Beihai sobemo-like virus 11
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEC8|A0A1L3KEC8_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 11 OX=1922682 PE=4 SV=1
MM1 pKa = 7.38 SVFAQSLGEE10 pKa = 3.88 RR11 pKa = 11.84 VMEE14 pKa = 4.64 LFGTFAGACYY24 pKa = 10.0 HH25 pKa = 5.81 SARR28 pKa = 11.84 KK29 pKa = 8.38 FAEE32 pKa = 4.11 AADD35 pKa = 3.91 YY36 pKa = 10.12 QLPSLASIIGYY47 pKa = 9.62 VLMGLTFCVAQSLWSWMSPSATKK70 pKa = 9.87 TFKK73 pKa = 10.75 QFLGACKK80 pKa = 10.22 ALWKK84 pKa = 10.45 YY85 pKa = 10.48 RR86 pKa = 11.84 ADD88 pKa = 3.93 RR89 pKa = 11.84 MQKK92 pKa = 9.94 PITGTVVGPVHH103 pKa = 7.41 GYY105 pKa = 6.84 FTHH108 pKa = 6.74 GAEE111 pKa = 3.84 HH112 pKa = 7.29 YY113 pKa = 8.38 YY114 pKa = 10.67 RR115 pKa = 11.84 CVKK118 pKa = 9.55 TSKK121 pKa = 10.61 LYY123 pKa = 10.08 IVSPGRR129 pKa = 11.84 GRR131 pKa = 11.84 FYY133 pKa = 10.29 IDD135 pKa = 3.05 KK136 pKa = 10.62 NGEE139 pKa = 3.88 EE140 pKa = 4.52 KK141 pKa = 11.32 YY142 pKa = 10.05 EE143 pKa = 4.01 VCDD146 pKa = 3.7 YY147 pKa = 11.22 VHH149 pKa = 6.99 EE150 pKa = 4.58 LVQSSGIEE158 pKa = 3.86 AEE160 pKa = 4.28 ANVHH164 pKa = 5.88 NGEE167 pKa = 4.23 PALPTPALDD176 pKa = 3.44 KK177 pKa = 11.21 CVVTLAFKK185 pKa = 10.69 DD186 pKa = 3.92 SNGDD190 pKa = 3.09 IHH192 pKa = 7.26 NLGHH196 pKa = 5.24 GHH198 pKa = 7.21 RR199 pKa = 11.84 MGDD202 pKa = 3.54 YY203 pKa = 10.53 LVTNAHH209 pKa = 6.01 VNDD212 pKa = 4.17 AGLARR217 pKa = 11.84 SEE219 pKa = 4.53 DD220 pKa = 3.7 GQIVFTKK227 pKa = 10.22 DD228 pKa = 2.9 NQKK231 pKa = 10.35 FYY233 pKa = 10.4 PAGEE237 pKa = 4.35 FIYY240 pKa = 8.77 FTKK243 pKa = 10.51 NYY245 pKa = 9.35 EE246 pKa = 4.09 KK247 pKa = 10.59 ATGNDD252 pKa = 2.79 LGAYY256 pKa = 9.08 KK257 pKa = 10.34 ISLGTWAASGVTSFQPSNYY276 pKa = 8.91 NPQGEE281 pKa = 4.24 GRR283 pKa = 11.84 IYY285 pKa = 9.79 LTAYY289 pKa = 10.42 DD290 pKa = 4.07 DD291 pKa = 4.12 VRR293 pKa = 11.84 GKK295 pKa = 9.36 TMMAKK300 pKa = 10.3 GYY302 pKa = 9.86 LHH304 pKa = 6.58 NQTKK308 pKa = 9.81 EE309 pKa = 3.75 DD310 pKa = 4.12 CKK312 pKa = 11.05 AGLIPHH318 pKa = 6.25 TAITRR323 pKa = 11.84 AGYY326 pKa = 10.14 SGTPIFLMKK335 pKa = 10.06 GQRR338 pKa = 11.84 RR339 pKa = 11.84 TMVGIHH345 pKa = 6.2 CGGRR349 pKa = 11.84 IDD351 pKa = 4.58 GKK353 pKa = 11.23 GNYY356 pKa = 8.79 GCSIYY361 pKa = 10.88 EE362 pKa = 3.87 LFQFRR367 pKa = 11.84 RR368 pKa = 11.84 QLGLEE373 pKa = 3.94 PKK375 pKa = 10.2 IPLICEE381 pKa = 4.15 AKK383 pKa = 10.56 YY384 pKa = 10.49 ISPPTKK390 pKa = 9.61 DD391 pKa = 3.09 RR392 pKa = 11.84 QYY394 pKa = 11.81 DD395 pKa = 3.55 RR396 pKa = 11.84 YY397 pKa = 10.75 DD398 pKa = 3.25 QEE400 pKa = 4.2 TPEE403 pKa = 3.9 QRR405 pKa = 11.84 EE406 pKa = 3.78 EE407 pKa = 4.21 RR408 pKa = 11.84 KK409 pKa = 10.42 DD410 pKa = 3.5 RR411 pKa = 11.84 EE412 pKa = 4.4 AMEE415 pKa = 4.27 KK416 pKa = 10.37 AAEE419 pKa = 3.98 EE420 pKa = 4.16 EE421 pKa = 4.02 IRR423 pKa = 11.84 EE424 pKa = 4.15 NLHH427 pKa = 6.58 GEE429 pKa = 4.09 ALVPEE434 pKa = 4.57 AALDD438 pKa = 4.0 GPTWVEE444 pKa = 3.67 LGVNTGFEE452 pKa = 3.84 IDD454 pKa = 3.26 MDD456 pKa = 4.08 EE457 pKa = 4.25 THH459 pKa = 6.25 SQSVGISDD467 pKa = 4.62 LGSVSDD473 pKa = 4.57 LGLSEE478 pKa = 6.43 DD479 pKa = 4.18 EE480 pKa = 4.32 VQLAAEE486 pKa = 4.0 QLPRR490 pKa = 11.84 ANLGLNEE497 pKa = 4.66 DD498 pKa = 4.46 ALQEE502 pKa = 3.89 LHH504 pKa = 6.52 QDD506 pKa = 2.86 QDD508 pKa = 3.43 EE509 pKa = 4.34 RR510 pKa = 11.84 RR511 pKa = 11.84 TAAVEE516 pKa = 3.79 RR517 pKa = 11.84 LRR519 pKa = 11.84 TYY521 pKa = 11.23 AEE523 pKa = 3.8 QQYY526 pKa = 9.58 EE527 pKa = 4.57 QIPEE531 pKa = 3.98 PAPPQEE537 pKa = 4.09 QEE539 pKa = 4.07 VMSAADD545 pKa = 4.49 KK546 pKa = 11.18 YY547 pKa = 11.7 DD548 pKa = 5.69 LIPDD552 pKa = 5.34 PIGEE556 pKa = 4.38 PEE558 pKa = 4.14 FEE560 pKa = 5.04 DD561 pKa = 5.25 GEE563 pKa = 4.29 IEE565 pKa = 4.0 EE566 pKa = 4.72 PMVHH570 pKa = 6.57 FPASGQPVVLGPQVSRR586 pKa = 11.84 EE587 pKa = 3.8 EE588 pKa = 4.19 KK589 pKa = 10.13 EE590 pKa = 3.7 RR591 pKa = 11.84 GEE593 pKa = 3.96 RR594 pKa = 11.84 TFASRR599 pKa = 11.84 ASMPLWVKK607 pKa = 10.54 GFLGVGLLQAANVVSPTGKK626 pKa = 7.89 TRR628 pKa = 11.84 EE629 pKa = 4.14 VTDD632 pKa = 3.79 EE633 pKa = 3.93 QVKK636 pKa = 10.8 DD637 pKa = 3.74 LLRR640 pKa = 11.84 CRR642 pKa = 11.84 PQDD645 pKa = 3.25 LAAARR650 pKa = 11.84 KK651 pKa = 9.38 LDD653 pKa = 3.97 PQSLLNSTPYY663 pKa = 10.09 EE664 pKa = 4.11 VYY666 pKa = 10.22 RR667 pKa = 11.84 SYY669 pKa = 11.2 LGCGDD674 pKa = 4.55 VEE676 pKa = 4.17 WTPDD680 pKa = 3.23 GEE682 pKa = 4.96 KK683 pKa = 10.45 IKK685 pKa = 10.79 DD686 pKa = 3.67 DD687 pKa = 3.68 QDD689 pKa = 3.3 NVLAARR695 pKa = 11.84 VGKK698 pKa = 10.39 SRR700 pKa = 11.84 MFSRR704 pKa = 11.84 SSNKK708 pKa = 9.46 SHH710 pKa = 7.42 LPDD713 pKa = 3.69 KK714 pKa = 9.92 YY715 pKa = 10.75 KK716 pKa = 10.86 KK717 pKa = 10.46 VVADD721 pKa = 4.8 LKK723 pKa = 10.98 LGPQQGFKK731 pKa = 10.56 DD732 pKa = 4.01 YY733 pKa = 11.43 VMPPTGPEE741 pKa = 3.64 AVLGSLRR748 pKa = 11.84 TQLKK752 pKa = 7.73 KK753 pKa = 9.48 TSVEE757 pKa = 3.79 PWPKK761 pKa = 10.57 EE762 pKa = 3.45 FLEE765 pKa = 4.74 RR766 pKa = 11.84 DD767 pKa = 3.62 GGDD770 pKa = 3.05 HH771 pKa = 6.98 LRR773 pKa = 11.84 NEE775 pKa = 4.38 LHH777 pKa = 7.5 DD778 pKa = 4.27 EE779 pKa = 4.07 ASKK782 pKa = 11.48 YY783 pKa = 9.27 PLAYY787 pKa = 9.57 EE788 pKa = 4.21 AGFLNVHH795 pKa = 6.41 DD796 pKa = 4.79 FVVKK800 pKa = 10.29 LAKK803 pKa = 10.5 EE804 pKa = 3.87 FDD806 pKa = 3.92 GTKK809 pKa = 10.04 SAGWSQLYY817 pKa = 10.02 RR818 pKa = 11.84 PGNKK822 pKa = 9.49 AVWQTEE828 pKa = 3.92 EE829 pKa = 4.03 GLALASYY836 pKa = 7.7 MVRR839 pKa = 11.84 CRR841 pKa = 11.84 LLLRR845 pKa = 11.84 MAWGAEE851 pKa = 3.93 AMSKK855 pKa = 9.64 MSPEE859 pKa = 3.8 QMFQNGLSDD868 pKa = 3.78 PKK870 pKa = 9.69 VASVKK875 pKa = 10.32 VEE877 pKa = 3.67 PHH879 pKa = 6.6 DD880 pKa = 3.99 PEE882 pKa = 4.81 KK883 pKa = 10.77 AAEE886 pKa = 4.01 ARR888 pKa = 11.84 WRR890 pKa = 11.84 VIWGASMIDD899 pKa = 3.73 VLTQGVTCRR908 pKa = 11.84 MQDD911 pKa = 2.97 KK912 pKa = 10.99 LDD914 pKa = 3.55 ILSYY918 pKa = 10.85 QDD920 pKa = 3.13 GTGKK924 pKa = 10.01 HH925 pKa = 5.08 SQGAGLGHH933 pKa = 6.68 HH934 pKa = 7.26 PEE936 pKa = 5.37 GIQMIGKK943 pKa = 9.25 HH944 pKa = 5.27 IEE946 pKa = 3.76 RR947 pKa = 11.84 LQASGGDD954 pKa = 3.99 LFDD957 pKa = 5.64 ADD959 pKa = 3.65 ASGWDD964 pKa = 3.64 LSVKK968 pKa = 10.04 RR969 pKa = 11.84 DD970 pKa = 4.03 SILMDD975 pKa = 3.29 AEE977 pKa = 3.94 RR978 pKa = 11.84 RR979 pKa = 11.84 IRR981 pKa = 11.84 CYY983 pKa = 9.72 VGPYY987 pKa = 10.07 HH988 pKa = 7.13 EE989 pKa = 4.68 VFKK992 pKa = 10.98 DD993 pKa = 3.6 LQLADD998 pKa = 3.85 AMTNSAHH1005 pKa = 5.71 MLVFGEE1011 pKa = 4.33 YY1012 pKa = 10.5 LFSIYY1017 pKa = 10.37 LAGITASGILPTTAQNSFMRR1037 pKa = 11.84 AFIARR1042 pKa = 11.84 LVGILATFVAGDD1054 pKa = 3.94 DD1055 pKa = 3.64 LMGAGRR1061 pKa = 11.84 FDD1063 pKa = 3.67 PEE1065 pKa = 4.25 YY1066 pKa = 10.18 EE1067 pKa = 4.01 RR1068 pKa = 11.84 KK1069 pKa = 9.63 FGPRR1073 pKa = 11.84 EE1074 pKa = 3.54 KK1075 pKa = 10.29 RR1076 pKa = 11.84 ITYY1079 pKa = 9.48 RR1080 pKa = 11.84 DD1081 pKa = 3.44 PGEE1084 pKa = 4.55 PIEE1087 pKa = 4.17 FTSFLFSKK1095 pKa = 11.01 VGDD1098 pKa = 3.24 TWIADD1103 pKa = 3.48 FTNLGKK1109 pKa = 10.27 SCAKK1113 pKa = 10.27 LAFSPKK1119 pKa = 9.98 SIQPEE1124 pKa = 3.87 QLGGVLYY1131 pKa = 10.55 HH1132 pKa = 7.01 LRR1134 pKa = 11.84 DD1135 pKa = 3.79 NPAKK1139 pKa = 10.26 ADD1141 pKa = 3.82 LFRR1144 pKa = 11.84 EE1145 pKa = 3.59 ICYY1148 pKa = 10.36 RR1149 pKa = 11.84 MEE1151 pKa = 3.89 WPIDD1155 pKa = 3.58 QAVAGPDD1162 pKa = 3.81 GDD1164 pKa = 4.51 CDD1166 pKa = 3.46
Molecular weight: 129.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.267
IPC2_protein 5.283
IPC_protein 5.296
Toseland 5.397
ProMoST 5.474
Dawson 5.372
Bjellqvist 5.461
Wikipedia 5.283
Rodwell 5.308
Grimsley 5.385
Solomon 5.372
Lehninger 5.334
Nozaki 5.537
DTASelect 5.69
Thurlkill 5.474
EMBOSS 5.423
Sillero 5.614
Patrickios 4.037
IPC_peptide 5.385
IPC2_peptide 5.614
IPC2.peptide.svr19 5.578
Protein with the highest isoelectric point:
>tr|A0A1L3KEC8|A0A1L3KEC8_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 11 OX=1922682 PE=4 SV=1
MM1 pKa = 7.7 EE2 pKa = 4.35 NQFTRR7 pKa = 11.84 KK8 pKa = 8.2 QQAALQKK15 pKa = 10.32 AKK17 pKa = 10.15 TPAARR22 pKa = 11.84 EE23 pKa = 4.05 ALKK26 pKa = 10.69 KK27 pKa = 10.74 LYY29 pKa = 8.74 TAQRR33 pKa = 11.84 AGTARR38 pKa = 11.84 PKK40 pKa = 10.56 AQAKK44 pKa = 7.99 PRR46 pKa = 11.84 PRR48 pKa = 11.84 SHH50 pKa = 7.72 AIPNFLDD57 pKa = 3.75 PMCPFPMPTLASEE70 pKa = 4.7 GRR72 pKa = 11.84 ALPHH76 pKa = 6.43 TGLVSSDD83 pKa = 3.37 FTVDD87 pKa = 3.37 TTNTTLLLVANTGHH101 pKa = 6.5 SGTVAFSVKK110 pKa = 9.89 LDD112 pKa = 3.48 ASGGVVAGSIEE123 pKa = 4.86 TYY125 pKa = 9.98 TIPTLSVSDD134 pKa = 4.15 HH135 pKa = 6.51 NGGPTTSRR143 pKa = 11.84 SMKK146 pKa = 10.26 LSVSVVNCTNNYY158 pKa = 8.93 KK159 pKa = 10.5 RR160 pKa = 11.84 GGRR163 pKa = 11.84 VTYY166 pKa = 10.26 INSSQRR172 pKa = 11.84 LPPRR176 pKa = 11.84 NLDD179 pKa = 3.18 LSTEE183 pKa = 4.16 YY184 pKa = 11.2 ASIVSGIKK192 pKa = 9.43 SSPYY196 pKa = 8.67 RR197 pKa = 11.84 RR198 pKa = 11.84 RR199 pKa = 11.84 INGEE203 pKa = 3.87 DD204 pKa = 3.28 LVKK207 pKa = 10.21 PKK209 pKa = 10.56 HH210 pKa = 6.36 LITFPVDD217 pKa = 2.94 NKK219 pKa = 10.29 VYY221 pKa = 10.41 HH222 pKa = 6.64 SYY224 pKa = 11.43 DD225 pKa = 3.16 GFRR228 pKa = 11.84 GTITAGDD235 pKa = 3.99 FLSYY239 pKa = 10.44 MLTPGDD245 pKa = 3.59 NVATKK250 pKa = 10.32 DD251 pKa = 3.61 AEE253 pKa = 4.04 ISPRR257 pKa = 11.84 PMSTVAWIFDD267 pKa = 4.07 PAPDD271 pKa = 3.44 EE272 pKa = 3.95 QAYY275 pKa = 10.52 SFTVRR280 pKa = 11.84 ASFYY284 pKa = 9.93 TRR286 pKa = 11.84 WPLTSVPGQTMHH298 pKa = 6.83 NVPTAPAAVLNAVVDD313 pKa = 3.82 KK314 pKa = 11.57 AEE316 pKa = 3.95 AHH318 pKa = 6.25 GSDD321 pKa = 4.38 LVEE324 pKa = 4.21 LAAGGAAVAMGSRR337 pKa = 11.84 ISGVARR343 pKa = 11.84 SIGSGVQAVGSGIGQALGRR362 pKa = 11.84 AFTSPYY368 pKa = 10.19 GEE370 pKa = 3.96 AVAAGGGEE378 pKa = 4.19 GGLLAGMEE386 pKa = 4.58 GLSSLQALQLPLLAA400 pKa = 5.95
Molecular weight: 42.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.849
IPC2_protein 8.916
IPC_protein 8.887
Toseland 9.428
ProMoST 9.268
Dawson 9.736
Bjellqvist 9.472
Wikipedia 9.94
Rodwell 9.926
Grimsley 9.838
Solomon 9.78
Lehninger 9.736
Nozaki 9.487
DTASelect 9.458
Thurlkill 9.56
EMBOSS 9.867
Sillero 9.663
Patrickios 4.902
IPC_peptide 9.765
IPC2_peptide 8.112
IPC2.peptide.svr19 7.836
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1566
400
1166
783.0
85.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.387 ± 1.143
1.277 ± 0.376
5.875 ± 0.907
6.513 ± 1.578
3.64 ± 0.309
9.004 ± 0.24
2.363 ± 0.175
3.895 ± 0.191
5.3 ± 0.629
8.365 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.554 ± 0.147
2.874 ± 0.424
5.811 ± 0.575
4.215 ± 0.466
5.556 ± 0.027
6.705 ± 1.231
5.492 ± 1.334
6.258 ± 0.601
1.149 ± 0.314
3.768 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here