Mycobacterium virus Deadp
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8I4W5|G8I4W5_9CAUD Uncharacterized protein OS=Mycobacterium virus Deadp OX=1089120 GN=42 PE=4 SV=1
MM1 pKa = 7.74 AEE3 pKa = 4.27 TVHH6 pKa = 6.3 VLPNNDD12 pKa = 4.67 LIEE15 pKa = 5.93 HH16 pKa = 7.22 DD17 pKa = 5.86 DD18 pKa = 3.94 EE19 pKa = 5.81 GTDD22 pKa = 4.08 CPCGPTVEE30 pKa = 4.71 PVFDD34 pKa = 4.77 ADD36 pKa = 4.73 GACGWVITHH45 pKa = 6.86 HH46 pKa = 6.49 SLDD49 pKa = 3.22 GRR51 pKa = 11.84 EE52 pKa = 3.86 RR53 pKa = 11.84 FEE55 pKa = 3.97
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.866
IPC2_protein 4.151
IPC_protein 4.037
Toseland 3.859
ProMoST 4.139
Dawson 4.024
Bjellqvist 4.253
Wikipedia 3.948
Rodwell 3.884
Grimsley 3.783
Solomon 4.012
Lehninger 3.973
Nozaki 4.151
DTASelect 4.342
Thurlkill 3.91
EMBOSS 3.961
Sillero 4.164
Patrickios 1.952
IPC_peptide 4.012
IPC2_peptide 4.151
IPC2.peptide.svr19 4.083
Protein with the highest isoelectric point:
>tr|G8I4X9|G8I4X9_9CAUD Uncharacterized protein OS=Mycobacterium virus Deadp OX=1089120 GN=56 PE=4 SV=1
MM1 pKa = 7.49 SGRR4 pKa = 11.84 FRR6 pKa = 11.84 TLEE9 pKa = 3.77 QVISEE14 pKa = 4.28 WGFDD18 pKa = 3.34 TSDD21 pKa = 2.99 EE22 pKa = 4.04 EE23 pKa = 3.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 VRR29 pKa = 11.84 WLADD33 pKa = 4.29 LIRR36 pKa = 11.84 AGKK39 pKa = 9.92 VPAHH43 pKa = 6.24 KK44 pKa = 10.21 FGRR47 pKa = 11.84 SWYY50 pKa = 9.72 MDD52 pKa = 3.49 DD53 pKa = 3.69 TDD55 pKa = 3.45 IAAALGVLEE64 pKa = 5.5 NGFQKK69 pKa = 10.53 PVVEE73 pKa = 4.46 RR74 pKa = 11.84 PVEE77 pKa = 3.9 PVRR80 pKa = 11.84 RR81 pKa = 11.84 GPSAASMRR89 pKa = 11.84 RR90 pKa = 11.84 RR91 pKa = 11.84 LASS94 pKa = 3.18
Molecular weight: 10.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.443
IPC_protein 10.57
Toseland 10.833
ProMoST 10.847
Dawson 10.877
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 10.833
Grimsley 10.906
Solomon 11.155
Lehninger 11.096
Nozaki 10.804
DTASelect 10.687
Thurlkill 10.818
EMBOSS 11.272
Sillero 10.833
Patrickios 10.628
IPC_peptide 11.155
IPC2_peptide 9.78
IPC2.peptide.svr19 8.967
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
106
0
106
17860
28
1175
168.5
18.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.047 ± 0.497
1.114 ± 0.15
6.831 ± 0.221
5.857 ± 0.33
3.074 ± 0.209
8.863 ± 0.641
2.122 ± 0.165
4.479 ± 0.219
3.253 ± 0.227
7.29 ± 0.224
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.385 ± 0.14
3.158 ± 0.177
5.868 ± 0.213
3.41 ± 0.186
6.618 ± 0.366
6.148 ± 0.29
6.674 ± 0.24
7.144 ± 0.291
2.251 ± 0.16
2.413 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here