Capybara microvirus Cap3_SP_632
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVV2|A0A4V1FVV2_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_632 OX=2585484 PE=4 SV=1
MM1 pKa = 7.74 KK2 pKa = 10.1 FQSKK6 pKa = 9.14 FNRR9 pKa = 11.84 FAKK12 pKa = 10.18 VFSKK16 pKa = 10.73 AGEE19 pKa = 3.96 PTVTVNILNIDD30 pKa = 3.56 GSLAEE35 pKa = 4.82 SYY37 pKa = 10.75 IDD39 pKa = 5.73 DD40 pKa = 4.95 LDD42 pKa = 5.2 ALIQSNADD50 pKa = 3.37 SGDD53 pKa = 3.29 VSKK56 pKa = 11.12 LIEE59 pKa = 4.51 RR60 pKa = 11.84 FTLTGDD66 pKa = 3.4 DD67 pKa = 3.7 SVFNVSNGFYY77 pKa = 10.68 GDD79 pKa = 3.52 VSGLEE84 pKa = 3.87 NLTIHH89 pKa = 7.57 DD90 pKa = 3.85 YY91 pKa = 9.68 SQRR94 pKa = 11.84 TSAAIDD100 pKa = 4.26 FYY102 pKa = 11.81 DD103 pKa = 4.89 SLPKK107 pKa = 10.23 NIKK110 pKa = 9.54 EE111 pKa = 4.3 MYY113 pKa = 8.85 PSPEE117 pKa = 4.07 EE118 pKa = 3.84 FFKK121 pKa = 11.35 SIDD124 pKa = 3.68 DD125 pKa = 4.18 PKK127 pKa = 11.03 LSEE130 pKa = 4.52 AFDD133 pKa = 3.83 NFLSISNTKK142 pKa = 9.85 TSDD145 pKa = 3.29 SDD147 pKa = 5.42 DD148 pKa = 3.59 EE149 pKa = 6.16 GGDD152 pKa = 3.69 NNASS156 pKa = 3.25
Molecular weight: 17.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.32
IPC2_protein 4.05
IPC_protein 4.037
Toseland 3.821
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.973
Rodwell 3.872
Grimsley 3.732
Solomon 4.024
Lehninger 3.986
Nozaki 4.151
DTASelect 4.406
Thurlkill 3.872
EMBOSS 3.986
Sillero 4.164
Patrickios 3.859
IPC_peptide 4.024
IPC2_peptide 4.139
IPC2.peptide.svr19 4.09
Protein with the highest isoelectric point:
>tr|A0A4P8W694|A0A4P8W694_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_632 OX=2585484 PE=4 SV=1
MM1 pKa = 7.79 ADD3 pKa = 3.27 SYY5 pKa = 12.08 YY6 pKa = 11.32 NDD8 pKa = 3.55 ALKK11 pKa = 10.73 LLKK14 pKa = 10.1 QEE16 pKa = 4.23 QKK18 pKa = 10.6 FSRR21 pKa = 11.84 EE22 pKa = 3.89 TQQLQTNLSNTAHH35 pKa = 5.33 QRR37 pKa = 11.84 EE38 pKa = 4.54 VKK40 pKa = 10.63 DD41 pKa = 3.74 LVAAGLNPVLSSGGSGASFSISEE64 pKa = 4.38 GNSGVASATQYY75 pKa = 10.25 MSAKK79 pKa = 9.71 IAQEE83 pKa = 3.92 TQLAQIAMQKK93 pKa = 10.32 EE94 pKa = 3.97 IAQIQANAQIKK105 pKa = 9.13 AANTSAAATKK115 pKa = 10.17 YY116 pKa = 10.55 AADD119 pKa = 4.01 KK120 pKa = 10.86 NYY122 pKa = 10.46 EE123 pKa = 4.26 SNQYY127 pKa = 8.34 TVDD130 pKa = 3.39 YY131 pKa = 10.42 SRR133 pKa = 11.84 NGSVAGTVGSAAKK146 pKa = 10.01 QIWNSIKK153 pKa = 10.78 KK154 pKa = 9.35 FMPKK158 pKa = 10.23 DD159 pKa = 3.51 NSKK162 pKa = 8.51 TFHH165 pKa = 5.86 WW166 pKa = 4.56
Molecular weight: 17.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.672
IPC2_protein 8.668
IPC_protein 8.609
Toseland 9.297
ProMoST 9.033
Dawson 9.56
Bjellqvist 9.224
Wikipedia 9.736
Rodwell 9.853
Grimsley 9.648
Solomon 9.633
Lehninger 9.604
Nozaki 9.238
DTASelect 9.224
Thurlkill 9.385
EMBOSS 9.706
Sillero 9.472
Patrickios 4.762
IPC_peptide 9.633
IPC2_peptide 7.688
IPC2.peptide.svr19 7.722
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1285
87
578
257.0
29.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.914 ± 1.631
1.634 ± 0.841
7.16 ± 1.03
4.514 ± 0.324
6.693 ± 0.806
6.537 ± 0.307
1.946 ± 0.527
5.681 ± 0.622
5.914 ± 1.196
7.004 ± 0.318
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.556 ± 0.2
6.693 ± 0.316
4.202 ± 0.868
3.424 ± 1.083
4.047 ± 0.726
9.416 ± 0.965
4.903 ± 0.533
5.681 ± 0.382
1.012 ± 0.248
6.07 ± 0.79
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here