Porcine associated porprismacovirus 8

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076VH53|A0A076VH53_9VIRU Cap protein OS=Porcine associated porprismacovirus 8 OX=2170124 PE=4 SV=1
MM1 pKa = 8.17VMVRR5 pKa = 11.84VSEE8 pKa = 4.34TYY10 pKa = 10.7DD11 pKa = 4.06LSTKK15 pKa = 6.66TNKK18 pKa = 9.32MGIVGIHH25 pKa = 5.16TPRR28 pKa = 11.84GNLIDD33 pKa = 3.94QMWPGLVLQHH43 pKa = 6.23KK44 pKa = 9.87KK45 pKa = 10.34FRR47 pKa = 11.84FVQCDD52 pKa = 3.04VAMACASMLPADD64 pKa = 4.26PLQIGVEE71 pKa = 4.06AGAIAPQDD79 pKa = 3.54MFNPILYY86 pKa = 9.77KK87 pKa = 10.6AVSNDD92 pKa = 2.66SMSQVLQYY100 pKa = 10.65LAQAQQTGSGTTNTGLLQGSVASVNDD126 pKa = 3.34VAFKK130 pKa = 10.99ADD132 pKa = 4.13AEE134 pKa = 4.38DD135 pKa = 3.97TVNIDD140 pKa = 3.57QFNIYY145 pKa = 10.03YY146 pKa = 10.77GLLADD151 pKa = 3.65SDD153 pKa = 4.19GWRR156 pKa = 11.84KK157 pKa = 10.0AMPQAGLEE165 pKa = 4.04MRR167 pKa = 11.84GLYY170 pKa = 9.89PLVFSVMSQFGTNGYY185 pKa = 5.65TTGEE189 pKa = 4.34GVSGGTMDD197 pKa = 5.4AEE199 pKa = 4.59SLNHH203 pKa = 5.87QYY205 pKa = 11.33GMSASSDD212 pKa = 2.82LSTYY216 pKa = 10.52RR217 pKa = 11.84NSLMRR222 pKa = 11.84GPTMRR227 pKa = 11.84MPALDD232 pKa = 3.78TVCYY236 pKa = 10.2NPNDD240 pKa = 3.66VSSGDD245 pKa = 3.6VVPNSNALVHH255 pKa = 6.56SNVGEE260 pKa = 4.07VPPAYY265 pKa = 10.15VGLIVLPPAKK275 pKa = 10.23LNQLYY280 pKa = 10.26YY281 pKa = 10.12RR282 pKa = 11.84LKK284 pKa = 9.08VTWTVEE290 pKa = 4.01FSGLRR295 pKa = 11.84SMNDD299 pKa = 2.73IAKK302 pKa = 8.87WAMLGRR308 pKa = 11.84IGSSSYY314 pKa = 9.5GTDD317 pKa = 3.04YY318 pKa = 11.69ANQSRR323 pKa = 11.84SMPEE327 pKa = 3.25QTAMVDD333 pKa = 3.14TGGAAIEE340 pKa = 4.8KK341 pKa = 10.05IMEE344 pKa = 4.37GAA346 pKa = 3.51

Molecular weight: 37.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076VH53|A0A076VH53_9VIRU Cap protein OS=Porcine associated porprismacovirus 8 OX=2170124 PE=4 SV=1
MM1 pKa = 6.91KK2 pKa = 9.8TYY4 pKa = 9.95MLTIPRR10 pKa = 11.84SVPKK14 pKa = 10.05RR15 pKa = 11.84AIMTMIEE22 pKa = 4.06VNDD25 pKa = 3.84CKK27 pKa = 10.59RR28 pKa = 11.84WIVAKK33 pKa = 8.98EE34 pKa = 4.14TGHH37 pKa = 6.63GGYY40 pKa = 10.08DD41 pKa = 2.71HH42 pKa = 6.47WQIRR46 pKa = 11.84LQTSNDD52 pKa = 3.92SFFEE56 pKa = 3.86WCEE59 pKa = 3.64QHH61 pKa = 6.95IPTAHH66 pKa = 6.48VEE68 pKa = 3.93EE69 pKa = 4.98AQNKK73 pKa = 7.47WEE75 pKa = 4.04YY76 pKa = 9.59EE77 pKa = 4.06RR78 pKa = 11.84KK79 pKa = 9.81EE80 pKa = 4.02GRR82 pKa = 11.84FWTSDD87 pKa = 3.19DD88 pKa = 3.43TTEE91 pKa = 3.99IRR93 pKa = 11.84IEE95 pKa = 4.0RR96 pKa = 11.84FGRR99 pKa = 11.84PRR101 pKa = 11.84GWQEE105 pKa = 3.45TALKK109 pKa = 10.41RR110 pKa = 11.84LRR112 pKa = 11.84TQNDD116 pKa = 3.26RR117 pKa = 11.84QVDD120 pKa = 3.36VWYY123 pKa = 10.2DD124 pKa = 3.53PIGNRR129 pKa = 11.84GKK131 pKa = 10.28SWLAGHH137 pKa = 7.38LFEE140 pKa = 5.26TGKK143 pKa = 10.86ACIVPRR149 pKa = 11.84DD150 pKa = 3.54WTNPSEE156 pKa = 3.9ICNYY160 pKa = 9.93LVHH163 pKa = 6.97NYY165 pKa = 9.94DD166 pKa = 3.58NEE168 pKa = 4.63PIIVIDD174 pKa = 3.74IPRR177 pKa = 11.84EE178 pKa = 3.68TGIPKK183 pKa = 10.24GFYY186 pKa = 9.63ATVEE190 pKa = 4.2MIKK193 pKa = 10.63DD194 pKa = 3.64GLIGTTKK201 pKa = 9.84WEE203 pKa = 3.77GRR205 pKa = 11.84MRR207 pKa = 11.84NIRR210 pKa = 11.84GVKK213 pKa = 9.23IIVMTNHH220 pKa = 6.04QMDD223 pKa = 4.66LKK225 pKa = 10.83KK226 pKa = 10.59LSKK229 pKa = 10.68DD230 pKa = 2.65RR231 pKa = 11.84WRR233 pKa = 11.84LNGINAQNAPMLL245 pKa = 4.27

Molecular weight: 28.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

591

245

346

295.5

33.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.768 ± 1.66

1.184 ± 0.277

5.753 ± 0.024

4.907 ± 1.508

2.369 ± 0.05

8.291 ± 0.836

1.861 ± 0.615

5.922 ± 1.889

4.569 ± 1.213

6.768 ± 1.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

5.076 ± 0.867

5.584 ± 0.081

4.738 ± 0.153

4.569 ± 0.553

5.584 ± 1.846

6.261 ± 2.103

6.599 ± 0.462

6.937 ± 1.26

2.538 ± 1.206

3.723 ± 0.535
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski