Amino acid dipepetide frequency for Streptomyces phage Dagobah

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.817AlaAla: 10.817 ± 1.125
1.202AlaCys: 1.202 ± 0.351
6.929AlaAsp: 6.929 ± 0.642
8.272AlaGlu: 8.272 ± 0.722
3.959AlaPhe: 3.959 ± 0.648
10.464AlaGly: 10.464 ± 1.016
1.768AlaHis: 1.768 ± 0.306
3.323AlaIle: 3.323 ± 0.58
3.182AlaLys: 3.182 ± 0.556
8.272AlaLeu: 8.272 ± 0.765
3.323AlaMet: 3.323 ± 0.483
2.545AlaAsn: 2.545 ± 0.484
5.656AlaPro: 5.656 ± 1.055
4.383AlaGln: 4.383 ± 0.531
6.717AlaArg: 6.717 ± 0.643
6.08AlaSer: 6.08 ± 0.804
5.868AlaThr: 5.868 ± 0.554
8.696AlaVal: 8.696 ± 0.728
2.262AlaTrp: 2.262 ± 0.391
1.768AlaTyr: 1.768 ± 0.361
0.0AlaXaa: 0.0 ± 0.0
Cys
0.848CysAla: 0.848 ± 0.248
0.071CysCys: 0.071 ± 0.073
0.707CysAsp: 0.707 ± 0.257
0.99CysGlu: 0.99 ± 0.269
0.283CysPhe: 0.283 ± 0.125
1.555CysGly: 1.555 ± 0.386
0.354CysHis: 0.354 ± 0.169
0.495CysIle: 0.495 ± 0.187
0.283CysLys: 0.283 ± 0.184
0.636CysLeu: 0.636 ± 0.224
0.141CysMet: 0.141 ± 0.11
0.141CysAsn: 0.141 ± 0.113
0.99CysPro: 0.99 ± 0.348
0.212CysGln: 0.212 ± 0.127
1.131CysArg: 1.131 ± 0.31
0.495CysSer: 0.495 ± 0.201
0.919CysThr: 0.919 ± 0.265
0.848CysVal: 0.848 ± 0.277
0.495CysTrp: 0.495 ± 0.177
0.424CysTyr: 0.424 ± 0.168
0.0CysXaa: 0.0 ± 0.0
Asp
7.353AspAla: 7.353 ± 0.778
0.636AspCys: 0.636 ± 0.202
4.666AspAsp: 4.666 ± 0.627
3.04AspGlu: 3.04 ± 0.376
1.98AspPhe: 1.98 ± 0.355
6.717AspGly: 6.717 ± 0.819
1.555AspHis: 1.555 ± 0.405
2.262AspIle: 2.262 ± 0.567
2.192AspLys: 2.192 ± 0.47
6.08AspLeu: 6.08 ± 0.559
1.273AspMet: 1.273 ± 0.313
1.98AspAsn: 1.98 ± 0.312
3.535AspPro: 3.535 ± 0.538
2.05AspGln: 2.05 ± 0.405
4.949AspArg: 4.949 ± 0.579
2.969AspSer: 2.969 ± 0.506
4.03AspThr: 4.03 ± 0.45
5.232AspVal: 5.232 ± 0.709
1.202AspTrp: 1.202 ± 0.312
1.697AspTyr: 1.697 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
6.434GluAla: 6.434 ± 0.82
0.848GluCys: 0.848 ± 0.27
4.878GluAsp: 4.878 ± 0.596
5.02GluGlu: 5.02 ± 0.739
1.768GluPhe: 1.768 ± 0.369
4.596GluGly: 4.596 ± 0.592
1.131GluHis: 1.131 ± 0.33
3.252GluIle: 3.252 ± 0.527
1.768GluLys: 1.768 ± 0.33
5.444GluLeu: 5.444 ± 0.931
1.626GluMet: 1.626 ± 0.321
2.262GluAsn: 2.262 ± 0.432
4.171GluPro: 4.171 ± 0.794
2.121GluGln: 2.121 ± 0.466
5.303GluArg: 5.303 ± 0.679
3.182GluSer: 3.182 ± 0.426
3.394GluThr: 3.394 ± 0.527
4.171GluVal: 4.171 ± 0.554
1.414GluTrp: 1.414 ± 0.316
1.909GluTyr: 1.909 ± 0.441
0.0GluXaa: 0.0 ± 0.0
Phe
2.616PheAla: 2.616 ± 0.395
0.283PheCys: 0.283 ± 0.147
2.616PheAsp: 2.616 ± 0.488
1.98PheGlu: 1.98 ± 0.384
0.636PhePhe: 0.636 ± 0.201
2.828PheGly: 2.828 ± 0.497
0.495PheHis: 0.495 ± 0.2
0.919PheIle: 0.919 ± 0.239
0.99PheLys: 0.99 ± 0.339
2.899PheLeu: 2.899 ± 0.459
0.566PheMet: 0.566 ± 0.249
0.848PheAsn: 0.848 ± 0.243
1.838PhePro: 1.838 ± 0.349
1.061PheGln: 1.061 ± 0.27
2.757PheArg: 2.757 ± 0.523
1.343PheSer: 1.343 ± 0.276
1.485PheThr: 1.485 ± 0.409
1.768PheVal: 1.768 ± 0.381
0.495PheTrp: 0.495 ± 0.239
0.848PheTyr: 0.848 ± 0.315
0.0PheXaa: 0.0 ± 0.0
Gly
8.555GlyAla: 8.555 ± 0.851
0.707GlyCys: 0.707 ± 0.194
6.363GlyAsp: 6.363 ± 0.863
6.505GlyGlu: 6.505 ± 0.684
2.545GlyPhe: 2.545 ± 0.441
7.706GlyGly: 7.706 ± 0.838
2.121GlyHis: 2.121 ± 0.409
2.828GlyIle: 2.828 ± 0.383
3.676GlyLys: 3.676 ± 0.625
6.999GlyLeu: 6.999 ± 0.711
1.838GlyMet: 1.838 ± 0.41
2.404GlyAsn: 2.404 ± 0.433
4.666GlyPro: 4.666 ± 0.62
3.676GlyGln: 3.676 ± 0.655
7.565GlyArg: 7.565 ± 0.79
5.373GlySer: 5.373 ± 0.714
6.434GlyThr: 6.434 ± 0.893
7.212GlyVal: 7.212 ± 0.593
2.192GlyTrp: 2.192 ± 0.379
2.969GlyTyr: 2.969 ± 0.439
0.0GlyXaa: 0.0 ± 0.0
His
2.333HisAla: 2.333 ± 0.419
0.212HisCys: 0.212 ± 0.114
1.202HisAsp: 1.202 ± 0.294
0.99HisGlu: 0.99 ± 0.279
0.495HisPhe: 0.495 ± 0.163
1.838HisGly: 1.838 ± 0.363
0.495HisHis: 0.495 ± 0.194
0.778HisIle: 0.778 ± 0.228
0.636HisLys: 0.636 ± 0.242
1.838HisLeu: 1.838 ± 0.34
0.071HisMet: 0.071 ± 0.07
0.636HisAsn: 0.636 ± 0.244
1.414HisPro: 1.414 ± 0.349
0.707HisGln: 0.707 ± 0.213
1.98HisArg: 1.98 ± 0.348
1.343HisSer: 1.343 ± 0.272
1.202HisThr: 1.202 ± 0.309
1.555HisVal: 1.555 ± 0.291
0.707HisTrp: 0.707 ± 0.214
0.707HisTyr: 0.707 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
4.101IleAla: 4.101 ± 0.423
0.707IleCys: 0.707 ± 0.217
2.899IleAsp: 2.899 ± 0.434
2.192IleGlu: 2.192 ± 0.452
0.636IlePhe: 0.636 ± 0.207
3.111IleGly: 3.111 ± 0.421
0.778IleHis: 0.778 ± 0.227
1.626IleIle: 1.626 ± 0.313
1.485IleLys: 1.485 ± 0.262
2.687IleLeu: 2.687 ± 0.396
0.566IleMet: 0.566 ± 0.212
0.99IleAsn: 0.99 ± 0.287
2.05IlePro: 2.05 ± 0.405
1.485IleGln: 1.485 ± 0.339
2.757IleArg: 2.757 ± 0.438
2.05IleSer: 2.05 ± 0.466
2.828IleThr: 2.828 ± 0.458
2.616IleVal: 2.616 ± 0.446
0.636IleTrp: 0.636 ± 0.216
0.707IleTyr: 0.707 ± 0.237
0.0IleXaa: 0.0 ± 0.0
Lys
3.252LysAla: 3.252 ± 0.568
0.283LysCys: 0.283 ± 0.156
1.768LysAsp: 1.768 ± 0.347
2.121LysGlu: 2.121 ± 0.57
0.495LysPhe: 0.495 ± 0.162
3.394LysGly: 3.394 ± 0.615
0.848LysHis: 0.848 ± 0.256
2.192LysIle: 2.192 ± 0.363
1.626LysLys: 1.626 ± 0.496
2.545LysLeu: 2.545 ± 0.512
0.919LysMet: 0.919 ± 0.259
0.919LysAsn: 0.919 ± 0.254
1.131LysPro: 1.131 ± 0.293
0.848LysGln: 0.848 ± 0.262
2.121LysArg: 2.121 ± 0.368
2.475LysSer: 2.475 ± 0.467
1.555LysThr: 1.555 ± 0.347
3.394LysVal: 3.394 ± 0.466
0.848LysTrp: 0.848 ± 0.253
0.707LysTyr: 0.707 ± 0.289
0.0LysXaa: 0.0 ± 0.0
Leu
8.696LeuAla: 8.696 ± 0.886
0.778LeuCys: 0.778 ± 0.205
5.303LeuAsp: 5.303 ± 0.673
5.515LeuGlu: 5.515 ± 0.778
2.121LeuPhe: 2.121 ± 0.381
7.141LeuGly: 7.141 ± 0.81
1.273LeuHis: 1.273 ± 0.318
2.828LeuIle: 2.828 ± 0.498
3.676LeuLys: 3.676 ± 0.568
6.434LeuLeu: 6.434 ± 0.775
2.192LeuMet: 2.192 ± 0.404
2.899LeuAsn: 2.899 ± 0.374
3.04LeuPro: 3.04 ± 0.383
1.555LeuGln: 1.555 ± 0.351
6.929LeuArg: 6.929 ± 0.644
4.242LeuSer: 4.242 ± 0.597
4.03LeuThr: 4.03 ± 0.494
5.868LeuVal: 5.868 ± 0.716
0.778LeuTrp: 0.778 ± 0.217
2.121LeuTyr: 2.121 ± 0.485
0.0LeuXaa: 0.0 ± 0.0
Met
2.545MetAla: 2.545 ± 0.31
0.354MetCys: 0.354 ± 0.147
1.131MetAsp: 1.131 ± 0.318
1.273MetGlu: 1.273 ± 0.34
0.495MetPhe: 0.495 ± 0.195
1.485MetGly: 1.485 ± 0.345
0.212MetHis: 0.212 ± 0.138
1.061MetIle: 1.061 ± 0.305
0.636MetLys: 0.636 ± 0.282
1.273MetLeu: 1.273 ± 0.269
0.283MetMet: 0.283 ± 0.144
0.566MetAsn: 0.566 ± 0.197
0.99MetPro: 0.99 ± 0.275
0.495MetGln: 0.495 ± 0.196
1.838MetArg: 1.838 ± 0.331
2.404MetSer: 2.404 ± 0.342
2.192MetThr: 2.192 ± 0.408
1.768MetVal: 1.768 ± 0.324
0.212MetTrp: 0.212 ± 0.125
0.495MetTyr: 0.495 ± 0.189
0.0MetXaa: 0.0 ± 0.0
Asn
3.04AsnAla: 3.04 ± 0.388
0.212AsnCys: 0.212 ± 0.153
1.697AsnAsp: 1.697 ± 0.31
1.768AsnGlu: 1.768 ± 0.338
1.061AsnPhe: 1.061 ± 0.329
3.182AsnGly: 3.182 ± 0.412
0.424AsnHis: 0.424 ± 0.153
1.061AsnIle: 1.061 ± 0.245
0.707AsnLys: 0.707 ± 0.196
1.768AsnLeu: 1.768 ± 0.359
0.848AsnMet: 0.848 ± 0.251
1.273AsnAsn: 1.273 ± 0.293
2.687AsnPro: 2.687 ± 0.524
0.919AsnGln: 0.919 ± 0.236
2.05AsnArg: 2.05 ± 0.387
1.485AsnSer: 1.485 ± 0.307
1.202AsnThr: 1.202 ± 0.288
1.838AsnVal: 1.838 ± 0.323
0.707AsnTrp: 0.707 ± 0.215
0.424AsnTyr: 0.424 ± 0.173
0.0AsnXaa: 0.0 ± 0.0
Pro
6.08ProAla: 6.08 ± 0.894
0.919ProCys: 0.919 ± 0.325
4.03ProAsp: 4.03 ± 0.46
4.383ProGlu: 4.383 ± 0.636
1.626ProPhe: 1.626 ± 0.358
5.444ProGly: 5.444 ± 0.587
0.778ProHis: 0.778 ± 0.219
1.131ProIle: 1.131 ± 0.262
1.273ProLys: 1.273 ± 0.323
3.394ProLeu: 3.394 ± 0.554
1.273ProMet: 1.273 ± 0.313
0.99ProAsn: 0.99 ± 0.272
2.192ProPro: 2.192 ± 0.337
2.404ProGln: 2.404 ± 0.482
3.606ProArg: 3.606 ± 0.611
3.747ProSer: 3.747 ± 0.564
3.394ProThr: 3.394 ± 0.433
5.444ProVal: 5.444 ± 0.692
0.99ProTrp: 0.99 ± 0.245
1.131ProTyr: 1.131 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
3.394GlnAla: 3.394 ± 0.438
0.354GlnCys: 0.354 ± 0.152
1.838GlnAsp: 1.838 ± 0.352
2.616GlnGlu: 2.616 ± 0.561
0.99GlnPhe: 0.99 ± 0.316
3.04GlnGly: 3.04 ± 0.51
0.99GlnHis: 0.99 ± 0.21
0.707GlnIle: 0.707 ± 0.245
0.99GlnLys: 0.99 ± 0.248
2.969GlnLeu: 2.969 ± 0.377
0.283GlnMet: 0.283 ± 0.121
0.778GlnAsn: 0.778 ± 0.269
1.838GlnPro: 1.838 ± 0.349
1.273GlnGln: 1.273 ± 0.325
2.757GlnArg: 2.757 ± 0.447
1.98GlnSer: 1.98 ± 0.374
1.414GlnThr: 1.414 ± 0.372
2.828GlnVal: 2.828 ± 0.411
0.848GlnTrp: 0.848 ± 0.239
1.626GlnTyr: 1.626 ± 0.342
0.0GlnXaa: 0.0 ± 0.0
Arg
7.919ArgAla: 7.919 ± 0.867
1.414ArgCys: 1.414 ± 0.277
3.464ArgAsp: 3.464 ± 0.479
4.383ArgGlu: 4.383 ± 0.528
2.404ArgPhe: 2.404 ± 0.512
5.798ArgGly: 5.798 ± 0.513
1.98ArgHis: 1.98 ± 0.337
3.818ArgIle: 3.818 ± 0.472
3.323ArgLys: 3.323 ± 0.514
5.939ArgLeu: 5.939 ± 0.617
2.262ArgMet: 2.262 ± 0.464
2.757ArgAsn: 2.757 ± 0.375
3.747ArgPro: 3.747 ± 0.5
2.545ArgGln: 2.545 ± 0.4
7.565ArgArg: 7.565 ± 1.345
4.454ArgSer: 4.454 ± 0.54
4.878ArgThr: 4.878 ± 0.695
5.868ArgVal: 5.868 ± 0.8
1.202ArgTrp: 1.202 ± 0.335
2.616ArgTyr: 2.616 ± 0.434
0.0ArgXaa: 0.0 ± 0.0
Ser
7.706SerAla: 7.706 ± 0.895
0.495SerCys: 0.495 ± 0.181
3.394SerAsp: 3.394 ± 0.706
2.687SerGlu: 2.687 ± 0.497
1.768SerPhe: 1.768 ± 0.523
7.353SerGly: 7.353 ± 0.823
0.99SerHis: 0.99 ± 0.249
2.687SerIle: 2.687 ± 0.497
1.273SerLys: 1.273 ± 0.332
3.747SerLeu: 3.747 ± 0.573
1.273SerMet: 1.273 ± 0.33
1.626SerAsn: 1.626 ± 0.26
3.818SerPro: 3.818 ± 0.467
1.768SerGln: 1.768 ± 0.43
4.171SerArg: 4.171 ± 0.562
3.889SerSer: 3.889 ± 0.566
4.03SerThr: 4.03 ± 0.689
3.959SerVal: 3.959 ± 0.609
1.485SerTrp: 1.485 ± 0.339
1.061SerTyr: 1.061 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
6.505ThrAla: 6.505 ± 0.789
0.707ThrCys: 0.707 ± 0.206
3.676ThrAsp: 3.676 ± 0.605
3.959ThrGlu: 3.959 ± 0.446
1.697ThrPhe: 1.697 ± 0.368
6.646ThrGly: 6.646 ± 0.623
1.414ThrHis: 1.414 ± 0.321
1.768ThrIle: 1.768 ± 0.388
1.768ThrLys: 1.768 ± 0.319
4.666ThrLeu: 4.666 ± 0.655
1.061ThrMet: 1.061 ± 0.283
0.919ThrAsn: 0.919 ± 0.263
4.171ThrPro: 4.171 ± 0.468
1.131ThrGln: 1.131 ± 0.348
4.454ThrArg: 4.454 ± 0.367
3.676ThrSer: 3.676 ± 0.606
4.101ThrThr: 4.101 ± 0.668
6.505ThrVal: 6.505 ± 0.788
1.414ThrTrp: 1.414 ± 0.329
1.909ThrTyr: 1.909 ± 0.359
0.0ThrXaa: 0.0 ± 0.0
Val
7.919ValAla: 7.919 ± 0.791
0.778ValCys: 0.778 ± 0.214
5.727ValAsp: 5.727 ± 0.587
4.596ValGlu: 4.596 ± 0.495
2.899ValPhe: 2.899 ± 0.436
6.01ValGly: 6.01 ± 0.748
2.475ValHis: 2.475 ± 0.486
2.687ValIle: 2.687 ± 0.465
2.616ValLys: 2.616 ± 0.406
5.585ValLeu: 5.585 ± 0.558
1.202ValMet: 1.202 ± 0.305
2.687ValAsn: 2.687 ± 0.439
4.03ValPro: 4.03 ± 0.47
2.616ValGln: 2.616 ± 0.423
6.01ValArg: 6.01 ± 0.718
5.02ValSer: 5.02 ± 0.64
5.798ValThr: 5.798 ± 0.584
5.585ValVal: 5.585 ± 0.635
1.697ValTrp: 1.697 ± 0.35
2.05ValTyr: 2.05 ± 0.337
0.0ValXaa: 0.0 ± 0.0
Trp
2.404TrpAla: 2.404 ± 0.4
0.566TrpCys: 0.566 ± 0.19
1.202TrpAsp: 1.202 ± 0.339
1.131TrpGlu: 1.131 ± 0.263
0.424TrpPhe: 0.424 ± 0.161
1.626TrpGly: 1.626 ± 0.388
0.495TrpHis: 0.495 ± 0.178
0.636TrpIle: 0.636 ± 0.207
0.778TrpLys: 0.778 ± 0.256
1.909TrpLeu: 1.909 ± 0.357
0.283TrpMet: 0.283 ± 0.133
0.495TrpAsn: 0.495 ± 0.191
0.636TrpPro: 0.636 ± 0.213
0.848TrpGln: 0.848 ± 0.254
1.485TrpArg: 1.485 ± 0.266
1.202TrpSer: 1.202 ± 0.27
1.414TrpThr: 1.414 ± 0.59
1.485TrpVal: 1.485 ± 0.291
0.212TrpTrp: 0.212 ± 0.122
0.848TrpTyr: 0.848 ± 0.242
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.969TyrAla: 2.969 ± 0.466
0.495TyrCys: 0.495 ± 0.197
1.909TyrAsp: 1.909 ± 0.286
1.273TyrGlu: 1.273 ± 0.287
0.99TyrPhe: 0.99 ± 0.272
2.404TyrGly: 2.404 ± 0.338
0.566TyrHis: 0.566 ± 0.202
0.848TyrIle: 0.848 ± 0.342
0.566TyrLys: 0.566 ± 0.198
2.333TyrLeu: 2.333 ± 0.409
0.354TyrMet: 0.354 ± 0.259
0.636TyrAsn: 0.636 ± 0.224
1.626TyrPro: 1.626 ± 0.356
1.414TyrGln: 1.414 ± 0.354
2.192TyrArg: 2.192 ± 0.335
1.768TyrSer: 1.768 ± 0.341
1.98TyrThr: 1.98 ± 0.441
1.343TyrVal: 1.343 ± 0.241
0.283TyrTrp: 0.283 ± 0.117
0.424TyrTyr: 0.424 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (14145 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski