Vagococcus humatus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S0AYP1|A0A3S0AYP1_9ENTE DNA translocase FtsK OS=Vagococcus humatus OX=1889241 GN=C7P63_03365 PE=4 SV=1
MM1 pKa = 7.42AVAKK5 pKa = 9.88IVYY8 pKa = 10.21ASLTGNTEE16 pKa = 3.93EE17 pKa = 4.62IADD20 pKa = 3.75IVAEE24 pKa = 4.0ALEE27 pKa = 4.6NYY29 pKa = 10.28DD30 pKa = 3.42IDD32 pKa = 4.5VEE34 pKa = 4.28QHH36 pKa = 5.57EE37 pKa = 4.83CTEE40 pKa = 3.81VDD42 pKa = 2.98ADD44 pKa = 3.87EE45 pKa = 6.04FEE47 pKa = 5.43DD48 pKa = 4.78ADD50 pKa = 4.03LCIVATYY57 pKa = 9.32TYY59 pKa = 11.29GEE61 pKa = 4.38GDD63 pKa = 4.04LPDD66 pKa = 4.95EE67 pKa = 4.37IVDD70 pKa = 4.39FYY72 pKa = 11.78EE73 pKa = 5.08DD74 pKa = 4.85LEE76 pKa = 4.12EE77 pKa = 5.91LDD79 pKa = 4.16LSGKK83 pKa = 9.8IFGVVGSGDD92 pKa = 3.17TFYY95 pKa = 11.49DD96 pKa = 4.45FFCKK100 pKa = 10.43SVDD103 pKa = 3.67DD104 pKa = 4.47FEE106 pKa = 4.86QAFLKK111 pKa = 10.21TGAKK115 pKa = 9.82KK116 pKa = 10.5GADD119 pKa = 3.41SVKK122 pKa = 10.17VDD124 pKa = 4.3LAAEE128 pKa = 4.17EE129 pKa = 4.62DD130 pKa = 4.46DD131 pKa = 4.32IEE133 pKa = 4.62HH134 pKa = 7.2LEE136 pKa = 4.21TFAKK140 pKa = 10.54SMAEE144 pKa = 3.78ALNAA148 pKa = 4.1

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A429Z4P5|A0A429Z4P5_9ENTE Energy-coupling factor transporter ATP-binding protein EcfA OS=Vagococcus humatus OX=1889241 GN=ecfA PE=3 SV=1
MM1 pKa = 7.13AQQRR5 pKa = 11.84RR6 pKa = 11.84GGRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.16VCYY16 pKa = 9.78FCANHH21 pKa = 6.86IDD23 pKa = 3.56HH24 pKa = 7.16VDD26 pKa = 3.66YY27 pKa = 11.43KK28 pKa = 11.32DD29 pKa = 3.83VDD31 pKa = 3.95LLKK34 pKa = 11.1NKK36 pKa = 9.32FVSEE40 pKa = 4.08RR41 pKa = 11.84GKK43 pKa = 9.89ILPRR47 pKa = 11.84RR48 pKa = 11.84VTGTCAKK55 pKa = 9.76HH56 pKa = 5.89QRR58 pKa = 11.84TLTIAIKK65 pKa = 10.14RR66 pKa = 11.84ARR68 pKa = 11.84IMGLLPFVADD78 pKa = 3.92EE79 pKa = 4.04QQ80 pKa = 3.97

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1964

0

1964

621691

23

1602

316.5

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.646 ± 0.053

0.745 ± 0.015

5.171 ± 0.043

7.266 ± 0.062

4.325 ± 0.039

6.625 ± 0.055

2.086 ± 0.029

7.405 ± 0.053

6.969 ± 0.046

10.237 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.737 ± 0.025

4.228 ± 0.034

3.65 ± 0.028

4.837 ± 0.054

3.59 ± 0.038

5.834 ± 0.04

5.91 ± 0.039

7.037 ± 0.047

0.899 ± 0.018

3.803 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski