Plesiomonas phage phiP4-7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6PG24|A0A2I6PG24_9CAUD Uncharacterized protein OS=Plesiomonas phage phiP4-7 OX=2065204 GN=phiP47_044 PE=4 SV=1
MM1 pKa = 7.44TEE3 pKa = 4.95RR4 pKa = 11.84EE5 pKa = 4.46SGWYY9 pKa = 9.07RR10 pKa = 11.84AVVRR14 pKa = 11.84YY15 pKa = 9.18GSWQLFEE22 pKa = 4.01WDD24 pKa = 4.25ANEE27 pKa = 4.96GWVTDD32 pKa = 3.84CGLIKK37 pKa = 10.94YY38 pKa = 10.02HH39 pKa = 7.6DD40 pKa = 4.83DD41 pKa = 3.9DD42 pKa = 5.01FSEE45 pKa = 4.81IDD47 pKa = 3.58PQMVMTPSGEE57 pKa = 4.24MVYY60 pKa = 10.2HH61 pKa = 6.92RR62 pKa = 11.84SEE64 pKa = 4.41HH65 pKa = 6.54DD66 pKa = 3.44PLYY69 pKa = 10.2NTSQEE74 pKa = 4.13GG75 pKa = 3.26

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6PG23|A0A2I6PG23_9CAUD Uncharacterized protein OS=Plesiomonas phage phiP4-7 OX=2065204 GN=phiP47_045 PE=4 SV=1
MM1 pKa = 7.78APPIKK6 pKa = 9.64RR7 pKa = 11.84TQHH10 pKa = 6.55DD11 pKa = 4.09VIEE14 pKa = 4.34YY15 pKa = 10.66LLSQWPTRR23 pKa = 11.84PAGRR27 pKa = 11.84LASMASRR34 pKa = 11.84YY35 pKa = 9.73AAFKK39 pKa = 10.87GMNCKK44 pKa = 10.26EE45 pKa = 3.84ITPFFQIRR53 pKa = 11.84MLTGEE58 pKa = 4.02NRR60 pKa = 11.84GSKK63 pKa = 10.12VMPNQQ68 pKa = 3.17

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

12420

37

963

172.5

19.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.573 ± 0.463

1.506 ± 0.199

6.167 ± 0.218

5.974 ± 0.285

3.671 ± 0.215

7.085 ± 0.271

1.763 ± 0.144

6.433 ± 0.318

6.634 ± 0.309

7.536 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.253 ± 0.163

4.356 ± 0.225

3.599 ± 0.189

4.541 ± 0.263

5.306 ± 0.217

5.829 ± 0.254

5.805 ± 0.266

6.602 ± 0.233

1.304 ± 0.112

3.06 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski