Chlamydiales bacterium SCGC AG-110-M15
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 847 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A212KU77|A0A212KU77_9CHLA Translation initiation factor IF-3 OS=Chlamydiales bacterium SCGC AG-110-M15 OX=1871324 GN=infC PE=3 SV=1
MM1 pKa = 8.1 DD2 pKa = 5.73 CMDD5 pKa = 5.47 CMDD8 pKa = 5.57 CMDD11 pKa = 5.57 CMDD14 pKa = 5.57 CMDD17 pKa = 5.57 CMDD20 pKa = 5.57 CMDD23 pKa = 5.57 CMDD26 pKa = 5.57 CMDD29 pKa = 5.57 CMDD32 pKa = 5.57 CMDD35 pKa = 5.57 CMDD38 pKa = 5.52 CMDD41 pKa = 5.38 CMEE44 pKa = 4.96 TDD46 pKa = 3.65 LKK48 pKa = 11.39 FEE50 pKa = 4.02 ISEE53 pKa = 4.14 QEE55 pKa = 3.85 KK56 pKa = 8.96 TPEE59 pKa = 3.64 KK60 pKa = 10.78 SRR62 pKa = 11.84 VLRR65 pKa = 11.84 KK66 pKa = 9.76 ISVFCC71 pKa = 4.8
Molecular weight: 8.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.877
IPC2_protein 3.63
IPC_protein 3.63
Toseland 3.401
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.63
Rodwell 3.452
Grimsley 3.312
Solomon 3.617
Lehninger 3.579
Nozaki 3.77
DTASelect 4.037
Thurlkill 3.478
EMBOSS 3.63
Sillero 3.757
Patrickios 0.006
IPC_peptide 3.617
IPC2_peptide 3.719
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A212KTN1|A0A212KTN1_9CHLA Uncharacterized protein OS=Chlamydiales bacterium SCGC AG-110-M15 OX=1871324 GN=SCG7109_AK_00130 PE=4 SV=1
MM1 pKa = 7.56 WNICGEE7 pKa = 4.03 QSVPSSLASDD17 pKa = 3.84 RR18 pKa = 11.84 VYY20 pKa = 10.39 NTSNLFEE27 pKa = 4.81 YY28 pKa = 9.84 GHH30 pKa = 6.52 LMKK33 pKa = 10.72 AAFHH37 pKa = 6.61 PVHH40 pKa = 5.59 QVKK43 pKa = 10.33 PGGGLFSVALSVALRR58 pKa = 11.84 PPVFHH63 pKa = 6.57 WHH65 pKa = 6.26 PLLRR69 pKa = 11.84 RR70 pKa = 11.84 PDD72 pKa = 4.15 FPPLLPINRR81 pKa = 11.84 QKK83 pKa = 10.88 RR84 pKa = 11.84 RR85 pKa = 3.34
Molecular weight: 9.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.736
IPC_protein 10.599
Toseland 10.672
ProMoST 10.906
Dawson 10.774
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.965
Grimsley 10.833
Solomon 10.906
Lehninger 10.877
Nozaki 10.657
DTASelect 10.496
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.701
Patrickios 10.774
IPC_peptide 10.906
IPC2_peptide 9.589
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
847
0
847
277359
29
5397
327.5
36.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.144 ± 0.078
1.149 ± 0.042
5.836 ± 0.14
7.345 ± 0.137
4.497 ± 0.062
6.465 ± 0.099
2.444 ± 0.049
6.569 ± 0.081
6.653 ± 0.145
10.454 ± 0.099
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.344 ± 0.056
4.223 ± 0.073
3.867 ± 0.056
3.753 ± 0.068
5.011 ± 0.085
7.214 ± 0.083
4.908 ± 0.126
5.922 ± 0.086
1.004 ± 0.028
3.173 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here