Rhodoplanes elegans
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5973 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A327KY74|A0A327KY74_9RHIZ Xanthine dehydrogenase OS=Rhodoplanes elegans OX=29408 GN=CH338_00985 PE=4 SV=1
MM1 pKa = 7.29 SLSGALNSAVSALNSQSQAISMVSDD26 pKa = 4.35 NIANASTTGYY36 pKa = 8.56 KK37 pKa = 9.3 TVTASFEE44 pKa = 4.45 SLLSSSSSSAGYY56 pKa = 10.54 ASGGVAVSARR66 pKa = 11.84 YY67 pKa = 9.43 NISEE71 pKa = 4.1 QGLLTSSTSDD81 pKa = 2.54 TAMAIDD87 pKa = 3.91 GNGFFVVRR95 pKa = 11.84 DD96 pKa = 3.84 ASGSTYY102 pKa = 7.56 YY103 pKa = 9.98 TRR105 pKa = 11.84 NGDD108 pKa = 4.53 AVIDD112 pKa = 4.13 DD113 pKa = 4.18 ANGYY117 pKa = 7.89 LTIGGNYY124 pKa = 9.49 LMGWPTDD131 pKa = 3.16 QDD133 pKa = 3.91 GNVTGGEE140 pKa = 4.04 TAATLTRR147 pKa = 11.84 IDD149 pKa = 3.5 TSALQSVASATTTEE163 pKa = 4.33 IIEE166 pKa = 4.47 ANLPADD172 pKa = 3.8 AATNATFTTTMDD184 pKa = 3.44 VYY186 pKa = 11.34 DD187 pKa = 4.05 SLGTASSVTITWEE200 pKa = 3.87 KK201 pKa = 9.35 TGEE204 pKa = 4.19 NNWTASFSNATLSSDD219 pKa = 3.43 SSVTSGTVTGSIDD232 pKa = 3.26 IVFNEE237 pKa = 4.69 DD238 pKa = 2.58 GTLASTSPSPATISITGWTTGAANQTITLDD268 pKa = 3.41 LGTVGGADD276 pKa = 3.69 GLTQYY281 pKa = 11.18 SSDD284 pKa = 3.7 SQTPSIDD291 pKa = 3.86 LKK293 pKa = 11.44 SIDD296 pKa = 4.24 QDD298 pKa = 3.43 GLAYY302 pKa = 10.42 GSLTGISVEE311 pKa = 4.4 DD312 pKa = 4.01 GGNVTATYY320 pKa = 10.97 SNGEE324 pKa = 4.0 TYY326 pKa = 10.54 VIYY329 pKa = 10.36 KK330 pKa = 9.75 VAVATFANADD340 pKa = 3.58 GLTTVSGGMYY350 pKa = 9.92 QEE352 pKa = 4.37 STKK355 pKa = 10.88 SGSATLHH362 pKa = 5.42 EE363 pKa = 5.5 AGLGGAGSIKK373 pKa = 10.25 GSKK376 pKa = 10.09 LEE378 pKa = 4.21 SSTADD383 pKa = 3.19 TTAEE387 pKa = 4.03 FSTMITAQQAYY398 pKa = 9.09 SAAAQVVSTVSEE410 pKa = 4.31 MYY412 pKa = 8.28 DD413 pKa = 3.0 TLMSSVRR420 pKa = 3.49
Molecular weight: 42.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.694
IPC_protein 3.706
Toseland 3.49
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.656
Rodwell 3.528
Grimsley 3.389
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.541
EMBOSS 3.656
Sillero 3.834
Patrickios 0.668
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A327KJX9|A0A327KJX9_9RHIZ Uncharacterized protein OS=Rhodoplanes elegans OX=29408 GN=CH338_14630 PE=4 SV=1
MM1 pKa = 7.63 ARR3 pKa = 11.84 PIGAVAVRR11 pKa = 11.84 AAALTPARR19 pKa = 11.84 LPVALATVAPPIVALLATLRR39 pKa = 11.84 TVAPIAAMVATAAAIGPIAARR60 pKa = 11.84 FGALRR65 pKa = 11.84 RR66 pKa = 11.84 RR67 pKa = 11.84 HH68 pKa = 5.08 GLAVRR73 pKa = 4.34
Molecular weight: 7.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.398
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.149
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5973
0
5973
1759521
19
2359
294.6
31.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.919 ± 0.053
0.83 ± 0.011
5.678 ± 0.025
5.253 ± 0.024
3.444 ± 0.02
8.744 ± 0.034
1.953 ± 0.017
4.673 ± 0.023
2.978 ± 0.027
9.963 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.222 ± 0.016
2.076 ± 0.017
5.745 ± 0.027
2.701 ± 0.018
7.793 ± 0.034
4.789 ± 0.025
5.657 ± 0.027
8.303 ± 0.03
1.308 ± 0.013
1.971 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here