Gordonia Phage Lollipop1437
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y6EKC7|A0A4Y6EKC7_9CAUD POU-specific domain-containing protein OS=Gordonia Phage Lollipop1437 OX=2588505 GN=72 PE=4 SV=1
MM1 pKa = 7.17 KK2 pKa = 9.87 TRR4 pKa = 11.84 IAIFLLAAVIFVFGPVAAALTGEE27 pKa = 4.52 AEE29 pKa = 4.19 ATPTKK34 pKa = 9.88 PCSISTATGSAPCPPPIISTNGDD57 pKa = 3.59 QIGGGANSEE66 pKa = 4.05 PVNMGEE72 pKa = 5.12 LPRR75 pKa = 11.84 TGPDD79 pKa = 3.21 YY80 pKa = 10.7 TYY82 pKa = 10.29 EE83 pKa = 4.11 APTYY87 pKa = 9.86 FDD89 pKa = 3.67 EE90 pKa = 5.4 PEE92 pKa = 4.55 APEE95 pKa = 4.68 APAEE99 pKa = 4.26 PEE101 pKa = 3.57 TDD103 pKa = 3.31 EE104 pKa = 4.5
Molecular weight: 10.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.812
IPC2_protein 3.973
IPC_protein 3.821
Toseland 3.668
ProMoST 3.897
Dawson 3.77
Bjellqvist 4.012
Wikipedia 3.656
Rodwell 3.656
Grimsley 3.579
Solomon 3.745
Lehninger 3.694
Nozaki 3.91
DTASelect 3.973
Thurlkill 3.694
EMBOSS 3.668
Sillero 3.923
Patrickios 1.85
IPC_peptide 3.757
IPC2_peptide 3.91
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A4Y6ETH5|A0A4Y6ETH5_9CAUD Uncharacterized protein OS=Gordonia Phage Lollipop1437 OX=2588505 GN=75 PE=4 SV=1
MM1 pKa = 7.42 TIVNRR6 pKa = 11.84 VPTLKK11 pKa = 10.38 PRR13 pKa = 11.84 GRR15 pKa = 11.84 CGFVRR20 pKa = 11.84 EE21 pKa = 4.35 SKK23 pKa = 10.44 LGAMLRR29 pKa = 11.84 CTLVAGHH36 pKa = 7.09 DD37 pKa = 3.67 EE38 pKa = 4.44 GRR40 pKa = 11.84 PHH42 pKa = 7.16 RR43 pKa = 11.84 PAGSPSRR50 pKa = 11.84 HH51 pKa = 4.99 YY52 pKa = 9.79 FAPLSDD58 pKa = 3.81 FDD60 pKa = 5.45 EE61 pKa = 4.64 NGQMIFKK68 pKa = 10.42 GRR70 pKa = 11.84 IINAPRR76 pKa = 11.84 DD77 pKa = 3.61 LYY79 pKa = 10.74 RR80 pKa = 11.84 GYY82 pKa = 10.0 IDD84 pKa = 3.28 PFEE87 pKa = 4.97 RR88 pKa = 11.84 IKK90 pKa = 10.97 ARR92 pKa = 11.84 QAAAEE97 pKa = 4.06 RR98 pKa = 11.84 VLRR101 pKa = 11.84 APLDD105 pKa = 3.88 FSHH108 pKa = 7.69 LDD110 pKa = 3.46 GLRR113 pKa = 11.84 KK114 pKa = 10.14 GGLLL118 pKa = 3.66
Molecular weight: 13.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.531
IPC_protein 10.379
Toseland 10.555
ProMoST 10.277
Dawson 10.657
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.804
Grimsley 10.716
Solomon 10.789
Lehninger 10.745
Nozaki 10.555
DTASelect 10.379
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.599
Patrickios 10.54
IPC_peptide 10.789
IPC2_peptide 9.487
IPC2.peptide.svr19 8.619
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
93
0
93
20929
37
1808
225.0
24.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.106 ± 0.411
0.674 ± 0.1
7.201 ± 0.312
6.68 ± 0.269
3.306 ± 0.14
8.744 ± 0.369
1.926 ± 0.163
5.046 ± 0.167
4.281 ± 0.277
7.578 ± 0.22
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.384 ± 0.112
3.283 ± 0.156
5.39 ± 0.167
3.077 ± 0.126
7.33 ± 0.352
5.213 ± 0.239
6.369 ± 0.221
6.947 ± 0.206
1.983 ± 0.13
2.485 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here