Amino acid dipepetide frequency for Ruminococcus sp. AM36-2AA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.314AlaAla: 7.314 ± 0.112
1.131AlaCys: 1.131 ± 0.033
4.704AlaAsp: 4.704 ± 0.077
5.537AlaGlu: 5.537 ± 0.1
3.045AlaPhe: 3.045 ± 0.058
6.254AlaGly: 6.254 ± 0.101
1.081AlaHis: 1.081 ± 0.03
4.69AlaIle: 4.69 ± 0.07
4.697AlaLys: 4.697 ± 0.074
6.505AlaLeu: 6.505 ± 0.091
2.41AlaMet: 2.41 ± 0.055
2.401AlaAsn: 2.401 ± 0.048
1.98AlaPro: 1.98 ± 0.046
2.325AlaGln: 2.325 ± 0.053
2.883AlaArg: 2.883 ± 0.054
3.915AlaSer: 3.915 ± 0.058
3.513AlaThr: 3.513 ± 0.09
6.208AlaVal: 6.208 ± 0.087
0.603AlaTrp: 0.603 ± 0.026
2.682AlaTyr: 2.682 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.056CysAla: 1.056 ± 0.034
0.31CysCys: 0.31 ± 0.018
0.836CysAsp: 0.836 ± 0.031
0.975CysGlu: 0.975 ± 0.042
0.619CysPhe: 0.619 ± 0.023
1.624CysGly: 1.624 ± 0.05
0.341CysHis: 0.341 ± 0.018
1.116CysIle: 1.116 ± 0.035
0.826CysLys: 0.826 ± 0.028
1.174CysLeu: 1.174 ± 0.036
0.48CysMet: 0.48 ± 0.022
0.579CysAsn: 0.579 ± 0.027
0.625CysPro: 0.625 ± 0.027
0.465CysGln: 0.465 ± 0.025
0.742CysArg: 0.742 ± 0.025
0.932CysSer: 0.932 ± 0.039
0.929CysThr: 0.929 ± 0.034
1.048CysVal: 1.048 ± 0.031
0.145CysTrp: 0.145 ± 0.011
0.532CysTyr: 0.532 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
4.098AspAla: 4.098 ± 0.078
0.799AspCys: 0.799 ± 0.032
3.008AspAsp: 3.008 ± 0.066
4.581AspGlu: 4.581 ± 0.08
2.625AspPhe: 2.625 ± 0.049
4.693AspGly: 4.693 ± 0.089
1.019AspHis: 1.019 ± 0.033
4.451AspIle: 4.451 ± 0.069
3.611AspLys: 3.611 ± 0.068
4.69AspLeu: 4.69 ± 0.066
1.902AspMet: 1.902 ± 0.04
2.338AspAsn: 2.338 ± 0.064
2.009AspPro: 2.009 ± 0.043
1.675AspGln: 1.675 ± 0.047
2.395AspArg: 2.395 ± 0.053
3.483AspSer: 3.483 ± 0.069
3.342AspThr: 3.342 ± 0.068
3.846AspVal: 3.846 ± 0.071
0.609AspTrp: 0.609 ± 0.026
2.682AspTyr: 2.682 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
5.527GluAla: 5.527 ± 0.085
0.827GluCys: 0.827 ± 0.028
4.694GluAsp: 4.694 ± 0.082
7.42GluGlu: 7.42 ± 0.13
2.705GluPhe: 2.705 ± 0.051
4.342GluGly: 4.342 ± 0.082
1.362GluHis: 1.362 ± 0.038
5.809GluIle: 5.809 ± 0.083
7.505GluLys: 7.505 ± 0.104
6.585GluLeu: 6.585 ± 0.082
2.415GluMet: 2.415 ± 0.053
4.413GluAsn: 4.413 ± 0.065
1.812GluPro: 1.812 ± 0.046
2.827GluGln: 2.827 ± 0.056
3.115GluArg: 3.115 ± 0.061
3.428GluSer: 3.428 ± 0.064
3.972GluThr: 3.972 ± 0.062
4.395GluVal: 4.395 ± 0.066
0.617GluTrp: 0.617 ± 0.024
3.049GluTyr: 3.049 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
2.791PheAla: 2.791 ± 0.051
0.842PheCys: 0.842 ± 0.028
2.372PheAsp: 2.372 ± 0.048
2.455PheGlu: 2.455 ± 0.048
1.832PhePhe: 1.832 ± 0.05
3.089PheGly: 3.089 ± 0.061
0.924PheHis: 0.924 ± 0.031
2.486PheIle: 2.486 ± 0.057
1.847PheLys: 1.847 ± 0.043
3.91PheLeu: 3.91 ± 0.081
1.215PheMet: 1.215 ± 0.036
1.483PheAsn: 1.483 ± 0.042
1.506PhePro: 1.506 ± 0.042
1.394PheGln: 1.394 ± 0.034
1.743PheArg: 1.743 ± 0.047
2.951PheSer: 2.951 ± 0.062
2.355PheThr: 2.355 ± 0.043
2.795PheVal: 2.795 ± 0.057
0.418PheTrp: 0.418 ± 0.023
1.75PheTyr: 1.75 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
5.01GlyAla: 5.01 ± 0.091
1.349GlyCys: 1.349 ± 0.047
3.752GlyAsp: 3.752 ± 0.072
4.8GlyGlu: 4.8 ± 0.067
3.116GlyPhe: 3.116 ± 0.065
4.818GlyGly: 4.818 ± 0.089
1.328GlyHis: 1.328 ± 0.041
6.315GlyIle: 6.315 ± 0.094
5.988GlyLys: 5.988 ± 0.08
5.648GlyLeu: 5.648 ± 0.099
2.514GlyMet: 2.514 ± 0.057
3.347GlyAsn: 3.347 ± 0.064
1.3GlyPro: 1.3 ± 0.046
2.108GlyGln: 2.108 ± 0.044
3.032GlyArg: 3.032 ± 0.062
4.197GlySer: 4.197 ± 0.073
4.49GlyThr: 4.49 ± 0.079
4.966GlyVal: 4.966 ± 0.076
0.739GlyTrp: 0.739 ± 0.028
3.326GlyTyr: 3.326 ± 0.062
0.0GlyXaa: 0.0 ± 0.0
His
1.082HisAla: 1.082 ± 0.034
0.337HisCys: 0.337 ± 0.026
0.964HisAsp: 0.964 ± 0.039
1.163HisGlu: 1.163 ± 0.036
0.81HisPhe: 0.81 ± 0.035
1.338HisGly: 1.338 ± 0.041
0.458HisHis: 0.458 ± 0.036
1.343HisIle: 1.343 ± 0.033
1.071HisLys: 1.071 ± 0.032
1.458HisLeu: 1.458 ± 0.041
0.604HisMet: 0.604 ± 0.024
0.713HisAsn: 0.713 ± 0.024
0.88HisPro: 0.88 ± 0.031
0.532HisGln: 0.532 ± 0.02
0.828HisArg: 0.828 ± 0.033
0.976HisSer: 0.976 ± 0.035
0.963HisThr: 0.963 ± 0.036
1.245HisVal: 1.245 ± 0.037
0.162HisTrp: 0.162 ± 0.013
0.771HisTyr: 0.771 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.285IleAla: 5.285 ± 0.087
1.366IleCys: 1.366 ± 0.039
3.769IleAsp: 3.769 ± 0.063
4.329IleGlu: 4.329 ± 0.059
2.956IlePhe: 2.956 ± 0.066
4.881IleGly: 4.881 ± 0.083
1.477IleHis: 1.477 ± 0.041
4.56IleIle: 4.56 ± 0.083
3.743IleLys: 3.743 ± 0.064
7.186IleLeu: 7.186 ± 0.104
2.018IleMet: 2.018 ± 0.049
2.763IleAsn: 2.763 ± 0.06
3.201IlePro: 3.201 ± 0.06
2.452IleGln: 2.452 ± 0.058
3.957IleArg: 3.957 ± 0.062
4.859IleSer: 4.859 ± 0.076
4.349IleThr: 4.349 ± 0.083
4.715IleVal: 4.715 ± 0.082
0.638IleTrp: 0.638 ± 0.028
2.748IleTyr: 2.748 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
5.175LysAla: 5.175 ± 0.089
0.761LysCys: 0.761 ± 0.026
4.286LysAsp: 4.286 ± 0.063
6.788LysGlu: 6.788 ± 0.107
2.11LysPhe: 2.11 ± 0.05
4.237LysGly: 4.237 ± 0.07
0.994LysHis: 0.994 ± 0.035
5.188LysIle: 5.188 ± 0.078
6.886LysLys: 6.886 ± 0.101
5.425LysLeu: 5.425 ± 0.073
2.344LysMet: 2.344 ± 0.044
3.804LysAsn: 3.804 ± 0.06
2.02LysPro: 2.02 ± 0.047
2.217LysGln: 2.217 ± 0.048
3.067LysArg: 3.067 ± 0.06
3.405LysSer: 3.405 ± 0.055
4.115LysThr: 4.115 ± 0.078
4.399LysVal: 4.399 ± 0.072
0.616LysTrp: 0.616 ± 0.022
3.015LysTyr: 3.015 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
6.501LeuAla: 6.501 ± 0.098
1.468LeuCys: 1.468 ± 0.037
5.076LeuAsp: 5.076 ± 0.076
6.302LeuGlu: 6.302 ± 0.088
3.496LeuPhe: 3.496 ± 0.071
5.985LeuGly: 5.985 ± 0.086
1.58LeuHis: 1.58 ± 0.042
5.768LeuIle: 5.768 ± 0.104
6.398LeuLys: 6.398 ± 0.083
8.066LeuLeu: 8.066 ± 0.14
2.53LeuMet: 2.53 ± 0.057
3.712LeuAsn: 3.712 ± 0.06
3.462LeuPro: 3.462 ± 0.063
2.688LeuGln: 2.688 ± 0.063
3.655LeuArg: 3.655 ± 0.07
5.952LeuSer: 5.952 ± 0.083
5.147LeuThr: 5.147 ± 0.067
5.358LeuVal: 5.358 ± 0.091
0.723LeuTrp: 0.723 ± 0.028
3.243LeuTyr: 3.243 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.502MetAla: 2.502 ± 0.057
0.388MetCys: 0.388 ± 0.022
1.974MetAsp: 1.974 ± 0.044
2.752MetGlu: 2.752 ± 0.06
1.02MetPhe: 1.02 ± 0.036
2.182MetGly: 2.182 ± 0.054
0.435MetHis: 0.435 ± 0.024
2.228MetIle: 2.228 ± 0.052
2.657MetLys: 2.657 ± 0.054
2.679MetLeu: 2.679 ± 0.052
0.989MetMet: 0.989 ± 0.034
1.543MetAsn: 1.543 ± 0.039
1.182MetPro: 1.182 ± 0.035
1.054MetGln: 1.054 ± 0.033
1.252MetArg: 1.252 ± 0.035
1.751MetSer: 1.751 ± 0.041
1.721MetThr: 1.721 ± 0.039
1.915MetVal: 1.915 ± 0.045
0.205MetTrp: 0.205 ± 0.014
0.893MetTyr: 0.893 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.096AsnAla: 3.096 ± 0.075
0.688AsnCys: 0.688 ± 0.029
2.136AsnAsp: 2.136 ± 0.051
2.702AsnGlu: 2.702 ± 0.057
1.585AsnPhe: 1.585 ± 0.041
3.694AsnGly: 3.694 ± 0.068
0.827AsnHis: 0.827 ± 0.031
3.426AsnIle: 3.426 ± 0.06
2.553AsnLys: 2.553 ± 0.054
3.699AsnLeu: 3.699 ± 0.058
1.395AsnMet: 1.395 ± 0.033
1.779AsnAsn: 1.779 ± 0.053
1.923AsnPro: 1.923 ± 0.045
1.431AsnGln: 1.431 ± 0.04
2.066AsnArg: 2.066 ± 0.049
2.43AsnSer: 2.43 ± 0.056
2.504AsnThr: 2.504 ± 0.056
2.746AsnVal: 2.746 ± 0.059
0.434AsnTrp: 0.434 ± 0.021
1.821AsnTyr: 1.821 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
2.516ProAla: 2.516 ± 0.054
0.439ProCys: 0.439 ± 0.022
2.463ProAsp: 2.463 ± 0.055
3.703ProGlu: 3.703 ± 0.063
1.501ProPhe: 1.501 ± 0.041
2.513ProGly: 2.513 ± 0.053
0.563ProHis: 0.563 ± 0.025
1.842ProIle: 1.842 ± 0.046
1.942ProLys: 1.942 ± 0.044
2.761ProLeu: 2.761 ± 0.052
0.881ProMet: 0.881 ± 0.026
1.092ProAsn: 1.092 ± 0.032
0.68ProPro: 0.68 ± 0.03
1.051ProGln: 1.051 ± 0.029
0.999ProArg: 0.999 ± 0.03
1.799ProSer: 1.799 ± 0.039
1.648ProThr: 1.648 ± 0.046
3.165ProVal: 3.165 ± 0.059
0.356ProTrp: 0.356 ± 0.019
1.4ProTyr: 1.4 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
2.494GlnAla: 2.494 ± 0.052
0.346GlnCys: 0.346 ± 0.016
1.746GlnAsp: 1.746 ± 0.044
2.872GlnGlu: 2.872 ± 0.058
1.073GlnPhe: 1.073 ± 0.029
2.046GlnGly: 2.046 ± 0.044
0.445GlnHis: 0.445 ± 0.023
2.53GlnIle: 2.53 ± 0.048
2.936GlnLys: 2.936 ± 0.059
2.708GlnLeu: 2.708 ± 0.053
1.155GlnMet: 1.155 ± 0.034
1.65GlnAsn: 1.65 ± 0.04
0.944GlnPro: 0.944 ± 0.038
1.247GlnGln: 1.247 ± 0.042
1.34GlnArg: 1.34 ± 0.044
1.555GlnSer: 1.555 ± 0.047
1.643GlnThr: 1.643 ± 0.044
2.063GlnVal: 2.063 ± 0.041
0.335GlnTrp: 0.335 ± 0.021
1.389GlnTyr: 1.389 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.625ArgAla: 2.625 ± 0.053
0.624ArgCys: 0.624 ± 0.023
2.268ArgAsp: 2.268 ± 0.047
3.916ArgGlu: 3.916 ± 0.079
1.768ArgPhe: 1.768 ± 0.041
2.462ArgGly: 2.462 ± 0.053
0.802ArgHis: 0.802 ± 0.029
3.371ArgIle: 3.371 ± 0.064
3.875ArgLys: 3.875 ± 0.068
3.676ArgLeu: 3.676 ± 0.072
1.528ArgMet: 1.528 ± 0.049
2.198ArgAsn: 2.198 ± 0.048
1.322ArgPro: 1.322 ± 0.037
1.657ArgGln: 1.657 ± 0.044
2.273ArgArg: 2.273 ± 0.056
2.195ArgSer: 2.195 ± 0.042
2.305ArgThr: 2.305 ± 0.048
2.563ArgVal: 2.563 ± 0.055
0.385ArgTrp: 0.385 ± 0.021
1.934ArgTyr: 1.934 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
4.321SerAla: 4.321 ± 0.075
0.872SerCys: 0.872 ± 0.029
3.478SerAsp: 3.478 ± 0.081
3.885SerGlu: 3.885 ± 0.059
2.522SerPhe: 2.522 ± 0.051
5.093SerGly: 5.093 ± 0.085
1.07SerHis: 1.07 ± 0.034
3.9SerIle: 3.9 ± 0.066
3.168SerLys: 3.168 ± 0.063
5.019SerLeu: 5.019 ± 0.074
1.909SerMet: 1.909 ± 0.042
2.145SerAsn: 2.145 ± 0.051
1.776SerPro: 1.776 ± 0.041
1.956SerGln: 1.956 ± 0.045
2.911SerArg: 2.911 ± 0.052
3.88SerSer: 3.88 ± 0.095
3.011SerThr: 3.011 ± 0.069
4.425SerVal: 4.425 ± 0.067
0.575SerTrp: 0.575 ± 0.026
2.419SerTyr: 2.419 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
4.624ThrAla: 4.624 ± 0.081
0.783ThrCys: 0.783 ± 0.056
3.564ThrAsp: 3.564 ± 0.083
4.366ThrGlu: 4.366 ± 0.089
2.242ThrPhe: 2.242 ± 0.042
5.107ThrGly: 5.107 ± 0.084
0.828ThrHis: 0.828 ± 0.032
3.807ThrIle: 3.807 ± 0.067
3.129ThrLys: 3.129 ± 0.069
4.862ThrLeu: 4.862 ± 0.083
1.474ThrMet: 1.474 ± 0.041
1.915ThrAsn: 1.915 ± 0.045
2.369ThrPro: 2.369 ± 0.053
1.449ThrGln: 1.449 ± 0.04
2.102ThrArg: 2.102 ± 0.044
3.153ThrSer: 3.153 ± 0.065
3.038ThrThr: 3.038 ± 0.078
4.815ThrVal: 4.815 ± 0.109
0.532ThrTrp: 0.532 ± 0.021
2.234ThrTyr: 2.234 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
4.709ValAla: 4.709 ± 0.076
1.223ValCys: 1.223 ± 0.036
3.709ValAsp: 3.709 ± 0.062
4.666ValGlu: 4.666 ± 0.069
2.992ValPhe: 2.992 ± 0.061
4.1ValGly: 4.1 ± 0.079
1.099ValHis: 1.099 ± 0.031
5.088ValIle: 5.088 ± 0.078
4.835ValLys: 4.835 ± 0.072
6.553ValLeu: 6.553 ± 0.094
2.058ValMet: 2.058 ± 0.045
2.806ValAsn: 2.806 ± 0.052
2.626ValPro: 2.626 ± 0.05
2.121ValGln: 2.121 ± 0.048
2.941ValArg: 2.941 ± 0.055
4.615ValSer: 4.615 ± 0.077
4.453ValThr: 4.453 ± 0.087
4.644ValVal: 4.644 ± 0.081
0.589ValTrp: 0.589 ± 0.024
2.583ValTyr: 2.583 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.509TrpAla: 0.509 ± 0.025
0.147TrpCys: 0.147 ± 0.011
0.569TrpAsp: 0.569 ± 0.024
0.672TrpGlu: 0.672 ± 0.026
0.392TrpPhe: 0.392 ± 0.02
0.639TrpGly: 0.639 ± 0.027
0.173TrpHis: 0.173 ± 0.014
0.656TrpIle: 0.656 ± 0.024
0.813TrpLys: 0.813 ± 0.031
0.865TrpLeu: 0.865 ± 0.03
0.362TrpMet: 0.362 ± 0.02
0.528TrpAsn: 0.528 ± 0.024
0.211TrpPro: 0.211 ± 0.014
0.314TrpGln: 0.314 ± 0.017
0.362TrpArg: 0.362 ± 0.02
0.491TrpSer: 0.491 ± 0.023
0.467TrpThr: 0.467 ± 0.024
0.48TrpVal: 0.48 ± 0.021
0.107TrpTrp: 0.107 ± 0.009
0.385TrpTyr: 0.385 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.644TyrAla: 2.644 ± 0.051
0.653TyrCys: 0.653 ± 0.027
2.487TyrAsp: 2.487 ± 0.059
2.936TyrGlu: 2.936 ± 0.055
1.749TyrPhe: 1.749 ± 0.042
3.045TyrGly: 3.045 ± 0.057
0.893TyrHis: 0.893 ± 0.03
2.678TyrIle: 2.678 ± 0.054
2.371TyrLys: 2.371 ± 0.053
3.64TyrLeu: 3.64 ± 0.062
1.138TyrMet: 1.138 ± 0.032
1.724TyrAsn: 1.724 ± 0.045
1.488TyrPro: 1.488 ± 0.043
1.557TyrGln: 1.557 ± 0.042
2.085TyrArg: 2.085 ± 0.05
2.366TyrSer: 2.366 ± 0.055
2.428TyrThr: 2.428 ± 0.057
2.62TyrVal: 2.62 ± 0.054
0.353TyrTrp: 0.353 ± 0.018
1.909TyrTyr: 1.909 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3275 proteins (1061070 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski