Paspalum dilatatum striate mosaic virus
Average proteome isoelectric point is 7.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7FHL5|J7FHL5_9GEMI Capsid protein OS=Paspalum dilatatum striate mosaic virus OX=1196236 PE=3 SV=1
MM1 pKa = 7.72 AEE3 pKa = 4.14 YY4 pKa = 9.93 PEE6 pKa = 4.5 SALVVSGAIPRR17 pKa = 11.84 RR18 pKa = 11.84 SQDD21 pKa = 2.85 EE22 pKa = 4.34 SFASSLKK29 pKa = 9.1 VTALSLFAAFIAACVLSFIYY49 pKa = 8.53 KK50 pKa = 7.87 TCIAEE55 pKa = 5.06 FITQYY60 pKa = 10.79 RR61 pKa = 11.84 LSGLSSVTSSGFGRR75 pKa = 11.84 TVEE78 pKa = 4.81 DD79 pKa = 4.25 PPAVPRR85 pKa = 11.84 TASEE89 pKa = 3.87 VSIPVAVRR97 pKa = 11.84 SGIPPVISGPSGIPGNN113 pKa = 3.95
Molecular weight: 11.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.719
IPC2_protein 6.046
IPC_protein 6.237
Toseland 5.842
ProMoST 6.237
Dawson 6.465
Bjellqvist 6.16
Wikipedia 6.338
Rodwell 6.389
Grimsley 5.753
Solomon 6.561
Lehninger 6.547
Nozaki 6.376
DTASelect 6.547
Thurlkill 6.44
EMBOSS 6.517
Sillero 6.693
Patrickios 4.101
IPC_peptide 6.561
IPC2_peptide 6.44
IPC2.peptide.svr19 6.513
Protein with the highest isoelectric point:
>tr|J7FHL5|J7FHL5_9GEMI Capsid protein OS=Paspalum dilatatum striate mosaic virus OX=1196236 PE=3 SV=1
MM1 pKa = 7.64 PSSSKK6 pKa = 10.36 RR7 pKa = 11.84 KK8 pKa = 9.43 RR9 pKa = 11.84 GSTSGTKK16 pKa = 9.53 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 9.75 KK20 pKa = 10.17 PRR22 pKa = 11.84 TTKK25 pKa = 8.29 WTGSRR30 pKa = 11.84 SASRR34 pKa = 11.84 EE35 pKa = 3.83 ALQVQTFTWSEE46 pKa = 3.89 DD47 pKa = 3.32 QAFNAGGRR55 pKa = 11.84 AILLSAYY62 pKa = 8.97 TRR64 pKa = 11.84 GSGEE68 pKa = 4.04 NQRR71 pKa = 11.84 KK72 pKa = 7.52 SQEE75 pKa = 4.02 TITYY79 pKa = 9.71 KK80 pKa = 10.51 VALNLGVSASATVLKK95 pKa = 9.71 YY96 pKa = 10.56 CCSSQPICWLVYY108 pKa = 10.45 DD109 pKa = 4.53 KK110 pKa = 10.89 TPTGITDD117 pKa = 4.22 LTPSDD122 pKa = 3.74 IFDD125 pKa = 3.86 VPSGLQNWPSTWKK138 pKa = 9.85 VKK140 pKa = 10.64 RR141 pKa = 11.84 EE142 pKa = 3.66 ASHH145 pKa = 6.57 RR146 pKa = 11.84 FVVKK150 pKa = 10.34 RR151 pKa = 11.84 RR152 pKa = 11.84 WPFKK156 pKa = 10.57 LEE158 pKa = 4.42 CNGSTFQKK166 pKa = 10.53 DD167 pKa = 3.78 YY168 pKa = 11.5 TNLPVCNTQNLVSVTRR184 pKa = 11.84 FAKK187 pKa = 10.25 GLGVRR192 pKa = 11.84 TEE194 pKa = 4.04 WKK196 pKa = 8.62 DD197 pKa = 3.19 TTTAEE202 pKa = 4.08 SSDD205 pKa = 3.35 IKK207 pKa = 11.11 GGALYY212 pKa = 10.55 LVIAPANGLVFTARR226 pKa = 11.84 GVIKK230 pKa = 10.39 CYY232 pKa = 10.02 FKK234 pKa = 11.2 SVGNQQ239 pKa = 2.8
Molecular weight: 26.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.203
IPC2_protein 9.414
IPC_protein 9.428
Toseland 10.277
ProMoST 9.853
Dawson 10.409
Bjellqvist 10.058
Wikipedia 10.54
Rodwell 10.935
Grimsley 10.452
Solomon 10.438
Lehninger 10.409
Nozaki 10.292
DTASelect 10.028
Thurlkill 10.277
EMBOSS 10.657
Sillero 10.321
Patrickios 10.628
IPC_peptide 10.438
IPC2_peptide 8.843
IPC2.peptide.svr19 8.371
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1030
113
366
257.5
28.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.155 ± 0.629
2.33 ± 0.197
3.981 ± 0.454
6.311 ± 1.012
4.66 ± 0.393
6.019 ± 0.537
2.233 ± 0.736
3.592 ± 0.736
6.699 ± 0.857
7.087 ± 0.444
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.359 ± 0.322
2.524 ± 0.565
6.408 ± 0.724
4.272 ± 0.46
6.117 ± 0.428
8.835 ± 1.457
6.117 ± 1.336
7.476 ± 0.368
1.942 ± 0.402
3.883 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here