Amino acid dipepetide frequency for Pseudomonas sp. TTU2014-080ASC

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.933AlaAla: 11.933 ± 0.142	1.235AlaCys: 1.235 ± 0.035	5.7AlaAsp: 5.7 ± 0.071	7.266AlaGlu: 7.266 ± 0.092	3.673AlaPhe: 3.673 ± 0.056	8.347AlaGly: 8.347 ± 0.096	2.052AlaHis: 2.052 ± 0.035	5.193AlaIle: 5.193 ± 0.059	3.952AlaLys: 3.952 ± 0.081	12.956AlaLeu: 12.956 ± 0.139	2.765AlaMet: 2.765 ± 0.043	3.182AlaAsn: 3.182 ± 0.06	4.22AlaPro: 4.22 ± 0.072	5.125AlaGln: 5.125 ± 0.07	6.219AlaArg: 6.219 ± 0.075	6.038AlaSer: 6.038 ± 0.073	4.459AlaThr: 4.459 ± 0.068	7.275AlaVal: 7.275 ± 0.085	1.387AlaTrp: 1.387 ± 0.028	2.526AlaTyr: 2.526 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.056CysAla: 1.056 ± 0.028	0.149CysCys: 0.149 ± 0.01	0.524CysAsp: 0.524 ± 0.018	0.58CysGlu: 0.58 ± 0.023	0.332CysPhe: 0.332 ± 0.016	0.938CysGly: 0.938 ± 0.029	0.274CysHis: 0.274 ± 0.016	0.452CysIle: 0.452 ± 0.019	0.319CysLys: 0.319 ± 0.015	1.124CysLeu: 1.124 ± 0.036	0.248CysMet: 0.248 ± 0.013	0.323CysAsn: 0.323 ± 0.017	0.511CysPro: 0.511 ± 0.022	0.392CysGln: 0.392 ± 0.019	0.585CysArg: 0.585 ± 0.02	0.661CysSer: 0.661 ± 0.022	0.474CysThr: 0.474 ± 0.018	0.685CysVal: 0.685 ± 0.022	0.159CysTrp: 0.159 ± 0.011	0.261CysTyr: 0.261 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	5.392AspAla: 5.392 ± 0.077	0.514AspCys: 0.514 ± 0.022	2.724AspAsp: 2.724 ± 0.051	3.584AspGlu: 3.584 ± 0.055	2.039AspPhe: 2.039 ± 0.041	4.209AspGly: 4.209 ± 0.076	1.089AspHis: 1.089 ± 0.028	2.756AspIle: 2.756 ± 0.045	2.063AspLys: 2.063 ± 0.044	5.814AspLeu: 5.814 ± 0.073	1.264AspMet: 1.264 ± 0.034	1.649AspAsn: 1.649 ± 0.04	2.511AspPro: 2.511 ± 0.041	2.265AspGln: 2.265 ± 0.044	2.756AspArg: 2.756 ± 0.044	3.034AspSer: 3.034 ± 0.052	2.327AspThr: 2.327 ± 0.041	3.65AspVal: 3.65 ± 0.056	0.913AspTrp: 0.913 ± 0.026	1.738AspTyr: 1.738 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	6.528GluAla: 6.528 ± 0.083	0.477GluCys: 0.477 ± 0.021	2.696GluAsp: 2.696 ± 0.048	3.456GluGlu: 3.456 ± 0.058	1.932GluPhe: 1.932 ± 0.042	4.028GluGly: 4.028 ± 0.066	1.638GluHis: 1.638 ± 0.033	2.984GluIle: 2.984 ± 0.051	2.326GluLys: 2.326 ± 0.045	7.661GluLeu: 7.661 ± 0.1	1.459GluMet: 1.459 ± 0.031	1.726GluAsn: 1.726 ± 0.037	2.557GluPro: 2.557 ± 0.04	4.159GluGln: 4.159 ± 0.068	4.673GluArg: 4.673 ± 0.063	2.926GluSer: 2.926 ± 0.052	2.606GluThr: 2.606 ± 0.051	4.411GluVal: 4.411 ± 0.057	0.774GluTrp: 0.774 ± 0.022	1.334GluTyr: 1.334 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	3.996PheAla: 3.996 ± 0.063	0.441PheCys: 0.441 ± 0.016	2.318PheAsp: 2.318 ± 0.046	2.088PheGlu: 2.088 ± 0.038	1.427PhePhe: 1.427 ± 0.039	3.12PheGly: 3.12 ± 0.05	0.677PheHis: 0.677 ± 0.02	2.056PheIle: 2.056 ± 0.038	1.409PheLys: 1.409 ± 0.033	3.131PheLeu: 3.131 ± 0.055	0.885PheMet: 0.885 ± 0.03	1.531PheAsn: 1.531 ± 0.031	1.447PhePro: 1.447 ± 0.026	1.231PheGln: 1.231 ± 0.03	1.805PheArg: 1.805 ± 0.042	2.717PheSer: 2.717 ± 0.051	2.001PheThr: 2.001 ± 0.042	2.517PheVal: 2.517 ± 0.046	0.534PheTrp: 0.534 ± 0.022	1.106PheTyr: 1.106 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	6.817GlyAla: 6.817 ± 0.087	0.954GlyCys: 0.954 ± 0.032	3.71GlyAsp: 3.71 ± 0.057	4.731GlyGlu: 4.731 ± 0.066	3.262GlyPhe: 3.262 ± 0.061	5.759GlyGly: 5.759 ± 0.087	1.75GlyHis: 1.75 ± 0.033	4.298GlyIle: 4.298 ± 0.062	3.537GlyLys: 3.537 ± 0.065	9.038GlyLeu: 9.038 ± 0.088	2.204GlyMet: 2.204 ± 0.043	2.461GlyAsn: 2.461 ± 0.059	2.417GlyPro: 2.417 ± 0.041	3.524GlyGln: 3.524 ± 0.053	4.513GlyArg: 4.513 ± 0.056	4.607GlySer: 4.607 ± 0.067	3.606GlyThr: 3.606 ± 0.076	5.749GlyVal: 5.749 ± 0.08	1.297GlyTrp: 1.297 ± 0.034	2.482GlyTyr: 2.482 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.109HisAla: 2.109 ± 0.043	0.309HisCys: 0.309 ± 0.015	1.106HisAsp: 1.106 ± 0.032	1.217HisGlu: 1.217 ± 0.033	0.974HisPhe: 0.974 ± 0.026	1.774HisGly: 1.774 ± 0.041	0.593HisHis: 0.593 ± 0.022	1.071HisIle: 1.071 ± 0.026	0.723HisLys: 0.723 ± 0.022	2.586HisLeu: 2.586 ± 0.052	0.506HisMet: 0.506 ± 0.022	0.734HisAsn: 0.734 ± 0.023	1.368HisPro: 1.368 ± 0.037	0.95HisGln: 0.95 ± 0.024	1.174HisArg: 1.174 ± 0.031	1.374HisSer: 1.374 ± 0.034	1.011HisThr: 1.011 ± 0.027	1.321HisVal: 1.321 ± 0.03	0.424HisTrp: 0.424 ± 0.019	0.783HisTyr: 0.783 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.818IleAla: 5.818 ± 0.069	0.553IleCys: 0.553 ± 0.02	3.198IleAsp: 3.198 ± 0.053	3.523IleGlu: 3.523 ± 0.054	1.608IlePhe: 1.608 ± 0.038	4.468IleGly: 4.468 ± 0.067	0.983IleHis: 0.983 ± 0.027	2.26IleIle: 2.26 ± 0.047	1.988IleLys: 1.988 ± 0.047	4.474IleLeu: 4.474 ± 0.063	0.906IleMet: 0.906 ± 0.029	2.04IleAsn: 2.04 ± 0.042	2.383IlePro: 2.383 ± 0.041	1.771IleGln: 1.771 ± 0.041	3.086IleArg: 3.086 ± 0.054	3.487IleSer: 3.487 ± 0.05	2.698IleThr: 2.698 ± 0.039	3.212IleVal: 3.212 ± 0.059	0.55IleTrp: 0.55 ± 0.021	1.214IleTyr: 1.214 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	4.217LysAla: 4.217 ± 0.075	0.192LysCys: 0.192 ± 0.014	1.863LysAsp: 1.863 ± 0.043	1.909LysGlu: 1.909 ± 0.042	0.938LysPhe: 0.938 ± 0.027	2.812LysGly: 2.812 ± 0.049	0.808LysHis: 0.808 ± 0.025	1.711LysIle: 1.711 ± 0.041	1.429LysLys: 1.429 ± 0.041	4.111LysLeu: 4.111 ± 0.058	0.8LysMet: 0.8 ± 0.027	1.195LysAsn: 1.195 ± 0.032	2.313LysPro: 2.313 ± 0.054	1.823LysGln: 1.823 ± 0.039	2.598LysArg: 2.598 ± 0.044	2.029LysSer: 2.029 ± 0.037	1.991LysThr: 1.991 ± 0.044	3.002LysVal: 3.002 ± 0.056	0.389LysTrp: 0.389 ± 0.018	0.751LysTyr: 0.751 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.207LeuAla: 13.207 ± 0.121	1.225LeuCys: 1.225 ± 0.031	6.405LeuAsp: 6.405 ± 0.074	6.73LeuGlu: 6.73 ± 0.077	4.197LeuPhe: 4.197 ± 0.065	8.665LeuGly: 8.665 ± 0.09	2.486LeuHis: 2.486 ± 0.049	6.178LeuIle: 6.178 ± 0.072	4.669LeuLys: 4.669 ± 0.064	13.674LeuLeu: 13.674 ± 0.162	2.651LeuMet: 2.651 ± 0.051	4.269LeuAsn: 4.269 ± 0.06	6.189LeuPro: 6.189 ± 0.074	5.014LeuGln: 5.014 ± 0.075	7.059LeuArg: 7.059 ± 0.081	8.068LeuSer: 8.068 ± 0.088	5.788LeuThr: 5.788 ± 0.088	7.557LeuVal: 7.557 ± 0.101	1.411LeuTrp: 1.411 ± 0.038	2.698LeuTyr: 2.698 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.549MetAla: 2.549 ± 0.048	0.18MetCys: 0.18 ± 0.013	1.087MetAsp: 1.087 ± 0.032	1.043MetGlu: 1.043 ± 0.027	0.757MetPhe: 0.757 ± 0.024	1.734MetGly: 1.734 ± 0.04	0.502MetHis: 0.502 ± 0.019	1.144MetIle: 1.144 ± 0.03	0.908MetLys: 0.908 ± 0.027	2.805MetLeu: 2.805 ± 0.05	0.511MetMet: 0.511 ± 0.023	0.883MetAsn: 0.883 ± 0.025	1.353MetPro: 1.353 ± 0.032	1.189MetGln: 1.189 ± 0.031	1.539MetArg: 1.539 ± 0.034	1.899MetSer: 1.899 ± 0.04	1.433MetThr: 1.433 ± 0.034	1.574MetVal: 1.574 ± 0.035	0.198MetTrp: 0.198 ± 0.012	0.423MetTyr: 0.423 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.321AsnAla: 3.321 ± 0.052	0.316AsnCys: 0.316 ± 0.014	1.682AsnAsp: 1.682 ± 0.039	1.735AsnGlu: 1.735 ± 0.036	1.183AsnPhe: 1.183 ± 0.033	2.741AsnGly: 2.741 ± 0.054	0.695AsnHis: 0.695 ± 0.023	1.659AsnIle: 1.659 ± 0.043	1.132AsnLys: 1.132 ± 0.029	3.85AsnLeu: 3.85 ± 0.058	0.674AsnMet: 0.674 ± 0.022	1.177AsnAsn: 1.177 ± 0.034	2.174AsnPro: 2.174 ± 0.044	1.504AsnGln: 1.504 ± 0.035	1.953AsnArg: 1.953 ± 0.038	1.983AsnSer: 1.983 ± 0.041	1.632AsnThr: 1.632 ± 0.041	2.071AsnVal: 2.071 ± 0.044	0.568AsnTrp: 0.568 ± 0.021	0.924AsnTyr: 0.924 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	5.183ProAla: 5.183 ± 0.088	0.351ProCys: 0.351 ± 0.017	2.732ProAsp: 2.732 ± 0.054	3.548ProGlu: 3.548 ± 0.057	1.752ProPhe: 1.752 ± 0.036	3.648ProGly: 3.648 ± 0.054	1.044ProHis: 1.044 ± 0.027	1.991ProIle: 1.991 ± 0.037	1.673ProLys: 1.673 ± 0.039	5.483ProLeu: 5.483 ± 0.067	1.135ProMet: 1.135 ± 0.023	1.528ProAsn: 1.528 ± 0.034	1.663ProPro: 1.663 ± 0.043	2.317ProGln: 2.317 ± 0.046	2.352ProArg: 2.352 ± 0.047	2.533ProSer: 2.533 ± 0.046	2.12ProThr: 2.12 ± 0.04	3.833ProVal: 3.833 ± 0.053	0.693ProTrp: 0.693 ± 0.026	1.237ProTyr: 1.237 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	5.75GlnAla: 5.75 ± 0.083	0.338GlnCys: 0.338 ± 0.015	1.884GlnAsp: 1.884 ± 0.037	2.203GlnGlu: 2.203 ± 0.044	1.482GlnPhe: 1.482 ± 0.037	3.332GlnGly: 3.332 ± 0.05	1.252GlnHis: 1.252 ± 0.034	2.371GlnIle: 2.371 ± 0.052	1.347GlnLys: 1.347 ± 0.032	5.979GlnLeu: 5.979 ± 0.082	1.086GlnMet: 1.086 ± 0.029	1.245GlnAsn: 1.245 ± 0.033	2.477GlnPro: 2.477 ± 0.046	3.041GlnGln: 3.041 ± 0.059	3.759GlnArg: 3.759 ± 0.06	2.511GlnSer: 2.511 ± 0.045	2.046GlnThr: 2.046 ± 0.042	3.608GlnVal: 3.608 ± 0.053	0.717GlnTrp: 0.717 ± 0.028	1.022GlnTyr: 1.022 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	5.454ArgAla: 5.454 ± 0.069	0.6ArgCys: 0.6 ± 0.022	3.251ArgAsp: 3.251 ± 0.055	4.311ArgGlu: 4.311 ± 0.065	2.646ArgPhe: 2.646 ± 0.05	3.696ArgGly: 3.696 ± 0.05	1.514ArgHis: 1.514 ± 0.033	3.275ArgIle: 3.275 ± 0.052	2.205ArgLys: 2.205 ± 0.038	7.999ArgLeu: 7.999 ± 0.098	1.62ArgMet: 1.62 ± 0.037	2.11ArgAsn: 2.11 ± 0.038	2.508ArgPro: 2.508 ± 0.043	3.135ArgGln: 3.135 ± 0.048	3.667ArgArg: 3.667 ± 0.061	3.505ArgSer: 3.505 ± 0.056	2.611ArgThr: 2.611 ± 0.046	4.17ArgVal: 4.17 ± 0.061	1.001ArgTrp: 1.001 ± 0.029	2.074ArgTyr: 2.074 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.28SerAla: 6.28 ± 0.063	0.548SerCys: 0.548 ± 0.023	2.95SerAsp: 2.95 ± 0.055	3.486SerGlu: 3.486 ± 0.048	2.337SerPhe: 2.337 ± 0.046	5.36SerGly: 5.36 ± 0.081	1.371SerHis: 1.371 ± 0.029	3.047SerIle: 3.047 ± 0.052	2.126SerLys: 2.126 ± 0.041	7.382SerLeu: 7.382 ± 0.072	1.493SerMet: 1.493 ± 0.035	2.035SerAsn: 2.035 ± 0.042	2.629SerPro: 2.629 ± 0.044	2.693SerGln: 2.693 ± 0.049	3.607SerArg: 3.607 ± 0.054	3.886SerSer: 3.886 ± 0.065	2.972SerThr: 2.972 ± 0.052	4.196SerVal: 4.196 ± 0.054	0.902SerTrp: 0.902 ± 0.028	1.581SerTyr: 1.581 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	4.948ThrAla: 4.948 ± 0.075	0.474ThrCys: 0.474 ± 0.02	2.497ThrAsp: 2.497 ± 0.045	2.635ThrGlu: 2.635 ± 0.051	1.76ThrPhe: 1.76 ± 0.037	4.065ThrGly: 4.065 ± 0.07	1.084ThrHis: 1.084 ± 0.03	2.055ThrIle: 2.055 ± 0.047	1.217ThrLys: 1.217 ± 0.035	6.501ThrLeu: 6.501 ± 0.077	0.806ThrMet: 0.806 ± 0.025	1.311ThrAsn: 1.311 ± 0.034	2.935ThrPro: 2.935 ± 0.05	2.096ThrGln: 2.096 ± 0.042	2.862ThrArg: 2.862 ± 0.05	2.688ThrSer: 2.688 ± 0.052	2.452ThrThr: 2.452 ± 0.056	3.347ThrVal: 3.347 ± 0.079	0.638ThrTrp: 0.638 ± 0.024	1.29ThrTyr: 1.29 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	7.323ValAla: 7.323 ± 0.085	0.717ValCys: 0.717 ± 0.021	3.928ValAsp: 3.928 ± 0.057	4.418ValGlu: 4.418 ± 0.063	2.577ValPhe: 2.577 ± 0.054	5.0ValGly: 5.0 ± 0.07	1.35ValHis: 1.35 ± 0.031	3.95ValIle: 3.95 ± 0.059	2.489ValLys: 2.489 ± 0.048	8.205ValLeu: 8.205 ± 0.095	1.822ValMet: 1.822 ± 0.041	2.288ValAsn: 2.288 ± 0.043	3.162ValPro: 3.162 ± 0.05	2.856ValGln: 2.856 ± 0.046	4.21ValArg: 4.21 ± 0.053	4.411ValSer: 4.411 ± 0.059	3.591ValThr: 3.591 ± 0.071	5.435ValVal: 5.435 ± 0.079	0.874ValTrp: 0.874 ± 0.025	1.607ValTyr: 1.607 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.116TrpAla: 1.116 ± 0.03	0.15TrpCys: 0.15 ± 0.01	0.646TrpAsp: 0.646 ± 0.022	0.586TrpGlu: 0.586 ± 0.022	0.539TrpPhe: 0.539 ± 0.019	0.903TrpGly: 0.903 ± 0.032	0.383TrpHis: 0.383 ± 0.017	0.653TrpIle: 0.653 ± 0.022	0.486TrpLys: 0.486 ± 0.021	2.207TrpLeu: 2.207 ± 0.049	0.374TrpMet: 0.374 ± 0.018	0.462TrpAsn: 0.462 ± 0.02	0.661TrpPro: 0.661 ± 0.022	0.968TrpGln: 0.968 ± 0.025	1.029TrpArg: 1.029 ± 0.032	0.821TrpSer: 0.821 ± 0.028	0.574TrpThr: 0.574 ± 0.021	0.962TrpVal: 0.962 ± 0.031	0.225TrpTrp: 0.225 ± 0.013	0.341TrpTyr: 0.341 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.507TyrAla: 2.507 ± 0.044	0.325TyrCys: 0.325 ± 0.015	1.389TyrAsp: 1.389 ± 0.04	1.299TyrGlu: 1.299 ± 0.035	1.022TyrPhe: 1.022 ± 0.027	2.114TyrGly: 2.114 ± 0.038	0.568TyrHis: 0.568 ± 0.021	1.115TyrIle: 1.115 ± 0.033	0.883TyrLys: 0.883 ± 0.029	3.159TyrLeu: 3.159 ± 0.046	0.505TyrMet: 0.505 ± 0.023	0.826TyrAsn: 0.826 ± 0.025	1.338TyrPro: 1.338 ± 0.028	1.395TyrGln: 1.395 ± 0.035	1.907TyrArg: 1.907 ± 0.036	1.704TyrSer: 1.704 ± 0.035	1.271TyrThr: 1.271 ± 0.035	1.633TyrVal: 1.633 ± 0.034	0.433TyrTrp: 0.433 ± 0.019	0.766TyrTyr: 0.766 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4032 proteins (1330008 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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