Xanthomonas phage Tenjo
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A679KKI9|A0A679KKI9_9CAUD Uncharacterized protein OS=Xanthomonas phage Tenjo OX=2674974 PE=4 SV=1
MM1 pKa = 7.52 TRR3 pKa = 11.84 DD4 pKa = 2.94 QWLEE8 pKa = 3.7 KK9 pKa = 10.08 CAEE12 pKa = 4.11 RR13 pKa = 11.84 LRR15 pKa = 11.84 EE16 pKa = 3.98 KK17 pKa = 10.97 AGMEE21 pKa = 4.1 PGEE24 pKa = 4.1 AAAYY28 pKa = 10.32 AIMLHH33 pKa = 6.72 DD34 pKa = 4.18 NQVNDD39 pKa = 3.52 NGRR42 pKa = 11.84 EE43 pKa = 3.94 PGSWDD48 pKa = 4.56 DD49 pKa = 3.89 PTDD52 pKa = 3.51 TADD55 pKa = 3.6 EE56 pKa = 4.86 EE57 pKa = 4.15 ISYY60 pKa = 10.17 MSDD63 pKa = 4.33 DD64 pKa = 3.92 EE65 pKa = 7.09 DD66 pKa = 6.85 GDD68 pKa = 4.35 DD69 pKa = 4.18 EE70 pKa = 4.5 PP71 pKa = 6.33
Molecular weight: 8.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.221
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.567
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.884
Patrickios 3.528
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A679KLG0|A0A679KLG0_9CAUD Uncharacterized protein OS=Xanthomonas phage Tenjo OX=2674974 PE=4 SV=1
MM1 pKa = 7.29 KK2 pKa = 10.22 RR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 TITQAEE12 pKa = 4.01 AQALKK17 pKa = 10.65 RR18 pKa = 11.84 RR19 pKa = 11.84 VAKK22 pKa = 10.95 LEE24 pKa = 4.05 DD25 pKa = 3.67 DD26 pKa = 3.82 EE27 pKa = 4.35 EE28 pKa = 4.53 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 AAWVSDD37 pKa = 3.58 YY38 pKa = 11.15 PDD40 pKa = 3.49 GVNIATHH47 pKa = 7.14 DD48 pKa = 4.16 FPSSDD53 pKa = 3.45 SVVPTALRR61 pKa = 11.84 TARR64 pKa = 11.84 KK65 pKa = 9.03 LGHH68 pKa = 7.08 AIVAVDD74 pKa = 3.63 DD75 pKa = 3.79 GHH77 pKa = 7.16 RR78 pKa = 11.84 LRR80 pKa = 11.84 FYY82 pKa = 11.33 ALPLPKK88 pKa = 10.31 AA89 pKa = 3.83
Molecular weight: 10.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.281
IPC2_protein 9.458
IPC_protein 10.087
Toseland 10.54
ProMoST 10.189
Dawson 10.643
Bjellqvist 10.321
Wikipedia 10.833
Rodwell 10.847
Grimsley 10.687
Solomon 10.745
Lehninger 10.73
Nozaki 10.511
DTASelect 10.321
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.57
Patrickios 10.628
IPC_peptide 10.76
IPC2_peptide 8.946
IPC2.peptide.svr19 8.815
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
13431
37
2341
235.6
25.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.789 ± 0.757
0.774 ± 0.185
6.619 ± 0.271
5.941 ± 0.284
2.911 ± 0.181
8.361 ± 0.316
1.459 ± 0.134
4.155 ± 0.241
3.946 ± 0.283
7.81 ± 0.288
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.36 ± 0.125
3.12 ± 0.181
4.936 ± 0.32
4.996 ± 0.266
7.006 ± 0.321
4.832 ± 0.24
5.941 ± 0.286
7.036 ± 0.309
1.631 ± 0.15
2.375 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here