Morganella phage MmP1
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D1FNQ4|D1FNQ4_9CAUD Uncharacterized protein OS=Morganella phage MmP1 OX=526118 GN=MmP1_gp02 PE=4 SV=1
MM1 pKa = 6.63 TTFNEE6 pKa = 4.87 LYY8 pKa = 10.71 ASAKK12 pKa = 9.75 EE13 pKa = 3.97 DD14 pKa = 3.34 LEE16 pKa = 4.05 AAIRR20 pKa = 11.84 EE21 pKa = 4.2 LHH23 pKa = 5.75 VTDD26 pKa = 4.81 TDD28 pKa = 4.21 DD29 pKa = 5.31 DD30 pKa = 4.86 DD31 pKa = 4.43 IRR33 pKa = 11.84 DD34 pKa = 4.46 RR35 pKa = 11.84 IHH37 pKa = 7.25 EE38 pKa = 4.29 IADD41 pKa = 3.74 SNVPIYY47 pKa = 10.91 NGDD50 pKa = 3.45 VFAVFADD57 pKa = 3.97 GGISYY62 pKa = 10.79 EE63 pKa = 4.16 MDD65 pKa = 3.62 DD66 pKa = 3.42 SGLIEE71 pKa = 4.5 GVTDD75 pKa = 3.26 VLHH78 pKa = 6.55 ILRR81 pKa = 11.84 VRR83 pKa = 11.84 IYY85 pKa = 10.75 EE86 pKa = 3.93 EE87 pKa = 5.55 LINCLYY93 pKa = 11.01 GDD95 pKa = 5.23 LEE97 pKa = 4.91 DD98 pKa = 5.28 CVTAYY103 pKa = 10.75 VEE105 pKa = 4.11 EE106 pKa = 5.67 LEE108 pKa = 5.9 DD109 pKa = 3.56 EE110 pKa = 5.07 AEE112 pKa = 4.2 TCGYY116 pKa = 10.94 GDD118 pKa = 5.22 DD119 pKa = 4.1 EE120 pKa = 4.52
Molecular weight: 13.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.656
IPC_protein 3.656
Toseland 3.439
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.834
Wikipedia 3.567
Rodwell 3.478
Grimsley 3.35
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 3.961
Thurlkill 3.49
EMBOSS 3.579
Sillero 3.77
Patrickios 0.528
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|D1FNQ5|D1FNQ5_9CAUD DNA-directed RNA polymerase OS=Morganella phage MmP1 OX=526118 GN=MmP1_gp09 PE=3 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.58 LNRR5 pKa = 11.84 LRR7 pKa = 11.84 KK8 pKa = 8.85 NMRR11 pKa = 11.84 YY12 pKa = 7.58 TIAYY16 pKa = 8.05 NGSFKK21 pKa = 10.66 RR22 pKa = 11.84 FKK24 pKa = 10.76 KK25 pKa = 10.29 NLRR28 pKa = 11.84 ASPDD32 pKa = 3.24 KK33 pKa = 10.89 GLSRR37 pKa = 11.84 IPARR41 pKa = 11.84 KK42 pKa = 9.35 LKK44 pKa = 10.87 AEE46 pKa = 4.08 WEE48 pKa = 4.17 WAVFEE53 pKa = 4.4 MTCRR57 pKa = 11.84 QQRR60 pKa = 11.84 EE61 pKa = 4.11 DD62 pKa = 3.34 ARR64 pKa = 11.84 LITFGLLYY72 pKa = 10.78 GIDD75 pKa = 3.94 YY76 pKa = 10.67 AGEE79 pKa = 3.89 VHH81 pKa = 7.12
Molecular weight: 9.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.284
IPC2_protein 9.663
IPC_protein 10.087
Toseland 10.496
ProMoST 10.116
Dawson 10.613
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 11.008
Grimsley 10.672
Solomon 10.672
Lehninger 10.643
Nozaki 10.467
DTASelect 10.277
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.54
Patrickios 10.76
IPC_peptide 10.672
IPC2_peptide 9.033
IPC2.peptide.svr19 8.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
11891
35
1312
242.7
27.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.923 ± 0.528
0.883 ± 0.139
6.425 ± 0.235
7.367 ± 0.353
3.557 ± 0.19
7.291 ± 0.327
1.783 ± 0.181
5.307 ± 0.211
6.955 ± 0.309
7.964 ± 0.328
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.969 ± 0.191
4.676 ± 0.23
3.692 ± 0.211
3.667 ± 0.321
5.391 ± 0.197
5.904 ± 0.328
5.735 ± 0.273
6.358 ± 0.239
1.413 ± 0.197
3.742 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here