Amino acid dipepetide frequency for Paenibacillus phage Dragolir

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.474AlaAla: 4.474 ± 0.874
0.628AlaCys: 0.628 ± 0.231
3.218AlaAsp: 3.218 ± 0.441
6.358AlaGlu: 6.358 ± 0.933
2.276AlaPhe: 2.276 ± 0.754
4.71AlaGly: 4.71 ± 0.827
1.334AlaHis: 1.334 ± 0.315
3.611AlaIle: 3.611 ± 0.694
5.651AlaLys: 5.651 ± 0.605
6.986AlaLeu: 6.986 ± 1.121
2.355AlaMet: 2.355 ± 0.381
2.669AlaAsn: 2.669 ± 0.64
2.355AlaPro: 2.355 ± 0.435
2.355AlaGln: 2.355 ± 0.558
2.904AlaArg: 2.904 ± 0.386
4.082AlaSer: 4.082 ± 0.784
3.925AlaThr: 3.925 ± 0.547
4.082AlaVal: 4.082 ± 1.127
1.491AlaTrp: 1.491 ± 0.347
1.884AlaTyr: 1.884 ± 0.445
0.0AlaXaa: 0.0 ± 0.0
Cys
0.549CysAla: 0.549 ± 0.167
0.078CysCys: 0.078 ± 0.079
0.235CysAsp: 0.235 ± 0.134
0.785CysGlu: 0.785 ± 0.345
0.392CysPhe: 0.392 ± 0.178
1.099CysGly: 1.099 ± 0.338
0.549CysHis: 0.549 ± 0.197
0.863CysIle: 0.863 ± 0.274
0.785CysLys: 0.785 ± 0.216
0.706CysLeu: 0.706 ± 0.261
0.235CysMet: 0.235 ± 0.146
0.628CysAsn: 0.628 ± 0.247
0.471CysPro: 0.471 ± 0.199
0.392CysGln: 0.392 ± 0.227
0.392CysArg: 0.392 ± 0.156
0.706CysSer: 0.706 ± 0.25
0.157CysThr: 0.157 ± 0.102
0.392CysVal: 0.392 ± 0.163
0.157CysTrp: 0.157 ± 0.111
0.549CysTyr: 0.549 ± 0.265
0.0CysXaa: 0.0 ± 0.0
Asp
3.218AspAla: 3.218 ± 0.505
0.628AspCys: 0.628 ± 0.219
3.14AspAsp: 3.14 ± 0.567
4.631AspGlu: 4.631 ± 0.499
2.119AspPhe: 2.119 ± 0.331
4.16AspGly: 4.16 ± 0.595
0.706AspHis: 0.706 ± 0.28
3.061AspIle: 3.061 ± 0.525
4.317AspLys: 4.317 ± 0.727
4.474AspLeu: 4.474 ± 0.579
1.884AspMet: 1.884 ± 0.375
2.512AspAsn: 2.512 ± 0.409
2.512AspPro: 2.512 ± 0.509
2.119AspGln: 2.119 ± 0.418
3.454AspArg: 3.454 ± 0.545
2.198AspSer: 2.198 ± 0.3
2.747AspThr: 2.747 ± 0.511
3.454AspVal: 3.454 ± 0.467
1.648AspTrp: 1.648 ± 0.434
2.276AspTyr: 2.276 ± 0.456
0.0AspXaa: 0.0 ± 0.0
Glu
5.416GluAla: 5.416 ± 0.654
0.863GluCys: 0.863 ± 0.331
4.082GluAsp: 4.082 ± 0.562
6.358GluGlu: 6.358 ± 1.013
2.983GluPhe: 2.983 ± 0.47
4.003GluGly: 4.003 ± 0.646
1.413GluHis: 1.413 ± 0.43
6.515GluIle: 6.515 ± 0.806
6.044GluLys: 6.044 ± 0.778
9.184GluLeu: 9.184 ± 0.899
2.904GluMet: 2.904 ± 0.535
4.082GluAsn: 4.082 ± 0.578
2.119GluPro: 2.119 ± 0.408
4.553GluGln: 4.553 ± 0.63
4.396GluArg: 4.396 ± 0.51
4.082GluSer: 4.082 ± 0.574
4.003GluThr: 4.003 ± 0.489
5.102GluVal: 5.102 ± 0.671
1.334GluTrp: 1.334 ± 0.488
2.826GluTyr: 2.826 ± 0.611
0.0GluXaa: 0.0 ± 0.0
Phe
2.276PheAla: 2.276 ± 0.698
0.549PheCys: 0.549 ± 0.201
2.433PheAsp: 2.433 ± 0.41
3.375PheGlu: 3.375 ± 0.637
1.334PhePhe: 1.334 ± 0.34
3.14PheGly: 3.14 ± 0.409
0.471PheHis: 0.471 ± 0.239
2.512PheIle: 2.512 ± 0.454
2.433PheLys: 2.433 ± 0.38
2.904PheLeu: 2.904 ± 0.543
0.942PheMet: 0.942 ± 0.298
1.962PheAsn: 1.962 ± 0.447
1.099PhePro: 1.099 ± 0.333
1.727PheGln: 1.727 ± 0.53
0.942PheArg: 0.942 ± 0.265
2.512PheSer: 2.512 ± 0.482
1.884PheThr: 1.884 ± 0.466
2.119PheVal: 2.119 ± 0.387
0.471PheTrp: 0.471 ± 0.207
1.648PheTyr: 1.648 ± 0.415
0.0PheXaa: 0.0 ± 0.0
Gly
3.768GlyAla: 3.768 ± 0.828
0.549GlyCys: 0.549 ± 0.204
4.945GlyAsp: 4.945 ± 0.506
4.945GlyGlu: 4.945 ± 0.728
2.119GlyPhe: 2.119 ± 0.248
5.259GlyGly: 5.259 ± 0.649
1.02GlyHis: 1.02 ± 0.303
4.396GlyIle: 4.396 ± 0.768
6.122GlyLys: 6.122 ± 0.869
5.416GlyLeu: 5.416 ± 0.753
1.727GlyMet: 1.727 ± 0.373
2.904GlyAsn: 2.904 ± 0.618
0.863GlyPro: 0.863 ± 0.223
2.826GlyGln: 2.826 ± 0.583
2.59GlyArg: 2.59 ± 0.565
3.532GlySer: 3.532 ± 0.497
3.925GlyThr: 3.925 ± 0.581
5.416GlyVal: 5.416 ± 0.675
1.334GlyTrp: 1.334 ± 0.382
2.433GlyTyr: 2.433 ± 0.457
0.0GlyXaa: 0.0 ± 0.0
His
1.334HisAla: 1.334 ± 0.324
0.235HisCys: 0.235 ± 0.129
1.02HisAsp: 1.02 ± 0.295
1.256HisGlu: 1.256 ± 0.327
0.549HisPhe: 0.549 ± 0.186
0.785HisGly: 0.785 ± 0.244
0.785HisHis: 0.785 ± 0.237
1.727HisIle: 1.727 ± 0.421
1.02HisLys: 1.02 ± 0.313
1.884HisLeu: 1.884 ± 0.378
0.314HisMet: 0.314 ± 0.149
0.628HisAsn: 0.628 ± 0.224
0.471HisPro: 0.471 ± 0.207
1.02HisGln: 1.02 ± 0.299
0.628HisArg: 0.628 ± 0.19
1.256HisSer: 1.256 ± 0.289
0.785HisThr: 0.785 ± 0.239
1.413HisVal: 1.413 ± 0.337
0.157HisTrp: 0.157 ± 0.11
0.471HisTyr: 0.471 ± 0.187
0.0HisXaa: 0.0 ± 0.0
Ile
4.945IleAla: 4.945 ± 1.161
0.863IleCys: 0.863 ± 0.257
4.082IleAsp: 4.082 ± 0.686
6.279IleGlu: 6.279 ± 0.692
1.413IlePhe: 1.413 ± 0.464
5.102IleGly: 5.102 ± 0.799
0.706IleHis: 0.706 ± 0.258
3.768IleIle: 3.768 ± 0.816
5.495IleLys: 5.495 ± 0.76
4.631IleLeu: 4.631 ± 0.696
0.863IleMet: 0.863 ± 0.415
2.59IleAsn: 2.59 ± 0.415
2.983IlePro: 2.983 ± 0.543
3.611IleGln: 3.611 ± 0.534
3.297IleArg: 3.297 ± 0.418
3.061IleSer: 3.061 ± 0.397
3.061IleThr: 3.061 ± 0.415
4.082IleVal: 4.082 ± 0.647
0.628IleTrp: 0.628 ± 0.208
1.884IleTyr: 1.884 ± 0.44
0.0IleXaa: 0.0 ± 0.0
Lys
5.73LysAla: 5.73 ± 0.745
0.628LysCys: 0.628 ± 0.241
2.904LysAsp: 2.904 ± 0.493
7.064LysGlu: 7.064 ± 0.697
2.826LysPhe: 2.826 ± 0.511
5.495LysGly: 5.495 ± 0.561
1.256LysHis: 1.256 ± 0.307
3.454LysIle: 3.454 ± 0.555
6.044LysLys: 6.044 ± 0.861
7.143LysLeu: 7.143 ± 0.748
2.59LysMet: 2.59 ± 0.42
3.611LysAsn: 3.611 ± 0.445
2.747LysPro: 2.747 ± 0.595
4.317LysGln: 4.317 ± 0.557
5.102LysArg: 5.102 ± 0.609
4.082LysSer: 4.082 ± 0.487
4.553LysThr: 4.553 ± 0.593
5.102LysVal: 5.102 ± 0.578
1.491LysTrp: 1.491 ± 0.321
2.59LysTyr: 2.59 ± 0.499
0.0LysXaa: 0.0 ± 0.0
Leu
6.044LeuAla: 6.044 ± 0.784
0.863LeuCys: 0.863 ± 0.325
5.181LeuAsp: 5.181 ± 0.575
8.006LeuGlu: 8.006 ± 0.877
3.689LeuPhe: 3.689 ± 0.435
6.122LeuGly: 6.122 ± 1.012
1.491LeuHis: 1.491 ± 0.36
3.846LeuIle: 3.846 ± 0.389
6.593LeuLys: 6.593 ± 0.734
7.378LeuLeu: 7.378 ± 0.875
2.119LeuMet: 2.119 ± 0.435
4.239LeuAsn: 4.239 ± 0.522
3.689LeuPro: 3.689 ± 0.542
3.689LeuGln: 3.689 ± 0.566
4.867LeuArg: 4.867 ± 0.662
6.829LeuSer: 6.829 ± 0.657
5.338LeuThr: 5.338 ± 0.512
4.396LeuVal: 4.396 ± 0.462
1.256LeuTrp: 1.256 ± 0.523
2.512LeuTyr: 2.512 ± 0.421
0.0LeuXaa: 0.0 ± 0.0
Met
1.884MetAla: 1.884 ± 0.432
0.157MetCys: 0.157 ± 0.125
1.02MetAsp: 1.02 ± 0.286
2.198MetGlu: 2.198 ± 0.319
0.942MetPhe: 0.942 ± 0.278
1.334MetGly: 1.334 ± 0.279
0.628MetHis: 0.628 ± 0.209
1.491MetIle: 1.491 ± 0.329
2.198MetLys: 2.198 ± 0.463
2.669MetLeu: 2.669 ± 0.409
0.628MetMet: 0.628 ± 0.246
1.727MetAsn: 1.727 ± 0.396
1.099MetPro: 1.099 ± 0.372
1.177MetGln: 1.177 ± 0.333
1.491MetArg: 1.491 ± 0.339
1.962MetSer: 1.962 ± 0.394
1.884MetThr: 1.884 ± 0.446
1.491MetVal: 1.491 ± 0.315
0.235MetTrp: 0.235 ± 0.12
0.785MetTyr: 0.785 ± 0.304
0.0MetXaa: 0.0 ± 0.0
Asn
3.14AsnAla: 3.14 ± 0.781
0.549AsnCys: 0.549 ± 0.215
2.198AsnAsp: 2.198 ± 0.386
3.061AsnGlu: 3.061 ± 0.526
1.177AsnPhe: 1.177 ± 0.312
3.768AsnGly: 3.768 ± 0.527
0.549AsnHis: 0.549 ± 0.182
2.433AsnIle: 2.433 ± 0.468
3.454AsnLys: 3.454 ± 0.507
4.082AsnLeu: 4.082 ± 0.448
1.491AsnMet: 1.491 ± 0.334
1.962AsnAsn: 1.962 ± 0.357
1.962AsnPro: 1.962 ± 0.477
2.826AsnGln: 2.826 ± 0.459
2.904AsnArg: 2.904 ± 0.616
2.512AsnSer: 2.512 ± 0.492
2.59AsnThr: 2.59 ± 0.432
2.826AsnVal: 2.826 ± 0.448
0.471AsnTrp: 0.471 ± 0.229
1.57AsnTyr: 1.57 ± 0.358
0.0AsnXaa: 0.0 ± 0.0
Pro
2.355ProAla: 2.355 ± 0.432
0.235ProCys: 0.235 ± 0.128
2.041ProAsp: 2.041 ± 0.413
2.747ProGlu: 2.747 ± 0.402
1.02ProPhe: 1.02 ± 0.281
2.119ProGly: 2.119 ± 0.443
0.863ProHis: 0.863 ± 0.251
2.826ProIle: 2.826 ± 0.517
3.14ProLys: 3.14 ± 0.513
2.669ProLeu: 2.669 ± 0.425
0.628ProMet: 0.628 ± 0.288
1.884ProAsn: 1.884 ± 0.322
1.256ProPro: 1.256 ± 0.304
0.942ProGln: 0.942 ± 0.307
0.863ProArg: 0.863 ± 0.258
2.747ProSer: 2.747 ± 0.562
2.119ProThr: 2.119 ± 0.467
2.904ProVal: 2.904 ± 0.673
0.157ProTrp: 0.157 ± 0.104
1.334ProTyr: 1.334 ± 0.284
0.0ProXaa: 0.0 ± 0.0
Gln
3.925GlnAla: 3.925 ± 0.613
0.471GlnCys: 0.471 ± 0.23
2.198GlnAsp: 2.198 ± 0.44
3.611GlnGlu: 3.611 ± 0.49
2.276GlnPhe: 2.276 ± 0.407
2.276GlnGly: 2.276 ± 0.385
1.099GlnHis: 1.099 ± 0.305
2.826GlnIle: 2.826 ± 0.47
3.14GlnLys: 3.14 ± 0.529
3.846GlnLeu: 3.846 ± 0.643
2.198GlnMet: 2.198 ± 0.408
1.727GlnAsn: 1.727 ± 0.382
1.334GlnPro: 1.334 ± 0.31
2.119GlnGln: 2.119 ± 0.545
2.512GlnArg: 2.512 ± 0.456
2.119GlnSer: 2.119 ± 0.443
2.355GlnThr: 2.355 ± 0.428
2.983GlnVal: 2.983 ± 0.411
0.785GlnTrp: 0.785 ± 0.241
1.256GlnTyr: 1.256 ± 0.336
0.0GlnXaa: 0.0 ± 0.0
Arg
2.904ArgAla: 2.904 ± 0.334
0.392ArgCys: 0.392 ± 0.161
2.747ArgAsp: 2.747 ± 0.579
4.16ArgGlu: 4.16 ± 0.692
1.962ArgPhe: 1.962 ± 0.382
2.669ArgGly: 2.669 ± 0.492
1.334ArgHis: 1.334 ± 0.344
3.454ArgIle: 3.454 ± 0.574
4.317ArgLys: 4.317 ± 0.489
5.024ArgLeu: 5.024 ± 0.709
1.57ArgMet: 1.57 ± 0.285
2.198ArgAsn: 2.198 ± 0.305
1.57ArgPro: 1.57 ± 0.36
1.648ArgGln: 1.648 ± 0.412
3.14ArgArg: 3.14 ± 0.637
2.433ArgSer: 2.433 ± 0.437
2.433ArgThr: 2.433 ± 0.396
4.239ArgVal: 4.239 ± 0.597
1.02ArgTrp: 1.02 ± 0.271
2.041ArgTyr: 2.041 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
4.71SerAla: 4.71 ± 0.695
0.549SerCys: 0.549 ± 0.19
2.355SerAsp: 2.355 ± 0.335
4.16SerGlu: 4.16 ± 0.682
2.59SerPhe: 2.59 ± 0.498
3.532SerGly: 3.532 ± 0.403
0.863SerHis: 0.863 ± 0.284
5.416SerIle: 5.416 ± 0.678
4.396SerLys: 4.396 ± 0.637
4.474SerLeu: 4.474 ± 0.563
1.648SerMet: 1.648 ± 0.353
2.355SerAsn: 2.355 ± 0.484
1.648SerPro: 1.648 ± 0.357
2.198SerGln: 2.198 ± 0.45
3.375SerArg: 3.375 ± 0.635
3.061SerSer: 3.061 ± 0.529
3.375SerThr: 3.375 ± 0.505
3.689SerVal: 3.689 ± 0.525
0.785SerTrp: 0.785 ± 0.243
2.119SerTyr: 2.119 ± 0.401
0.0SerXaa: 0.0 ± 0.0
Thr
3.925ThrAla: 3.925 ± 0.513
0.471ThrCys: 0.471 ± 0.233
2.433ThrAsp: 2.433 ± 0.535
3.925ThrGlu: 3.925 ± 0.539
2.276ThrPhe: 2.276 ± 0.38
3.768ThrGly: 3.768 ± 0.378
0.863ThrHis: 0.863 ± 0.242
3.297ThrIle: 3.297 ± 0.502
4.16ThrLys: 4.16 ± 0.486
4.474ThrLeu: 4.474 ± 0.72
0.628ThrMet: 0.628 ± 0.221
2.355ThrAsn: 2.355 ± 0.544
2.669ThrPro: 2.669 ± 0.628
2.198ThrGln: 2.198 ± 0.464
2.512ThrArg: 2.512 ± 0.432
3.061ThrSer: 3.061 ± 0.48
3.925ThrThr: 3.925 ± 0.692
4.788ThrVal: 4.788 ± 0.425
0.863ThrTrp: 0.863 ± 0.291
1.962ThrTyr: 1.962 ± 0.436
0.0ThrXaa: 0.0 ± 0.0
Val
4.317ValAla: 4.317 ± 0.537
0.549ValCys: 0.549 ± 0.219
5.024ValAsp: 5.024 ± 0.583
5.338ValGlu: 5.338 ± 0.58
2.983ValPhe: 2.983 ± 0.601
3.846ValGly: 3.846 ± 0.435
0.785ValHis: 0.785 ± 0.277
5.573ValIle: 5.573 ± 1.212
5.573ValLys: 5.573 ± 0.617
6.201ValLeu: 6.201 ± 0.638
1.099ValMet: 1.099 ± 0.435
2.904ValAsn: 2.904 ± 0.505
2.512ValPro: 2.512 ± 0.385
2.669ValGln: 2.669 ± 0.476
3.218ValArg: 3.218 ± 0.49
4.16ValSer: 4.16 ± 0.628
3.061ValThr: 3.061 ± 0.558
6.044ValVal: 6.044 ± 0.648
0.942ValTrp: 0.942 ± 0.308
1.884ValTyr: 1.884 ± 0.459
0.0ValXaa: 0.0 ± 0.0
Trp
0.628TrpAla: 0.628 ± 0.227
0.157TrpCys: 0.157 ± 0.107
1.413TrpAsp: 1.413 ± 0.324
1.334TrpGlu: 1.334 ± 0.28
0.549TrpPhe: 0.549 ± 0.19
0.863TrpGly: 0.863 ± 0.306
0.392TrpHis: 0.392 ± 0.147
0.863TrpIle: 0.863 ± 0.341
1.334TrpLys: 1.334 ± 0.269
1.334TrpLeu: 1.334 ± 0.302
0.392TrpMet: 0.392 ± 0.178
0.863TrpAsn: 0.863 ± 0.31
0.235TrpPro: 0.235 ± 0.121
0.706TrpGln: 0.706 ± 0.272
0.863TrpArg: 0.863 ± 0.273
1.413TrpSer: 1.413 ± 0.358
0.392TrpThr: 0.392 ± 0.208
1.099TrpVal: 1.099 ± 0.328
0.235TrpTrp: 0.235 ± 0.143
0.863TrpTyr: 0.863 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.805TyrAla: 1.805 ± 0.426
0.706TyrCys: 0.706 ± 0.235
2.59TyrAsp: 2.59 ± 0.509
2.669TyrGlu: 2.669 ± 0.48
1.334TyrPhe: 1.334 ± 0.323
2.041TyrGly: 2.041 ± 0.494
0.628TyrHis: 0.628 ± 0.213
1.413TyrIle: 1.413 ± 0.313
2.512TyrLys: 2.512 ± 0.375
2.669TyrLeu: 2.669 ± 0.499
0.706TyrMet: 0.706 ± 0.227
1.727TyrAsn: 1.727 ± 0.289
1.256TyrPro: 1.256 ± 0.33
1.962TyrGln: 1.962 ± 0.43
1.805TyrArg: 1.805 ± 0.356
1.491TyrSer: 1.491 ± 0.296
2.041TyrThr: 2.041 ± 0.505
3.061TyrVal: 3.061 ± 0.557
0.471TyrTrp: 0.471 ± 0.18
1.177TyrTyr: 1.177 ± 0.312
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (12741 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski