Alces alces faeces associated microvirus MP10 5560
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CI91|A0A2Z5CI91_9VIRU Minor capsid protein OS=Alces alces faeces associated microvirus MP10 5560 OX=2219133 PE=4 SV=1
MM1 pKa = 7.42 NRR3 pKa = 11.84 NTQNHH8 pKa = 6.26 FATLPNVDD16 pKa = 3.07 ISRR19 pKa = 11.84 SRR21 pKa = 11.84 FKK23 pKa = 11.03 RR24 pKa = 11.84 PFTHH28 pKa = 6.16 KK29 pKa = 8.2 TTFNTGDD36 pKa = 4.6 IIPLMLQEE44 pKa = 4.83 ILPGDD49 pKa = 4.4 TVTMDD54 pKa = 3.28 MGAAIRR60 pKa = 11.84 MSTPIYY66 pKa = 10.2 PVMDD70 pKa = 3.55 NAFFDD75 pKa = 4.38 YY76 pKa = 11.32 YY77 pKa = 11.2 FFFVPNRR84 pKa = 11.84 LTWEE88 pKa = 3.57 HH89 pKa = 6.3 WEE91 pKa = 3.98 EE92 pKa = 4.2 FMGEE96 pKa = 3.99 NKK98 pKa = 7.68 QTAWEE103 pKa = 4.11 QKK105 pKa = 8.26 TEE107 pKa = 3.98 YY108 pKa = 10.18 EE109 pKa = 4.16 IPQIKK114 pKa = 10.1 APEE117 pKa = 4.51 GGWAKK122 pKa = 9.5 GTIADD127 pKa = 4.34 HH128 pKa = 6.12 MGIPTKK134 pKa = 10.74 VEE136 pKa = 4.28 NISVSALPFRR146 pKa = 11.84 AYY148 pKa = 10.64 GLIYY152 pKa = 10.65 NEE154 pKa = 4.1 WFRR157 pKa = 11.84 DD158 pKa = 3.68 QNLKK162 pKa = 10.31 DD163 pKa = 3.48 PCMVNTDD170 pKa = 3.95 DD171 pKa = 3.87 TTQTGSNSGDD181 pKa = 3.44 YY182 pKa = 10.81 VINTQLGALPCKK194 pKa = 9.26 AAKK197 pKa = 9.53 YY198 pKa = 9.96 HH199 pKa = 7.56 DD200 pKa = 5.05 YY201 pKa = 9.17 FTSALPEE208 pKa = 4.12 PQKK211 pKa = 11.29 GPDD214 pKa = 3.26 VLLPQGLTAPVKK226 pKa = 9.7 TSSEE230 pKa = 4.25 YY231 pKa = 10.88 AVTPEE236 pKa = 4.08 EE237 pKa = 4.78 VEE239 pKa = 4.33 TLPSLRR245 pKa = 11.84 WAGRR249 pKa = 11.84 GDD251 pKa = 4.12 DD252 pKa = 4.48 GVTTYY257 pKa = 10.03 TGSRR261 pKa = 11.84 RR262 pKa = 11.84 LVAYY266 pKa = 9.18 GQGATDD272 pKa = 3.52 YY273 pKa = 9.88 TMEE276 pKa = 4.35 GVTYY280 pKa = 10.2 LAGVGDD286 pKa = 4.2 STEE289 pKa = 4.27 DD290 pKa = 3.33 GQYY293 pKa = 10.9 FPINLQADD301 pKa = 4.26 LSMATGATINQLRR314 pKa = 11.84 QAFAVQKK321 pKa = 10.36 FYY323 pKa = 11.4 EE324 pKa = 4.21 KK325 pKa = 10.03 QARR328 pKa = 11.84 GGSRR332 pKa = 11.84 YY333 pKa = 9.63 RR334 pKa = 11.84 EE335 pKa = 4.46 TIRR338 pKa = 11.84 QMFGVSSPDD347 pKa = 2.78 ARR349 pKa = 11.84 MQIPEE354 pKa = 4.09 YY355 pKa = 10.55 LGGHH359 pKa = 5.7 RR360 pKa = 11.84 QPINIDD366 pKa = 3.25 QVLQTSATDD375 pKa = 3.44 TTSPQGNTAAYY386 pKa = 9.74 SLTNIRR392 pKa = 11.84 DD393 pKa = 3.8 SVFTKK398 pKa = 10.58 SFVEE402 pKa = 4.46 HH403 pKa = 6.94 GYY405 pKa = 11.03 LMCLGVVRR413 pKa = 11.84 TYY415 pKa = 9.42 HH416 pKa = 6.95 TYY418 pKa = 9.33 QQGIDD423 pKa = 3.5 AHH425 pKa = 6.12 WSRR428 pKa = 11.84 KK429 pKa = 6.98 TLFDD433 pKa = 3.98 FYY435 pKa = 11.44 FPVFANLGEE444 pKa = 3.9 QPIYY448 pKa = 10.81 NKK450 pKa = 10.2 EE451 pKa = 3.59 IYY453 pKa = 9.16 ATGTEE458 pKa = 3.86 TDD460 pKa = 3.8 EE461 pKa = 4.49 EE462 pKa = 4.91 VFGYY466 pKa = 9.86 QEE468 pKa = 3.0 AWAPYY473 pKa = 8.52 RR474 pKa = 11.84 YY475 pKa = 10.22 APARR479 pKa = 11.84 VSGAFRR485 pKa = 11.84 SNYY488 pKa = 8.98 EE489 pKa = 3.62 QTLDD493 pKa = 3.06 SWHH496 pKa = 6.04 YY497 pKa = 9.34 ADD499 pKa = 5.47 YY500 pKa = 11.38 YY501 pKa = 10.7 EE502 pKa = 4.49 EE503 pKa = 4.24 QPILSSDD510 pKa = 3.66 WIDD513 pKa = 3.27 EE514 pKa = 3.75 TRR516 pKa = 11.84 ANVNRR521 pKa = 11.84 TIAVQDD527 pKa = 3.58 EE528 pKa = 4.89 LEE530 pKa = 4.32 DD531 pKa = 3.62 QFIGDD536 pKa = 4.63 FYY538 pKa = 11.41 FAGDD542 pKa = 3.73 WVRR545 pKa = 11.84 PLPIYY550 pKa = 9.75 SIPGLIDD557 pKa = 2.67 HH558 pKa = 7.14 HH559 pKa = 6.66
Molecular weight: 63.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.682
IPC2_protein 4.8
IPC_protein 4.774
Toseland 4.647
ProMoST 4.876
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.622
Rodwell 4.635
Grimsley 4.571
Solomon 4.736
Lehninger 4.698
Nozaki 4.851
DTASelect 5.029
Thurlkill 4.647
EMBOSS 4.647
Sillero 4.914
Patrickios 3.363
IPC_peptide 4.749
IPC2_peptide 4.902
IPC2.peptide.svr19 4.864
Protein with the highest isoelectric point:
>tr|A0A2Z5CHZ8|A0A2Z5CHZ8_9VIRU Major capsid protein OS=Alces alces faeces associated microvirus MP10 5560 OX=2219133 PE=3 SV=1
MM1 pKa = 7.51 ACYY4 pKa = 9.69 HH5 pKa = 6.59 PLVAYY10 pKa = 8.07 EE11 pKa = 3.98 HH12 pKa = 6.56 TFLKK16 pKa = 10.18 TEE18 pKa = 3.99 NNKK21 pKa = 10.13 AVIKK25 pKa = 9.26 FQKK28 pKa = 9.98 PEE30 pKa = 3.67 QDD32 pKa = 3.74 EE33 pKa = 4.82 IYY35 pKa = 10.21 CGYY38 pKa = 9.12 WKK40 pKa = 9.17 TVEE43 pKa = 4.67 LPCGTCDD50 pKa = 3.59 GCKK53 pKa = 10.12 LEE55 pKa = 4.87 HH56 pKa = 6.49 AKK58 pKa = 10.29 NWAIRR63 pKa = 11.84 CMLEE67 pKa = 3.72 AKK69 pKa = 10.1 LYY71 pKa = 7.9 KK72 pKa = 10.0 HH73 pKa = 6.61 NYY75 pKa = 9.51 FITLTYY81 pKa = 10.72 AEE83 pKa = 4.75 EE84 pKa = 4.14 NVPTIAYY91 pKa = 8.7 TDD93 pKa = 3.37 ISDD96 pKa = 3.58 KK97 pKa = 11.44 DD98 pKa = 3.39 NYY100 pKa = 10.02 QYY102 pKa = 11.22 CEE104 pKa = 4.05 EE105 pKa = 4.16 LTLCKK110 pKa = 9.76 RR111 pKa = 11.84 DD112 pKa = 3.39 FQLFMKK118 pKa = 10.47 RR119 pKa = 11.84 LRR121 pKa = 11.84 KK122 pKa = 9.17 FYY124 pKa = 10.3 KK125 pKa = 10.09 KK126 pKa = 10.09 KK127 pKa = 10.83 YY128 pKa = 8.02 KK129 pKa = 9.11 WDD131 pKa = 3.93 NIRR134 pKa = 11.84 FFACGEE140 pKa = 4.02 YY141 pKa = 10.64 GDD143 pKa = 3.96 RR144 pKa = 11.84 TGRR147 pKa = 11.84 PHH149 pKa = 4.86 YY150 pKa = 10.12 HH151 pKa = 7.01 AIIFNLPINDD161 pKa = 4.27 LKK163 pKa = 11.08 PYY165 pKa = 10.06 FINEE169 pKa = 4.05 LHH171 pKa = 5.0 QQIYY175 pKa = 9.59 QSEE178 pKa = 4.66 TISNIWGLGLVTIGEE193 pKa = 4.39 VTYY196 pKa = 10.88 EE197 pKa = 4.01 SAGYY201 pKa = 8.0 VARR204 pKa = 11.84 YY205 pKa = 6.12 TLKK208 pKa = 10.64 KK209 pKa = 9.03 RR210 pKa = 11.84 TKK212 pKa = 10.2 QEE214 pKa = 4.19 TIDD217 pKa = 3.57 KK218 pKa = 10.33 QGEE221 pKa = 4.28 KK222 pKa = 10.46 EE223 pKa = 3.93 FTLMSRR229 pKa = 11.84 KK230 pKa = 9.54 PGIAQKK236 pKa = 10.92 YY237 pKa = 5.86 YY238 pKa = 8.72 TDD240 pKa = 3.78 NVEE243 pKa = 4.94 KK244 pKa = 10.1 IYY246 pKa = 11.14 KK247 pKa = 9.04 NDD249 pKa = 3.66 EE250 pKa = 4.39 IIISTSLDD258 pKa = 3.4 YY259 pKa = 11.0 VLKK262 pKa = 10.46 SKK264 pKa = 10.34 PPRR267 pKa = 11.84 YY268 pKa = 9.09 FDD270 pKa = 3.69 KK271 pKa = 11.14 LYY273 pKa = 10.83 DD274 pKa = 3.65 IDD276 pKa = 4.97 NEE278 pKa = 3.78 EE279 pKa = 4.13 DD280 pKa = 3.85 LYY282 pKa = 11.72 NKK284 pKa = 9.9 KK285 pKa = 8.78 QEE287 pKa = 4.09 RR288 pKa = 11.84 RR289 pKa = 11.84 KK290 pKa = 9.83 KK291 pKa = 9.26 AQEE294 pKa = 3.7 KK295 pKa = 10.06 RR296 pKa = 11.84 DD297 pKa = 3.72 YY298 pKa = 8.92 QQKK301 pKa = 8.36 HH302 pKa = 3.75 TTNRR306 pKa = 11.84 PDD308 pKa = 3.56 EE309 pKa = 4.15 LLKK312 pKa = 10.38 IAEE315 pKa = 4.28 RR316 pKa = 11.84 KK317 pKa = 8.76 KK318 pKa = 9.9 QQSTKK323 pKa = 10.91 ALNRR327 pKa = 11.84 QLKK330 pKa = 10.37 DD331 pKa = 2.96 II332 pKa = 4.62
Molecular weight: 39.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.187
IPC2_protein 8.229
IPC_protein 8.083
Toseland 8.404
ProMoST 8.609
Dawson 8.931
Bjellqvist 8.96
Wikipedia 9.033
Rodwell 9.048
Grimsley 8.682
Solomon 9.004
Lehninger 8.975
Nozaki 9.048
DTASelect 8.814
Thurlkill 8.887
EMBOSS 9.077
Sillero 9.107
Patrickios 4.164
IPC_peptide 8.99
IPC2_peptide 7.673
IPC2.peptide.svr19 7.72
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1531
84
559
255.2
29.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.903 ± 1.64
0.98 ± 0.47
5.356 ± 0.681
7.969 ± 1.121
4.572 ± 0.365
6.532 ± 0.894
2.09 ± 0.385
6.466 ± 0.731
7.381 ± 2.136
6.14 ± 0.522
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.809 ± 0.673
5.683 ± 0.78
4.115 ± 0.75
5.487 ± 0.838
3.919 ± 0.666
4.768 ± 0.733
7.577 ± 0.68
3.396 ± 0.727
1.437 ± 0.286
5.421 ± 0.868
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here