Ilumatobacter coccineus (strain NBRC 103263 / KCTC 29153 / YM16-304)
Average proteome isoelectric point is 5.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4290 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6C7E3C0|A0A6C7E3C0_ILUCY Uncharacterized protein OS=Ilumatobacter coccineus (strain NBRC 103263 / KCTC 29153 / YM16-304) OX=1313172 GN=YM304_05280 PE=4 SV=1
MM1 pKa = 7.47 AAAGLAIPTAINTPIASADD20 pKa = 3.42 AVGSDD25 pKa = 3.93 FEE27 pKa = 4.85 SFALGSVDD35 pKa = 5.24 GQDD38 pKa = 3.04 GWEE41 pKa = 3.79 ISGPYY46 pKa = 9.55 DD47 pKa = 3.68 VEE49 pKa = 4.43 VVDD52 pKa = 6.37 ASATHH57 pKa = 6.66 PSLGNRR63 pKa = 11.84 SLRR66 pKa = 11.84 ISNAVTSGGFGDD78 pKa = 3.81 QTFSKK83 pKa = 10.25 PLTEE87 pKa = 4.4 EE88 pKa = 4.01 AGEE91 pKa = 4.07 TDD93 pKa = 3.69 AEE95 pKa = 4.32 NGGKK99 pKa = 8.03 STGPRR104 pKa = 11.84 QTSFEE109 pKa = 4.42 AEE111 pKa = 3.66 WSFASAAPGAEE122 pKa = 3.97 QPGLSVVVSPDD133 pKa = 3.04 RR134 pKa = 11.84 GDD136 pKa = 3.54 GGRR139 pKa = 11.84 MGWLGMTDD147 pKa = 3.33 TPTGLEE153 pKa = 3.89 VSYY156 pKa = 11.0 YY157 pKa = 10.81 GYY159 pKa = 8.71 DD160 pKa = 3.25 TTLGGTCADD169 pKa = 3.84 LDD171 pKa = 3.77 NFVFTTVATGLDD183 pKa = 3.18 RR184 pKa = 11.84 TAVHH188 pKa = 6.03 TARR191 pKa = 11.84 LRR193 pKa = 11.84 MGFNDD198 pKa = 4.13 GVDD201 pKa = 3.39 NDD203 pKa = 3.94 VVEE206 pKa = 4.44 VFVDD210 pKa = 4.02 GTLVHH215 pKa = 6.63 TGKK218 pKa = 9.9 SWEE221 pKa = 4.2 DD222 pKa = 3.36 YY223 pKa = 10.48 FRR225 pKa = 11.84 NCEE228 pKa = 3.94 PPEE231 pKa = 4.1 PRR233 pKa = 11.84 TVDD236 pKa = 3.12 SLLFRR241 pKa = 11.84 VSGTAAPATSGFGFLIDD258 pKa = 4.29 DD259 pKa = 3.95 VSLEE263 pKa = 4.24 SSSPPPCTTDD273 pKa = 3.42 CYY275 pKa = 11.39 VDD277 pKa = 3.4 AATGSDD283 pKa = 3.71 VNGGTGPADD292 pKa = 3.73 ALRR295 pKa = 11.84 TIQTAIDD302 pKa = 3.38 RR303 pKa = 11.84 VDD305 pKa = 3.34 PNGNVIVAAGTYY317 pKa = 7.43 PVPDD321 pKa = 4.34 GLDD324 pKa = 2.99 VDD326 pKa = 5.03 KK327 pKa = 10.99 PLTLDD332 pKa = 3.52 GAGSDD337 pKa = 3.82 VVTIDD342 pKa = 3.66 ASAHH346 pKa = 5.39 ASYY349 pKa = 10.95 SVAVTADD356 pKa = 3.54 DD357 pKa = 3.66 VTLEE361 pKa = 3.99 GFTLTGNPAGTYY373 pKa = 8.69 GLKK376 pKa = 10.04 ISSSSPTRR384 pKa = 11.84 LSNITVDD391 pKa = 3.95 DD392 pKa = 4.05 VVVNNSKK399 pKa = 9.11 RR400 pKa = 11.84 TGIDD404 pKa = 3.22 LNGVDD409 pKa = 5.21 GGLITNVEE417 pKa = 4.17 VHH419 pKa = 6.79 DD420 pKa = 4.38 VPSGNGLSATDD431 pKa = 3.48 SNDD434 pKa = 2.79 IVFEE438 pKa = 4.07 NVTTSNNAWGGLAIYY453 pKa = 9.05 TYY455 pKa = 10.87 GRR457 pKa = 11.84 YY458 pKa = 9.62 YY459 pKa = 10.86 SIGSNNVTIQGTNSFGEE476 pKa = 4.08 ANPVYY481 pKa = 10.86 VEE483 pKa = 3.99 TGNYY487 pKa = 9.18 NDD489 pKa = 5.1 PGNPAPVTNLTVNGFDD505 pKa = 3.33 YY506 pKa = 10.82 TVANDD511 pKa = 3.26 DD512 pKa = 3.52 HH513 pKa = 6.93 RR514 pKa = 11.84 AGGEE518 pKa = 4.0 LFTFYY523 pKa = 11.16 QNTRR527 pKa = 11.84 SEE529 pKa = 4.61 AIEE532 pKa = 3.9 FALALTNPEE541 pKa = 3.54 SSYY544 pKa = 10.66 VNEE547 pKa = 4.25 VATGDD552 pKa = 3.64 FWVGTIGFDD561 pKa = 5.01 DD562 pKa = 3.97 MTIQAAVDD570 pKa = 3.38 HH571 pKa = 7.04 AEE573 pKa = 4.2 PGDD576 pKa = 3.98 TIRR579 pKa = 11.84 VLPGTYY585 pKa = 9.44 QGNVVVDD592 pKa = 3.75 EE593 pKa = 4.22 HH594 pKa = 7.86 VKK596 pKa = 10.63 IIGSGSGSSEE606 pKa = 3.69 ASNTVLQKK614 pKa = 10.5 SANEE618 pKa = 3.84 ALVTIQASGQSAADD632 pKa = 3.47 PLLFQDD638 pKa = 5.43 LRR640 pKa = 11.84 LEE642 pKa = 4.16 PVGEE646 pKa = 4.0 YY647 pKa = 10.71 GFNVNGPAPVQFVEE661 pKa = 4.21 FDD663 pKa = 3.36 NVKK666 pKa = 10.8 VIGTNSGAAYY676 pKa = 9.18 EE677 pKa = 4.46 AEE679 pKa = 4.28 VGLKK683 pKa = 10.52 VSTNASLSDD692 pKa = 3.69 VVITDD697 pKa = 3.36 SAFDD701 pKa = 3.8 DD702 pKa = 5.39 LVYY705 pKa = 10.46 GWYY708 pKa = 9.24 FAKK711 pKa = 10.68 HH712 pKa = 6.1 GDD714 pKa = 3.44 WGPGGSNVSNVQVTDD729 pKa = 3.38 TSFSNNEE736 pKa = 3.45 VKK738 pKa = 10.72 GIYY741 pKa = 8.78 VEE743 pKa = 4.38 KK744 pKa = 10.92 LSDD747 pKa = 3.37 TTFDD751 pKa = 3.17 NVTVVNNGLDD761 pKa = 3.47 TTFYY765 pKa = 8.93 NAKK768 pKa = 8.6 WNSGFDD774 pKa = 3.3 INLKK778 pKa = 10.81 GEE780 pKa = 4.02 EE781 pKa = 4.48 TYY783 pKa = 11.42 QNITIVNSTFDD794 pKa = 3.64 RR795 pKa = 11.84 NGLGVEE801 pKa = 4.24 EE802 pKa = 4.65 GTGLTIKK809 pKa = 10.68 ARR811 pKa = 11.84 DD812 pKa = 3.54 DD813 pKa = 3.66 GGTYY817 pKa = 9.91 GAHH820 pKa = 6.48 PAEE823 pKa = 4.31 LTGVTITGNDD833 pKa = 2.66 ITGNEE838 pKa = 3.49 RR839 pKa = 11.84 GIRR842 pKa = 11.84 FGEE845 pKa = 4.03 PGKK848 pKa = 10.74 NNATPTDD855 pKa = 3.98 VIVSGNNITGNVVAGVINEE874 pKa = 4.36 TLSQTNAIEE883 pKa = 4.22 NWWGDD888 pKa = 3.04 ASGPSGEE895 pKa = 4.64 GPGTGDD901 pKa = 3.17 SVSLNVSVCPWLDD914 pKa = 3.54 GPAGTGNLVLDD925 pKa = 4.69 DD926 pKa = 4.35 CPGANEE932 pKa = 4.35 LDD934 pKa = 3.93 VVNTVDD940 pKa = 3.43 WNGVTPDD947 pKa = 3.3 AAQTFEE953 pKa = 4.03 ICIEE957 pKa = 4.42 GPSHH961 pKa = 7.05 VVADD965 pKa = 4.06 CQLAGSSTEE974 pKa = 3.94 TLTWSDD980 pKa = 4.89 LYY982 pKa = 11.23 HH983 pKa = 7.02 GDD985 pKa = 3.59 YY986 pKa = 10.66 TVTQTDD992 pKa = 4.21 PGTEE996 pKa = 3.72 WTVDD1000 pKa = 3.11 ITGSPVTVADD1010 pKa = 4.66 GVPSTPANVTNTHH1023 pKa = 6.81 DD1024 pKa = 3.95 PAEE1027 pKa = 4.79 GEE1029 pKa = 4.54 GPTPDD1034 pKa = 4.22 APPTFPATPDD1044 pKa = 4.35 DD1045 pKa = 3.7 ISVVTEE1051 pKa = 4.56 PGASSATVEE1060 pKa = 4.28 YY1061 pKa = 9.33 PLPEE1065 pKa = 4.62 AADD1068 pKa = 4.63 DD1069 pKa = 4.02 DD1070 pKa = 4.88 TEE1072 pKa = 4.24 TLPTVVCTPASGTEE1086 pKa = 4.17 FPVGDD1091 pKa = 3.62 NTVTCTATDD1100 pKa = 3.55 SADD1103 pKa = 3.44 QTAEE1107 pKa = 3.94 TTFTISVSPYY1117 pKa = 9.18 SDD1119 pKa = 5.3 LIAVDD1124 pKa = 3.69 PARR1127 pKa = 11.84 FLDD1130 pKa = 3.75 TRR1132 pKa = 11.84 GTGSNVTIDD1141 pKa = 4.94 GIAQGEE1147 pKa = 4.71 GPVPAGTSITIPVAEE1162 pKa = 4.93 RR1163 pKa = 11.84 GDD1165 pKa = 3.67 VTADD1169 pKa = 3.02 AVGAMVNIAAINPSGNGYY1187 pKa = 7.82 VTLYY1191 pKa = 9.83 PCTDD1195 pKa = 3.78 EE1196 pKa = 4.95 VPTAASLNFEE1206 pKa = 4.58 AGVSISNATFVEE1218 pKa = 4.33 LSDD1221 pKa = 5.45 DD1222 pKa = 3.89 GDD1224 pKa = 3.73 VCAFASNTTGLALDD1238 pKa = 3.77 VVGYY1242 pKa = 8.95 MPVTSRR1248 pKa = 11.84 IDD1250 pKa = 3.32 FVAPARR1256 pKa = 11.84 LLEE1259 pKa = 3.98 TRR1261 pKa = 11.84 IGEE1264 pKa = 4.34 GLTTIDD1270 pKa = 4.62 GRR1272 pKa = 11.84 SQGDD1276 pKa = 3.7 GAITAGNTIEE1286 pKa = 4.14 VQVAGRR1292 pKa = 11.84 AGVADD1297 pKa = 4.1 DD1298 pKa = 5.14 AVAAMVNVAAVNPTDD1313 pKa = 3.04 TGYY1316 pKa = 8.45 ITLFPCDD1323 pKa = 3.47 SEE1325 pKa = 4.93 QPVAASLNYY1334 pKa = 9.12 TAGTNISNTTLVALSSDD1351 pKa = 3.19 GVLCVFSEE1359 pKa = 4.44 DD1360 pKa = 3.89 ATDD1363 pKa = 3.65 LTIDD1367 pKa = 3.76 VIGYY1371 pKa = 8.85 ASPGTDD1377 pKa = 2.53 VGTLNPSRR1385 pKa = 11.84 LLEE1388 pKa = 4.21 TRR1390 pKa = 11.84 TGTGQVTVDD1399 pKa = 5.23 GIAQGDD1405 pKa = 4.11 GQVDD1409 pKa = 3.4 AGHH1412 pKa = 7.0 FISVPIAGRR1421 pKa = 11.84 SDD1423 pKa = 3.55 VPDD1426 pKa = 3.94 DD1427 pKa = 4.93 AIAVVVNLAAINPAAVGYY1445 pKa = 7.75 ATLYY1449 pKa = 9.96 PCTEE1453 pKa = 4.31 TPPVAASLNYY1463 pKa = 9.64 RR1464 pKa = 11.84 AGVNISNATLVDD1476 pKa = 4.28 LSDD1479 pKa = 5.08 DD1480 pKa = 3.8 GDD1482 pKa = 3.77 VCLFTSATAHH1492 pKa = 4.75 YY1493 pKa = 8.53 TIDD1496 pKa = 3.32 VLSFVTATSVDD1507 pKa = 3.35 AA1508 pKa = 5.22
Molecular weight: 155.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.401
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.859
Patrickios 1.329
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|A0A6C7EAY4|A0A6C7EAY4_ILUCY UPF0434 protein YM304_28480 OS=Ilumatobacter coccineus (strain NBRC 103263 / KCTC 29153 / YM16-304) OX=1313172 GN=YM304_28480 PE=3 SV=1
MM1 pKa = 7.57 GSLIKK6 pKa = 10.25 KK7 pKa = 8.32 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.27 RR11 pKa = 11.84 MRR13 pKa = 11.84 KK14 pKa = 8.96 KK15 pKa = 9.73 KK16 pKa = 8.93 HH17 pKa = 5.59 KK18 pKa = 10.34 KK19 pKa = 6.44 MLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 HH26 pKa = 4.1 QRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.0
Molecular weight: 3.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4290
0
4290
1492228
30
5213
347.8
37.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.205 ± 0.05
0.829 ± 0.012
7.756 ± 0.042
5.784 ± 0.038
3.203 ± 0.022
8.858 ± 0.043
2.136 ± 0.022
4.669 ± 0.024
1.83 ± 0.029
9.033 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.035 ± 0.019
2.322 ± 0.027
5.465 ± 0.03
2.566 ± 0.02
6.7 ± 0.055
5.982 ± 0.035
6.559 ± 0.066
8.821 ± 0.04
1.42 ± 0.015
1.827 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here