Pasteurella phage PHB01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Wuhanvirus; Pasteurella virus PHB01

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218M4H9|A0A218M4H9_9CAUD Uncharacterized protein OS=Pasteurella phage PHB01 OX=2006930 PE=4 SV=1
MM1 pKa = 7.85SAPTWFKK8 pKa = 11.05QPFEE12 pKa = 4.38STFDD16 pKa = 3.18EE17 pKa = 5.07EE18 pKa = 4.44YY19 pKa = 9.92KK20 pKa = 10.82HH21 pKa = 6.18RR22 pKa = 11.84VNEE25 pKa = 4.33IILDD29 pKa = 3.99AVRR32 pKa = 11.84HH33 pKa = 5.24TGFRR37 pKa = 11.84LYY39 pKa = 9.41WVEE42 pKa = 3.8VSEE45 pKa = 5.04EE46 pKa = 3.65ISEE49 pKa = 4.44LYY51 pKa = 10.91EE52 pKa = 3.92EE53 pKa = 4.82VNDD56 pKa = 3.77HH57 pKa = 6.29FMNGVTPEE65 pKa = 4.28EE66 pKa = 4.25FNKK69 pKa = 9.54WLYY72 pKa = 9.84PLIVDD77 pKa = 4.97DD78 pKa = 5.84VLFLPFNKK86 pKa = 10.3DD87 pKa = 3.07GEE89 pKa = 4.55FEE91 pKa = 4.04QQ92 pKa = 5.35

Molecular weight: 11.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218M4G1|A0A218M4G1_9CAUD Uncharacterized protein OS=Pasteurella phage PHB01 OX=2006930 PE=4 SV=1
MM1 pKa = 7.57IKK3 pKa = 10.31LKK5 pKa = 10.54RR6 pKa = 11.84HH7 pKa = 4.45ATIGLTDD14 pKa = 4.18TIDD17 pKa = 3.29AYY19 pKa = 10.02EE20 pKa = 4.12RR21 pKa = 11.84KK22 pKa = 9.28IHH24 pKa = 6.85INTLGDD30 pKa = 3.21KK31 pKa = 9.18VTLVFRR37 pKa = 11.84WRR39 pKa = 11.84SRR41 pKa = 11.84SDD43 pKa = 3.23GKK45 pKa = 10.77RR46 pKa = 11.84HH47 pKa = 4.86TQRR50 pKa = 11.84TTLPPSTAILLANQLMVTSTLAGTTTKK77 pKa = 10.98

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11467

38

1361

266.7

29.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.352 ± 0.488

0.89 ± 0.135

6.235 ± 0.21

7.116 ± 0.358

3.793 ± 0.193

7.116 ± 0.347

1.84 ± 0.16

5.799 ± 0.23

7.604 ± 0.374

8.834 ± 0.391

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.159

5.241 ± 0.206

3.331 ± 0.259

4.273 ± 0.176

4.543 ± 0.22

6.549 ± 0.262

6.078 ± 0.209

6.349 ± 0.249

1.221 ± 0.146

3.706 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski