Amino acid dipepetide frequency for Anoxybacillus amylolyticus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.505AlaAla: 6.505 ± 0.115	0.734AlaCys: 0.734 ± 0.029	3.323AlaAsp: 3.323 ± 0.06	5.156AlaGlu: 5.156 ± 0.077	3.526AlaPhe: 3.526 ± 0.072	5.136AlaGly: 5.136 ± 0.094	1.638AlaHis: 1.638 ± 0.043	6.167AlaIle: 6.167 ± 0.091	5.839AlaLys: 5.839 ± 0.094	8.229AlaLeu: 8.229 ± 0.113	2.303AlaMet: 2.303 ± 0.054	3.028AlaAsn: 3.028 ± 0.056	2.329AlaPro: 2.329 ± 0.054	2.793AlaGln: 2.793 ± 0.051	3.443AlaArg: 3.443 ± 0.07	3.869AlaSer: 3.869 ± 0.07	4.021AlaThr: 4.021 ± 0.071	6.204AlaVal: 6.204 ± 0.095	0.675AlaTrp: 0.675 ± 0.027	2.637AlaTyr: 2.637 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.56CysAla: 0.56 ± 0.028	0.135CysCys: 0.135 ± 0.014	0.443CysAsp: 0.443 ± 0.023	0.594CysGlu: 0.594 ± 0.027	0.382CysPhe: 0.382 ± 0.019	0.758CysGly: 0.758 ± 0.029	0.262CysHis: 0.262 ± 0.016	0.541CysIle: 0.541 ± 0.026	0.354CysLys: 0.354 ± 0.019	0.775CysLeu: 0.775 ± 0.032	0.184CysMet: 0.184 ± 0.013	0.303CysAsn: 0.303 ± 0.019	0.445CysPro: 0.445 ± 0.025	0.305CysGln: 0.305 ± 0.02	0.38CysArg: 0.38 ± 0.023	0.508CysSer: 0.508 ± 0.023	0.45CysThr: 0.45 ± 0.023	0.546CysVal: 0.546 ± 0.026	0.088CysTrp: 0.088 ± 0.012	0.306CysTyr: 0.306 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.271AspAla: 3.271 ± 0.057	0.379AspCys: 0.379 ± 0.02	2.385AspAsp: 2.385 ± 0.06	4.368AspGlu: 4.368 ± 0.076	1.998AspPhe: 1.998 ± 0.05	3.371AspGly: 3.371 ± 0.064	1.037AspHis: 1.037 ± 0.04	3.832AspIle: 3.832 ± 0.065	2.26AspLys: 2.26 ± 0.057	4.171AspLeu: 4.171 ± 0.072	1.167AspMet: 1.167 ± 0.038	1.344AspAsn: 1.344 ± 0.04	1.914AspPro: 1.914 ± 0.047	1.444AspGln: 1.444 ± 0.04	2.394AspArg: 2.394 ± 0.056	1.879AspSer: 1.879 ± 0.05	2.11AspThr: 2.11 ± 0.05	4.384AspVal: 4.384 ± 0.072	0.64AspTrp: 0.64 ± 0.027	1.948AspTyr: 1.948 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	6.372GluAla: 6.372 ± 0.094	0.439GluCys: 0.439 ± 0.024	2.777GluAsp: 2.777 ± 0.059	6.887GluGlu: 6.887 ± 0.104	2.345GluPhe: 2.345 ± 0.052	4.119GluGly: 4.119 ± 0.077	1.832GluHis: 1.832 ± 0.043	5.056GluIle: 5.056 ± 0.073	6.953GluLys: 6.953 ± 0.099	7.115GluLeu: 7.115 ± 0.099	2.412GluMet: 2.412 ± 0.051	2.818GluAsn: 2.818 ± 0.062	1.999GluPro: 1.999 ± 0.05	4.802GluGln: 4.802 ± 0.087	4.746GluArg: 4.746 ± 0.085	2.628GluSer: 2.628 ± 0.056	4.221GluThr: 4.221 ± 0.074	5.009GluVal: 5.009 ± 0.08	0.996GluTrp: 0.996 ± 0.032	2.123GluTyr: 2.123 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	3.563PheAla: 3.563 ± 0.069	0.42PheCys: 0.42 ± 0.021	2.116PheAsp: 2.116 ± 0.053	2.38PheGlu: 2.38 ± 0.049	2.476PhePhe: 2.476 ± 0.064	3.221PheGly: 3.221 ± 0.059	1.189PheHis: 1.189 ± 0.036	3.226PheIle: 3.226 ± 0.07	1.66PheLys: 1.66 ± 0.049	4.695PheLeu: 4.695 ± 0.091	1.108PheMet: 1.108 ± 0.038	1.214PheAsn: 1.214 ± 0.036	1.88PhePro: 1.88 ± 0.045	1.721PheGln: 1.721 ± 0.049	2.015PheArg: 2.015 ± 0.052	3.118PheSer: 3.118 ± 0.065	2.465PheThr: 2.465 ± 0.056	3.481PheVal: 3.481 ± 0.064	0.509PheTrp: 0.509 ± 0.024	1.668PheTyr: 1.668 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.977GlyAla: 4.977 ± 0.089	0.668GlyCys: 0.668 ± 0.029	2.903GlyAsp: 2.903 ± 0.064	4.557GlyGlu: 4.557 ± 0.083	3.06GlyPhe: 3.06 ± 0.055	4.547GlyGly: 4.547 ± 0.089	1.41GlyHis: 1.41 ± 0.046	5.34GlyIle: 5.34 ± 0.078	5.219GlyLys: 5.219 ± 0.086	5.931GlyLeu: 5.931 ± 0.099	2.218GlyMet: 2.218 ± 0.056	2.392GlyAsn: 2.392 ± 0.053	1.542GlyPro: 1.542 ± 0.041	2.238GlyGln: 2.238 ± 0.048	2.966GlyArg: 2.966 ± 0.059	3.197GlySer: 3.197 ± 0.06	4.103GlyThr: 4.103 ± 0.076	5.356GlyVal: 5.356 ± 0.091	0.842GlyTrp: 0.842 ± 0.033	2.764GlyTyr: 2.764 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.608HisAla: 1.608 ± 0.046	0.265HisCys: 0.265 ± 0.019	1.164HisAsp: 1.164 ± 0.032	1.706HisGlu: 1.706 ± 0.045	1.059HisPhe: 1.059 ± 0.03	1.583HisGly: 1.583 ± 0.042	0.891HisHis: 0.891 ± 0.039	1.712HisIle: 1.712 ± 0.045	0.91HisLys: 0.91 ± 0.033	2.493HisLeu: 2.493 ± 0.058	0.595HisMet: 0.595 ± 0.024	0.616HisAsn: 0.616 ± 0.028	1.412HisPro: 1.412 ± 0.04	0.937HisGln: 0.937 ± 0.036	1.248HisArg: 1.248 ± 0.035	1.158HisSer: 1.158 ± 0.036	1.064HisThr: 1.064 ± 0.042	2.013HisVal: 2.013 ± 0.046	0.274HisTrp: 0.274 ± 0.019	0.986HisTyr: 0.986 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.44IleAla: 6.44 ± 0.094	0.656IleCys: 0.656 ± 0.025	4.183IleAsp: 4.183 ± 0.074	5.637IleGlu: 5.637 ± 0.078	2.829IlePhe: 2.829 ± 0.057	5.711IleGly: 5.711 ± 0.097	1.753IleHis: 1.753 ± 0.043	4.798IleIle: 4.798 ± 0.076	3.424IleLys: 3.424 ± 0.071	5.572IleLeu: 5.572 ± 0.081	1.401IleMet: 1.401 ± 0.042	2.307IleAsn: 2.307 ± 0.056	3.301IlePro: 3.301 ± 0.059	2.829IleGln: 2.829 ± 0.06	3.644IleArg: 3.644 ± 0.064	3.979IleSer: 3.979 ± 0.073	3.721IleThr: 3.721 ± 0.061	6.166IleVal: 6.166 ± 0.08	0.624IleTrp: 0.624 ± 0.027	2.349IleTyr: 2.349 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	5.105LysAla: 5.105 ± 0.093	0.356LysCys: 0.356 ± 0.022	2.881LysAsp: 2.881 ± 0.067	6.667LysGlu: 6.667 ± 0.096	1.67LysPhe: 1.67 ± 0.039	4.409LysGly: 4.409 ± 0.086	1.441LysHis: 1.441 ± 0.043	4.117LysIle: 4.117 ± 0.063	5.846LysLys: 5.846 ± 0.101	5.53LysLeu: 5.53 ± 0.081	2.158LysMet: 2.158 ± 0.054	2.536LysAsn: 2.536 ± 0.057	2.147LysPro: 2.147 ± 0.047	4.225LysGln: 4.225 ± 0.072	3.955LysArg: 3.955 ± 0.072	2.573LysSer: 2.573 ± 0.06	3.881LysThr: 3.881 ± 0.061	4.283LysVal: 4.283 ± 0.078	0.882LysTrp: 0.882 ± 0.033	2.064LysTyr: 2.064 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	8.439LeuAla: 8.439 ± 0.12	0.803LeuCys: 0.803 ± 0.033	4.52LeuAsp: 4.52 ± 0.069	6.698LeuGlu: 6.698 ± 0.097	4.971LeuPhe: 4.971 ± 0.093	6.039LeuGly: 6.039 ± 0.088	2.501LeuHis: 2.501 ± 0.058	5.916LeuIle: 5.916 ± 0.097	6.084LeuLys: 6.084 ± 0.083	10.756LeuLeu: 10.756 ± 0.129	2.271LeuMet: 2.271 ± 0.054	3.273LeuAsn: 3.273 ± 0.063	4.3LeuPro: 4.3 ± 0.056	4.322LeuGln: 4.322 ± 0.076	4.503LeuArg: 4.503 ± 0.081	6.259LeuSer: 6.259 ± 0.091	5.498LeuThr: 5.498 ± 0.083	6.853LeuVal: 6.853 ± 0.102	0.91LeuTrp: 0.91 ± 0.034	3.282LeuTyr: 3.282 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	2.134MetAla: 2.134 ± 0.052	0.169MetCys: 0.169 ± 0.014	1.205MetAsp: 1.205 ± 0.033	2.245MetGlu: 2.245 ± 0.049	1.075MetPhe: 1.075 ± 0.039	1.535MetGly: 1.535 ± 0.048	0.496MetHis: 0.496 ± 0.022	1.975MetIle: 1.975 ± 0.05	2.656MetLys: 2.656 ± 0.054	2.519MetLeu: 2.519 ± 0.051	1.042MetMet: 1.042 ± 0.035	1.456MetAsn: 1.456 ± 0.046	0.991MetPro: 0.991 ± 0.032	0.99MetGln: 0.99 ± 0.035	1.309MetArg: 1.309 ± 0.042	1.493MetSer: 1.493 ± 0.042	1.677MetThr: 1.677 ± 0.047	1.822MetVal: 1.822 ± 0.047	0.225MetTrp: 0.225 ± 0.016	0.827MetTyr: 0.827 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.6AsnAla: 2.6 ± 0.056	0.271AsnCys: 0.271 ± 0.016	2.032AsnAsp: 2.032 ± 0.052	3.355AsnGlu: 3.355 ± 0.061	1.243AsnPhe: 1.243 ± 0.04	3.118AsnGly: 3.118 ± 0.07	0.829AsnHis: 0.829 ± 0.027	2.834AsnIle: 2.834 ± 0.064	2.163AsnLys: 2.163 ± 0.051	2.607AsnLeu: 2.607 ± 0.062	1.015AsnMet: 1.015 ± 0.036	1.371AsnAsn: 1.371 ± 0.047	1.819AsnPro: 1.819 ± 0.052	1.292AsnGln: 1.292 ± 0.038	1.944AsnArg: 1.944 ± 0.041	1.46AsnSer: 1.46 ± 0.048	1.645AsnThr: 1.645 ± 0.045	2.973AsnVal: 2.973 ± 0.062	0.428AsnTrp: 0.428 ± 0.024	1.144AsnTyr: 1.144 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	2.418ProAla: 2.418 ± 0.052	0.264ProCys: 0.264 ± 0.017	1.843ProAsp: 1.843 ± 0.046	2.649ProGlu: 2.649 ± 0.06	2.214ProPhe: 2.214 ± 0.049	2.02ProGly: 2.02 ± 0.054	0.992ProHis: 0.992 ± 0.029	2.7ProIle: 2.7 ± 0.056	2.46ProLys: 2.46 ± 0.056	4.095ProLeu: 4.095 ± 0.071	0.886ProMet: 0.886 ± 0.032	1.697ProAsn: 1.697 ± 0.042	1.275ProPro: 1.275 ± 0.037	1.218ProGln: 1.218 ± 0.041	1.373ProArg: 1.373 ± 0.038	2.518ProSer: 2.518 ± 0.057	2.307ProThr: 2.307 ± 0.045	3.006ProVal: 3.006 ± 0.064	0.391ProTrp: 0.391 ± 0.02	1.588ProTyr: 1.588 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	3.565GlnAla: 3.565 ± 0.067	0.268GlnCys: 0.268 ± 0.018	1.266GlnAsp: 1.266 ± 0.042	2.985GlnGlu: 2.985 ± 0.061	1.773GlnPhe: 1.773 ± 0.04	2.147GlnGly: 2.147 ± 0.045	1.078GlnHis: 1.078 ± 0.037	2.566GlnIle: 2.566 ± 0.052	3.061GlnLys: 3.061 ± 0.079	5.105GlnLeu: 5.105 ± 0.093	1.358GlnMet: 1.358 ± 0.042	1.234GlnAsn: 1.234 ± 0.038	1.555GlnPro: 1.555 ± 0.041	2.531GlnGln: 2.531 ± 0.069	1.989GlnArg: 1.989 ± 0.052	1.934GlnSer: 1.934 ± 0.048	2.598GlnThr: 2.598 ± 0.069	2.437GlnVal: 2.437 ± 0.05	0.611GlnTrp: 0.611 ± 0.025	1.611GlnTyr: 1.611 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	3.295ArgAla: 3.295 ± 0.063	0.441ArgCys: 0.441 ± 0.026	2.236ArgAsp: 2.236 ± 0.053	3.794ArgGlu: 3.794 ± 0.077	2.352ArgPhe: 2.352 ± 0.053	2.714ArgGly: 2.714 ± 0.063	1.114ArgHis: 1.114 ± 0.039	3.343ArgIle: 3.343 ± 0.068	3.631ArgLys: 3.631 ± 0.079	5.259ArgLeu: 5.259 ± 0.088	1.574ArgMet: 1.574 ± 0.044	1.82ArgAsn: 1.82 ± 0.056	1.695ArgPro: 1.695 ± 0.044	2.221ArgGln: 2.221 ± 0.048	2.452ArgArg: 2.452 ± 0.059	2.365ArgSer: 2.365 ± 0.055	2.62ArgThr: 2.62 ± 0.058	3.262ArgVal: 3.262 ± 0.061	0.627ArgTrp: 0.627 ± 0.027	2.017ArgTyr: 2.017 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	3.494SerAla: 3.494 ± 0.058	0.413SerCys: 0.413 ± 0.024	2.399SerAsp: 2.399 ± 0.056	3.463SerGlu: 3.463 ± 0.072	3.146SerPhe: 3.146 ± 0.062	3.67SerGly: 3.67 ± 0.074	1.17SerHis: 1.17 ± 0.035	3.832SerIle: 3.832 ± 0.067	2.727SerLys: 2.727 ± 0.052	5.973SerLeu: 5.973 ± 0.093	1.449SerMet: 1.449 ± 0.041	1.74SerAsn: 1.74 ± 0.049	2.103SerPro: 2.103 ± 0.061	1.638SerGln: 1.638 ± 0.039	2.267SerArg: 2.267 ± 0.051	3.088SerSer: 3.088 ± 0.064	2.482SerThr: 2.482 ± 0.054	4.026SerVal: 4.026 ± 0.066	0.519SerTrp: 0.519 ± 0.028	1.942SerTyr: 1.942 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	3.982ThrAla: 3.982 ± 0.067	0.421ThrCys: 0.421 ± 0.024	2.467ThrAsp: 2.467 ± 0.051	3.554ThrGlu: 3.554 ± 0.062	2.687ThrPhe: 2.687 ± 0.055	3.996ThrGly: 3.996 ± 0.074	1.092ThrHis: 1.092 ± 0.036	4.635ThrIle: 4.635 ± 0.071	3.774ThrLys: 3.774 ± 0.064	5.331ThrLeu: 5.331 ± 0.077	1.495ThrMet: 1.495 ± 0.041	2.399ThrAsn: 2.399 ± 0.053	2.341ThrPro: 2.341 ± 0.045	1.33ThrGln: 1.33 ± 0.036	1.962ThrArg: 1.962 ± 0.052	2.88ThrSer: 2.88 ± 0.058	3.047ThrThr: 3.047 ± 0.067	4.715ThrVal: 4.715 ± 0.072	0.626ThrTrp: 0.626 ± 0.03	2.018ThrTyr: 2.018 ± 0.052	0.0ThrXaa: 0.0 ± 0.0
Val	6.15ValAla: 6.15 ± 0.093	0.768ValCys: 0.768 ± 0.03	3.691ValAsp: 3.691 ± 0.064	5.243ValGlu: 5.243 ± 0.077	3.123ValPhe: 3.123 ± 0.058	4.827ValGly: 4.827 ± 0.078	1.77ValHis: 1.77 ± 0.047	5.595ValIle: 5.595 ± 0.09	4.908ValLys: 4.908 ± 0.076	7.319ValLeu: 7.319 ± 0.093	1.938ValMet: 1.938 ± 0.046	2.769ValAsn: 2.769 ± 0.065	3.123ValPro: 3.123 ± 0.064	3.005ValGln: 3.005 ± 0.063	3.715ValArg: 3.715 ± 0.065	4.31ValSer: 4.31 ± 0.077	4.363ValThr: 4.363 ± 0.067	6.164ValVal: 6.164 ± 0.101	0.757ValTrp: 0.757 ± 0.03	2.666ValTyr: 2.666 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.633TrpAla: 0.633 ± 0.022	0.086TrpCys: 0.086 ± 0.011	0.489TrpAsp: 0.489 ± 0.025	0.745TrpGlu: 0.745 ± 0.029	0.478TrpPhe: 0.478 ± 0.024	0.699TrpGly: 0.699 ± 0.029	0.27TrpHis: 0.27 ± 0.017	0.79TrpIle: 0.79 ± 0.026	0.951TrpLys: 0.951 ± 0.035	1.439TrpLeu: 1.439 ± 0.042	0.386TrpMet: 0.386 ± 0.021	0.564TrpAsn: 0.564 ± 0.027	0.271TrpPro: 0.271 ± 0.018	0.512TrpGln: 0.512 ± 0.026	0.52TrpArg: 0.52 ± 0.027	0.531TrpSer: 0.531 ± 0.025	0.597TrpThr: 0.597 ± 0.025	0.723TrpVal: 0.723 ± 0.026	0.165TrpTrp: 0.165 ± 0.015	0.37TrpTyr: 0.37 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.446TyrAla: 2.446 ± 0.048	0.363TyrCys: 0.363 ± 0.02	1.972TyrAsp: 1.972 ± 0.054	2.966TyrGlu: 2.966 ± 0.062	1.717TyrPhe: 1.717 ± 0.049	2.612TyrGly: 2.612 ± 0.06	0.929TyrHis: 0.929 ± 0.034	2.349TyrIle: 2.349 ± 0.052	1.848TyrLys: 1.848 ± 0.047	3.284TyrLeu: 3.284 ± 0.064	0.88TyrMet: 0.88 ± 0.031	1.264TyrAsn: 1.264 ± 0.043	1.474TyrPro: 1.474 ± 0.048	1.32TyrGln: 1.32 ± 0.045	2.006TyrArg: 2.006 ± 0.044	1.793TyrSer: 1.793 ± 0.035	1.881TyrThr: 1.881 ± 0.042	2.779TyrVal: 2.779 ± 0.054	0.429TyrTrp: 0.429 ± 0.021	1.457TyrTyr: 1.457 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3186 proteins (897943 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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