Soybean cyst nematode socyvirus
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G0WXQ0|G0WXQ0_9MONO Uncharacterized protein OS=Soybean cyst nematode socyvirus OX=1034377 PE=4 SV=1
MM1 pKa = 7.38 SASQRR6 pKa = 11.84 GSTSQPSGSNGQPTPSPDD24 pKa = 3.29 RR25 pKa = 11.84 HH26 pKa = 5.59 WSSTPSLLNVLQSAVDD42 pKa = 3.72 RR43 pKa = 11.84 AATFQTTARR52 pKa = 11.84 PPPNPNSLAPPPVVVEE68 pKa = 4.7 GGSDD72 pKa = 3.45 MEE74 pKa = 4.81 PDD76 pKa = 3.43 EE77 pKa = 5.63 EE78 pKa = 5.29 EE79 pKa = 4.3 DD80 pKa = 4.18 TEE82 pKa = 4.34 HH83 pKa = 6.65 QNKK86 pKa = 9.31 GPNDD90 pKa = 3.84 DD91 pKa = 4.41 EE92 pKa = 4.75 VADD95 pKa = 4.03 SQEE98 pKa = 4.21 SGGNSDD104 pKa = 5.34 DD105 pKa = 4.63 HH106 pKa = 7.59 PDD108 pKa = 3.84 DD109 pKa = 5.97 AGDD112 pKa = 3.74 TNSAGLLTQLAAALAVPGTEE132 pKa = 3.96 HH133 pKa = 7.18 FMGTLAQDD141 pKa = 3.45 LHH143 pKa = 6.41 GLPSRR148 pKa = 11.84 EE149 pKa = 3.95 KK150 pKa = 10.8 DD151 pKa = 3.29 RR152 pKa = 11.84 LFAALLINIAYY163 pKa = 9.11 RR164 pKa = 11.84 QANPPPPPPAPSAIDD179 pKa = 3.53 PDD181 pKa = 3.65 LAARR185 pKa = 11.84 LNAMNIRR192 pKa = 11.84 IHH194 pKa = 5.06 QLQVKK199 pKa = 9.85 VGFDD203 pKa = 3.44 TTDD206 pKa = 3.37 PLANPPIPPVASYY219 pKa = 11.34 GGGLPFGSGPSNPDD233 pKa = 2.52 MTTAFAGYY241 pKa = 10.16 GRR243 pKa = 11.84 GSTTMSTAPKK253 pKa = 10.05 VPQTPEE259 pKa = 3.45 QTPEE263 pKa = 3.66 EE264 pKa = 4.34 KK265 pKa = 10.41 AKK267 pKa = 10.68 KK268 pKa = 9.96 VADD271 pKa = 3.96 KK272 pKa = 10.27 LAKK275 pKa = 9.49 MKK277 pKa = 10.45 NQNLL281 pKa = 3.62
Molecular weight: 29.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.746
IPC2_protein 4.647
IPC_protein 4.596
Toseland 4.444
ProMoST 4.736
Dawson 4.571
Bjellqvist 4.724
Wikipedia 4.482
Rodwell 4.444
Grimsley 4.355
Solomon 4.571
Lehninger 4.533
Nozaki 4.685
DTASelect 4.902
Thurlkill 4.457
EMBOSS 4.495
Sillero 4.736
Patrickios 4.228
IPC_peptide 4.571
IPC2_peptide 4.724
IPC2.peptide.svr19 4.673
Protein with the highest isoelectric point:
>tr|G0WXQ1|G0WXQ1_9MONO Glycoprotein OS=Soybean cyst nematode socyvirus OX=1034377 PE=4 SV=1
MM1 pKa = 7.85 SPMKK5 pKa = 10.59 SSLDD9 pKa = 3.11 PWRR12 pKa = 11.84 IRR14 pKa = 11.84 TSDD17 pKa = 3.49 RR18 pKa = 11.84 QALDD22 pKa = 3.34 PGYY25 pKa = 7.72 WTAEE29 pKa = 3.61 RR30 pKa = 11.84 RR31 pKa = 11.84 QLRR34 pKa = 11.84 DD35 pKa = 3.33 LLTSITVSRR44 pKa = 11.84 SPSPTSRR51 pKa = 11.84 PTATVGTQTDD61 pKa = 4.06 PAHH64 pKa = 6.55 CMCCGKK70 pKa = 9.96 VHH72 pKa = 6.78 LVTYY76 pKa = 9.99 KK77 pKa = 10.18 IVHH80 pKa = 6.21
Molecular weight: 9.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.223
IPC2_protein 8.887
IPC_protein 9.077
Toseland 9.472
ProMoST 9.341
Dawson 9.75
Bjellqvist 9.648
Wikipedia 9.926
Rodwell 9.911
Grimsley 9.794
Solomon 9.911
Lehninger 9.882
Nozaki 9.838
DTASelect 9.531
Thurlkill 9.633
EMBOSS 9.911
Sillero 9.809
Patrickios 7.585
IPC_peptide 9.897
IPC2_peptide 8.96
IPC2.peptide.svr19 7.69
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3420
80
2086
684.0
76.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.538 ± 1.754
1.901 ± 0.416
5.614 ± 0.329
5.234 ± 0.314
2.953 ± 0.528
6.023 ± 0.743
2.427 ± 0.135
5.351 ± 0.595
4.678 ± 0.391
10.731 ± 0.968
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.953 ± 0.266
3.187 ± 0.37
6.491 ± 1.016
3.713 ± 0.381
6.14 ± 0.838
6.404 ± 0.688
7.164 ± 0.402
5.906 ± 0.231
1.696 ± 0.271
2.895 ± 0.434
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here