa = 6.13 KK16 pKa = 10.39 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.25 NRR25 pKa = 11.84 HH26 pKa = 4.69 KK27 pKa = 11.1 SKK29 pKa = 11.1
Molecular weight: 3.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.862
IPC_protein 12.325
Toseland 12.53
ProMoST 12.983
Dawson 12.544
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.647
Grimsley 12.574
Solomon 12.998
Lehninger 12.91
Nozaki 12.53
DTASelect 12.501
Thurlkill 12.53
EMBOSS 13.013
Sillero 12.53
Patrickios 12.369
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2494
0
2494
957715
29
3161
384.0
43.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.534 ± 0.05
1.265 ± 0.017
5.852 ± 0.031
5.585 ± 0.047
4.139 ± 0.03
7.485 ± 0.044
2.231 ± 0.022
5.412 ± 0.044
5.131 ± 0.049
9.065 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.808 ± 0.022
4.518 ± 0.039
3.973 ± 0.026
3.827 ± 0.029
5.941 ± 0.049
5.769 ± 0.04
5.985 ± 0.043
6.883 ± 0.035
1.385 ± 0.021
4.212 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here