Lactobacillus camelliae DSM 22697 = JCM 13995
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2347 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2F8E8|A0A0R2F8E8_9LACO 2-dehydro-3-deoxygluconokinase OS=Lactobacillus camelliae DSM 22697 = JCM 13995 OX=1423730 GN=FC75_GL001741 PE=4 SV=1
MM1 pKa = 6.4 MTSTLTIVGRR11 pKa = 11.84 EE12 pKa = 4.01 VFIDD16 pKa = 4.21 DD17 pKa = 3.89 YY18 pKa = 11.83 NEE20 pKa = 4.79 EE21 pKa = 3.78 IDD23 pKa = 3.53 NDD25 pKa = 3.71 YY26 pKa = 11.22 RR27 pKa = 11.84 LDD29 pKa = 4.06 PDD31 pKa = 5.31 EE32 pKa = 4.98 ILQDD36 pKa = 3.46 MVEE39 pKa = 4.34 LMEE42 pKa = 5.01 EE43 pKa = 4.27 SPEE46 pKa = 4.35 SYY48 pKa = 10.1 QHH50 pKa = 6.01 LHH52 pKa = 6.58 IDD54 pKa = 4.24 SEE56 pKa = 4.47 QTNDD60 pKa = 3.48 GMNKK64 pKa = 9.67 LFSFTSYY71 pKa = 10.41 EE72 pKa = 4.21 GEE74 pKa = 4.39 DD75 pKa = 3.91 GLRR78 pKa = 11.84 LSYY81 pKa = 10.93 LGVSDD86 pKa = 4.08 EE87 pKa = 4.24
Molecular weight: 10.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.719
IPC_protein 3.668
Toseland 3.478
ProMoST 3.808
Dawson 3.656
Bjellqvist 3.872
Wikipedia 3.579
Rodwell 3.503
Grimsley 3.389
Solomon 3.643
Lehninger 3.592
Nozaki 3.783
DTASelect 3.961
Thurlkill 3.528
EMBOSS 3.592
Sillero 3.795
Patrickios 1.825
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A0R2FL94|A0A0R2FL94_9LACO Polysaccharide transport membrane protein OS=Lactobacillus camelliae DSM 22697 = JCM 13995 OX=1423730 GN=FC75_GL002095 PE=4 SV=1
MM1 pKa = 5.72 TTKK4 pKa = 9.85 RR5 pKa = 11.84 TFQPKK10 pKa = 8.33 KK11 pKa = 7.5 RR12 pKa = 11.84 HH13 pKa = 5.56 RR14 pKa = 11.84 EE15 pKa = 3.79 RR16 pKa = 11.84 VHH18 pKa = 7.7 GFMKK22 pKa = 10.56 RR23 pKa = 11.84 MSTKK27 pKa = 10.26 NGRR30 pKa = 11.84 KK31 pKa = 8.58 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 9.94 GRR41 pKa = 11.84 KK42 pKa = 8.67 VLSAA46 pKa = 4.05
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2347
0
2347
732008
46
1761
311.9
34.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.435 ± 0.065
0.425 ± 0.011
5.965 ± 0.049
4.829 ± 0.046
4.014 ± 0.04
7.257 ± 0.049
2.26 ± 0.023
5.762 ± 0.049
5.403 ± 0.053
10.047 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.669 ± 0.026
3.792 ± 0.034
4.254 ± 0.034
4.679 ± 0.038
4.543 ± 0.041
5.285 ± 0.045
6.531 ± 0.049
7.363 ± 0.044
1.139 ± 0.024
3.336 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here