Pectobacterium phage CX5-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Phimunavirus; unclassified Phimunavirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D3P3|A0A5P8D3P3_9CAUD Uncharacterized protein OS=Pectobacterium phage CX5-1 OX=2652427 PE=4 SV=1
MM1 pKa = 7.0GTILLVLTLCSPSTGDD17 pKa = 3.36CDD19 pKa = 4.35AFIMDD24 pKa = 5.0TFNGDD29 pKa = 3.41EE30 pKa = 4.07VSKK33 pKa = 10.76CDD35 pKa = 4.15EE36 pKa = 4.61IIIASGQKK44 pKa = 9.74GAFPLRR50 pKa = 11.84CEE52 pKa = 4.34VQQ54 pKa = 2.92

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D3X5|A0A5P8D3X5_9CAUD Uncharacterized protein OS=Pectobacterium phage CX5-1 OX=2652427 PE=4 SV=1
MM1 pKa = 7.18ITRR4 pKa = 11.84KK5 pKa = 7.61TFHH8 pKa = 6.91RR9 pKa = 11.84ALVSMCLYY17 pKa = 10.78GAGKK21 pKa = 9.39QALFKK26 pKa = 10.57YY27 pKa = 10.25CRR29 pKa = 11.84ATQVKK34 pKa = 9.29HH35 pKa = 5.5VPARR39 pKa = 11.84YY40 pKa = 9.25KK41 pKa = 10.54KK42 pKa = 10.26RR43 pKa = 11.84IASKK47 pKa = 10.19VRR49 pKa = 11.84QQIKK53 pKa = 10.94DD54 pKa = 3.18MTFGLRR60 pKa = 11.84YY61 pKa = 9.04GRR63 pKa = 11.84HH64 pKa = 5.08VEE66 pKa = 3.87SDD68 pKa = 3.38NLFLSS73 pKa = 4.14

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13813

42

1230

251.1

27.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.665 ± 0.398

1.064 ± 0.148

6.545 ± 0.286

4.974 ± 0.318

3.077 ± 0.154

7.594 ± 0.258

2.092 ± 0.184

4.402 ± 0.155

4.163 ± 0.303

8.796 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.903 ± 0.163

4.184 ± 0.273

3.975 ± 0.239

4.51 ± 0.338

5.893 ± 0.282

6.291 ± 0.355

6.907 ± 0.461

7.949 ± 0.255

1.289 ± 0.139

3.728 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski