St Croix River virus
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9DSP3|Q9DSP3_9REOV NS2 OS=St Croix River virus OX=104581 PE=4 SV=1
MM1 pKa = 7.6 SDD3 pKa = 2.72 SRR5 pKa = 11.84 AYY7 pKa = 10.33 AIEE10 pKa = 4.07 ILDD13 pKa = 4.04 LFADD17 pKa = 3.92 QVPPLPGGDD26 pKa = 3.32 SRR28 pKa = 11.84 ALSRR32 pKa = 11.84 ARR34 pKa = 11.84 AFLRR38 pKa = 11.84 NPIVVRR44 pKa = 11.84 NYY46 pKa = 10.54 PNLDD50 pKa = 3.44 PLGNDD55 pKa = 2.66 ATVRR59 pKa = 11.84 RR60 pKa = 11.84 NLFFVCLDD68 pKa = 3.47 VAMLALDD75 pKa = 3.26 INEE78 pKa = 3.91 FKK80 pKa = 11.15 VRR82 pKa = 11.84 IEE84 pKa = 3.76 SHH86 pKa = 5.25 RR87 pKa = 11.84 VARR90 pKa = 11.84 EE91 pKa = 3.49 LAALLGPTGADD102 pKa = 3.43 PQPTAVQIAEE112 pKa = 4.19 SFTDD116 pKa = 3.29 KK117 pKa = 11.15 GRR119 pKa = 11.84 TTYY122 pKa = 11.18 AVGQDD127 pKa = 3.22 GPVYY131 pKa = 10.49 IEE133 pKa = 4.33 GAYY136 pKa = 10.36 SPSPFTYY143 pKa = 9.97 GPRR146 pKa = 11.84 YY147 pKa = 8.6 VQDD150 pKa = 3.49 GNFVNVVGPVRR161 pKa = 11.84 DD162 pKa = 3.74 GAAPPEE168 pKa = 4.28 PCFLVANSPGRR179 pKa = 11.84 VTVILNGGPNSLALEE194 pKa = 4.61 HH195 pKa = 6.81 IFRR198 pKa = 11.84 TILRR202 pKa = 11.84 RR203 pKa = 11.84 GSLFTARR210 pKa = 11.84 WYY212 pKa = 10.92 ANSCFIGEE220 pKa = 4.15 NGAPIITNGVPSLTSGGGGTNIPAYY245 pKa = 10.58 FPFTFSKK252 pKa = 10.64 RR253 pKa = 11.84 STFTARR259 pKa = 11.84 VRR261 pKa = 11.84 GGGAWPVAAITIEE274 pKa = 3.93 FLSFYY279 pKa = 10.55 SIGHH283 pKa = 6.48 FGDD286 pKa = 4.82 PYY288 pKa = 10.76 PGFSTDD294 pKa = 3.37 LQDD297 pKa = 4.89 FYY299 pKa = 11.11 TYY301 pKa = 10.64 TNPVWHH307 pKa = 7.62 ALRR310 pKa = 11.84 KK311 pKa = 8.86 QLSSAMDD318 pKa = 3.96 LSMYY322 pKa = 10.51 VATKK326 pKa = 9.95 YY327 pKa = 11.23 DD328 pKa = 3.73 DD329 pKa = 3.79 FTRR332 pKa = 11.84 HH333 pKa = 5.59 QMLALALFGRR343 pKa = 11.84 LSRR346 pKa = 11.84 IFRR349 pKa = 11.84 RR350 pKa = 11.84 LDD352 pKa = 3.24 PDD354 pKa = 4.28 LRR356 pKa = 11.84 VLPLDD361 pKa = 4.11 NGDD364 pKa = 4.36 GEE366 pKa = 4.63 AEE368 pKa = 4.1 AYY370 pKa = 9.86 AAEE373 pKa = 4.33 AAVEE377 pKa = 4.17 EE378 pKa = 4.57 AA379 pKa = 4.7
Molecular weight: 41.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.533
IPC2_protein 5.588
IPC_protein 5.588
Toseland 5.855
ProMoST 5.868
Dawson 5.753
Bjellqvist 5.804
Wikipedia 5.728
Rodwell 5.715
Grimsley 5.957
Solomon 5.753
Lehninger 5.741
Nozaki 5.97
DTASelect 6.173
Thurlkill 6.084
EMBOSS 6.059
Sillero 6.071
Patrickios 4.037
IPC_peptide 5.766
IPC2_peptide 6.071
IPC2.peptide.svr19 6.064
Protein with the highest isoelectric point:
>tr|Q9DSP1|Q9DSP1_9REOV VP6 OS=St Croix River virus OX=104581 PE=4 SV=1
MM1 pKa = 7.31 QKK3 pKa = 10.03 QSHH6 pKa = 5.82 LTMMPRR12 pKa = 11.84 PTAVVMTKK20 pKa = 10.64 DD21 pKa = 3.38 GIGAEE26 pKa = 4.36 RR27 pKa = 11.84 VTLMPSAPSVSPLQVISEE45 pKa = 4.41 TLSVPSAKK53 pKa = 9.66 TPPEE57 pKa = 4.0 RR58 pKa = 11.84 MEE60 pKa = 3.89 KK61 pKa = 10.44 AALKK65 pKa = 10.0 GAQEE69 pKa = 4.91 AISDD73 pKa = 3.86 SSDD76 pKa = 3.89 PIGMLIKK83 pKa = 10.63 VEE85 pKa = 4.59 ANASTISALQHH96 pKa = 5.67 GEE98 pKa = 3.82 RR99 pKa = 11.84 KK100 pKa = 9.1 LARR103 pKa = 11.84 RR104 pKa = 11.84 KK105 pKa = 10.15 SLLFWLHH112 pKa = 5.73 LTTVILMLVFTAISSIANLSIHH134 pKa = 6.53 LEE136 pKa = 4.09 HH137 pKa = 6.9 YY138 pKa = 10.94 LRR140 pKa = 11.84 MANLSSNMICLGFATSTMFITRR162 pKa = 11.84 TRR164 pKa = 11.84 LSVALEE170 pKa = 3.64 LHH172 pKa = 6.19 KK173 pKa = 10.83 VRR175 pKa = 11.84 KK176 pKa = 7.87 QLRR179 pKa = 11.84 KK180 pKa = 9.45 RR181 pKa = 11.84 LAYY184 pKa = 9.89 QATAKK189 pKa = 10.52 NISAARR195 pKa = 11.84 APPPSQPFLSDD206 pKa = 3.31 VSSGTSGWIVAPGCKK221 pKa = 9.72 ALQQ224 pKa = 3.55
Molecular weight: 24.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.214
IPC2_protein 9.472
IPC_protein 9.589
Toseland 10.613
ProMoST 10.101
Dawson 10.687
Bjellqvist 10.277
Wikipedia 10.804
Rodwell 11.155
Grimsley 10.701
Solomon 10.73
Lehninger 10.73
Nozaki 10.584
DTASelect 10.277
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.599
Patrickios 10.906
IPC_peptide 10.745
IPC2_peptide 8.887
IPC2.peptide.svr19 8.598
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
5863
224
1345
586.3
65.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.846 ± 0.771
0.955 ± 0.151
5.611 ± 0.381
5.151 ± 0.41
4.844 ± 0.364
5.987 ± 0.251
3.087 ± 0.271
5.373 ± 0.35
3.275 ± 0.318
10.387 ± 0.362
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.354 ± 0.193
2.729 ± 0.247
6.55 ± 0.448
3.377 ± 0.297
7.266 ± 0.315
9.006 ± 0.442
5.526 ± 0.448
6.669 ± 0.24
0.972 ± 0.222
3.036 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here