Vibrio phage henriette 12B8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4MB99|M4MB99_9CAUD HNHc domain-containing protein OS=Vibrio phage henriette 12B8 OX=573174 GN=VPDG_00008 PE=4 SV=1
MM1 pKa = 6.85TTIKK5 pKa = 10.34LYY7 pKa = 10.79NGQTFEE13 pKa = 4.58NMSDD17 pKa = 3.49SDD19 pKa = 4.1RR20 pKa = 11.84QEE22 pKa = 3.86MATVLINRR30 pKa = 11.84EE31 pKa = 3.89FGHH34 pKa = 6.39CFSNVFSTLFAAIDD48 pKa = 3.96EE49 pKa = 4.67SHH51 pKa = 7.01VEE53 pKa = 3.91YY54 pKa = 11.08DD55 pKa = 5.7DD56 pKa = 3.77MQTLQGAYY64 pKa = 10.15DD65 pKa = 3.92YY66 pKa = 7.36TTVVTDD72 pKa = 4.83HH73 pKa = 7.07INDD76 pKa = 3.54LSLYY80 pKa = 10.75DD81 pKa = 5.01LLEE84 pKa = 4.22LVNNEE89 pKa = 4.16SLDD92 pKa = 3.63DD93 pKa = 3.87TRR95 pKa = 11.84EE96 pKa = 3.9MMHH99 pKa = 6.49TSLVKK104 pKa = 10.31DD105 pKa = 3.82LKK107 pKa = 10.77NYY109 pKa = 9.59LVEE112 pKa = 4.1YY113 pKa = 9.75VKK115 pKa = 10.36WDD117 pKa = 3.36WKK119 pKa = 10.57QSTIEE124 pKa = 4.62DD125 pKa = 3.89VFSSYY130 pKa = 11.28SGDD133 pKa = 3.41EE134 pKa = 3.96QFMHH138 pKa = 7.42DD139 pKa = 4.58EE140 pKa = 4.87DD141 pKa = 5.38IEE143 pKa = 5.64DD144 pKa = 2.89IHH146 pKa = 8.56QYY148 pKa = 8.06MDD150 pKa = 2.8RR151 pKa = 11.84VMPEE155 pKa = 3.7VVNYY159 pKa = 9.98FSDD162 pKa = 4.28IDD164 pKa = 3.86NLDD167 pKa = 3.67NFIDD171 pKa = 3.5WAEE174 pKa = 4.27TNNEE178 pKa = 3.75LSDD181 pKa = 4.27WIGTDD186 pKa = 3.29LDD188 pKa = 4.33TIRR191 pKa = 11.84AACFEE196 pKa = 5.06SISSSDD202 pKa = 4.92DD203 pKa = 3.48DD204 pKa = 4.23LQNYY208 pKa = 9.41SNNNGLDD215 pKa = 3.36AEE217 pKa = 4.22FEE219 pKa = 4.03EE220 pKa = 6.26AYY222 pKa = 9.76EE223 pKa = 4.01HH224 pKa = 6.72WIVSSWLSDD233 pKa = 3.36KK234 pKa = 11.09LPNTGDD240 pKa = 3.49VCGLTIWARR249 pKa = 11.84YY250 pKa = 7.14CTGQSICLDD259 pKa = 3.6YY260 pKa = 11.2NIQQLAFDD268 pKa = 5.24LYY270 pKa = 10.98SSEE273 pKa = 4.03VDD275 pKa = 3.01EE276 pKa = 5.23GEE278 pKa = 4.49HH279 pKa = 5.63IKK281 pKa = 10.84KK282 pKa = 10.15VDD284 pKa = 4.08AIKK287 pKa = 10.73AVIDD291 pKa = 4.0ASVKK295 pKa = 9.49PDD297 pKa = 3.13MVEE300 pKa = 3.95YY301 pKa = 9.39VSKK304 pKa = 11.14VIFDD308 pKa = 3.41NTTAYY313 pKa = 10.0QCAIDD318 pKa = 5.76ASDD321 pKa = 4.36LDD323 pKa = 3.84YY324 pKa = 11.31DD325 pKa = 4.01QRR327 pKa = 11.84KK328 pKa = 9.17ALEE331 pKa = 4.13KK332 pKa = 10.41RR333 pKa = 11.84ISSNVKK339 pKa = 9.53KK340 pKa = 10.94VNTVLEE346 pKa = 4.23SHH348 pKa = 6.35NLSLL352 pKa = 5.26

Molecular weight: 40.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4MCW2|M4MCW2_9CAUD Uncharacterized protein OS=Vibrio phage henriette 12B8 OX=573174 GN=VPDG_00040 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.86GNMTQEE8 pKa = 3.59YY9 pKa = 9.23KK10 pKa = 10.35IYY12 pKa = 10.54VEE14 pKa = 4.05HH15 pKa = 6.73FRR17 pKa = 11.84SKK19 pKa = 10.18RR20 pKa = 11.84CRR22 pKa = 11.84KK23 pKa = 9.31ILRR26 pKa = 11.84KK27 pKa = 8.13TYY29 pKa = 10.3RR30 pKa = 11.84FSPEE34 pKa = 3.48LAIKK38 pKa = 9.59TMHH41 pKa = 6.04QAKK44 pKa = 9.62NQLARR49 pKa = 11.84WDD51 pKa = 3.52RR52 pKa = 11.84AANKK56 pKa = 9.45LRR58 pKa = 11.84QCTRR62 pKa = 11.84DD63 pKa = 3.44YY64 pKa = 11.65DD65 pKa = 3.89VLDD68 pKa = 3.45WGVII72 pKa = 3.6

Molecular weight: 8.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

154

0

154

31838

42

1227

206.7

23.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.341 ± 0.263

1.684 ± 0.119

7.095 ± 0.147

6.772 ± 0.227

3.958 ± 0.115

6.637 ± 0.23

2.261 ± 0.103

6.552 ± 0.143

6.828 ± 0.251

7.513 ± 0.186

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.169 ± 0.129

5.613 ± 0.153

3.351 ± 0.146

3.172 ± 0.162

4.429 ± 0.135

6.238 ± 0.216

6.159 ± 0.251

6.643 ± 0.142

1.426 ± 0.089

4.159 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski