Aquimixticola soesokkakensis
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3316 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y5T5N1|A0A1Y5T5N1_9RHOB Asp/Glu/Hydantoin racemase OS=Aquimixticola soesokkakensis OX=1519096 GN=AQS8620_02469 PE=4 SV=1
MM1 pKa = 7.69 KK2 pKa = 10.06 ILVSSSVLALCATAAFADD20 pKa = 4.06 CTEE23 pKa = 4.13 VSFSDD28 pKa = 4.63 VGWTDD33 pKa = 2.5 ITTTTTIAKK42 pKa = 10.02 QILEE46 pKa = 4.03 PLGYY50 pKa = 10.17 KK51 pKa = 10.05 VDD53 pKa = 3.8 VSVLSVPVTFASLSGGDD70 pKa = 3.29 TDD72 pKa = 4.57 IFLGNWMPAQSGAVTPYY89 pKa = 10.93 LEE91 pKa = 5.4 DD92 pKa = 3.43 GTIEE96 pKa = 4.1 SLHH99 pKa = 6.71 EE100 pKa = 4.05 NLQGTVYY107 pKa = 9.88 TLAVPAYY114 pKa = 8.51 TYY116 pKa = 11.04 EE117 pKa = 4.55 KK118 pKa = 10.34 GLKK121 pKa = 10.1 DD122 pKa = 3.59 FADD125 pKa = 3.32 IATFSDD131 pKa = 3.59 ALDD134 pKa = 3.56 DD135 pKa = 4.24 TIYY138 pKa = 10.84 GIEE141 pKa = 4.61 PGNEE145 pKa = 3.73 GNDD148 pKa = 3.48 YY149 pKa = 10.87 LISLTEE155 pKa = 4.34 ADD157 pKa = 3.52 THH159 pKa = 6.16 GLGGFDD165 pKa = 3.28 VKK167 pKa = 10.76 EE168 pKa = 4.27 SSEE171 pKa = 4.01 QGMLSQVRR179 pKa = 11.84 RR180 pKa = 11.84 AYY182 pKa = 10.46 DD183 pKa = 3.33 KK184 pKa = 11.77 NEE186 pKa = 4.86 DD187 pKa = 3.47 IVFLGWAPHH196 pKa = 5.62 PMNVEE201 pKa = 3.86 FDD203 pKa = 3.91 LEE205 pKa = 4.32 YY206 pKa = 10.43 LTGGEE211 pKa = 4.42 DD212 pKa = 3.52 FFGGVGVVNTLTRR225 pKa = 11.84 TGFAQEE231 pKa = 4.4 CPNVGKK237 pKa = 10.59 LLTNLDD243 pKa = 3.73 FTVEE247 pKa = 4.08 MEE249 pKa = 4.22 SQIMGAILTDD259 pKa = 4.29 GEE261 pKa = 4.54 DD262 pKa = 3.44 ADD264 pKa = 4.98 DD265 pKa = 4.66 AVSAYY270 pKa = 8.13 ITANPGVLDD279 pKa = 3.44 AWLEE283 pKa = 4.14 GVTTLEE289 pKa = 4.4 GDD291 pKa = 3.38 AGLPAVKK298 pKa = 9.86 AAYY301 pKa = 9.97 GLL303 pKa = 3.86
Molecular weight: 32.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.541
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.452
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.872
Patrickios 1.189
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A1Y5SZ26|A0A1Y5SZ26_9RHOB Methylated-DNA--[protein]-cysteine S-methyltransferase OS=Aquimixticola soesokkakensis OX=1519096 GN=ada PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.34 SLSAA44 pKa = 3.83
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3316
0
3316
1038695
30
1526
313.2
33.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.89 ± 0.064
0.897 ± 0.011
6.07 ± 0.039
5.458 ± 0.043
3.786 ± 0.025
8.495 ± 0.04
1.976 ± 0.021
5.068 ± 0.029
3.282 ± 0.035
10.015 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.745 ± 0.022
2.549 ± 0.02
4.956 ± 0.027
3.422 ± 0.026
6.41 ± 0.047
5.413 ± 0.035
5.697 ± 0.03
7.382 ± 0.035
1.295 ± 0.016
2.194 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here